Spectrophotofluorometer
This research tool combines the technologies
of the spectrometer, the photomultiplier tube,
and the fluorometer to provide sensitive measurements
of the color spread, or spectrum, of fluorescent
light in a sample. The instrument counts the
amount of light given off in each color band
and can be used to trace and measure tiny
components of a sample of blood, body tissue,
or other substance.
The
early AMINCO-Bowman SPF used two monochromators,
one to isolate a narrow band of light for
excitation and the other at right angles
to the first to analyze the emitted fluorescence
by measuring the intensity of the fluorescent
light vs. wavelength. For its excitation
energy, the SPF used a xenon-arc
lamp, which emitted a range of white
light emission from the visible well into
the ultraviolet.
Spectrophotofluorometer
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Diagram
of the spectrophotofluorometer.
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