Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowfin Tuna  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Thunnus albacares
FDA Seafood List Common Name: Yellowfin Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970130gel01
Unique Web Code: ynh01Srr
Tissue Aliquot Code: thunalba I96-002 #09m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55273.50.1454240.9378975.94.44.5527.73.70.143744
25.2124160.22863841.504739.47.15.2124.43.80.223858
35.8521928.30.21069941.414858.96.75.85218.740.199443
46.5534744.80.22721572.5310215.9126.553466.80.209325
56.8537648.50.11737250.9039835.74.36.85376.24.60.110323
67.3544557.40.18591151.495889.47.17.35444.24.70.179398
78.1556773.20.17885972.2577614.210.78.155677.30.17382
88.4563982.50.14613982.0217812.79.68.45637.98.20.138656
98.6568988.90.13238251.071386.75.18.65688.44.60.131655
109.376198.20.20680261.801511.38.59.3759.45.50.183279
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.639415.30.025229340.1828810.50.44.63940.54.30.0239787
24.717526.70.017024930.09359750.20.24.71751.83.30.016061
35.12210914.10.045829270.5066771.315.122107.86.60.0430748
45.36114418.60.054066360.454211.10.95.3611435.50.0464663
55.54817222.30.05252530.2359390.60.55.548172.52.50.0528434
65.8321427.70.05192430.3163690.80.65.83214.83.50.0512503
76.015247320.031182120.1129330.30.26.01524720.0313096
86.17427635.70.038360720.1277080.30.26.174275.91.90.0377668
96.30630038.80.0208705110.3325210.80.66.306296.6110.0171207
106.44832642.20.038770160.3181910.80.66.448324.85.50.0325452
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.50333643.50.027340920.0598060.10.16.503335.81.60.0215261
126.59334945.10.48617353.735699.37.26.593349.94.60.457207
136.726363470.38439432.113855.34.16.726363.33.30.359774
146.81237248.10.41676732.087125.246.812372.32.90.401982
156.89538249.40.019392710.05140820.10.16.895382.41.50.0198565
167.01439851.50.18873631.088662.72.17.014397.83.40.181579
177.12641353.40.0477829130.9762432.41.97.126421130.0422296
187.25343055.60.56067733.047827.65.87.253430.33.40.508716
197.3243956.80.22760172.63796.65.17.32441.26.60.226256
207.46146059.50.30050431.475933.72.87.461460.52.80.300283
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.61148362.50.51525133.011677.55.87.611482.73.20.532974
227.69649664.20.016224720.07390830.20.17.696495.12.50.0170123
238.37561879.90.118740.80637721.58.375617.93.90.116297
248.50665084.10.53317944.1168710.37.98.5066504.20.549271
258.69169289.50.274196188.4902621.216.38.691693.4180.266122
268.96472293.40.14229191.514573.82.98.964723.99.50.0902193
279.10973895.50.05768140.2969340.70.69.1097393.80.0437168
289.24575397.40.25520741.871744.73.69.245753.14.20.248506
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55273.50.10453340.7759416.95.34.5526.94.20.105258
25.211314.70.13291341.024519.27.15.2112.24.40.132273
35.8521227.60.09945750.9279288.36.45.85212.25.10.101985
46.5533944.10.16142871.8380816.512.76.55337.670.147965
56.8537348.50.058234640.44498843.16.85373.24.40.0567099
67.35438570.076946950.6287275.64.37.35436.84.70.0749887
78.15561730.12976561.477613.210.28.15561.26.50.129135
88.4562981.80.13490281.816116.312.58.45628.87.80.13191
98.6568388.80.10127750.8796317.96.18.65682.350.099749
109.3754980.1467261.3570412.19.49.3752.35.70.134833
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55293.80.070801240.526811.51.14.5529.84.40.0668009
25.2115150.0770350.6291431.81.45.21144.70.0750501
35.37114118.30.017021650.1420690.40.35.371141.25.10.0156223
45.56117022.10.029364370.32983810.75.561169.86.90.0271461
55.8521427.80.11610140.7853872.31.75.85214.340.111821
66.03224732.10.015118440.09111980.30.26.032247.23.50.0145546
76.44732241.90.043908630.246050.70.56.447321.53.40.0405877
86.51933543.60.028462520.1179710.30.36.519334.72.50.0271658
96.60534745.10.43799342.591347.65.76.605347.43.90.379662
106.72836146.90.37606332.029164.46.728361.73.40.335945
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.80637048.10.41396931.90945.64.26.806370.32.70.402251
127.01139651.50.12230730.67657621.57.011395.43.10.124588
137.2442655.40.11158240.7708362.31.77.24424.64.40.0991709
147.30943556.60.50724653.9149711.58.57.309434.54.80.464118
157.46445859.60.26030831.346483.92.97.46445830.25676
167.59447862.20.47019632.410437.15.37.594478.32.80.481207
178.37661680.10.039801760.4751431.418.376616.86.20.0431499
188.4563382.30.025418520.1032410.30.28.45633.12.40.0238513
198.50264784.10.50559632.936238.66.48.502646.63.30.506928
208.6568789.30.244332187.407321.716.28.65688.8180.232178
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.94371893.40.150278122.366696.95.28.943719.812.10.110688
229.08573395.30.079482940.4079551.20.99.085735.34.40.0517778
239.24675097.50.22747351.876455.54.19.246750.64.90.217325
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.622374.80.036157650.2376730.60.64.6223750.0269674
24.666435.60.031430580.4133771.114.66646.78.30.0282104
35.077100130.0354245100.5313111.41.35.07797.49.70.0309275
45.37114218.50.024490150.2088470.50.55.371143.45.30.0222618
55.56917122.30.025270160.2572610.70.65.569173.360.0242553
65.85521327.80.032988580.38990410.95.8552158.30.0264877
76.03124531.90.021373950.181990.50.46.031243.35.50.0186729
86.17427135.30.026219730.1208770.30.36.174271.62.60.0259993
96.43331841.50.039430740.2346070.60.66.433317.33.50.0376631
106.50433143.20.027687930.1319230.30.36.504330.12.70.0280201
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.5934444.90.40446553.1081187.36.59344.94.70.370071
126.7235946.80.3302342.214775.75.26.72358.83.90.321604
136.80736948.10.36794531.762284.54.26.807368.62.80.356041
147.00239451.40.14532751.159332.77.002394.550.13098
157.25442755.70.23458592.299465.95.47.254423.79.40.138146
167.30743456.60.28656383.9244810.19.27.307436.17.90.281637
177.46245759.60.2382841.529013.93.67.462457.83.90.224026
187.61648162.70.41510832.610646.76.27.616480.83.50.424698
198.14456373.40.13611630.7094921.81.78.1445633.20.123719
208.37361880.60.10526640.7633421.88.373618.14.20.103761
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.50264984.60.51271743.496898.28.502648.73.80.523006
228.69268989.80.240355197.4609719.217.68.692689.218.60.226208
238.96271893.60.145024163.430338.88.18.962717.116.50.117406
249.11173495.70.046421320.02043890.109.111734.41.80.0065827
259.2574997.70.27622341.735924.54.19.25748.83.90.248465
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55283.70.16050841.0646.14.84.5528.43.80.156874
25.212215.90.26867541.8133110.38.25.2121.740.25823
35.8521327.80.16444661.5837797.15.85213.65.50.161919
46.5534144.50.24685262.6435315.111.96.55340.26.40.232114
56.85376490.084174460.884736546.85376.26.10.0815001
67.3544257.60.16822751.5852997.17.35441.85.50.163315
78.1556773.90.160084102.8220816.112.78.1556810.20.156125
88.4564383.80.132333102.2053112.69.98.45640.6100.124877
98.6568589.30.10608571.325047.668.65686.47.30.102493
109.375698.60.15485671.599599.17.29.3753.36.50.138786
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 106
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln