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Figure. A) Schematic representation of the genome of Nipah virus
(NV). Negative-sense genomic RNA is shown in 3´ to 5´ orientation.
Open reading frames (ORFs) are indicated by shaded boxes: N, nucleoprotein;
P, phosphoprotein; M, matrix protein; F, fusion protein; G, attachment
protein; L, polymerase protein. B) Phylogenetic analysis of the N ORFs
from members of the subfamily Paramyxovirinae. Arrows identify
the 5 genera. A phenogram of the N ORFs of members of this subfamily was
created by using maximum parsimony analysis with PAUP 4.02 (Sinauer Associates,
Sunderland, MA, USA). Abbreviations and accession numbers: HPIV-1, human
parainfluenza virus, D01070; Sendai, X00087; HPIV-3, D10025; CDV, canine
distemper virus, AF014953; PDV, phocine distemper virus, X75717; RPV,
Rinderpest virus, X68311; MV, K01711; DMV, dolphin Morbillivirus, X75961;
NDV, Newcastle disease virus, AF064091; GP, goose paramyxovirus, AF473851;
HPIV-4b, M32983; HPIV-4a, M32982; Tioman, AF298895; Menangle, AF326114;
HPIV-2, M55320; Simian virus 5 (SV5), M81442; Mumps, D86172; NV-UMCC1,
AY029767; NV-Malaysia, AF212302; NV-P. hypomelanus,* AF376747; NV-Bangladesh;
Hendra virus, AF017149; Tupaia paramyxovirus, AF079780; Mossman virus,
AY286409; and Salem virus, AF237881. C) The phylogenetic relationship
between the N gene sequences of the 4 human NV isolates from the Bangladesh
outbreak in 2004 and the N gene sequences from pig and human NV isolates
from Malaysia. Accession numbers for the pig isolates of NV (13)
are AJ627196, AJ564622, and AJ564621. *NV-P. hypomelanus is sequence
from a virus isolated from Pteropus hypomelanus, the Island Flying
Fox (8).
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