Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
European John Dory  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Zeus faber
FDA Seafood List Common Name: European John Dory
FDA Seafood List Market Name: Dory
Unique IEF Gel Code: 19950809gel14
Unique Web Code: zsg01Srr
Tissue Aliquot Code: zeusfabe I94-002 #01a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel ImageCalibrated Comparison Gel Image
Gel ImageFish ImageCalibrated GelComparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5393.40.153606164.2408516.412.83.543.615.60.153332
24.5518115.70.26892341.84957.25.64.55181.54.10.251456
35.2265230.31607142.243458.76.85.2265.14.20.30358
45.8541936.40.48392232.237078.76.75.85419.52.50.49764
56.5558550.80.25592452.0732786.36.55583.45.20.225108
66.8562454.20.14404261.375875.34.16.85624.46.10.127413
77.3571662.20.21396772.514679.87.67.35714.17.40.191743
88.1587676.10.0843024111.528655.94.68.15876.710.70.0804527
98.4593881.50.15435971.8147375.58.459386.60.154738
108.6597784.90.16813961.660736.458.65977.55.70.16436
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3111596.90.46852464.2419316.512.89.31114.360.4002
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 7
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.11512510.80.099375850.7939632.424.115125.24.60.0979455
24.38516013.90.63572655.6271117.113.94.385160.44.80.655407
34.73720617.90.096473530.4761911.41.24.737205.530.0893513
44.81421618.70.17014641.3117843.24.814215.84.30.170414
55.576357310.40690521.423014.33.55.576356.51.90.42093
65.87642937.20.044709120.7444392.31.85.876432.711.70.0359549
75.96144938.90.12514810005.96144410
85.9945639.50.74434622.996359.17.45.99456.22.30.725676
96.0847741.40.43020752.995349.17.46.08478.24.60.367974
106.161496430.050478620.1902050.60.56.1614962.20.0481928
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.1950343.60.095376220.31900210.86.19501.71.70.104732
126.21650944.10.48274243.4166910.48.46.216510.340.48789
136.39555147.80.150632133.5151810.78.76.395549.612.70.155901
146.48457249.60.089799381.060133.22.66.484570.77.70.0779692
156.69860752.60.268735104.9882115.112.36.698604.69.50.295543
167.326715620.013156860.1846390.60.57.326716.66.40.0163394
177.44773964.10.042242970.5455551.71.37.447738.97.20.0427895
187.724795690.1483102.372347.25.87.724792.99.60.139814
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 8
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5484.20.145742195.0965117.513.43.549.118.90.152439
24.5517915.50.30130441.780976.14.74.55179.53.60.275799
35.2265230.33364642.058237.15.45.2265.13.70.31399
45.8542436.80.43477432.595558.96.85.85423.93.30.450006
56.5558750.90.24768362.079647.15.56.55585.85.50.213159
66.8562754.40.16425961.576285.44.16.85627.26.10.145149
77.3572062.40.20091692.676479.277.35717.28.60.174697
88.1588376.60.0889015101.561635.44.18.15882.910.10.0875571
98.4594481.90.18394772.255527.75.98.45943.37.10.179485
108.6598285.20.18283761.943486.75.18.65982.16.20.178076
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.31118970.53412475.4986518.914.59.31117.56.80.456575
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 9
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5554.80.177984206.4733411.810.43.558.520.30.179512
24.02912610.90.0870260.803411.51.34.029126.95.60.0806452
34.31216414.20.55929854.659038.57.54.3121644.50.57967
44.55196170.31239642.065993.83.34.55195.94.30.269578
54.65521018.20.098983230.5164080.90.84.655210.33.10.0940025
64.74422219.30.14925441.034491.91.74.744222.33.90.150886
74.95325021.70.037607370.402320.70.64.9532487.10.0320851
85.228324.50.30525541.928523.53.15.2283.33.70.290703
95.5436131.30.28619721.185582.21.95.54360.62.30.291614
105.78941836.30.06615470.7481471.41.25.789417.16.70.0627091
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.8543237.50.3491831.96593.63.25.85431.83.10.354888
125.95745639.50.13526420.6348031.215.957456.92.10.173035
135.98846340.20.62483633.136125.755.988463.72.90.618241
146.08648542.10.29662131.282342.32.16.086485.12.60.283471
156.12649442.80.12037951.162172.11.96.126492.14.50.144431
166.23351844.90.39351953.42916.25.56.233519.34.90.396543
176.45656849.30.111571153.132195.756.456573.214.60.120856
186.5558951.10.1147350.6098561.116.55587.94.80.0722577
196.715611530.32653563.347826.15.46.715613.86.10.311355
206.8562954.60.12543992.022423.73.26.856269.30.122863
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.3572262.60.20584262.153443.93.57.35720.76.40.188499
227.49875265.20.024953670.2734130.50.47.498751.56.80.0227197
237.77807700.0833917242.935.34.77.77799.9240.0688796
248.1588476.70.035752190.5332510.98.15883.68.90.0336656
258.4594381.80.14952771.921543.53.18.45943.57.30.149372
268.6598485.30.15356771.764963.22.88.65983.46.60.150178
279.3111997.10.49727164.867368.97.89.31118.55.90.468259
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.04412410.80.10766350.9532262.92.44.044124.45.30.100635
24.33116214.10.64720355.6961817.614.14.331162.14.80.672775
34.68620918.10.10504420.4443231.41.14.686208.52.40.105493
44.76922019.10.18160541.255243.93.14.769220.23.90.183233
54.98124821.50.026484460.2660020.80.74.9812466.20.0242273
65.45233629.20.042559350.3463611.10.95.452334.95.10.0380901
75.56336231.40.41680221.494274.63.75.563362.41.90.432358
85.78641435.90.033709140.2290320.70.65.786413.13.50.0368144
95.85443037.30.16904330.895712.82.25.854429.63.30.152817
105.97945939.80.093242410005.97945310
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
11646440.30.65472722.765358.66.96464.62.30.672637
126.09548642.20.41698843.071919.57.66.095486.74.40.39059
136.19450944.20.067915630.31255610.86.194508.72.60.0670493
146.232518450.42584143.24542108.16.232519.14.20.431429
156.37855247.90.18760372.341967.25.86.378552.87.50.176722
166.46857349.70.0684194131.777125.54.46.468568.313.20.075918
176.64160452.40.36234174.4396313.7116.641607.47.40.337432
187.31871962.40.024361850.2758020.90.77.318720.95.40.0286188
197.41473864.10.052567570.63239121.67.414739.47.20.0497387
207.71279869.30.114338101.900755.94.77.712794.810.30.103935
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5494.30.169128195.9098120.215.73.55019.40.17182
24.5518616.10.31671342.014686.95.44.55186.340.283418
35.227123.50.32452642.078237.15.55.2271.13.80.306299
45.85426370.48382232.404568.26.45.85425.92.80.489513
56.5559551.60.22680162.226777.65.96.55592.66.50.194092
66.85634550.14383661.387884.73.76.856356.10.128699
77.3572863.20.19937672.390058.26.47.35725.57.40.181614
88.1588977.20.0993821101.751364.78.15889.110.50.0944889
98.4594882.30.17933872.145367.35.78.459476.80.178603
108.6598485.40.16457861.76996.14.78.65984.66.20.161916
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.31118970.51301275.1537917.613.79.31116.670.412465
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic

 
Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 98
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 7
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 8
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 9
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse   |   RFE Tutorial   |   Seafoods   |   CFSAN


Last updated on 2003-FEB-27 by bjt/frf/nln