Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Silk Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus vivanus
FDA Seafood List Common Name: Silk Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940818gel01
Unique Web Code: ssg02Srr
Tissue Aliquot Code: lutjviva 003 #2 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5504.40.33966116.1876614.310.63.551.711.10.314986
24.5519216.90.30052363.150367.35.44.551926.40.279126
35.226923.60.41780763.986279.26.85.2268.75.50.406148
45.8542837.60.61754644.7439110.98.15.85428.14.20.632347
56.5559352.10.35469963.4737866.555925.90.333046
66.8563455.70.14936892.088124.83.66.85635.18.80.13391
77.3572663.80.42099664.5232810.47.87.35725.86.30.403241
88.1589078.20.120033102.108364.93.68.15889.710.30.115284
98.4595383.70.23511283.17047.35.48.45953.17.70.231565
108.6599387.30.20384772.516795.84.38.65992.47.20.196121
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3112398.70.61527877.4424217.212.89.31120.97.40.568647
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.779897.80.21215172.698344.23.23.77988.87.10.214185
24.32216414.40.67714666.8388210.68.24.322163.95.50.696461
35.02625322.20.18037971.794142.82.25.026252.36.90.147157
45.06825822.70.1308840.4505930.70.55.068257.84.20.0601453
55.18327223.90.070342540.4501980.70.55.183272.23.80.0669017
65.28729525.90.062293150.5065580.80.65.287293.45.10.0560891
75.34931027.20.39039942.496233.935.349309.840.354198
85.64238133.50.67383256.7387110.48.15.642381.15.50.696531
95.78241536.40.19729362.027983.12.45.782415.36.30.180485
105.86543538.20.1296861.406132.21.75.865435.16.50.122659
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.96545840.20.81332157.1537511.18.65.9654595.20.782994
126.05647942.10.61419555.052057.86.16.056478.74.90.580642
136.12549543.50.21179372.752974.33.36.125494.67.30.213663
146.52458751.50.23569793.1992953.86.524585.28.90.202417
156.64860653.20.60793198.5947713.310.36.648608.48.60.561949
167.28271262.50.12423651.107071.71.37.282712.74.90.126677
177.8181871.80.20644862.157583.32.67.81817.95.90.205528
188.869103590.90.231867135.251818.16.38.869103213.20.224728
199.076107794.60.103643173.8794264.79.0761067.316.90.129897
209.115108595.30.052206330.0169953009.1151085.12.70.00349832
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5514.50.423919128.2972316.312.33.553.111.90.392716
24.5519917.50.41416263.895337.75.84.55199.35.90.374862
35.227924.50.57249155.1982310.27.75.2278.35.20.558747
45.8543538.20.673945.1643810.27.65.85434.54.20.694043
56.5559352.10.39707653.433046.85.16.555925.20.375689
66.8563255.50.1790482.455494.83.66.856338.50.163932
77.3572363.50.47707464.909759.77.37.35722.360.459584
88.1588477.60.138076112.5150153.78.15884.2110.129535
98.4594883.20.29100673.68127.25.48.459487.30.284492
108.6598886.70.28488362.999685.94.48.659886.10.277093
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3112298.50.70520778.2363416.212.29.31120.770.663979
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5524.60.26577693.959644.843.552.98.90.25013
23.735867.60.11250461.288981.51.33.735866.50.11237
34.2616214.20.58403955.497786.65.54.26161.65.20.598361
44.5520417.90.19700782.366322.82.44.55204.67.50.177852
54.91224821.80.15920192.229112.72.24.912249.38.90.141599
64.9725522.40.13428730.1416930.20.14.97256.330.0267591
75.07726823.50.11291350.9301371.10.95.077268.34.80.108324
85.228324.80.34326963.526494.23.65.2282.86.20.321683
95.303307270.36280831.913922.31.95.303307.33.10.346534
105.61838033.40.64816456.055397.36.15.618380.45.10.67131
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.76441436.30.17192361.763772.11.85.764414.96.20.161268
125.8543438.10.48758343.80294.63.85.85434.14.40.490569
135.95645840.20.79759857.045548.57.15.956459.64.90.806571
146.05748142.20.66038144.780375.74.86.057480.44.30.626482
156.13249843.70.18997882.668613.22.76.132497.180.189103
166.53258951.70.30476394.429525.34.56.532586.49.40.266908
176.73961854.30.569552108.9796710.89.16.739614.910.20.496366
187.3571963.10.39243963.928834.747.35718.25.70.38613
197.86481871.80.2103482.729623.32.87.864817.97.90.194191
208.1587376.60.0949063182.877693.52.98.15878.517.90.0908267
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.4594182.60.15328971.993152.428.45941.97.30.153244
228.6598686.60.1846562.025992.428.65985.56.30.182771
238.819102189.60.0803929131.738922.11.88.8191022.412.70.0769551
249.006106093.10.034783660.3161710.40.39.0061059.35.60.0320826
259.045106893.80.024698670.3023890.40.39.0451071.67.20.0236457
269.3112198.40.64412365.994087.269.31119.75.80.586801
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.733867.50.17683482.366513.42.83.73385.97.60.176526
24.27516314.30.66777656.4899.47.74.2751635.30.688516
34.93924921.80.14668461.3578521.64.939249.260.127096
44.98925522.40.13569130.5080350.70.64.989256.32.90.0991836
55.10526923.60.09630440.6414840.90.85.105269.53.90.0940025
65.25329325.70.086918250.69410310.85.253292.350.0777247
75.32230927.10.50967232.42323.52.95.322309.12.90.477481
85.6338133.40.70707856.498549.47.75.63381.64.90.75254
95.77141436.30.23658862.231313.22.65.771414.95.80.218291
105.84843237.90.1762961.77762.62.15.848433.96.20.160796
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.96145840.20.83717456.894359.98.25.961458.94.60.843179
126.053479420.72136245.140667.46.16.053478.64.30.680944
136.12349543.40.24638583.723565.44.46.123495.48.20.256597
146.52458751.50.32993483.983245.74.76.524584.880.28026
156.70661453.90.6958651314.31420.6176.706610.412.90.62739
167.28271062.30.11120240.8399891.217.282710.44.30.110071
177.82481571.50.2088751.924282.82.37.824815.35.30.206139
188.835102589.90.180505154.69116.85.68.835102615.30.172969
199.027106493.30.10967101.729192.529.0271060.69.70.100311
209.081107594.30.0779355101.156171.71.49.0811075.19.80.0663366
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5534.70.469754108.1615116.211.83.55310.30.448002
24.5519917.50.40856964.009367.95.84.55199.360.375943
35.227724.30.59146755.4642210.87.95.2276.55.40.57377
45.8543037.80.65356845.0961210.17.45.85430.34.30.668756
56.55592520.44757853.985857.95.86.55591.15.40.417817
66.8563355.60.16238892.37664.73.46.85632.89.10.147118
77.3572463.60.43712164.732529.46.87.35723.56.40.417341
88.1588477.60.115186112.102494.238.15884.810.70.11057
98.4594783.10.26615383.5309275.18.45947.47.80.256025
108.6598886.70.27169662.959665.94.38.65987.36.40.260864
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3111798.10.74032678.078191611.79.31114.96.60.692732
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 99
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln