RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Wolffish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Anarhichas lupus
FDA Seafood List Common Name: Atlantic Wolffish
FDA Seafood List Market Name: Wolffish
Unique IEF Gel Code: 19950831gel13
Unique Web Code: wfh01Srr
Tissue Aliquot Code: anarlupu H94-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	40	5.3	0.0221108	7	0.247785	9.8	7.3	4.55	39.7	6.6	0.0211345
2	5.2	135	17.9	0.0160768	7	0.183724	7.3	5.4	5.2	135.7	6.6	0.0157006
3	5.85	242	32.1	0.0157991	8	0.206938	8.2	6.1	5.85	242.3	8.1	0.0144283
4	6.55	364	48.3	0.0288114	11	0.555221	21.9	16.3	6.55	361.6	11.4	0.027367
5	8.15	578	76.8	0.0359081	9	0.505686	20	14.8	8.15	577.2	8.9	0.0320849
6	8.45	644	85.5	0.0344354	8	0.497713	19.7	14.6	8.45	642.5	8.3	0.0338058
7	8.65	690	91.6	0.0283321	7	0.333539	13.2	9.8	8.65	689.8	6.7	0.0279647

PeakCounter: 7

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.9	88	11.7	0.188726	8	2.69363	13.2	10.3	4.9	87	8.1	0.187407
2	5.109	119	15.8	0.0693616	17	2.05785	10.1	7.9	5.109	123.9	17.3	0.0671704
3	5.662	210	27.8	0.0172168	3	0.0828599	0.4	0.3	5.662	209.9	2.7	0.0174112
4	5.775	229	30.3	0.0528034	5	0.464056	2.3	1.8	5.775	229	5	0.0526656
5	5.905	251	33.2	0.104708	11	1.94122	9.5	7.4	5.905	253.7	10.6	0.103289
6	6.01	269	35.6	0.310101	4	2.13157	10.4	8.2	6.01	269.2	4.1	0.294851
7	6.174	297	39.3	0.00899181	13	0.806381	3.9	3.1	6.174	317.9	13.1	0.0347457
8	6.337	325	43	0.371835	3	1.77622	8.7	6.8	6.337	324.9	2.9	0.346389
9	6.506	354	46.9	0.0417455	8	0.504441	2.5	1.9	6.506	356	8.3	0.0343079
10	6.629	372	49.3	0.0600349	3	0.261063	1.3	1	6.629	371.4	2.6	0.0566855
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.711	383	50.7	0.18251	4	1.2496	6.1	4.8	6.711	383.4	3.8	0.186183
12	7.406	476	63	0.0576415	13	1.2827	6.3	4.9	7.406	479.1	13.2	0.0548511
13	8.658	690	91.4	0.17212	18	5.22298	25.5	20	8.658	688.8	17.9	0.164239

PeakCounter: 13

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.2	0.0191988	7	0.22971	10.9	7.4	4.55	32.5	7.1	0.0182253
2	5.2	130	17.2	0.0117002	10	0.220236	10.4	7.1	5.2	128.9	10.3	0.0120579
3	5.85	241	31.9	0.0121486	14	0.241805	11.5	7.8	5.85	241.8	13.5	0.0100716
4	6.55	359	47.5	0.0218675	10	0.36711	17.4	11.9	6.55	357.9	10.3	0.0200641
5	8.15	573	75.8	0.0268573	8	0.369266	17.5	11.9	8.15	572.8	8	0.0259526
6	8.45	636	84.1	0.0241927	8	0.358744	17	11.6	8.45	634.8	8.5	0.0238407
7	8.65	686	90.7	0.0302136	7	0.324921	15.4	10.5	8.65	684.9	6.6	0.0279817

PeakCounter: 7

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	36	4.7	0.00987107	8	0.133003	1.4	1.1	4.55	38.1	8.4	0.00890834
2	4.94	91	12	0.073547	8	1.03547	10.7	8.6	4.94	90.6	8.1	0.0719083
3	5.089	112	14.8	0.00651436	9	0.0990514	1	0.8	5.089	111.2	8.9	0.00630899
4	5.271	140	18.5	0.00771244	7	0.0931001	1	0.8	5.271	138.2	7.4	0.00710821
5	5.666	209	27.6	0.0073868	4	0.0531269	0.6	0.4	5.666	208.4	4.1	0.0073711
6	5.781	229	30.2	0.0216657	5	0.15251	1.6	1.3	5.781	228	5.1	0.0167365
7	5.85	241	31.8	0.0316603	3	0.0457412	0.5	0.4	5.85	238.3	3.4	0.00767573
8	5.918	252	33.2	0.0524712	15	1.3077	13.6	10.9	5.918	252.9	14.7	0.0502819
9	6.029	270	35.6	0.148858	4	0.964094	10	8	6.029	269.9	4	0.135621
10	6.35	322	42.5	0.158811	3	0.90143	9.3	7.5	6.35	321.8	3.3	0.154568
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.517	349	46	0.0310196	5	0.069575	0.7	0.6	6.517	347.5	5.1	0.00764723
12	6.555	355	46.8	0.0339154	10	0.559224	5.8	4.7	6.555	354.8	9.6	0.0327504
13	6.651	368	48.5	0.0384037	3	0.186912	1.9	1.6	6.651	367.3	3.2	0.0332824
14	6.725	378	49.9	0.107595	5	0.850685	8.8	7.1	6.725	378.3	4.7	0.102875
15	7.477	480	63.3	0.0239101	11	0.43933	4.6	3.7	7.477	484	11.1	0.0222478
16	8.45	638	84.2	0.0241733	24	0.794611	8.2	6.6	8.45	653.2	24	0.01868
17	8.65	686	90.5	0.0882525	14	1.96072	20.3	16.3	8.65	686.6	14.3	0.0775603

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.937	88	11.6	0.145482	8	1.95181	12.2	9.7	4.937	87.7	7.6	0.145489
2	5.12	114	15	0.0105398	4	0.0711911	0.4	0.4	5.12	115.1	3.8	0.0105162
3	5.245	133	17.5	0.00959336	9	0.140738	0.9	0.7	5.245	134.8	9.4	0.00843707
4	5.646	202	26.6	0.0123631	3	0.0652216	0.4	0.3	5.646	202.9	3.1	0.0119811
5	5.763	222	29.2	0.0364709	6	0.336412	2.1	1.7	5.763	223.1	5.7	0.0332124
6	5.906	246	32.4	0.111434	10	1.98039	12.4	9.9	5.906	247.3	10.3	0.10796
7	6.012	263	34.7	0.262826	4	1.86971	11.7	9.3	6.012	263.2	4.2	0.249841
8	6.353	318	41.9	0.296168	3	1.70893	10.7	8.5	6.353	317.4	3.3	0.291347
9	6.527	346	45.6	0.0311748	4	0.174801	1.1	0.9	6.527	344.6	4.3	0.0228513
10	6.582	354	46.6	0.0219076	8	0.3065	1.9	1.5	6.582	353.7	8.2	0.0211288
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.662	365	48.1	0.0597035	2	0.244818	1.5	1.2	6.662	364.3	2.5	0.0557399
12	6.742	376	49.5	0.16277	4	1.05887	6.6	5.3	6.742	376.4	3.7	0.161618
13	7.483	478	63	0.0530353	13	1.11662	7	5.6	7.483	478.6	12.7	0.0496186
14	8.683	690	90.9	0.163789	18	4.92857	30.9	24.6	8.683	688.7	17.7	0.157299

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	30	3.9	0.0166871	7	0.196181	7.9	5.6	4.55	31.7	7	0.0158237
2	5.2	123	16.2	0.010943	10	0.171779	6.9	4.9	5.2	120.3	9.5	0.0101842
3	5.85	233	30.6	0.0097931	8	0.138729	5.6	4	5.85	232.1	8.4	0.00931007
4	6.55	345	45.3	0.0193079	16	0.510271	20.5	14.6	6.55	339.6	16	0.0180082
5	8.15	568	74.6	0.0358283	7	0.654907	26.3	18.8	8.15	565.8	7.1	0.0518193
6	8.45	630	82.8	0.0324119	9	0.474317	19.1	13.6	8.45	628.9	9.1	0.0295518
7	8.65	678	89.1	0.0274613	8	0.341742	13.7	9.8	8.65	678.3	7.6	0.0254913

PeakCounter: 7

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 65

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.800 5.200 7.300 5.850 8.200 6.550 21.900 8.150 20.000 8.450 19.700 8.650 13.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 7 Return to Lane Navigator
4.900 13.200 5.109 10.100 5.662 0.400 5.775 2.300 5.905 9.500 6.010 10.400 6.174 3.900 6.337 8.700 6.506 2.500 6.629 1.300 6.711 6.100 7.406 6.300 8.658 25.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 13 Return to Lane Navigator
4.550 10.900 5.200 10.400 5.850 11.500 6.550 17.400 8.150 17.500 8.450 17.000 8.650 15.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 7 Return to Lane Navigator
4.550 1.400 4.940 10.700 5.089 1.000 5.271 1.000 5.666 0.600 5.781 1.600 5.850 0.500 5.918 13.600 6.029 10.000 6.350 9.300 6.517 0.700 6.555 5.800 6.651 1.900 6.725 8.800 7.477 4.600 8.450 8.200 8.650 20.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 17 Return to Lane Navigator
4.937 12.200 5.120 0.400 5.245 0.900 5.646 0.400 5.763 2.100 5.906 12.400 6.012 11.700 6.353 10.700 6.527 1.100 6.582 1.900 6.662 1.500 6.742 6.600 7.483 7.000 8.683 30.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 14 Return to Lane Navigator
4.550 7.900 5.200 6.900 5.850 5.600 6.550 20.500 8.150 26.300 8.450 19.100 8.650 13.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 7 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Wolffish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Wolffish ---- Go to Display Gel Lane Navigator.