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Research
Amoeba-Resisting Bacteria
and Ventilator-Associated Pneumonia
Bernard La Scola,* Ioanna Boyadjiev,*† Gilbert Greub,* Atieh Khamis,*
Claude Martin,† and Didier Raoult*†
*Unité des Rickettsies, Marseille, France; and †Hôpital Nord, Marseille,
France
| Table 2. Polymerase
chain reaction primers used for amplification and sequencing of bacterial
DNA within human samples |
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Primers
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Bosea-related strain
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Legionella anisa
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Afipia broomeae
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L. quinlivanii
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External forward
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5´-TGCGAGTGTAGAGGTGAAATT-3´
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5´-TATTGGTGCTGATTTAGGAA-3´
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5´-TCTTTTGTGCGGGAAGATAATG-3´
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5´-TTGTTGATGTTTGTTTTGAGACC-3´
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External reverse
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5´CGCTCGTTGCGGGACTTAA-3´
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5´-GCTAAGTCTGAAGGTACA-3´
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5´-TAAACTTTCCAACGGCTGGCAT-3´
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5´-TTCAACACTTCTTTCATCTGATC-3´
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Internal forward
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5´-GAGGTGAAATTCGTAGATATT-3´
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5´-GCCCAATTGATTTTGACAG-3´
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5´-GCTAACTTCGTGCCAGCAG-3´
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5´-TCCAAGAATAAAAGGGGATTG-3´
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Internal reverse
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5´-GAGCTGACGACAGCCAT-3´
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5´-GCATTAATTGTAATGCTTCA-3´
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5´-GTTTGCTCCCCACGCTTTC-3´
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5´-CCATACCATCCTGTAAGCCTT-3´
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