Volume 12, Number 6, June 2006

Co-infections of Adenovirus Species in Previously Vaccinated Patients

Gary J. Vora,*† Baochuan Lin,*† Kevin Gratwick,‡ Carolyn Meador,§ Christian Hansen,‡ Clark Tibbetts,† David A. Stenger,*† Marina Irvine,‡ Donald Seto,†¶ Anjan Purkayastha,†¶ Nikki E. Freed,‡ Marylou G. Gibson,# Kevin Russell,†‡ and David Metzgar†‡
*Naval Research Laboratory, Washington, DC, USA; †Epidemic Outbreak Surveillance Consortium, Falls Church, Virginia, USA; ‡Naval Health Research Center, San Diego, California, USA; §Nova Research Inc., Alexandria, Virginia, USA; ¶George Mason University, Manassas, Virginia, USA; and #Virapur, LLC, San Diego, California, USA

Figure. Molecular methods used to identify human adenovirus (HAdV) co-infections. A–D) Vaccinated sample 7274. A) Microarray hybridization profile. White and yellow rectangles indicate low-positive HAdV-C and HAdV-B2 targets, respectively. Spot colors denote hybridization signal intensity (white > yellow > green > blue). Species and corresponding serotype designations are indicated on the left. Probe designations (E1, E2 = serotype-specific E1A probes; H1, H2 = serotype-specific hexon probes; F1, F2 = serotype-specific fiber probes) are indicated above each array. B) Adenovirus Consensus kit optical density values. *, amplification positive. The horizontal line is the manufacturer's significance threshhold. C) Multiplex species-specific polymerase chain reaction (PCR). m, molecular mass marker. Species designations are to the right of the corresponding band. D) PCR verification with independent serotype or species-specific primers. E–H) Unvaccinated sample 10756. E) Microarray hybridization profile. F) Adenovirus Consensus kit optical density values. G) Multiplex species-specific PCR. H) PCR verification with independent serotype-specific primers.

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This page last reviewed April 21, 2006

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
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