Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Mozambique Tilapia  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Oreochromis mossambicus
FDA Seafood List Common Name: Mozambique Tilapia
FDA Seafood List Market Name: Tilapia
Unique IEF Gel Code: 19971029gel02
Unique Web Code: tmh01Srr
Tissue Aliquot Code: oreomoss K96-001 #03m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5510011.50.057414350.451155.64.54.55100.34.60.0553255
25.219021.80.065463460.6234017.76.25.2190.75.70.0613117
35.8530134.60.10254250.90560111.395.85301.45.10.101175
46.5544050.50.11867281.6792520.916.66.55438.68.30.114813
56.8547754.80.028226280.39914546.85477.68.20.0273742
67.3554862.90.083600850.7757349.67.77.35548.35.30.082732
78.1567277.20.071124270.88353118.78.156727.30.0680815
88.4573884.70.094677471.0914613.610.88.45737.76.60.0926991
98.6578890.50.072339740.527176.65.28.65788.14.30.0699728
109.383896.20.078746350.7083298.879.3837.55.30.0754359
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.061364.10.41486985.8674319.315.54.06137.280.412574
24.83139160.3215163.3083210.98.74.83139.15.80.32441
35.32120924.10.030908660.30884610.85.32121060.0291085
45.44423026.50.12908430.6605422.21.75.444229.72.90.128575
55.50324027.60.4224943.0756210.18.15.503239.54.20.410467
65.68527131.20.32159842.170077.15.75.685270.83.80.323173
75.76228432.70.1014250.8212882.72.25.762282.74.80.0960729
85.84429834.30.32597942.02086.65.35.844297.93.60.316801
95.93131636.40.14424661.3854.63.75.931315.55.70.136417
105.98432737.60.043868940.378991.215.9843264.40.0484595
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.07534639.80.0759240.5079961.71.36.075345.73.90.0741604
126.10335240.50.12085630.5618781.81.56.103351.82.80.113997
136.25138344.10.066877830.3646481.216.251382.73.20.064794
146.33740146.10.0358168110.7339942.41.96.337401.611.40.036392
156.53344250.90.082615560.8200832.72.26.533439.96.10.0759132
166.58645051.80.0578908100.7630572.526.58645310.20.0423802
176.66446052.90.35564831.610115.34.36.664459.82.60.344002
186.75147154.20.084351130.4515981.51.26.751471.83.30.0763354
196.86248555.80.030437850.2253210.70.66.862482.15.30.0239108
206.93149456.80.42097742.801899.27.46.931493.83.80.417283
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.39155563.90.13816640.92311132.47.391554.63.80.136146
227.7861670.90.05406270.6458362.11.77.786166.90.0528868
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5510311.90.051116450.4238295.94.54.55103.24.80.0498639
25.218721.50.045021670.5726386.15.2186.87.40.0435727
35.8529734.20.087029560.94627113.110.15.85296.56.10.0877466
46.5545151.90.10383591.5872522176.55449.790.0995323
56.8549056.40.030542280.3782485.34.16.85491.27.80.027312
67.3554963.20.077056250.7130489.97.67.35549.25.30.0754396
78.1567677.80.055489270.6835259.57.38.156767.40.0522718
88.4573684.70.061157270.78109110.88.48.45736.57.30.060326
98.6578790.60.062955140.4657816.558.65786.94.30.0613169
109.383596.10.070023750.64875979.3835.15.40.0673437
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0113540.40590985.7512118.315.64.01136.480.407374
24.5510311.90.055455550.439841.41.24.551034.60.0544152
34.85114116.30.32333863.2929310.58.94.851140.75.70.326675
45.218521.30.033578540.2430380.80.75.2184.33.90.0348649
55.28199230.028228750.2328390.70.65.28200.24.60.0282766
65.382217250.096337830.4526971.41.25.382217.32.60.0987392
75.43922726.20.41232642.732448.77.45.439226.93.80.401103
85.63226130.10.29886642.20425765.632261.34.30.291739
95.71227531.70.072216360.742812.425.712273.65.70.0739459
105.80329133.60.31417242.142556.85.85.803291.840.304975
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.89430935.60.14486781.743615.54.75.894308.87.50.130614
125.9431936.80.075383930.2915460.90.85.94320.42.90.0573465
136.02333738.90.074835240.493891.61.36.0233373.90.0714798
146.0634539.80.11539130.5574781.81.56.06344.82.80.111401
156.21637943.70.067579330.3319211.10.96.216378.52.70.0681139
166.30339845.90.019841290.31543410.96.303396.69.40.0190207
176.56145352.20.069304280.8384032.72.36.5614527.60.0622229
186.58545652.60.067191330.1072850.30.36.585456.63.20.0187654
196.747054.20.31682321.335224.23.66.7470.22.30.323345
206.77447955.20.0652017100.8764742.82.46.774482.110.30.047906
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.91449757.30.42751742.634978.47.16.914497.23.50.420059
227.3555664.10.12412861.202963.83.37.35554.85.60.121443
237.76662271.70.0447573120.9043322.92.47.766625.412.20.0416549
248.1568378.80.026063180.3510431.10.98.15682.87.60.0260687
258.4574285.60.045819260.479621.51.38.45742.55.90.0458503
268.6579091.10.04601350.400431.31.18.65789.25.10.0440676
279.383696.40.04655640.3563561.119.3835.94.30.0472691
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.028374.30.4386386.2983820.216.24.02837.88.10.440917
24.8414216.40.34610663.5227111.39.14.84141.85.70.347137
35.30520723.90.033258350.2886030.90.75.305207.75.10.0322051
45.40222425.80.12696420.5875341.91.55.402224.52.50.132771
55.459234270.45463142.89919.37.55.4592343.80.433244
65.65326830.90.34590742.281337.35.95.653268.53.80.335388
75.74428432.80.098859450.7803522.525.744282.44.80.0916614
85.82329834.40.32915542.1892775.65.823298.43.80.323494
95.90731536.30.17668961.742735.64.55.907314.95.80.169008
105.95432537.50.058895320.2770510.90.75.954324.52.50.0627781
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.02334039.20.076730540.5025981.61.36.023340.33.80.0750689
126.056347400.11117130.5824171.91.56.056347.42.90.111608
136.23738644.50.055136440.3913981.316.237385.24.10.0534995
146.34440947.20.0306823110.5468571.81.46.344407.7110.0280575
156.54445252.10.113375111.836925.94.76.544453.510.90.0951177
166.66146653.70.40271431.887326.14.96.661466.52.70.391281
176.73947554.80.085389430.4625081.51.26.739474.930.0863454
186.88249256.70.47392632.8202697.36.882492.53.30.47846
197.38656164.70.12776840.8481322.72.27.386560.43.90.123321
207.78762371.90.033722870.4290271.41.17.787623.27.30.0333154
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5511012.60.030488150.275201544.55109.65.10.0305678
25.219622.50.037520860.4005097.35.85.2196.56.10.0372114
35.8531035.60.064551550.58565310.78.55.85310.35.10.064234
46.5545952.80.090724291.3815325.3206.554579.20.0850609
56.8549156.40.015030280.1917963.52.86.85489.98.30.0129788
67.3555964.30.028935270.3376696.24.97.35559.47.30.0262095
78.15679780.054345670.66979312.39.78.15678.77.10.0531258
88.4574085.10.048430990.75049913.710.98.45740.18.70.0487179
98.6578590.20.022128890.3351966.14.98.65787.29.40.0201239
109.3844970.043543580.5320969.77.79.3842.87.60.0395712
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 99
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln