RFE Fish Species Identification Isoelectric Focusing Tools - Yellowtail Rockfish PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes flavidus
FDA Seafood List Common Name: Yellowtail Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940913gel04
Unique Web Code: yeg01Srr
Tissue Aliquot Code: sebaflav I94-004 #07a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Specimen	Lane e Co-Migrated	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	46	4	0.178418	20	5.86572	22.7	18.6	3.5	41.9	20.2	0.164121
2	4.55	202	17.5	0.312666	4	2.02064	7.8	6.4	4.55	201.6	4.1	0.280686
3	5.2	282	24.4	0.360334	4	2.48741	9.6	7.9	5.2	281.5	4	0.3508
4	5.85	422	36.5	0.448055	3	2.29851	8.9	7.3	5.85	422.3	2.9	0.451903
5	6.55	594	51.3	0.321375	4	2.25482	8.7	7.2	6.55	594.3	4	0.315343
6	6.85	631	54.5	0.119767	6	1.17804	4.6	3.7	6.85	631.5	6.5	0.102352
7	7.35	723	62.5	0.267563	5	2.10683	8.2	6.7	7.35	722.5	4.5	0.263245
8	8.15	879	76	0.0967185	9	1.57243	6.1	5	8.15	878.6	9.4	0.0946592
9	8.45	954	82.5	0.146914	6	1.61363	6.3	5.1	8.45	954	6.4	0.142514
10	8.65	1001	86.5	0.168042	5	1.38993	5.4	4.4	8.65	1000.5	4.8	0.162403
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1143	98.8	0.531575	3	3.01625	11.7	9.6	9.3	1142.4	3.2	0.524255

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.181	145	12.6	0.391864	4	2.95259	16.9	9	4.181	145.2	4.1	0.405243
2	4.731	222	19.2	0.479931	4	3.595	20.6	10.9	4.731	222.2	4.1	0.494751
3	5.888	432	37.4	0.429941	3	2.51047	14.4	7.6	5.888	433.2	3.5	0.4066
4	5.951	448	38.8	0.291635	3	1.42257	8.1	4.3	5.951	448.1	2.8	0.290924
5	5.994	459	39.7	0.234096	4	1.45762	8.3	4.4	5.994	459.5	3.7	0.22222
6	6.046	472	40.9	0.0487598	3	0.270446	1.5	0.8	6.046	471.5	3.3	0.0458615
7	6.105	487	42.2	0.0215781	2	0.197708	1.1	0.6	6.105	484.3	2	0.0551842
8	6.125	492	42.6	0.0185005	1	0	0	0	6.125	489	1	0
9	6.16	501	43.4	0.395887	2	1.12677	6.4	3.4	6.16	500.8	1.6	0.405897
10	6.378	556	48.1	0.067275	7	0.750622	4.3	2.3	6.378	556.1	7.4	0.0573236
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.449	574	49.7	0.0464645	2	0.16275	0.9	0.5	6.449	573.8	2.1	0.0427432
12	6.493	585	50.6	0.0765732	6	0.748478	4.3	2.3	6.493	584.3	5.6	0.075814
13	6.54	597	51.7	0.172463	6	1.53185	8.8	4.7	6.54	597.4	5.8	0.14829
14	7.652	790	68.4	0.125835	3	0.761771	4.4	2.3	7.652	789.7	3.4	0.1251

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.7	0.192975	19	5.94801	20.7	16.3	3.5	38.8	18.8	0.178744
2	4.55	197	17.1	0.33407	4	2.29002	8	6.3	4.55	196.8	4.3	0.299058
3	5.2	279	24.2	0.379725	4	2.68457	9.3	7.4	5.2	279	4.1	0.366337
4	5.85	423	36.7	0.446256	3	2.72994	9.5	7.5	5.85	423.3	3.4	0.456014
5	6.55	605	52.4	0.32957	4	2.48619	8.6	6.8	6.55	604.5	4.3	0.323443
6	6.85	642	55.6	0.127794	6	1.18643	4.1	3.3	6.85	642.2	6	0.1109
7	7.35	737	63.9	0.298928	5	2.52325	8.8	6.9	7.35	736	4.9	0.291708
8	8.15	899	77.9	0.103753	10	1.69468	5.9	4.7	8.15	898.4	9.7	0.0983087
9	8.45	966	83.7	0.144533	7	1.76707	6.1	4.9	8.45	965.7	6.9	0.144297
10	8.65	1008	87.3	0.162103	5	1.45785	5.1	4	8.65	1007.6	5.3	0.15484
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1137	98.5	0.457132	5	3.97785	13.8	10.9	9.3	1137.3	4.8	0.464439

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.109	134	11.6	0.408596	4	2.97981	16.2	10	4.109	134.9	4	0.418862
2	4.621	207	18	0.439368	4	3.25419	17.7	11	4.621	207.4	4.1	0.452501
3	5.868	427	37	0.46625	4	2.84827	15.5	9.6	5.868	427.4	3.7	0.43162
4	5.946	447	38.8	0.297371	3	1.58612	8.6	5.3	5.946	446.5	2.9	0.310473
5	5.977	455	39.5	0.26504	3	1.51249	8.2	5.1	5.977	455.3	3.3	0.255244
6	6.024	467	40.5	0.035806	5	0.298164	1.6	1	6.024	468.5	4.6	0.0366347
7	6.087	483	41.9	0.0798156	2	0.259712	1.4	0.9	6.087	483.5	1.7	0.0844158
8	6.114	490	42.5	0.0403562	2	0.121112	0.7	0.4	6.114	490.5	1.6	0.0428487
9	6.157	501	43.5	0.471973	1	1.24155	6.8	4.2	6.157	500.5	1.3	0.535565
10	6.38	558	48.4	0.0671583	6	0.742546	4	2.5	6.38	557	6	0.0694544
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.446	575	49.9	0.0620346	2	0.235899	1.3	0.8	6.446	575.2	2.3	0.0583012
12	6.489	586	50.8	0.0913235	5	0.825307	4.5	2.8	6.489	585.5	5.4	0.0870055
13	6.54	599	52	0.180716	5	1.48661	8.1	5	6.54	598.5	5.4	0.153947
14	7.648	792	68.7	0.136774	4	0.953576	5.2	3.2	7.648	792.2	4	0.135495

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.2	0.170742	11	3.27818	9	7	3.5	46.7	10.8	0.171446
2	4.126	138	12	0.331624	5	2.81943	7.7	6	4.126	139.2	4.9	0.323838
3	4.55	199	17.3	0.117833	5	0.942623	2.6	2	4.55	197.6	4.8	0.11143
4	4.652	212	18.4	0.320688	5	2.78033	7.6	5.9	4.652	212.5	5	0.315133
5	5.2	282	24.5	0.17316	7	2.055	5.6	4.4	5.2	280	7.2	0.160437
6	5.85	422	36.6	0.178403	2	0.627336	1.7	1.3	5.85	421.5	2.1	0.168642
7	5.878	429	37.2	0.354757	4	2.57405	7	5.5	5.878	429.2	4.4	0.32717
8	5.949	447	38.8	0.220424	4	1.59687	4.4	3.4	5.949	447.7	3.8	0.238216
9	5.989	457	39.7	0.225168	4	1.41187	3.9	3	5.989	457.4	3.7	0.215844
10	6.045	471	40.9	0.0405591	4	0.287812	0.8	0.6	6.045	471.5	4.3	0.0377828
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.105	486	42.2	0.0571545	2	0.209754	0.6	0.4	6.105	486.6	1.9	0.0637167
12	6.136	494	42.9	0.0347165	3	0.1987	0.5	0.4	6.136	494.9	3.5	0.0321151
13	6.172	503	43.7	0.452417	2	1.83007	5	3.9	6.172	503.2	2.2	0.469367
14	6.407	562	48.8	0.0327468	4	0.290226	0.8	0.6	6.407	560.4	3.8	0.0433677
15	6.474	579	50.3	0.0308603	2	0.0911985	0.2	0.2	6.474	579.1	1.6	0.0313933
16	6.518	590	51.2	0.0786314	4	0.633307	1.7	1.3	6.518	590.2	4.4	0.0810596
17	6.55	598	51.9	0.104663	3	0.487276	1.3	1	6.55	597.3	2.9	0.0950839
18	6.588	603	52.3	0.129111	3	0.657405	1.8	1.4	6.588	603	3.2	0.115835
19	6.85	638	55.4	0.0841522	5	0.649584	1.8	1.4	6.85	637.3	4.7	0.0774323
20	7.35	728	63.2	0.259491	4	2.00934	5.5	4.3	7.35	727.8	4.3	0.261195
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.717	800	69.4	0.108572	5	0.954929	2.6	2	7.717	800.6	5.1	0.105933
22	8.15	885	76.8	0.41578	4	2.67913	7.3	5.7	8.15	885.9	3.6	0.418578
23	8.45	951	82.6	0.0801433	10	1.26934	3.5	2.7	8.45	949.7	9.6	0.074749
24	8.65	996	86.5	0.107453	5	0.976008	2.7	2.1	8.65	995.9	5.2	0.105641
25	9.3	1116	96.9	0.244901	12	5.25866	14.4	11.2	9.3	1115.7	12	0.247447

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.7	0.206716	9	3.27842	12.6	9.6	3.5	44.9	9.4	0.196028
2	4.55	191	16.6	0.234874	7	2.1834	8.4	6.4	4.55	191.9	6.6	0.186789
3	5.2	273	23.7	0.330162	5	2.61453	10	7.7	5.2	273.2	4.6	0.321111
4	5.85	419	36.4	0.441487	3	2.69244	10.3	7.9	5.85	418.5	3.4	0.446654
5	6.55	596	51.8	0.333508	4	2.31561	8.9	6.8	6.55	596.2	4	0.325322
6	6.85	633	55	0.139434	6	1.31449	5	3.9	6.85	632.5	6.2	0.119134
7	7.35	724	63	0.310275	5	2.4909	9.6	7.3	7.35	722.8	4.7	0.300474
8	8.15	880	76.5	0.0910423	10	1.57731	6.1	4.6	8.15	879.8	10.1	0.0877655
9	8.45	948	82.4	0.128984	7	1.46356	5.6	4.3	8.45	948	6.5	0.12659
10	8.65	989	86	0.132884	6	1.36432	5.2	4	8.65	989.5	5.9	0.129806
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1100	95.7	0.196031	15	4.76405	18.3	14	9.3	1098	14.6	0.184703

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK SP CO MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 22.700 4.550 7.800 5.200 9.600 5.850 8.900 6.550 8.700 6.850 4.600 7.350 8.200 8.150 6.100 8.450 6.300 8.650 5.400 9.300 11.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
4.181 16.900 4.731 20.600 5.888 14.400 5.951 8.100 5.994 8.300 6.046 1.500 6.105 1.100 6.125 0 6.160 6.400 6.378 4.300 6.449 0.900 6.493 4.300 6.540 8.800 7.652 4.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 14 Return to Lane Navigator
3.500 20.700 4.550 8.000 5.200 9.300 5.850 9.500 6.550 8.600 6.850 4.100 7.350 8.800 8.150 5.900 8.450 6.100 8.650 5.100 9.300 13.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
4.109 16.200 4.621 17.700 5.868 15.500 5.946 8.600 5.977 8.200 6.024 1.600 6.087 1.400 6.114 0.700 6.157 6.800 6.380 4.000 6.446 1.300 6.489 4.500 6.540 8.100 7.648 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 14 Return to Lane Navigator
3.500 9.000 4.126 7.700 4.550 2.600 4.652 7.600 5.200 5.600 5.850 1.700 5.878 7.000 5.949 4.400 5.989 3.900 6.045 0.800 6.105 0.600 6.136 0.500 6.172 5.000 6.407 0.800 6.474 0.200 6.518 1.700 6.550 1.300 6.588 1.800 6.850 1.800 7.350 5.500 7.717 2.600 8.150 7.300 8.450 3.500 8.650 2.700 9.300 14.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 25 Return to Lane Navigator
3.500 12.600 4.550 8.400 5.200 10.000 5.850 10.300 6.550 8.900 6.850 5.000 7.350 9.600 8.150 6.100 8.450 5.600 8.650 5.200 9.300 18.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.