RFE Fish Species Identification Isoelectric Focusing Tools - Yellowtail Snapper PAGPlate IEF Gel 03

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19930812gel05
Unique Web Code: ysg03Srr
Tissue Aliquot Code: ocyuchry 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
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Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	51	4.4	0.218685	12	4.23029	19.2	14.2	3.5	53.3	12	0.198994
2	4.55	190	16.5	0.216493	6	2.2771	10.3	7.6	4.55	189.7	6.4	0.202023
3	5.2	267	23.1	0.253482	5	2.25866	10.2	7.6	5.2	267.3	5.1	0.250077
4	5.85	418	36.2	0.38352	4	2.70934	12.3	9.1	5.85	418.4	4	0.384941
5	6.55	579	50.1	0.187186	6	1.67316	7.6	5.6	6.55	577.6	5.8	0.16315
6	6.85	618	53.5	0.167588	5	1.4791	6.7	5	6.85	617.6	5.3	0.156632
7	7.35	712	61.6	0.207644	6	2.11133	9.6	7.1	7.35	710.9	6.2	0.191391
8	8.15	883	76.5	0.0534917	10	0.889429	4	3	8.15	883	9.5	0.0527177
9	8.45	945	81.8	0.0742928	7	0.870934	3.9	2.9	8.45	945.4	6.6	0.0740558
10	8.65	986	85.4	0.0622162	6	0.631541	2.9	2.1	8.65	986	6	0.0594193
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1122	97.1	0.236209	8	2.95283	13.4	9.9	9.3	1120.5	7.7	0.21559

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	51	4.4	0.216288	12	4.32333	8.8	8.1	3.5	53.3	11.9	0.204887
2	4.007	121	10.5	0.452848	4	3.46673	7.1	6.5	4.007	121.4	4.2	0.465946
3	4.434	180	15.7	0.0237739	8	0.333514	0.7	0.6	4.434	182.5	8.1	0.0233713
4	4.55	196	17.1	0.217742	4	1.49792	3.1	2.8	4.55	195.4	4.5	0.189133
5	4.656	209	18.2	0.0679678	9	1.17434	2.4	2.2	4.656	205.7	9.2	0.0721447
6	4.948	245	21.3	0.0482628	4	0.351171	0.7	0.7	4.948	244.9	4	0.0490559
7	5.094	263	22.9	0.479411	4	3.61896	7.4	6.8	5.094	263.3	4.3	0.479476
8	5.2	276	24	0.189707	4	1.43194	2.9	2.7	5.2	276.5	4.4	0.181936
9	5.292	296	25.8	0.0552584	5	0.3736	0.8	0.7	5.292	295.7	4.7	0.0453268
10	5.342	307	26.7	0.0805782	8	1.01451	2.1	1.9	5.342	308	7.8	0.0734599
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.699	385	33.5	0.120917	9	1.6302	3.3	3	5.699	384.8	8.5	0.107851
12	5.763	399	34.7	0.0469641	6	0.436582	0.9	0.8	5.763	401.8	5.5	0.0447049
13	5.85	418	36.4	0.133989	3	1.039	2.1	1.9	5.85	419.7	3.5	0.167682
14	5.903	430	37.4	0.503119	5	4.83024	9.9	9	5.903	429.8	5.4	0.502586
15	6.012	455	39.6	0.294193	4	2.24066	4.6	4.2	6.012	454.8	4.4	0.288062
16	6.1	475	41.3	0.0924657	6	1.01595	2.1	1.9	6.1	476.4	6.4	0.0894772
17	6.455	556	48.4	0.0429038	6	0.442847	0.9	0.8	6.455	557	6.4	0.0387935
18	6.538	575	50	0.0837728	7	1.01747	2.1	1.9	6.538	571.8	7.1	0.0803878
19	6.614	586	51	0.220014	3	1.23118	2.5	2.3	6.614	587.4	3.1	0.223631
20	6.722	600	52.2	0.032703	9	0.650788	1.3	1.2	6.722	603	8.7	0.0423058
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.829	614	53.4	0.12632	6	1.28027	2.6	2.4	6.829	614.6	6	0.120521
22	6.908	627	54.6	0.036049	6	0.377397	0.8	0.7	6.908	625.7	5.5	0.0386215
23	7.009	645	56.1	0.0243857	6	0.294567	0.6	0.6	7.009	647.1	5.7	0.0291515
24	7.065	655	57	0.0798685	3	0.471146	1	0.9	7.065	655.3	3.4	0.078051
25	7.16	672	58.5	0.402153	4	3.26014	6.7	6.1	7.16	672.4	4.5	0.41258
26	7.26	690	60.1	0.015351	8	0.336022	0.7	0.6	7.26	696	7.6	0.0250821
27	7.35	706	61.4	0.125201	5	1.10708	2.3	2.1	7.35	705.6	5.2	0.120385
28	8.15	879	76.5	0.0243575	17	1.54542	3.2	2.9	8.15	867.8	17.3	0.0503306
29	8.45	933	81.2	0.117154	14	2.62977	5.4	4.9	8.45	934.1	14.1	0.105095
30	8.536	951	82.8	0.0747711	13	1.26373	2.6	2.4	8.536	956.9	12.7	0.0563618
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	975	84.9	0.100433	8	1.3379	2.7	2.5	8.65	974.9	7.7	0.0976225
32	9.3	1112	96.8	0.241189	7	2.84983	5.8	5.3	9.3	1109.8	7.2	0.222198

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.029	120	10.5	0.496315	4	4.00556	9.8	8.9	4.029	119.7	4.5	0.506144
2	4.606	199	17.4	0.0381132	7	0.4897	1.2	1.1	4.606	198.7	7.5	0.0369447
3	4.686	209	18.2	0.0484904	5	0.377558	0.9	0.8	4.686	209.5	4.8	0.0444617
4	4.999	248	21.6	0.0367463	4	0.283772	0.7	0.6	4.999	247.6	4.4	0.0362205
5	5.144	266	23.2	0.432061	5	3.98371	9.7	8.8	5.144	266.2	5.2	0.435411
6	5.32	300	26.2	0.0672928	6	0.743263	1.8	1.6	5.32	301.1	6.2	0.0671914
7	5.422	323	28.2	0.0689577	8	0.913281	2.2	2	5.422	321.4	7.5	0.0684871
8	5.71	388	33.9	0.0777186	7	0.681341	1.7	1.5	5.71	385.1	7	0.0549603
9	5.759	399	34.8	0.101849	10	1.60725	3.9	3.6	5.759	399.4	10	0.0908914
10	5.918	435	38	0.615015	5	5.06139	12.4	11.2	5.918	435.3	4.7	0.602725
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.019	458	40	0.458891	4	3.344	8.2	7.4	6.019	457.3	4.3	0.435988
12	6.084	473	41.3	0.100668	9	2.45374	6	5.4	6.084	477.6	9.4	0.147077
13	6.128	483	42.1	0.104444	1	0.0330451	0.1	0.1	6.128	485.3	0.7	0.0268166
14	6.373	539	47	0.0218002	4	0.165924	0.4	0.4	6.373	538.2	4.1	0.0227744
15	6.448	556	48.5	0.0504751	3	0.263954	0.6	0.6	6.448	556.1	2.9	0.0505396
16	6.522	573	50	0.104501	3	0.555006	1.4	1.2	6.522	573	3.3	0.0954604
17	6.571	582	50.8	0.465925	2	1.93694	4.7	4.3	6.571	582.3	2.3	0.479623
18	6.655	593	51.7	0.0622459	4	0.344346	0.8	0.8	6.655	593.3	3.8	0.0506042
19	6.755	606	52.9	0.148224	7	1.77598	4.3	3.9	6.755	606.9	7	0.144121
20	6.865	621	54.2	0.0610762	4	0.433183	1.1	1	6.865	621.1	4.2	0.0587859
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.959	638	55.7	0.0361569	8	0.64488	1.6	1.4	6.959	642.8	8.5	0.0429567
22	7.02	649	56.6	0.189128	2	0.666906	1.6	1.5	7.02	649.2	2.3	0.164666
23	7.109	665	58	0.651886	3	3.57007	8.7	7.9	7.109	664.8	3.1	0.658345
24	7.209	683	59.6	0.0173176	7	0.20335	0.5	0.5	7.209	686.2	6.6	0.0174732
25	8.492	942	82.2	0.128282	18	4.03851	9.9	9	8.492	945.2	18	0.126585
26	8.606	965	84.2	0.259429	6	2.38488	5.8	5.3	8.606	964.6	6.3	0.212202

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	49	4.3	0.236355	11	4.15673	17.9	13.7	3.5	50.6	10.7	0.218753
2	4.55	194	16.9	0.21301	7	2.57229	11.1	8.5	4.55	193.5	7.3	0.197631
3	5.2	276	24	0.239125	6	2.56521	11	8.5	5.2	275.5	6	0.241663
4	5.85	427	37.2	0.356559	5	3.24041	13.9	10.7	5.85	425.8	5.1	0.35941
5	6.55	587	51.1	0.198588	6	2.08866	9	6.9	6.55	585.2	6.4	0.183222
6	6.85	626	54.5	0.156404	6	1.42981	6.1	4.7	6.85	626.1	5.7	0.140789
7	7.35	717	62.4	0.211664	6	2.09488	9	6.9	7.35	715.8	6.2	0.19121
8	8.15	882	76.8	0.0434853	11	0.795475	3.4	2.6	8.15	881.6	10.8	0.0415909
9	8.45	940	81.8	0.0556804	7	0.673433	2.9	2.2	8.45	939.5	7.1	0.053692
10	8.65	979	85.2	0.0455291	6	0.435876	1.9	1.4	8.65	979.5	5.7	0.0433221
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1110	96.6	0.246207	8	3.21101	13.8	10.6	9.3	1107.7	8.4	0.215662

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	52	4.5	0.196572	14	4.63705	9.7	8.8	3.5	54.5	14.5	0.180734
2	4.001	115	10	0.420186	4	3.10201	6.5	5.9	4.001	115.4	4.1	0.43169
3	4.447	171	14.9	0.0222852	9	0.328852	0.7	0.6	4.447	172.5	8.6	0.0216723
4	4.55	184	16.1	0.210984	4	1.17399	2.5	2.2	4.55	183.9	4	0.167229
5	4.66	197	17.2	0.064575	10	1.27579	2.7	2.4	4.66	192.6	10.1	0.0714267
6	4.972	234	20.4	0.035329	5	0.317446	0.7	0.6	4.972	232	5	0.0360446
7	5.107	250	21.8	0.430646	4	3.08289	6.4	5.9	5.107	249.8	3.9	0.441091
8	5.2	261	22.8	0.166543	6	1.49773	3.1	2.8	5.2	261.5	5.5	0.153497
9	5.287	282	24.6	0.0547623	8	0.649486	1.4	1.2	5.287	282.1	8	0.0457739
10	5.333	293	25.6	0.0846482	5	0.67858	1.4	1.3	5.333	295	4.9	0.0785492
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.679	376	32.8	0.149258	7	1.613	3.4	3.1	5.679	376	6.8	0.133379
12	5.75	393	34.3	0.0301614	8	0.428594	0.9	0.8	5.75	393.3	7.7	0.0314844
13	5.85	417	36.4	0.212898	3	1.04428	2.2	2	5.85	413.8	2.8	0.210444
14	5.892	427	37.3	0.453205	7	6.34476	13.3	12.1	5.892	425.3	7.1	0.506246
15	6.003	453	39.5	0.295593	5	2.54265	5.3	4.8	6.003	452.2	5.2	0.277518
16	6.092	474	41.4	0.0946796	4	0.691492	1.4	1.3	6.092	473.9	4.2	0.0929307
17	6.122	481	42	0.0565314	3	0.283146	0.6	0.5	6.122	482	3	0.0539732
18	6.457	560	48.9	0.0800978	10	1.21257	2.5	2.3	6.457	560.1	9.7	0.0706285
19	6.52	575	50.2	0.110593	7	1.36079	2.8	2.6	6.52	575	7.3	0.105201
20	6.619	591	51.6	0.410496	2	1.45725	3	2.8	6.619	590.8	2	0.41346
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.688	600	52.4	0.0443984	5	0.365339	0.8	0.7	6.688	601.1	5.1	0.0406042
22	6.827	618	53.9	0.139186	8	1.78288	3.7	3.4	6.827	618.8	7.9	0.128103
23	7.064	660	57.6	0.105186	3	0.568547	1.2	1.1	7.064	659.2	3.5	0.0921197
24	7.152	676	59	0.576777	3	3.17489	6.6	6	7.152	676.2	3.3	0.540194
25	7.251	694	60.6	0.0419936	18	0.702276	1.5	1.3	7.251	696.3	18	0.0220124
26	7.35	712	62.1	0.175239	4	1.23217	2.6	2.3	7.35	711.4	4.3	0.160618
27	8.15	879	76.7	0.0328159	17	1.54304	3.2	2.9	8.15	869.9	16.9	0.0516173
28	8.45	936	81.7	0.0709698	12	1.35491	2.8	2.6	8.45	938.4	11.6	0.0657322
29	8.65	980	85.5	0.038553	5	0.321648	0.7	0.6	8.65	980.4	4.9	0.0368862
30	9.3	1115	97.3	0.255191	7	3.09407	6.5	5.9	9.3	1112.7	7.4	0.234462

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.047	129	11.2	0.399681	5	3.26802	9.6	8.8	4.047	128.9	4.6	0.402784
2	4.678	208	18.1	0.0205232	11	0.424395	1.2	1.1	4.678	211.3	10.8	0.0221806
3	4.778	220	19.1	0.0192961	4	0.0527403	0.2	0.1	4.778	220.5	3.9	0.00764854
4	5.043	252	21.9	0.00295933	4	0.0279564	0.1	0.1	5.043	252	4	0.00394322
5	5.217	275	23.9	0.274323	9	4.24302	12.5	11.4	5.217	272.3	9.1	0.263961
6	5.309	297	25.8	0.0358636	8	0.401215	1.2	1.1	5.309	296.9	7.6	0.0298747
7	5.368	311	27	0.0761257	7	0.944191	2.8	2.5	5.368	310.2	7.2	0.0736622
8	5.419	323	28	0.0500573	7	0.623709	1.8	1.7	5.419	322.9	7.3	0.0484297
9	5.717	394	34.2	0.102648	11	1.97016	5.8	5.3	5.717	394.6	11.2	0.0992545
10	5.781	409	35.5	0.0578566	5	0.442624	1.3	1.2	5.781	411.9	5.5	0.0455194
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.905	438	38	0.593131	5	5.35935	15.8	14.4	5.905	438.2	5.3	0.575822
12	6.005	461	40	0.394934	5	3.24569	9.6	8.7	6.005	461	4.9	0.370779
13	6.088	480	41.7	0.155221	8	1.84796	5.4	5	6.088	480.5	7.9	0.131257
14	6.428	558	48.4	0.0387449	3	0.208086	0.6	0.6	6.428	557.7	3	0.0392525
15	6.506	576	50	0.0580002	3	0.266312	0.8	0.7	6.506	575.7	2.7	0.0557633
16	6.55	586	50.9	0.2953	3	1.43556	4.2	3.9	6.55	585.9	2.6	0.313343
17	6.627	596	51.7	0.0385769	7	0.494822	1.5	1.3	6.627	598.7	6.9	0.0402698
18	6.765	614	53.3	0.121213	7	1.34406	4	3.6	6.765	613.6	6.8	0.111948
19	6.942	642	55.7	0.0317355	7	0.367725	1.1	1	6.942	645	6.6	0.0316034
20	7.001	653	56.7	0.108821	3	0.478474	1.4	1.3	7.001	653.1	2.6	0.10267
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.082	668	58	0.485159	4	3.32315	9.8	9	7.082	668.6	3.8	0.491684
22	7.191	688	59.7	0.0139741	4	0.0795262	0.2	0.2	7.191	688.3	3.6	0.012399
23	8.474	948	82.3	0.0852479	17	2.47694	7.3	6.7	8.474	948.2	17.2	0.0810314
24	8.564	967	83.9	0.0885203	6	0.638051	1.9	1.7	8.564	965.9	5.9	0.0611818

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	53	4.6	0.154011	15	3.95847	22.7	18	3.5	55.8	15	0.148911
2	4.55	186	16.3	0.124125	8	1.71376	9.8	7.8	4.55	186	8.1	0.119654
3	5.2	266	23.3	0.138409	7	1.8071	10.3	8.2	5.2	265	7	0.146265
4	5.85	419	36.7	0.276098	5	2.34401	13.4	10.7	5.85	418.3	4.7	0.284156
5	6.55	575	50.3	0.154659	6	1.4993	8.6	6.8	6.55	573.4	6.5	0.130493
6	6.85	614	53.7	0.120833	5	1.06025	6.1	4.8	6.85	613.9	5.5	0.109046
7	7.35	708	61.9	0.158408	6	1.45337	8.3	6.6	7.35	706.7	5.6	0.145925
8	8.15	879	76.9	0.0373325	10	0.639578	3.7	2.9	8.15	876.8	10.4	0.0345412
9	8.45	935	81.8	0.0581312	7	0.672648	3.9	3.1	8.45	935.6	6.7	0.0564287
10	8.65	975	85.3	0.0370471	6	0.37484	2.1	1.7	8.65	974.2	5.8	0.0367546
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1106	96.8	0.178075	7	1.93826	11.1	8.8	9.3	1104.2	6.9	0.157421

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 145

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 19.200 4.550 10.300 5.200 10.200 5.850 12.300 6.550 7.600 6.850 6.700 7.350 9.600 8.150 4.000 8.450 3.900 8.650 2.900 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator
3.500 8.800 4.007 7.100 4.434 0.700 4.550 3.100 4.656 2.400 4.948 0.700 5.094 7.400 5.200 2.900 5.292 0.800 5.342 2.100 5.699 3.300 5.763 0.900 5.850 2.100 5.903 9.900 6.012 4.600 6.100 2.100 6.455 0.900 6.538 2.100 6.614 2.500 6.722 1.300 6.829 2.600 6.908 0.800 7.009 0.600 7.065 1.000 7.160 6.700 7.260 0.700 7.350 2.300 8.150 3.200 8.450 5.400 8.536 2.600 8.650 2.700 9.300 5.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 8 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 10 PeakCount: 32 Return to Lane Navigator
4.029 9.800 4.606 1.200 4.686 0.900 4.999 0.700 5.144 9.700 5.320 1.800 5.422 2.200 5.710 1.700 5.759 3.900 5.918 12.400 6.019 8.200 6.084 6.000 6.128 0.100 6.373 0.400 6.448 0.600 6.522 1.400 6.571 4.700 6.655 0.800 6.755 4.300 6.865 1.100 6.959 1.600 7.020 1.600 7.109 8.700 7.209 0.500 8.492 9.900 8.606 5.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 9 LANE TYPE: Specimen ACTUAL GEL LANE: 11 PeakCount: 26 Return to Lane Navigator
3.500 17.900 4.550 11.100 5.200 11.000 5.850 13.900 6.550 9.000 6.850 6.100 7.350 9.000 8.150 3.400 8.450 2.900 8.650 1.900 9.300 13.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 11 Return to Lane Navigator
3.500 9.700 4.001 6.500 4.447 0.700 4.550 2.500 4.660 2.700 4.972 0.700 5.107 6.400 5.200 3.100 5.287 1.400 5.333 1.400 5.679 3.400 5.750 0.900 5.850 2.200 5.892 13.300 6.003 5.300 6.092 1.400 6.122 0.600 6.457 2.500 6.520 2.800 6.619 3.000 6.688 0.800 6.827 3.700 7.064 1.200 7.152 6.600 7.251 1.500 7.350 2.600 8.150 3.200 8.450 2.800 8.650 0.700 9.300 6.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 11 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 13 PeakCount: 30 Return to Lane Navigator
4.047 9.600 4.678 1.200 4.778 0.200 5.043 0.100 5.217 12.500 5.309 1.200 5.368 2.800 5.419 1.800 5.717 5.800 5.781 1.300 5.905 15.800 6.005 9.600 6.088 5.400 6.428 0.600 6.506 0.800 6.550 4.200 6.627 1.500 6.765 4.000 6.942 1.100 7.001 1.400 7.082 9.800 7.191 0.200 8.474 7.300 8.564 1.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 24 Return to Lane Navigator
3.500 22.700 4.550 9.800 5.200 10.300 5.850 13.400 6.550 8.600 6.850 6.100 7.350 8.300 8.150 3.700 8.450 3.900 8.650 2.100 9.300 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.