RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Wolffish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Anarhichas lupus
FDA Seafood List Common Name: Atlantic Wolffish
FDA Seafood List Market Name: Wolffish
Unique IEF Gel Code: 19970212gel01
Unique Web Code: wfh02Srr
Tissue Aliquot Code: anarlupu H94-002 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	27	3.5	0.0373097	5	0.2994	4.9	3.8	4.55	26.8	4.6	0.0366033
2	5.2	115	15	0.0589242	6	0.53836	8.8	6.8	5.2	114.7	5.5	0.0551793
3	5.85	235	30.6	0.114801	4	0.853668	13.9	10.8	5.85	234.8	4.3	0.112461
4	6.55	368	48	0.0760826	10	1.26538	20.7	16	6.55	365.2	9.9	0.0717524
5	6.85	405	52.8	0.0315856	4	0.230416	3.8	2.9	6.85	405.5	4.2	0.0311054
6	7.35	472	61.5	0.0442424	4	0.334374	5.5	4.2	7.35	471.6	4.3	0.0442005
7	8.15	588	76.7	0.0808738	7	0.990525	16.2	12.5	8.15	586.6	7.1	0.0782849
8	8.45	649	84.6	0.0797615	7	0.928847	15.2	11.8	8.45	648.5	6.7	0.0785158
9	8.65	700	91.3	0.0561661	4	0.433031	7.1	5.5	8.65	700.1	4.5	0.0548225
10	9.3	755	98.4	0.0441852	3	0.249983	4.1	3.2	9.3	754.4	3	0.0467911

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.585	30	3.9	0.0083819	5	0.0679161	0.2	0.1	4.585	31.3	5.4	0.00708101
2	4.732	51	6.6	0.00702722	2	0.0257823	0.1	0	4.732	52	1.5	0.00945799
3	4.823	64	8.3	0.161854	5	1.38446	3.3	2.6	4.823	64	4.9	0.159779
4	4.899	75	9.8	0.166242	6	1.7375	4.1	3.3	4.899	74.8	6	0.164053
5	5.13	108	14.1	0.110788	8	1.53012	3.6	2.9	5.13	105.1	8.3	0.10352
6	5.232	124	16.2	0.116686	11	2.3162	5.4	4.4	5.232	123.5	11.4	0.114901
7	5.561	185	24.1	0.0245097	2	0.10178	0.2	0.2	5.561	184.6	2.3	0.0251895
8	5.642	200	26.1	0.0954068	3	0.554879	1.3	1.1	5.642	200.2	3.5	0.0903792
9	5.756	221	28.8	0.139315	3	0.745611	1.8	1.4	5.756	221.4	2.9	0.146422
10	5.815	232	30.2	0.0775414	3	0.480111	1.1	0.9	5.815	232.1	3.4	0.0802502
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.902	248	32.3	0.278353	6	2.83048	6.6	5.4	5.902	247.8	5.7	0.278841
12	5.945	256	33.4	0.171791	4	0.979535	2.3	1.9	5.945	256.3	4	0.138023
13	6.01	268	34.9	0.393155	5	3.27034	7.7	6.3	6.01	267.7	4.6	0.398573
14	6.303	322	42	0.123809	8	1.57943	3.7	3	6.303	322.6	8	0.111206
15	6.336	328	42.8	0.536291	3	2.18956	5.1	4.2	6.336	328.4	2.6	0.480458
16	6.57	370	48.2	0.140176	9	2.22087	5.2	4.2	6.57	368.9	9.4	0.133882
17	6.684	384	50.1	0.313738	3	1.61432	3.8	3.1	6.684	383.3	3	0.304684
18	6.789	397	51.8	0.447711	3	2.57564	6	4.9	6.789	397.5	3.2	0.449395
19	7.566	500	65.2	0.159506	17	4.68483	11	9	7.566	497.4	16.7	0.158094
20	8.783	710	92.6	0.368269	19	11.698	27.5	22.4	8.783	707.6	18.8	0.351618

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	23	3	0.0498956	5	0.399342	4.9	3.7	4.55	22.8	4.7	0.0483289
2	5.2	121	15.8	0.069123	7	0.801892	9.7	7.4	5.2	119.5	7.1	0.0636786
3	5.85	242	31.6	0.13715	4	1.08121	13.1	10	5.85	241.5	4.4	0.137107
4	6.55	367	47.8	0.0990348	8	1.29158	15.7	12	6.55	365.6	8.1	0.0899523
5	6.85	404	52.7	0.0456778	5	0.39772	4.8	3.7	6.85	403.7	5.2	0.0434173
6	7.35	464	60.5	0.0739832	4	0.567947	6.9	5.3	7.35	464.2	4.5	0.0712637
7	8.15	585	76.3	0.114399	7	1.37297	16.7	12.7	8.15	585.3	7	0.110804
8	8.45	646	84.2	0.105323	7	1.28047	15.6	11.9	8.45	645.5	7	0.103492
9	8.65	697	90.9	0.0821323	5	0.646307	7.9	6	8.65	697	4.5	0.0802193
10	9.3	754	98.3	0.063175	3	0.390261	4.7	3.6	9.3	753.1	3.4	0.0643259

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	16	2.1	0.0150397	5	0.14202	0.8	0.7	4.55	16.9	5.5	0.0146216
2	4.723	43	5.6	0.00647874	1	0	0	0	4.723	36	1	0
3	4.799	55	7.2	0.0642797	4	0.19563	1.2	1	4.799	55.7	3.5	0.0312093
4	4.857	64	8.4	0.0823988	10	1.40786	8.3	7.1	4.857	64.2	9.7	0.0815911
5	5.023	90	11.7	0.0881668	7	1.00327	5.9	5.1	5.023	92	6.8	0.0827831
6	5.145	109	14.2	0.0482421	12	0.952707	5.6	4.8	5.145	107.5	12	0.0449334
7	5.768	221	28.9	0.0328433	6	0.303735	1.8	1.5	5.768	222.2	5.6	0.0307954
8	5.85	236	30.8	0.0810578	5	0.630164	3.7	3.2	5.85	235.3	4.6	0.0780165
9	5.904	246	32.1	0.0479931	4	0.34867	2.1	1.8	5.904	245.9	4.2	0.046469
10	5.957	256	33.4	0.0730367	2	0.290039	1.7	1.5	5.957	256.6	2.2	0.0739165
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.011	266	34.7	0.379707	3	1.81291	10.7	9.1	6.011	265.6	2.7	0.385099
12	6.28	316	41.3	0.0555764	6	0.505503	3	2.6	6.28	315.8	5.6	0.0512302
13	6.312	322	42	0.295612	2	1.14199	6.7	5.8	6.312	321.8	2.3	0.284081
14	6.5	357	46.6	0.0757316	9	1.14368	6.7	5.8	6.5	356.9	9	0.0713032
15	6.615	373	48.7	0.0991093	2	0.392612	2.3	2	6.615	372.9	2.4	0.0933581
16	6.742	386	50.4	0.274517	3	1.17519	6.9	5.9	6.742	386.2	2.5	0.260922
17	6.85	397	51.8	0.0248418	4	0.149455	0.9	0.8	6.85	397.1	3.7	0.0227776
18	7.35	459	59.9	0.026342	4	0.193857	1.1	1	7.35	458.2	4.3	0.0252514
19	7.568	492	64.2	0.0673837	9	1.09722	6.5	5.5	7.568	493.1	9.2	0.067303
20	8.15	580	75.7	0.0354225	9	0.43197	2.5	2.2	8.15	579	9.1	0.026877
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	647	84.5	0.0331341	6	0.329545	1.9	1.7	8.45	646.7	5.6	0.0333585
22	8.65	697	91	0.145327	14	3.16469	18.7	16	8.65	696.9	13.6	0.131454
23	9.3	752	98.2	0.022799	3	0.130931	0.8	0.7	9.3	752.2	3.2	0.0231164

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.793	53	6.9	0.040373	8	0.6115	1.4	1.1	4.793	55.6	8.5	0.0405942
2	4.881	66	8.6	0.18452	4	0.704189	1.6	1.3	4.881	63.7	4.1	0.0970047
3	4.941	75	9.8	0.214024	9	3.40219	7.8	6.2	4.941	74	9.3	0.206591
4	5.151	106	13.9	0.129434	15	3.22074	7.4	5.9	5.151	105.6	14.9	0.122291
5	5.248	122	15.9	0.120122	10	1.64016	3.8	3	5.248	125.4	10.5	0.0884623
6	5.607	187	24.4	0.00641163	9	0.0581766	0.1	0.1	5.607	186.7	8.7	0.0037933
7	5.695	203	26.5	0.0320179	6	0.317964	0.7	0.6	5.695	201.1	5.9	0.0301938
8	5.8	222	29	0.110501	4	0.74746	1.7	1.4	5.8	221.8	4.1	0.103341
9	5.844	230	30.1	0.0575862	2	0.252431	0.6	0.5	5.844	230.1	2.5	0.0575058
10	5.939	248	32.4	0.219764	6	2.55831	5.9	4.7	5.939	246.6	6.1	0.235356
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.981	256	33.5	0.137294	2	0.492218	1.1	0.9	5.981	255.5	2.3	0.121154
12	6.028	265	34.6	0.492721	3	2.75853	6.3	5.1	6.028	264.9	3.2	0.493236
13	6.285	314	41	0.132379	8	1.38154	3.2	2.5	6.285	314.5	7.7	0.10119
14	6.321	321	42	0.564849	2	2.34126	5.4	4.3	6.321	321.2	2.5	0.531318
15	6.4	336	43.9	0.0167368	2	0.0436943	0.1	0.1	6.4	335.7	1.7	0.0144732
16	6.505	356	46.5	0.136467	5	0.796576	1.8	1.5	6.505	355.6	4.8	0.0945266
17	6.531	361	47.2	0.167748	7	1.70048	3.9	3.1	6.531	362.9	6.6	0.146468
18	6.66	376	49.2	0.342599	4	2.05867	4.7	3.8	6.66	375.3	3.6	0.322552
19	6.787	389	50.8	0.48731	3	2.61127	6	4.8	6.787	388.9	3.2	0.463675
20	7.56	489	63.9	0.169164	15	4.31077	9.9	7.9	7.56	489	15.2	0.159496
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.708	700	91.5	0.349999	20	11.4741	26.4	21	8.708	696.8	19.6	0.331057

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.041532	4	0.309261	4.7	3.3	4.55	19.3	4.3	0.0403939
2	5.2	110	14.4	0.0584253	6	0.592751	9.1	6.4	5.2	111.1	5.9	0.0570022
3	5.85	227	29.7	0.149978	3	0.922006	14.1	10	5.85	226.7	3.4	0.150817
4	6.55	364	47.6	0.108486	6	1.09455	16.7	11.8	6.55	363.7	6.2	0.100251
5	6.85	395	51.6	0.0390946	5	0.311418	4.8	3.4	6.85	395.8	4.7	0.0375334
6	7.35	457	59.7	0.059229	4	0.401367	6.1	4.3	7.35	457	3.8	0.0593892
7	8.15	576	75.3	0.0801956	6	0.828436	12.7	8.9	8.15	576.3	6	0.0777607
8	8.45	641	83.8	0.0805676	6	0.839494	12.8	9.1	8.45	640.5	5.9	0.0800089
9	8.65	694	90.7	0.0681013	4	0.448642	6.9	4.8	8.65	693.4	3.7	0.0678579
10	9.3	751	98.2	0.0905212	5	0.796496	12.2	8.6	9.3	750	5.3	0.0840252

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.900 5.200 8.800 5.850 13.900 6.550 20.700 6.850 3.800 7.350 5.500 8.150 16.200 8.450 15.200 8.650 7.100 9.300 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.585 0.200 4.732 0.100 4.823 3.300 4.899 4.100 5.130 3.600 5.232 5.400 5.561 0.200 5.642 1.300 5.756 1.800 5.815 1.100 5.902 6.600 5.945 2.300 6.010 7.700 6.303 3.700 6.336 5.100 6.570 5.200 6.684 3.800 6.789 6.000 7.566 11.000 8.783 27.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 4.900 5.200 9.700 5.850 13.100 6.550 15.700 6.850 4.800 7.350 6.900 8.150 16.700 8.450 15.600 8.650 7.900 9.300 4.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 0.800 4.723 0 4.799 1.200 4.857 8.300 5.023 5.900 5.145 5.600 5.768 1.800 5.850 3.700 5.904 2.100 5.957 1.700 6.011 10.700 6.280 3.000 6.312 6.700 6.500 6.700 6.615 2.300 6.742 6.900 6.850 0.900 7.350 1.100 7.568 6.500 8.150 2.500 8.450 1.900 8.650 18.700 9.300 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
4.793 1.400 4.881 1.600 4.941 7.800 5.151 7.400 5.248 3.800 5.607 0.100 5.695 0.700 5.800 1.700 5.844 0.600 5.939 5.900 5.981 1.100 6.028 6.300 6.285 3.200 6.321 5.400 6.400 0.100 6.505 1.800 6.531 3.900 6.660 4.700 6.787 6.000 7.560 9.900 8.708 26.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 21 Return to Lane Navigator
4.550 4.700 5.200 9.100 5.850 14.100 6.550 16.700 6.850 4.800 7.350 6.100 8.150 12.700 8.450 12.800 8.650 6.900 9.300 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Wolffish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Wolffish ---- Go to Display Gel Lane Navigator.