Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940810gel01
Unique Web Code: ysg02Srr
Tissue Aliquot Code: ocyuchry 002 #3 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5635.30.322605126.398481612.73.564.2120.300383
24.5521618.30.179602103.164757.96.34.5521510.40.17126
35.229825.30.2693794.0463610.185.2297.98.60.265165
45.8544938.10.59581933.514858.875.85448.83.30.598187
56.5561752.30.28938162.8025275.56.55616.15.90.267796
66.8565655.60.0933015111.6041343.26.85657.610.60.085195
77.3575463.90.47980554.37152118.77.35753.15.30.46391
88.15920780.15861272.024715.148.15919.47.50.153185
98.45979830.31050263.28328.26.58.45978.66.10.30373
108.65102186.50.26658562.597376.55.18.651019.85.60.260338
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.31145970.66236566.07215.2129.311446.10.562855
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Specimen
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.04314412.20.32478652.820828.36.74.043144.350.32031
24.61122418.90.050038870.6064931.81.44.611223.67.20.0473447
34.67523219.60.048465650.3762991.10.94.675234.44.90.0431406
45.14329024.50.61677555.386615.912.85.143290.34.90.623824
55.348331280.0727403101.209043.62.95.348330.410.10.0675864
65.46635830.20.13841740.8611592.525.466358.83.80.128349
75.78543136.40.065295280.68762521.65.785428.47.80.0495208
85.8544637.70.103247111.822795.44.35.85445.810.70.0962522
95.89145638.50.091045360.5352141.61.35.891457.26.20.0490333
106.00348340.80.70951945.016414.811.96.003482.64.10.692146
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.08650342.50.53151143.8049911.29.16.0865034.40.488146
126.17352444.30.040105350.35078510.86.173525.34.80.0410622
136.21953545.20.028007910.04769110.10.16.219534.81.10.0246424
146.35956948.10.1578151.3728443.36.359569.54.80.160891
156.59562152.40.059489760.5093281.51.26.595621.26.20.0460843
166.65562953.10.30332120.963652.82.36.655628.91.90.284799
176.69363453.50.2063131.070353.22.56.693634.42.90.209176
186.82765255.10.039992230.2059670.60.56.827652.530.0391694
196.87666055.70.065003840.4159121.216.876660.63.50.066159
206.94767456.90.037241520.128850.40.36.947673.32.50.0294653
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.0870059.10.1362351.0297232.47.08699.14.90.118741
227.15671560.40.63587333.6343410.78.67.156715.33.20.634207
238.51199884.30.022548100.3574941.10.98.511997.39.80.0205063
248.61101986.10.0327403130.6988292.11.78.611019.912.70.0309456
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: pI Markers
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5655.50.278346115.0977412.89.93.56711.10.259813
24.5521217.90.180899113.268258.26.34.55212.510.70.172198
35.229124.60.27693984.0875510.37.95.2290.88.50.271528
45.8543837.10.47818443.505128.86.85.85438.34.10.481975
56.5560851.40.22128793.214388.16.26.55606.98.80.205297
66.8564954.90.0563799111.040422.626.85649.911.10.053104
77.3574763.20.33430974.3014510.88.37.35746.27.40.330045
88.1592378.10.14456792.378864.68.15921.69.30.143703
98.4598683.40.25454183.5700996.98.45985.680.252591
108.65102987.10.19203293.108437.868.651028.19.10.193561
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3115597.70.82021756.3054115.812.29.31155.14.50.782908
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5756.40.193522144.615357.36.43.576.2140.18636
23.97714011.90.29970952.474093.93.43.977140.34.70.29726
34.5521818.50.161342113.009114.74.24.55217.3110.154531
44.65723019.50.080571340.2513480.40.34.657230.53.60.0393102
55.09327923.60.5144253.892696.15.45.093278.94.50.486695
65.229124.60.16512192.622084.13.65.22908.90.166416
75.30531426.60.0519540.3384160.50.55.305314.740.0478229
85.3532427.40.11007150.9377371.51.35.35324.15.10.104176
95.65939233.20.0840655241.58172.52.25.659397.823.50.0379708
105.705402340.13656791.567892.52.25.705400.49.20.0957892
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.8543436.70.32747931.570422.52.25.85434.32.60.334923
125.89544537.70.64272356.015269.58.35.895445.95.30.641905
136.00847340.10.45447642.908034.646.008473.13.90.418995
146.08949341.70.11336230.66110310.96.089493.33.30.111569
156.12550242.50.03395130.2615930.40.46.125500.22.70.0550291
166.30754746.30.1669330.9765531.51.46.307546.93.20.171028
176.485591500.141235143.16254.46.485587.914.40.123644
186.53602510.25584921.112751.81.56.53603.31.90.332867
196.54660651.30.23308920.6909381.116.546606.72.20.180244
206.72463153.40.096653881.398482.21.96.724632.38.10.0975439
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.02367757.30.063690930.3214550.50.47.023677.730.0613855
227.1269358.70.25799231.524252.42.17.126933.30.260664
237.21170859.90.41847342.908984.647.211708.43.80.429135
247.3573161.90.27987652.578664.13.67.35730.65.20.278671
258.15909770.17822992.760514.33.88.15908.98.90.174377
268.4597782.70.30964363.083314.94.38.45977.15.70.305413
278.65102286.50.31349973.622045.758.651021.36.80.298724
289.3115497.70.77341356.6716710.59.39.31154.350.758494
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Specimen
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0114312.10.32171452.853116.45.14.01143.55.10.318655
24.61922218.80.057738790.88839521.64.619222.69.20.0543096
34.71423319.80.063371350.45706210.84.714233.85.20.0497056
45.1728624.30.5349165.3365912.19.65.17286.25.60.533397
55.34732327.40.078554160.7690571.71.45.3473225.90.073821
65.38733228.20.15903851.3117132.45.387332.750.14814
75.71240634.50.10408650.5211221.20.95.712404.14.80.0607735
85.75141535.20.12988992.125384.83.85.751415.89.40.127198
95.88644637.90.055800910.06910110.20.15.886444.60.70.0576844
105.93145738.80.66035766.5601914.811.85.931457.85.80.64303
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.04948541.20.43694543.0889675.56.049484.54.30.409889
126.12850442.80.13237340.9730642.21.76.128504.44.20.129238
136.16151243.50.077149530.4099810.90.76.161510.92.70.0855241
146.36256047.50.25965431.529483.52.76.362560.23.30.260414
156.52960050.90.099305481.217392.72.26.5295988.40.0817419
166.58661051.80.35293421.376523.12.56.586610.12.20.34616
176.67262252.80.23490731.187842.72.16.672621.630.219838
186.81664254.50.0884322121.708023.93.16.816636.412.20.0790233
197.02967957.60.1572751.166072.62.17.029678.34.70.13976
207.118695590.42286732.168424.93.97.118695.22.90.415078
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.184707600.48245543.018456.85.47.184707.23.60.472307
228.44897682.90.126308183.449517.86.28.448978.8180.108123
238.587100685.40.148901112.08714.73.78.5871007.310.90.107622
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5867.30.252114188.5112620.816.93.588.518.30.262684
24.5521918.50.163559112.942727.25.94.5522110.70.155368
35.229725.10.2491483.596378.87.25.2296.68.20.247505
45.85450380.57383433.508578.675.85450.73.40.58587
56.5561251.70.21963393.142287.76.36.55609.38.80.200751
66.8565455.20.104558101.704684.23.46.85655.310.10.0952349
77.3575263.50.38567853.602928.87.27.35751.25.40.373541
88.1592678.20.14059781.930864.73.88.15925.780.135447
98.4598583.20.27595363.013037.368.45984.76.40.266332
108.65102686.70.21060272.503896.158.651025.470.202902
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3115397.40.64134766.5558616139.31152.46.30.588592
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 108
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 3
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Specimen
ACTUAL GEL LANE: 4
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: pI Markers
ACTUAL GEL LANE: 5
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 6
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Specimen
ACTUAL GEL LANE: 7
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln