Search Search Status Download FAQ Help   File name: File type: blank line delimited DTA XML encapsulated DTA PKL format MGF format Enzyme: Trypsin Arg-C CNBr Chymotrypsin Formic Acid Lys-C Lys-C, no P rule Pepsin A Trypsin+CNBr Trypsin+Chymotrypsin Trypsin, no P rule Whole protein Asp-N Glu-C Asp-N+Glu-C Top-Down Semi-Tryptic No Enzyme Chymotrypsin, no P rule Asp-N (DE) Glu-C (DE) Maximum missed cleavages: 0 1 2 3 Sequence library: refseq_protein nr swissprot Species to search (ctrl key for multiple selection): Homo sapiens (human) Mus musculus (mouse) Saccharomyces cerevisiae (yeast) Aeropyrum pernix Agrobacterium tumefaciens Anopheles gambiae Aquifex aeolicus Arabidopsis thaliana Bacillus subtilis Borrelia burgdorferi Bos taurus (cow) Caenorhabditis elegans Campylobacter jejuni Canis familiaris (dog) Chlamydomonas reinhardtii Danio rerio Dictyostelium discoideum Drosophila melanogaster Equus caballus (horse) Escherichia coli Gallus gallus (chicken) Giardia lamblia ATCC 50803 Hordeum vulgare (barley) L. interrogans serovar Lai Magnaporthe grisea Methanococcus jannaschii Mycobacterium smegmatis Mycobacterium tuberculosis Mycoplasma genitalium Neurospora crassa Oryctolagus cuniculus Oryza sativa Ovis aries Plasmodium falciparum Pneumocystis carinii Rattus norvegicus (rat) Salmonella enterica Schizosaccharomyces pombe Solanum tuberosum (potato) Streptococcus pneumoniae Streptomyces coelicolor Sus scrofa Synechocystis PCC6803 Takifugu rubripes Triticum aestivum (wheat) Trypanosoma cruzi strain CL Brener Toxoplasma gondii Xenopus laevis Xenopus tropicalis Zea mays Hitlist max length: 10 25 50 E-value cutoff: 50 25 10 1 0.1 0.01 0.001 0.0001 Fixed mods (ctrl key for multiple selection): 2-amino-3-oxo-butanoic acid T Asparagine HexNAc Asparagine dHexHexNAc CAMthiopropanoyl K ICAT heavy ICAT light M cleavage from protein n-term MMTS on C NEM C NIPCAM O18 on peptide n-term PNGasF in O18 water SeMet Serine HexNAc Threonine HexNAc acetylation of K acetylation of protein n-term amidation of peptide c-term arginine to ornithine beta elimination of S beta elimination of T beta methythiolation of D beta-methylthiolation of D carbamidomethyl C carbamylation of K carbamylation of n-term peptide carboxyamidomethylation of D carboxyamidomethylation of E carboxyamidomethylation of H carboxyamidomethylation of K carboxykynurenin of W carboxymethyl C citrullination of R deamidation of N and Q dehydro of S and T di-O18 on peptide n-term di-iodination of Y di-methylation of K di-methylation of R di-methylation of peptide n-term farnesylation of C fluorophenylalanine formylation of K formylation of peptide n-term formylation of protein n-term gamma-carboxylation of D gamma-carboxylation of E geranyl-geranyl glucuronylation of protein n-term glutathione disulfide guanidination of K heavy arginine-13C6 heavy arginine-13C6-15N4 heavy lysine - 13C6 15N2 heavy lysine - 2H4 heavy lysine-13C6 homoserine homoserine lactone hydroxylation of Y hydroxylation of D hydroxylation of F hydroxylation of K hydroxylation of N hydroxylation of P iTRAQ114 on K iTRAQ114 on Y iTRAQ114 on nterm iTRAQ115 on K iTRAQ115 on Y iTRAQ115 on nterm iTRAQ116 on K iTRAQ116 on Y iTRAQ116 on nterm iTRAQ117 on K iTRAQ117 on Y iTRAQ117 on nterm iodination of Y lipoyl K methyl C methyl H methyl N methyl R methyl ester of D methyl ester of E methyl ester of S methyl ester of Y methyl ester of peptide c-term methylation of D methylation of E methylation of K methylation of Q methylation of peptide c-term methylation of peptide n-term methylation of protein n-term myristoleylation of G myristoyl-4H of G myristoylation of K myristoylation of peptide n-term G n-acyl diglyceride cysteine n-formyl met addition oxidation of C to cysteic acid oxidation of C to sulfinic acid oxidation of F to dihydroxyphenylalanine oxidation of H oxidation of H to D oxidation of H to N oxidation of M oxidation of P to pyroglutamic acid oxidation of W oxidation of W to formylkynurenin oxidation of W to hydroxykynurenin oxidation of W to kynurenin oxidation of W to nitro oxidation of Y to nitro palmitoylation of C palmitoylation of K palmitoylation of S palmitoylation of T phosphopantetheine S phosphorylation of S phosphorylation of S with ETD loss phosphorylation of S with prompt loss phosphorylation of T phosphorylation of T with ETD loss phosphorylation of T with prompt loss phosphorylation of Y phosphorylation with neutral loss on C phosphorylation with neutral loss on D phosphorylation with neutral loss on H phosphorylation with neutral loss on S phosphorylation with neutral loss on T phosphorylation with prompt loss on Y propionamide C propionyl heavy K propionyl heavy peptide n-term propionyl light K propionyl light on peptide n-term pyridyl K pyridyl peptide n-term pyro-cmC pyro-glu from n-term E pyro-glu from n-term Q s-pyridylethylation of C sulfation of Y sulphone of M sumoylation of K thioacylation of K thioacylation of peptide n-term tri-deuteromethylation of D tri-deuteromethylation of E tri-deuteromethylation of peptide c-term tri-iodination of Y tri-methylation of K tri-methylation of R tri-methylation of protein n-term ubiquitinylation residue Variable mods (ctrl key for multiple selection): 2-amino-3-oxo-butanoic acid T Asparagine HexNAc Asparagine dHexHexNAc CAMthiopropanoyl K ICAT heavy ICAT light M cleavage from protein n-term MMTS on C NEM C NIPCAM O18 on peptide n-term PNGasF in O18 water SeMet Serine HexNAc Threonine HexNAc acetylation of K acetylation of protein n-term amidation of peptide c-term arginine to ornithine beta elimination of S beta elimination of T beta methythiolation of D beta-methylthiolation of D carbamidomethyl C carbamylation of K carbamylation of n-term peptide carboxyamidomethylation of D carboxyamidomethylation of E carboxyamidomethylation of H carboxyamidomethylation of K carboxykynurenin of W carboxymethyl C citrullination of R deamidation of N and Q dehydro of S and T di-O18 on peptide n-term di-iodination of Y di-methylation of K di-methylation of R di-methylation of peptide n-term farnesylation of C fluorophenylalanine formylation of K formylation of peptide n-term formylation of protein n-term gamma-carboxylation of D gamma-carboxylation of E geranyl-geranyl glucuronylation of protein n-term glutathione disulfide guanidination of K heavy arginine-13C6 heavy arginine-13C6-15N4 heavy lysine - 13C6 15N2 heavy lysine - 2H4 heavy lysine-13C6 homoserine homoserine lactone hydroxylation of Y hydroxylation of D hydroxylation of F hydroxylation of K hydroxylation of N hydroxylation of P iTRAQ114 on K iTRAQ114 on Y iTRAQ114 on nterm iTRAQ115 on K iTRAQ115 on Y iTRAQ115 on nterm iTRAQ116 on K iTRAQ116 on Y iTRAQ116 on nterm iTRAQ117 on K iTRAQ117 on Y iTRAQ117 on nterm iodination of Y lipoyl K methyl C methyl H methyl N methyl R methyl ester of D methyl ester of E methyl ester of S methyl ester of Y methyl ester of peptide c-term methylation of D methylation of E methylation of K methylation of Q methylation of peptide c-term methylation of peptide n-term methylation of protein n-term myristoleylation of G myristoyl-4H of G myristoylation of K myristoylation of peptide n-term G n-acyl diglyceride cysteine n-formyl met addition oxidation of C to cysteic acid oxidation of C to sulfinic acid oxidation of F to dihydroxyphenylalanine oxidation of H oxidation of H to D oxidation of H to N oxidation of M oxidation of P to pyroglutamic acid oxidation of W oxidation of W to formylkynurenin oxidation of W to hydroxykynurenin oxidation of W to kynurenin oxidation of W to nitro oxidation of Y to nitro palmitoylation of C palmitoylation of K palmitoylation of S palmitoylation of T phosphopantetheine S phosphorylation of S phosphorylation of S with ETD loss phosphorylation of S with prompt loss phosphorylation of T phosphorylation of T with ETD loss phosphorylation of T with prompt loss phosphorylation of Y phosphorylation with neutral loss on C phosphorylation with neutral loss on D phosphorylation with neutral loss on H phosphorylation with neutral loss on S phosphorylation with neutral loss on T phosphorylation with prompt loss on Y propionamide C propionyl heavy K propionyl heavy peptide n-term propionyl light K propionyl light on peptide n-term pyridyl K pyridyl peptide n-term pyro-cmC pyro-glu from n-term E pyro-glu from n-term Q s-pyridylethylation of C sulfation of Y sulphone of M sumoylation of K thioacylation of K thioacylation of peptide n-term tri-deuteromethylation of D tri-deuteromethylation of E tri-deuteromethylation of peptide c-term tri-iodination of Y tri-methylation of K tri-methylation of R tri-methylation of protein n-term ubiquitinylation residue Maximum variable mod combinations searched per peptide:1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 Precursor mass tolerance (Da): Product mass tolerance (Da): Precursor mass search type: monoisotopic average monoisotopic N15 exact Product mass search type: monoisotopic average monoisotopic N15 exact Lower bound of precursor charge: 1 2 3 4 5 6 7 8 9 Upper bound of precursor charge: 1 2 3 4 5 6 7 8 9 Minimum charge to start using multiply charged products:1 2 3 4 5 6 7 8 9 Fraction of product peaks below precursor to determine +1 precursor: Peak intensity cutoff: (fraction of most intense) Number of top intensity peaks in first pass: 3 6 20 Ions to search 1: a b c x y z p n i u Ions to search 2: a b c x y z p n i u   Write to the Help Desk | Disclaimer | Privacy statement | Accessibility |