RFE Fish Species Identification Isoelectric Focusing Tools - Shortfin Mako Shark Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Shortfin Mako Shark ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Isurus oxyrinchus
FDA Seafood List Common Name: Shortfin Mako Shark
FDA Seafood List Market Name: Shark, Mako
Unique IEF Gel Code: 19970219gel05
Unique Web Code: snh01Srr
Tissue Aliquot Code: isurus I94-001 #03 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	16	2.1	0.0590002	4	0.404898	4	3.1	4.55	16.5	4.1	0.0554502
2	5.2	115	14.9	0.0858493	6	0.813635	8.1	6.3	5.2	115.9	5.9	0.0782051
3	5.85	227	29.5	0.165627	3	0.985573	9.8	7.6	5.85	227.1	3.3	0.16733
4	6.55	372	48.3	0.131997	7	1.54812	15.4	12	6.55	370.3	7	0.12519
5	6.85	402	52.2	0.0487696	5	0.372774	3.7	2.9	6.85	402.2	4.7	0.0443453
6	7.35	468	60.8	0.0741905	4	0.474344	4.7	3.7	7.35	468	3.8	0.0707008
7	8.15	584	75.8	0.0865832	9	1.37615	13.7	10.7	8.15	584.8	9.5	0.0818116
8	8.45	652	84.7	0.115968	8	1.62264	16.2	12.6	8.45	651.1	8.1	0.113034
9	8.65	706	91.7	0.117471	4	0.819814	8.2	6.4	8.65	706.3	4	0.114267
10	9.3	744	96.6	0.108324	9	1.60732	16	12.5	9.3	743.2	9	0.101202

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.796	216	28.1	0.0615495	3	0.328328	1.8	1.7	5.796	215.7	3.1	0.0590981
2	5.99	254	33	0.0526249	5	0.396034	2.1	2	5.99	253.5	4.5	0.0495386
3	6.435	345	44.8	0.0191523	4	0.142394	0.8	0.7	6.435	344.8	4.4	0.0181138
4	6.518	362	47	0.0113033	3	0.061595	0.3	0.3	6.518	362.9	3.1	0.0110374
5	6.61	375	48.7	0.0148647	3	0.0813652	0.4	0.4	6.61	375	2.8	0.0161173
6	6.758	391	50.8	0.130605	5	1.01987	5.5	5.2	6.758	390.5	5	0.115757
7	6.895	407	52.9	0.574291	2	2.3464	12.6	11.9	6.895	406.5	2.5	0.529891
8	6.955	415	53.9	0.0969302	9	1.60979	8.7	8.1	6.955	412.3	9.2	0.0988568
9	7.045	427	55.5	0.192466	3	1.11043	6	5.6	7.045	426.4	3.4	0.18615
10	7.226	451	58.6	0.082052	4	0.518824	2.8	2.6	7.226	451.1	4.1	0.071969
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.346	467	60.6	0.0667059	3	0.175696	0.9	0.9	7.346	466	2.8	0.0355394
12	7.389	473	61.4	0.0774471	8	0.940726	5.1	4.8	7.389	473.1	7.9	0.0674681
13	7.502	489	63.5	0.151408	7	1.79913	9.7	9.1	7.502	487.6	7.5	0.135543
14	7.572	499	64.8	0.0565746	3	0.242798	1.3	1.2	7.572	498.8	3.1	0.0446947
15	7.65	510	66.2	0.0285459	3	0.121709	0.7	0.6	7.65	510.4	3.2	0.021288
16	7.854	539	70	0.177182	14	4.27285	23	21.6	7.854	537.7	14.3	0.16882
17	8.403	637	82.7	0.0308633	6	0.286881	1.5	1.5	8.403	636.4	5.7	0.0285783
18	8.915	719	93.4	0.215877	6	2.31455	12.5	11.7	8.915	717.7	6	0.218694
19	9.206	736	95.6	0.0138556	2	0.0423254	0.2	0.2	9.206	735.9	1.9	0.0123232
20	9.411	748	97.1	0.1279	3	0.740823	4	3.7	9.411	747.4	3.3	0.127237

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	11	1.4	0.037695	5	0.290926	2.7	2	4.55	11.6	4.6	0.0354214
2	5.2	108	14	0.0435067	8	0.578805	5.4	4	5.2	108.2	7.9	0.0412257
3	5.85	224	29.1	0.1669	4	1.14284	10.6	7.9	5.85	223.4	3.8	0.169383
4	6.55	365	47.5	0.145816	8	1.8744	17.4	13	6.55	364.3	7.6	0.139252
5	6.85	400	52	0.0680199	5	0.590757	5.5	4.1	6.85	400	5.5	0.0611473
6	7.35	467	60.7	0.132962	4	0.989389	9.2	6.9	7.35	466.7	4.4	0.12796
7	8.15	578	75.2	0.120824	7	1.41292	13.1	9.8	8.15	577.8	7.1	0.11241
8	8.45	643	83.6	0.128322	8	1.83434	17	12.7	8.45	642.6	8.2	0.126175
9	8.65	701	91.2	0.129452	4	0.932348	8.7	6.5	8.65	700.9	4.2	0.124121
10	9.3	739	96.1	0.14545	4	1.12416	10.4	7.8	9.3	738.8	4.4	0.143733

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.7	0.0311125	6	0.29687	1.3	1.2	4.55	21.5	5.6	0.02991
2	5.2	115	15	0.0410154	7	0.545081	2.4	2.3	5.2	115.3	7.3	0.0418479
3	5.803	217	28.2	0.0411044	4	0.270769	1.2	1.1	5.803	216.3	3.9	0.0391496
4	5.85	225	29.3	0.0935611	4	0.589031	2.6	2.4	5.85	224.8	3.5	0.0939825
5	6.007	256	33.3	0.0325918	5	0.283633	1.3	1.2	6.007	255.8	5.1	0.0314346
6	6.449	343	44.6	0.0283102	9	0.362629	1.6	1.5	6.449	346.5	8.9	0.0229664
7	6.55	363	47.2	0.0593978	5	0.52531	2.3	2.2	6.55	362.6	5.3	0.0563463
8	6.85	393	51.1	0.11339	6	1.08522	4.8	4.5	6.85	393.5	6.2	0.0988377
9	6.96	409	53.2	0.536137	3	2.83511	12.6	11.8	6.96	409.5	3	0.529821
10	7.097	429	55.8	0.158148	4	1.0994	4.9	4.6	7.097	428.2	4.1	0.149996
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.254	452	58.8	0.0468063	4	0.307257	1.4	1.3	7.254	451.5	4.2	0.0409378
12	7.35	466	60.6	0.118323	4	0.824858	3.7	3.4	7.35	466.4	4.3	0.107255
13	7.496	487	63.3	0.120344	11	2.1602	9.6	9	7.496	485.2	11	0.110719
14	7.58	499	64.9	0.0556782	3	0.201864	0.9	0.8	7.58	499	3.4	0.0337263
15	7.663	511	66.4	0.0169235	2	0.0308126	0.1	0.1	7.663	511.8	1.7	0.0103209
16	7.9	545	70.9	0.0850895	13	1.93932	8.6	8.1	7.9	543.7	13.5	0.0810882
17	8.15	581	75.6	0.0425879	6	0.418393	1.9	1.7	8.15	582.1	5.7	0.0413439
18	8.367	631	82.1	0.0117943	4	0.0852168	0.4	0.4	8.367	630.1	4.4	0.0109999
19	8.45	650	84.5	0.0391386	5	0.331178	1.5	1.4	8.45	649.2	4.6	0.0402284
20	8.848	712	92.6	0.286354	8	4.32401	19.1	18	8.848	709.7	8.3	0.292256
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.204	733	95.3	0.181821	10	3.3162	14.7	13.8	9.204	733.3	9.7	0.192718
22	9.357	742	96.5	0.218217	3	0.756094	3.3	3.1	9.357	742.3	3.3	0.130348

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.798	214	27.9	0.0631634	3	0.35538	1.2	1.1	5.798	213.8	3.2	0.0634971
2	6.005	254	33.1	0.059398	4	0.367002	1.2	1.2	6.005	253.8	3.8	0.0540534
3	6.42	337	43.9	0.0176179	5	0.150097	0.5	0.5	6.42	338.4	4.9	0.0172065
4	6.515	356	46.4	0.0129577	4	0.0812558	0.3	0.3	6.515	357	3.7	0.0123872
5	6.633	372	48.4	0.0159722	3	0.0917265	0.3	0.3	6.633	370.8	3.4	0.015053
6	6.79	389	50.7	0.113794	5	0.925924	3.2	3	6.79	388.2	5	0.103742
7	6.92	405	52.7	0.534174	3	2.81725	9.6	9.1	6.92	405.1	3	0.523039
8	7.061	424	55.2	0.192657	4	1.2267	4.2	4	7.061	423.6	3.8	0.180341
9	7.231	447	58.2	0.0679302	4	0.45168	1.5	1.5	7.231	446.2	4	0.0637523
10	7.335	461	60	0.0480321	3	0.272867	0.9	0.9	7.335	461.1	3.2	0.048678
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.377	467	60.8	0.0389418	3	0.184823	0.6	0.6	7.377	467.1	2.7	0.0389486
12	7.437	476	62	0.0534476	3	0.270928	0.9	0.9	7.437	476.2	2.9	0.0518686
13	7.484	483	62.9	0.114938	4	0.692766	2.4	2.2	7.484	483	3.6	0.109165
14	7.572	496	64.6	0.0572438	7	0.743786	2.5	2.4	7.572	493.9	6.9	0.0610432
15	7.666	510	66.4	0.029463	4	0.155026	0.5	0.5	7.666	511.3	3.5	0.0248601
16	7.767	525	68.4	0.067073	5	0.209556	0.7	0.7	7.767	523.7	5.4	0.0217895
17	7.881	542	70.6	0.172419	14	3.9613	13.5	12.8	7.881	540.3	13.7	0.163346
18	8.376	632	82.3	0.0227582	5	0.212737	0.7	0.7	8.376	631.5	5.5	0.0219147
19	8.561	677	88.2	0.0242323	14	1.44944	4.9	4.7	8.561	691.1	14.4	0.0568923
20	8.874	713	92.8	0.403234	10	6.71209	22.8	21.7	8.874	711.9	10.2	0.370315
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.227	734	95.6	0.29458	7	1.83697	6.3	5.9	9.227	728.5	7	0.147837
22	9.378	743	96.7	0.346523	11	6.20596	21.1	20	9.378	741.1	11.3	0.31069

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	17	2.2	0.0516244	4	0.369801	3.2	2.5	4.55	16.8	4.2	0.0494042
2	5.2	108	14.1	0.0601996	7	0.654019	5.7	4.4	5.2	108.8	6.5	0.0566355
3	5.85	221	28.8	0.163304	4	1.03275	9	6.9	5.85	221.5	3.6	0.162619
4	6.55	363	47.3	0.134858	8	1.90574	16.5	12.7	6.55	361.6	8.4	0.127791
5	6.85	398	51.8	0.0594552	4	0.447237	3.9	3	6.85	398.6	4.4	0.057479
6	7.35	460	59.9	0.129403	4	0.926411	8	6.2	7.35	460	4.1	0.127252
7	8.15	583	75.9	0.139079	7	1.63983	14.2	11	8.15	583.1	7.2	0.128608
8	8.45	647	84.2	0.193694	7	2.18791	19	14.6	8.45	646.6	6.6	0.188059
9	8.65	699	91	0.119258	6	1.2599	10.9	8.4	8.65	698.5	6.3	0.11204
10	9.3	738	96.1	0.129183	5	1.10103	9.6	7.4	9.3	737.7	4.9	0.127518

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.000 5.200 8.100 5.850 9.800 6.550 15.400 6.850 3.700 7.350 4.700 8.150 13.700 8.450 16.200 8.650 8.200 9.300 16.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
5.796 1.800 5.990 2.100 6.435 0.800 6.518 0.300 6.610 0.400 6.758 5.500 6.895 12.600 6.955 8.700 7.045 6.000 7.226 2.800 7.346 0.900 7.389 5.100 7.502 9.700 7.572 1.300 7.650 0.700 7.854 23.000 8.403 1.500 8.915 12.500 9.206 0.200 9.411 4.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 2.700 5.200 5.400 5.850 10.600 6.550 17.400 6.850 5.500 7.350 9.200 8.150 13.100 8.450 17.000 8.650 8.700 9.300 10.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 1.300 5.200 2.400 5.803 1.200 5.850 2.600 6.007 1.300 6.449 1.600 6.550 2.300 6.850 4.800 6.960 12.600 7.097 4.900 7.254 1.400 7.350 3.700 7.496 9.600 7.580 0.900 7.663 0.100 7.900 8.600 8.150 1.900 8.367 0.400 8.450 1.500 8.848 19.100 9.204 14.700 9.357 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
5.798 1.200 6.005 1.200 6.420 0.500 6.515 0.300 6.633 0.300 6.790 3.200 6.920 9.600 7.061 4.200 7.231 1.500 7.335 0.900 7.377 0.600 7.437 0.900 7.484 2.400 7.572 2.500 7.666 0.500 7.767 0.700 7.881 13.500 8.376 0.700 8.561 4.900 8.874 22.800 9.227 6.300 9.378 21.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 22 Return to Lane Navigator
4.550 3.200 5.200 5.700 5.850 9.000 6.550 16.500 6.850 3.900 7.350 8.000 8.150 14.200 8.450 19.000 8.650 10.900 9.300 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Shortfin Mako Shark ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Shortfin Mako Shark ---- Go to Display Gel Lane Navigator.