Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Starry Flounder  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Platichthys stellatus
FDA Seafood List Common Name: Starry Flounder
FDA Seafood List Market Name: Flounder
Unique IEF Gel Code: 19950809gel13
Unique Web Code: sfg01Srr
Tissue Aliquot Code: platstel H95-005 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5433.80.190521175.7038514.511.23.547.716.80.192107
24.5518316.20.36657952.800517.15.54.55183.24.70.333286
35.226523.50.44324243.320748.46.55.2264.74.40.43052
45.8542037.20.61707944.1521610.58.25.85420.53.70.635118
56.5558551.90.44936653.517348.96.96.55584.34.60.430466
66.8562455.30.29609273.157486.26.856246.60.271894
77.3571663.50.47050475.5797914.1117.35713.97.10.445149
88.1587477.50.075495491.128962.92.28.15873.58.80.0726854
98.4593382.70.1280261.423053.62.88.45933.36.30.127457
108.6597286.20.1577551.516783.838.65971.55.50.156005
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3109797.30.56825587.1335318.1149.31095.77.60.5287
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0231119.80.70891567.3421115.111.44.023111.65.60.738531
24.34115513.70.050205750.4100110.80.64.341154.74.90.0472306
34.46317215.20.020724440.1408510.30.24.463172.13.60.0223396
44.62819417.20.52544465.3914911.18.34.628194.260.507383
54.91823120.50.087306491.359722.82.14.918228.79.30.0827974
65.00424221.50.097416540.6319651.315.004242.44.10.0859524
75.16926323.30.0485473110.8029251.71.25.169263.211.10.0408754
85.23827624.50.033057650.2173940.40.35.238276.44.80.0254501
95.309293260.049446940.3387880.70.55.309293.64.30.0448895
105.41731928.30.42703642.926364.55.417318.63.80.435628
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.47233229.40.31017341.96101435.472332.43.50.312905
125.60536432.30.73780345.2323910.88.15.605364.540.738226
135.6637733.40.53177165.1746810.785.66377.25.90.494139
145.74339735.20.133507101.983044.13.15.743396.79.90.113225
155.86942737.90.021910830.1123480.20.25.869428.22.60.0245387
166.02546441.10.53488633.029336.24.76.025463.83.20.534359
176.16449744.10.50108987.0947414.6116.164498.87.60.530101
186.28652646.60.0955811101.369682.82.16.286522.610.10.0762852
196.37954848.60.056791840.3531360.70.56.379546.940.0501751
206.41355649.30.40024431.837973.82.86.413556.52.50.410782
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.88363456.20.1451530.8105811.71.36.883633.53.10.147448
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.53430.154693123.054939.26.93.537.111.80.146223
24.5518516.40.24858952.179246.54.94.551855.10.240274
35.226923.80.3021252.633137.965.2268.85.10.293418
45.8542537.60.5506843.593610.88.25.85425.33.70.550114
56.5559252.40.38532253.12019.47.16.555924.60.379188
66.85632560.21471462.212056.656.85632.26.30.198245
77.35722640.43571264.8647214.6117.35720.76.50.425145
88.1587877.80.084612681.178793.52.78.15877.580.0828035
98.4594283.40.1798462.0044864.68.45941.76.50.174776
108.6598086.80.19598861.943045.84.48.65979.75.80.189899
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.31106980.57131776.5810919.714.99.31104.47.20.513622
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 19
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 21
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.53430.145898112.630463.42.93.537.210.90.136244
23.9921089.60.6710866.8580197.63.992107.75.60.696971
34.29115313.60.028246250.2838180.40.34.291151.75.10.0312113
44.40417015.10.017296430.09994370.10.14.404169.43.20.0178938
54.55192170.42573885.621037.46.24.55191.87.60.416079
64.85422920.30.071758930.1842480.20.24.854218.13.10.0336625
74.93723921.20.10026132.716143.634.937236.213.10.117302
85.2271240.19108782.450943.22.75.2270.87.50.183703
95.28229025.70.023097260.1833580.20.25.282293.660.0172996
105.41532128.50.41927942.711293.635.415321.43.60.427233
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.4833629.80.24524452.115062.82.35.48336.54.70.251354
125.60936632.50.66720144.973896.55.55.609366.34.20.668057
135.66938033.70.47828965.013956.65.55.669380.56.20.452734
145.76440235.70.12892761.198431.61.35.764402.15.80.117501
155.8542237.40.33239442.097052.72.35.85421.93.60.331933
166.04846941.60.64887622.804473.73.16.048469.22.40.669582
176.20450644.90.51467865.3534875.96.204506.35.90.514682
186.31853347.30.141993153.215874.23.66.318525.314.70.123315
196.40755449.20.058438640.2607130.30.36.407555.23.60.040501
206.46156750.30.47554221.73292.31.96.461566.720.494287
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.5558852.20.17645961.978592.62.26.55586.26.30.177979
226.8563055.90.1631161.632672.11.86.85630.66.20.148979
236.93864657.30.18779730.9442091.216.938645.73.10.171767
247.35721640.44996464.873796.45.47.35720.46.40.427334
258.1587477.60.07618173.387714.43.78.15864.116.80.11375
268.4593783.10.11446681.591482.11.88.45937.48.40.106747
278.6597986.90.12515461.399291.81.58.659796.40.12244
289.3110698.10.69400678.0396610.58.99.31104.77.30.620191
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 20
LANE TYPE: Specimen
ACTUAL GEL LANE: 22
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.03811410.10.71124757.0668715.511.24.038114.15.40.742347
24.333158140.050847940.3542030.80.64.333157.94.10.0485407
34.44617515.50.029656840.18720.40.34.446175.73.80.0281099
44.61719917.60.5574765.5229612.18.74.617199.35.70.544108
54.831226200.065992140.4369810.74.8312263.80.0652605
64.919237210.066870330.2359360.50.44.919237.42.70.05017
74.99824721.90.076876491.090752.41.74.998247.58.50.0723156
85.17226923.80.029062440.2267970.50.45.172270.24.40.0287927
95.25828625.30.03034130.1936860.40.35.258284.63.20.0337758
105.33130326.80.089305150.7785361.71.25.331302.95.20.0847047
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.46433429.60.5929333.144426.955.464333.72.90.603681
125.5535431.40.42843832.341255.13.75.55354.23.10.419734
135.64837733.40.68280744.401389.675.648377.23.80.65595
145.71339234.70.48426174.176349.26.65.713392.46.60.359362
155.87543038.10.0902324182.961786.54.75.875415.7180.0928355
166.06347642.20.60013322.173694.83.46.063475.620.615312
176.19850945.10.54148444.249069.36.76.198509.24.40.541641
186.353447.30.161853133.258947.15.26.3531.413.40.137397
196.378553490.11110930.3824960.80.66.378553.92.90.0755675
206.43556750.20.44902221.741373.82.86.435566.62.10.47224
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.87764056.70.14203730.6977931.51.16.877640.52.70.144811
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 21
LANE TYPE: pI Markers
ACTUAL GEL LANE: 23
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5322.80.177485113.2682210.98.93.533.410.90.169527
24.5518816.60.20163362.1116275.74.55187.960.197189
35.227324.10.25146762.464048.26.75.2272.95.60.249537
45.8542537.60.55064843.500911.69.55.85425.33.60.550288
56.5560153.10.39268842.828059.47.76.55600.54.10.386808
66.8563956.50.18078561.755445.84.86.85638.660.165314
77.3573064.50.44863564.3462414.511.87.35729.65.60.440729
88.1588578.20.076539881.033493.42.88.15885.97.90.0735397
98.4594783.70.14776961.459814.948.45946.85.80.143044
108.6598687.20.13641961.331414.43.68.65984.95.60.133735
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3111298.30.41947495.964719.816.29.31110.68.90.378127
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 19
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 21
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 20
LANE TYPE: Specimen
ACTUAL GEL LANE: 22
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 21
LANE TYPE: pI Markers
ACTUAL GEL LANE: 23
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln