RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19930812gel03
Unique Web Code: ysh03Srr
Tissue Aliquot Code: ocyuchry 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	100	11.7	0.0646079	8	0.83125	8.9	7.1	4.55	100.4	8.2	0.0570695
2	5.2	197	23	0.10986	4	0.761126	8.1	6.5	5.2	197.1	3.9	0.109291
3	5.85	301	35.1	0.132923	5	1.09837	11.8	9.4	5.85	301.1	4.7	0.131461
4	6.55	442	51.6	0.0892859	8	1.16705	12.5	10	6.55	440.9	7.6	0.0864335
5	6.85	473	55.2	0.0113489	8	0.203371	2.2	1.7	6.85	475.2	7.9	0.0145424
6	7.35	536	62.5	0.0468904	6	0.518349	5.5	4.5	7.35	536.9	6.1	0.0479629
7	8.15	660	77	0.100331	8	1.33237	14.3	11.4	8.15	659.5	7.8	0.096259
8	8.45	726	84.7	0.091016	8	1.20735	12.9	10.4	8.45	725.1	7.5	0.0903055
9	8.65	778	90.8	0.0475039	6	0.520262	5.6	4.5	8.65	777.7	6.4	0.0460529
10	9.3	825	96.3	0.152335	7	1.70546	18.3	14.7	9.3	824.7	6.8	0.140935

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.195	47	5.5	0.127524	7	1.6656	6.5	5.8	4.195	47.7	7.4	0.127155
2	4.55	101	11.8	0.0356886	5	0.321301	1.3	1.1	4.55	102.1	5.4	0.0337622
3	4.694	123	14.4	0.0111168	3	0.0567618	0.2	0.2	4.694	123.2	2.7	0.0116987
4	4.793	138	16.2	0.274727	5	2.28058	8.9	7.9	4.793	137.6	4.8	0.268858
5	4.937	160	18.8	0.0580949	12	1.24001	4.9	4.3	4.937	156.1	12.3	0.0566503
6	5.2	200	23.4	0.044068	5	0.331371	1.3	1.1	5.2	199.1	4.6	0.0410698
7	5.349	224	26.3	0.0159797	5	0.134581	0.5	0.5	5.349	224.2	4.6	0.0164461
8	5.429	237	27.8	0.0659212	3	0.364818	1.4	1.3	5.429	237.1	3.2	0.0642267
9	5.646	272	31.9	0.0175713	4	0.131337	0.5	0.5	5.646	271.2	4.4	0.0168411
10	5.85	305	35.8	0.0625129	5	0.471469	1.8	1.6	5.85	305	4.5	0.0586804
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.978	330	38.7	0.498341	3	2.90871	11.4	10.1	5.978	330	3.4	0.484595
12	6.06	346	40.6	0.256136	4	1.67914	6.6	5.8	6.06	345.7	3.8	0.247439
13	6.142	362	42.4	0.113938	4	0.797982	3.1	2.8	6.142	361.9	4.1	0.109974
14	6.188	371	43.5	0.113454	4	0.699902	2.7	2.4	6.188	370.6	3.7	0.106837
15	6.485	429	50.3	0.102716	6	1.09318	4.3	3.8	6.485	429	6.3	0.0980913
16	6.563	443	51.9	0.064722	2	0.184592	0.7	0.6	6.563	443	1.8	0.0579998
17	6.641	451	52.9	0.43539	2	1.57421	6.2	5.4	6.641	451.4	2	0.445625
18	6.729	460	53.9	0.101074	5	0.97761	3.8	3.4	6.729	459.8	5.2	0.106143
19	6.837	471	55.2	0.121893	4	0.821489	3.2	2.8	6.837	471.2	4.2	0.109782
20	6.927	482	56.5	0.0696704	7	0.806072	3.2	2.8	6.927	481.2	6.9	0.0656887
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.079	501	58.7	0.214195	4	1.40626	5.5	4.9	7.079	500.9	3.8	0.207333
22	7.143	509	59.7	0.485615	4	3.2239	12.6	11.1	7.143	508.8	3.7	0.489766
23	7.254	523	61.3	0.0418459	3	0.241561	0.9	0.8	7.254	523.1	3	0.0447578
24	7.35	535	62.7	0.0147838	5	0.122843	0.5	0.4	7.35	535.5	4.9	0.0142842
25	8.15	659	77.3	0.0371674	6	0.397518	1.6	1.4	8.15	659.9	6.1	0.0370208
26	8.45	727	85.2	0.0321751	6	0.324974	1.3	1.1	8.45	726.5	5.7	0.0320732
27	8.542	749	87.8	0.0099466	5	0.0991784	0.4	0.3	8.542	747.6	5.1	0.0108696
28	8.65	775	90.9	0.0433906	5	0.376428	1.5	1.3	8.65	774.7	4.9	0.0429961
29	8.85	791	92.7	0.0214036	8	0.315073	1.2	1.1	8.85	790.8	8	0.0220946
30	9.3	827	97	0.0620935	4	0.445821	1.7	1.5	9.3	826.4	3.8	0.0663001

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.206	50	5.8	0.163667	8	2.12024	8	6.9	4.206	51.2	7.5	0.158585
2	4.569	106	12.4	0.0183215	8	0.216313	0.8	0.7	4.569	105.9	7.6	0.0161119
3	4.719	129	15.1	0.0173215	3	0.0740593	0.3	0.2	4.719	128.2	2.6	0.0162512
4	4.81	143	16.7	0.343982	5	2.9486	11.2	9.6	4.81	143.5	5	0.334748
5	4.972	168	19.6	0.0847873	13	1.79273	6.8	5.8	4.972	164.8	12.7	0.0796314
6	5.379	232	27.1	0.0239902	4	0.193873	0.7	0.6	5.379	232.2	4.2	0.0259082
7	5.453	244	28.5	0.0901664	3	0.465378	1.8	1.5	5.453	244.7	3	0.0885385
8	5.662	278	32.4	0.0343713	5	0.279673	1.1	0.9	5.662	278	4.7	0.0335032
9	5.863	311	36.3	0.0491249	4	0.333444	1.3	1.1	5.863	311.8	4.3	0.0438434
10	5.982	334	39	0.57336	3	3.18238	12.1	10.4	5.982	334.4	3.2	0.562032
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.065	350	40.8	0.294601	4	1.86158	7.1	6.1	6.065	350	3.7	0.283323
12	6.149	366	42.7	0.127194	4	0.88725	3.4	2.9	6.149	365.5	4.1	0.122057
13	6.19	374	43.6	0.13131	4	0.879274	3.3	2.9	6.19	374.1	4.1	0.12086
14	6.491	432	50.4	0.131713	6	1.26442	4.8	4.1	6.491	432.4	5.6	0.127595
15	6.566	445	51.9	0.0770972	2	0.247335	0.9	0.8	6.566	445.6	1.8	0.0786453
16	6.665	455	53.1	0.37182	2	1.53765	5.8	5	6.665	454.2	2	0.435187
17	6.735	462	53.9	0.0899769	4	0.600883	2.3	2	6.735	463.3	3.6	0.0953383
18	6.853	474	55.3	0.0948169	4	0.657323	2.5	2.1	6.853	474.6	4.1	0.0894296
19	6.955	487	56.8	0.0800105	6	0.796783	3	2.6	6.955	486.5	5.7	0.079272
20	7.082	503	58.7	0.235381	4	1.72299	6.5	5.6	7.082	503.4	4.2	0.230104
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.145	511	59.6	0.511111	4	3.24124	12.3	10.6	7.145	511.2	3.5	0.517813
22	7.255	525	61.3	0.0643815	4	0.418667	1.6	1.4	7.255	525.4	3.5	0.0674347
23	8.54	747	87.2	0.0134972	7	0.173172	0.7	0.6	8.54	744.7	6.6	0.0148011
24	8.656	775	90.4	0.0218829	7	0.272458	1	0.9	8.656	773	7.3	0.0211282
25	8.838	790	92.2	0.0136221	8	0.180778	0.7	0.6	8.838	794.2	8.1	0.0125311

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	105	12.2	0.0619989	7	0.714899	9	7.1	4.55	106.3	7.3	0.0552038
2	5.2	206	24	0.124406	4	0.768697	9.7	7.6	5.2	205.9	3.5	0.12278
3	5.85	312	36.3	0.13764	4	1.00286	12.7	9.9	5.85	311.5	4	0.141624
4	6.55	445	51.8	0.107224	6	1.04989	13.2	10.4	6.55	444	5.8	0.102043
5	6.85	475	55.3	0.0160819	5	0.151341	1.9	1.5	6.85	474.7	5.4	0.0157597
6	7.35	539	62.7	0.0589104	4	0.463199	5.8	4.6	7.35	538.9	4.4	0.0592042
7	8.15	659	76.7	0.0943444	6	1.00369	12.7	9.9	8.15	659.1	6.2	0.0916865
8	8.45	722	84.1	0.0912487	6	0.923952	11.7	9.1	8.45	722.1	5.7	0.0920456
9	8.65	774	90.1	0.0679847	4	0.447554	5.6	4.4	8.65	774.1	3.7	0.0687244
10	9.3	829	96.5	0.158276	5	1.39783	17.6	13.8	9.3	828.8	5	0.157573

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.139	47	5.5	0.160138	8	2.11759	7.6	6.6	4.139	48.3	7.6	0.157223
2	4.55	109	12.7	0.0454857	6	0.474519	1.7	1.5	4.55	109.6	6.5	0.0412128
3	4.696	131	15.3	0.0138896	3	0.0441074	0.2	0.1	4.696	130.9	2.5	0.0098584
4	4.795	146	17	0.198038	6	2.08589	7.5	6.5	4.795	147.2	6.3	0.18591
5	4.955	170	19.8	0.15501	10	2.56629	9.2	8	4.955	167.1	9.7	0.148569
6	5.2	207	24.1	0.0491927	5	0.385201	1.4	1.2	5.2	206	4.7	0.0462318
7	5.352	232	27	0.0242103	5	0.199704	0.7	0.6	5.352	230.9	4.7	0.0241029
8	5.419	243	28.3	0.0747306	3	0.401452	1.4	1.3	5.419	243.5	3.1	0.0726803
9	5.637	279	32.5	0.0345428	7	0.404101	1.5	1.3	5.637	278.8	7.3	0.0313285
10	5.85	314	36.6	0.0921212	5	0.742256	2.7	2.3	5.85	314.7	4.7	0.0887102
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.973	337	39.3	0.496974	3	2.86725	10.3	9	5.973	337.3	3.3	0.487891
12	6.059	353	41.1	0.237417	4	1.58523	5.7	5	6.059	352.8	3.9	0.231115
13	6.145	369	43	0.117204	3	0.33237	1.2	1	6.145	367.8	3.3	0.0574469
14	6.182	376	43.8	0.125985	8	1.5263	5.5	4.8	6.182	373.7	8.4	0.102495
15	6.498	435	50.7	0.104598	6	0.995389	3.6	3.1	6.498	435.1	5.5	0.101214
16	6.583	448	52.2	0.0493497	1	0.101982	0.4	0.3	6.583	448	1.4	0.0404483
17	6.671	457	53.3	0.392159	2	1.62764	5.9	5.1	6.671	456.5	2.3	0.402435
18	6.749	465	54.2	0.110361	4	0.816761	2.9	2.6	6.749	465.1	4	0.114772
19	6.855	476	55.5	0.117567	6	1.06466	3.8	3.3	6.855	476.7	5.6	0.107395
20	6.963	490	57.1	0.0763127	5	0.684609	2.5	2.1	6.963	489.1	5.3	0.0730469
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.072	504	58.7	0.140054	4	1.55871	5.6	4.9	7.072	506.7	4.3	0.206784
22	7.149	514	59.9	0.499312	3	3.02992	10.9	9.5	7.149	514.3	3.4	0.503356
23	7.265	529	61.7	0.0458489	3	0.268344	1	0.8	7.265	528.6	3.2	0.0476477
24	7.35	540	62.9	0.0169941	4	0.117648	0.4	0.4	7.35	541	3.6	0.0185236
25	8.15	662	77.2	0.0271719	6	0.321243	1.2	1	8.15	661.5	6.3	0.0288358
26	8.45	724	84.4	0.0274283	5	0.261236	0.9	0.8	8.45	724.3	5.2	0.0284365
27	8.533	744	86.7	0.0208845	6	0.221293	0.8	0.7	8.533	744.4	6.3	0.0199372
28	8.65	772	90	0.0365272	4	0.298417	1.1	0.9	8.65	772.5	4.5	0.0374363
29	8.836	788	91.8	0.0170842	5	0.162047	0.6	0.5	8.836	790.2	5.4	0.0168644
30	9.3	828	96.5	0.0715424	4	0.519704	1.9	1.6	9.3	828	4.1	0.0723623

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.187	51	5.9	0.19689	7	2.43351	8.5	7.4	4.187	52	7.2	0.190021
2	4.579	111	12.9	0.0143842	9	0.204505	0.7	0.6	4.579	113.8	9.4	0.0122844
3	4.736	135	15.7	0.018609	4	0.105511	0.4	0.3	4.736	135.7	3.8	0.0154698
4	4.841	151	17.6	0.283177	6	2.82151	9.8	8.6	4.841	151.1	5.8	0.273254
5	4.997	175	20.4	0.151434	10	2.4601	8.6	7.5	4.997	172.4	9.8	0.142129
6	5.371	234	27.3	0.0209478	6	0.195036	0.7	0.6	5.371	235.9	6.2	0.0178746
7	5.456	248	28.9	0.0737981	4	0.487835	1.7	1.5	5.456	248.2	4.5	0.0616145
8	5.694	287	33.4	0.0147811	18	0.987977	3.4	3	5.694	261.5	18	0.0309676
9	5.863	315	36.7	0.056775	8	0.600548	2.1	1.8	5.863	315.1	7.5	0.0450939
10	5.98	337	39.3	0.574213	3	3.2288	11.3	9.9	5.98	337.3	3.2	0.564444
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.065	353	41.1	0.288631	4	1.84593	6.4	5.6	6.065	352.5	3.8	0.277377
12	6.139	367	42.8	0.134622	4	0.839113	2.9	2.6	6.139	367.2	3.6	0.13064
13	6.187	376	43.8	0.140138	5	1.06153	3.7	3.2	6.187	375.8	5	0.119251
14	6.495	434	50.6	0.12084	5	1.06624	3.7	3.3	6.495	434.6	5	0.119633
15	6.585	448	52.2	0.0564371	2	0.130901	0.5	0.4	6.585	447.3	1.6	0.0461119
16	6.662	456	53.1	0.410295	2	1.6195	5.6	5	6.662	456.4	2.1	0.434364
17	6.748	465	54.2	0.121469	4	0.89938	3.1	2.8	6.748	465.4	4	0.12562
18	6.853	476	55.5	0.122132	5	0.987965	3.4	3	6.853	476.8	4.9	0.11338
19	6.953	489	57	0.0953465	5	0.837673	2.9	2.6	6.953	488.7	5.1	0.0924321
20	7.061	503	58.6	0.147426	4	1.6497	5.7	5	7.061	505.9	4.2	0.221792
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.146	514	59.9	0.518749	3	3.14042	10.9	9.6	7.146	513.6	3.4	0.51979
22	7.254	528	61.5	0.0605941	3	0.378577	1.3	1.2	7.254	528.1	3.3	0.0639057
23	8.545	746	86.9	0.0285807	12	0.521122	1.8	1.6	8.545	744.4	12.3	0.0239236
24	8.65	772	90	0.0109151	4	0.0821145	0.3	0.3	8.65	773.5	4.4	0.0105162
25	8.817	786	91.6	0.0140623	4	0.107957	0.4	0.3	8.817	787.1	4.3	0.0140295

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	106	12.3	0.0496075	7	0.557714	7.7	5.9	4.55	107.2	7	0.0449873
2	5.2	207	24.1	0.099299	4	0.745334	10.3	7.9	5.2	206.8	4.3	0.0981661
3	5.85	313	36.4	0.136643	4	0.980171	13.5	10.3	5.85	313.1	4	0.138594
4	6.55	446	51.9	0.0911784	6	1.0124	14	10.7	6.55	445.6	6.4	0.0893358
5	6.85	478	55.6	0.01736	4	0.124068	1.7	1.3	6.85	478.6	4	0.0175769
6	7.35	543	63.2	0.0592529	5	0.481728	6.6	5.1	7.35	543.3	4.7	0.0578721
7	8.15	663	77.2	0.0716953	7	0.867218	12	9.1	8.15	662.7	6.8	0.0714873
8	8.45	723	84.2	0.0927467	6	0.956489	13.2	10.1	8.45	723	5.9	0.0921025
9	8.65	774	90.1	0.0639284	4	0.440433	6.1	4.6	8.65	774.2	3.8	0.0652112
10	9.3	827	96.3	0.112223	6	1.08185	14.9	11.4	9.3	826.5	5.6	0.108623

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 140

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.900 5.200 8.100 5.850 11.800 6.550 12.500 6.850 2.200 7.350 5.500 8.150 14.300 8.450 12.900 8.650 5.600 9.300 18.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.195 6.500 4.550 1.300 4.694 0.200 4.793 8.900 4.937 4.900 5.200 1.300 5.349 0.500 5.429 1.400 5.646 0.500 5.850 1.800 5.978 11.400 6.060 6.600 6.142 3.100 6.188 2.700 6.485 4.300 6.563 0.700 6.641 6.200 6.729 3.800 6.837 3.200 6.927 3.200 7.079 5.500 7.143 12.600 7.254 0.900 7.350 0.500 8.150 1.600 8.450 1.300 8.542 0.400 8.650 1.500 8.850 1.200 9.300 1.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 30 Return to Lane Navigator
4.206 8.000 4.569 0.800 4.719 0.300 4.810 11.200 4.972 6.800 5.379 0.700 5.453 1.800 5.662 1.100 5.863 1.300 5.982 12.100 6.065 7.100 6.149 3.400 6.190 3.300 6.491 4.800 6.566 0.900 6.665 5.800 6.735 2.300 6.853 2.500 6.955 3.000 7.082 6.500 7.145 12.300 7.255 1.600 8.540 0.700 8.656 1.000 8.838 0.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 25 Return to Lane Navigator
4.550 9.000 5.200 9.700 5.850 12.700 6.550 13.200 6.850 1.900 7.350 5.800 8.150 12.700 8.450 11.700 8.650 5.600 9.300 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.139 7.600 4.550 1.700 4.696 0.200 4.795 7.500 4.955 9.200 5.200 1.400 5.352 0.700 5.419 1.400 5.637 1.500 5.850 2.700 5.973 10.300 6.059 5.700 6.145 1.200 6.182 5.500 6.498 3.600 6.583 0.400 6.671 5.900 6.749 2.900 6.855 3.800 6.963 2.500 7.072 5.600 7.149 10.900 7.265 1.000 7.350 0.400 8.150 1.200 8.450 0.900 8.533 0.800 8.650 1.100 8.836 0.600 9.300 1.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 30 Return to Lane Navigator
4.187 8.500 4.579 0.700 4.736 0.400 4.841 9.800 4.997 8.600 5.371 0.700 5.456 1.700 5.694 3.400 5.863 2.100 5.980 11.300 6.065 6.400 6.139 2.900 6.187 3.700 6.495 3.700 6.585 0.500 6.662 5.600 6.748 3.100 6.853 3.400 6.953 2.900 7.061 5.700 7.146 10.900 7.254 1.300 8.545 1.800 8.650 0.300 8.817 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 25 Return to Lane Navigator
4.550 7.700 5.200 10.300 5.850 13.500 6.550 14.000 6.850 1.700 7.350 6.600 8.150 12.000 8.450 13.200 8.650 6.100 9.300 14.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.