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Optical Imaging and Instrumentation
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BIMI has a wide range of experience in applying lasers and optics to biomedical and clinical problems. Past projects have included development of laser capture microdissection, a confocal cDNA microarray scanner, and instrumentation for intraoperative photodynamic therapy. Below, we present a sampling of current areas of interest.
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| Infrared imaging for intraoperative monitoring |
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Infrared imaging can be used to monitor organ function in real time. At right, intraoperative monitoring of kidney circulation during transplant, before and after reperfusion.
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| Noninvasive optical imaging of tissue |
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Tissue absorbs weakly in the near-infrared (NIR) spectral region. By using specialized NIR light sources and highly sensitive imaging equipment, deeply embedded (> 1cm) fluorophores can be noninvasively detected.
When computational methods, developed by collaborators in NICHD, are used to account for light scattering and absorption, the fluorophore position can be determined from the surface intensity profile.
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| Laser-induced fluorescence detection in microchannels and capillaries |
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Solid state lasers and ball lens coupled fiber optics are used to make an LIF detection system with no moving parts.
The detector can be used for simultaneous, spectrally resolved, LIF detection from eight microfluidic channels in a single plastic chip, developed at NIST. This device is designed for four-color DNA separations, though the detector has a wide variety of potential applications.
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| Laser-induced fluorescence detection in capillary systems |
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Together with researchers in UAIR, we have developed a on-line LIF detector for capillary electrophoresis, capable of detecting sub-pg/mL protein concentrations in 50 nL sample volumes. A two-color system, which would permit simultaneous calibration with a labeled standard, is under development.
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| IRD Staff Specialists
Contact IRD staff for more information
Paul Smith, Ph.D. - IRD Chief, Research Physicist
Alexander Gorbach, Ph.D. - Staff Scientist
Nicole Morgan, Ph.D. - Staff Scientist
Ed Wellner, Biomedical Engineering Technician
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