RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Bonito ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sarda chiliensis
FDA Seafood List Common Name: Pacific Bonito
FDA Seafood List Market Name: Bonito
Unique IEF Gel Code: 19981215gel01
Unique Web Code: sbh01Srr
Tissue Aliquot Code: sardchil J9-003 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	34	4.3	0.0769255	5	0.616818	9	6.4	4.55	33.8	4.6	0.0750856
2	5.2	123	15.5	0.0677594	5	0.618237	9	6.4	5.2	123.3	5.2	0.0675166
3	5.85	230	29	0.042986	5	0.37929	5.5	3.9	5.85	230.9	5.4	0.0397903
4	6.55	373	47.1	0.125422	6	1.32955	19.4	13.8	6.55	372.5	6.4	0.116527
5	6.85	404	51	0.0311014	5	0.260543	3.8	2.7	6.85	404.7	5	0.0294122
6	7.35	471	59.5	0.0729885	4	0.485647	7.1	5	7.35	471	3.9	0.0697427
7	8.15	592	74.7	0.0916977	7	1.06102	15.5	11	8.15	591.7	6.8	0.0874059
8	8.45	658	83.1	0.120784	6	1.38756	20.2	14.4	8.45	656.3	6.4	0.121691
9	8.65	711	89.8	0.104674	4	0.717109	10.5	7.4	8.65	710.8	4	0.102189

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.664	193	24.5	0.0321128	5	0.246532	0.7	0.6	5.664	192.8	4.7	0.0297036
2	6.18	293	37.2	0.033585	3	0.147529	0.4	0.4	6.18	292.8	2.8	0.0295124
3	6.478	355	45.1	0.0338431	3	0.149965	0.4	0.4	6.478	355.3	2.6	0.0330496
4	6.598	375	47.6	0.398704	2	1.24943	3.4	3.1	6.598	374.4	1.7	0.421973
5	6.769	393	49.9	0.0699691	5	0.673787	1.8	1.7	6.769	394.9	4.6	0.0827295
6	6.845	401	50.9	0.28487	2	0.998778	2.7	2.5	6.845	401.4	2.2	0.25127
7	6.943	414	52.5	1.8449	1	4.09386	11	10.1	6.943	414.2	1.2	1.90952
8	7.246	455	57.7	2.35693	3	16.5949	44.7	40.8	7.246	454.1	3.5	2.67515
9	7.456	485	61.5	0.157698	3	0.779144	2.1	1.9	7.456	485.1	3.1	0.141815
10	7.735	527	66.9	0.0142015	5	0.14245	0.4	0.3	7.735	528.2	5.3	0.0150797
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.808	538	68.3	0.0702514	3	0.370935	1	0.9	7.808	538.3	3.3	0.0643368
12	7.908	553	70.2	2.38337	2	7.5552	20.3	18.6	7.908	553.9	1.6	2.66077
13	8.167	593	75.3	0.122701	4	0.894046	2.4	2.2	8.167	592.7	4.2	0.120268
14	8.248	610	77.4	0.368076	4	2.47377	6.7	6.1	8.248	610.2	3.9	0.358259
15	8.433	649	82.4	0.0171963	4	0.0995273	0.3	0.2	8.433	647.8	3.7	0.0153428
16	8.548	679	86.2	0.0299678	6	0.245658	0.7	0.6	8.548	679.6	5.7	0.0244473
17	8.867	765	97.1	0.0416225	6	0.416837	1.1	1	8.867	764.3	6.2	0.0380656

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	26	3.3	0.0760943	4	0.471198	6.9	4.9	4.55	26.7	3.7	0.0725565
2	5.2	109	13.9	0.0590586	5	0.450971	6.6	4.7	5.2	109.2	4.5	0.0559561
3	5.85	222	28.2	0.0463515	5	0.402114	5.9	4.1	5.85	221.8	5.3	0.0424815
4	6.55	370	47	0.108695	7	1.25976	18.4	13	6.55	368.5	7.2	0.0993982
5	6.85	402	51.1	0.024834	6	0.219042	3.2	2.3	6.85	402.2	5.9	0.0210977
6	7.35	470	59.7	0.0579747	4	0.418367	6.1	4.3	7.35	470	4.4	0.0530962
7	8.15	590	75	0.123174	6	1.25097	18.3	12.9	8.15	589.9	6	0.117845
8	8.45	650	82.6	0.129555	7	1.54685	22.6	16	8.45	649.4	6.9	0.126552
9	8.65	705	89.6	0.121948	4	0.810569	11.9	8.4	8.65	704.2	4	0.113648

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	36	4.6	0.0334289	4	0.201926	0.3	0.3	4.55	36	3.6	0.0315271
2	5.092	106	13.5	0.0235972	6	0.226254	0.3	0.3	5.092	105.8	5.9	0.0214816
3	5.2	120	15.3	0.039257	5	0.320905	0.5	0.4	5.2	119.3	4.7	0.0385442
4	5.673	195	24.8	0.0320667	6	0.321419	0.5	0.4	5.673	193.6	5.8	0.0312639
5	6	254	32.4	0.0147925	6	0.138169	0.2	0.2	6	254.2	5.6	0.0138052
6	6.087	272	34.6	0.0208565	3	0.116798	0.2	0.2	6.087	271.6	3.4	0.0192681
7	6.271	310	39.5	0.0196837	4	0.123646	0.2	0.2	6.271	309.2	3.8	0.0182227
8	6.547	367	46.8	0.042533	5	0.351861	0.5	0.5	6.547	366	4.9	0.0407567
9	6.704	385	49	0.870447	4	5.01016	7.2	6.9	6.704	382.9	3.7	0.756925
10	6.838	400	51	0.120384	6	1.55968	2.3	2.1	6.838	402.6	6.2	0.141238
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.9	408	52	1.72513	2	4.71494	6.8	6.5	6.9	408	1.6	1.63679
12	6.983	419	53.4	2.28314	2	7.16545	10.3	9.8	6.983	419.4	1.8	2.29734
13	7.253	455	58	2.31191	3	14.4231	20.8	19.8	7.253	454.6	3.1	2.59358
14	7.35	468	59.6	0.275742	4	1.95799	2.8	2.7	7.35	468	4	0.273729
15	7.485	489	62.3	0.304727	3	1.60345	2.3	2.2	7.485	489	3.3	0.274183
16	7.737	528	67.3	0.0492429	10	0.707099	1	1	7.737	528.2	9.5	0.0418328
17	7.815	540	68.8	0.126211	3	0.601092	0.9	0.8	7.815	540.5	2.9	0.11882
18	7.918	556	70.8	2.33061	3	11.2881	16.3	15.5	7.918	556.7	2.6	2.43305
19	8.15	592	75.4	0.17624	5	1.47439	2.1	2	8.15	592.4	4.8	0.171544
20	8.252	612	78	1.49014	3	7.8487	11.3	10.8	8.252	612.3	3	1.49081
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	651	82.9	0.0512331	5	0.420818	0.6	0.6	8.45	650.9	4.6	0.0520876
22	8.554	679	86.5	0.181151	9	2.9466	4.3	4	8.554	679.6	9.5	0.175048
23	8.609	694	88.4	0.183253	4	0.88924	1.3	1.2	8.609	692	4.2	0.120465
24	8.65	705	89.8	0.287973	9	3.86292	5.6	5.3	8.65	703.9	8.5	0.255736
25	8.857	761	96.9	0.141328	4	0.963987	1.4	1.3	8.857	760.5	4.2	0.128755

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.694	198	25.3	0.0611481	6	0.611476	0.6	0.6	5.694	196.5	5.9	0.0579835
2	5.784	213	27.2	0.0341066	5	0.280134	0.3	0.3	5.784	214.3	5.3	0.0297334
3	5.994	253	32.3	0.0283667	4	0.201801	0.2	0.2	5.994	252.8	4.3	0.0264421
4	6.083	271	34.6	0.0281651	3	0.145144	0.1	0.1	6.083	270	3	0.0274921
5	6.246	304	38.8	0.0526389	3	0.262977	0.3	0.3	6.246	303.3	2.8	0.0531357
6	6.556	366	46.7	0.0933149	4	0.677007	0.7	0.6	6.556	366.2	4.5	0.0854659
7	6.717	385	49.2	2.12826	2	8.50682	8.4	8.1	6.717	385.4	2.3	2.10612
8	6.86	402	51.3	0.189154	6	2.2281	2.2	2.1	6.86	405.3	6.3	0.200129
9	6.92	410	52.4	1.911	2	6.73088	6.7	6.4	6.92	409.7	2	1.91395
10	7.011	422	53.9	2.14867	3	10.5358	10.5	10.1	7.011	422.3	2.7	2.21455
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.267	456	58.2	2.30461	4	18.1142	18	17.3	7.267	456.4	4.2	2.44508
12	7.37	470	60	0.184452	4	1.3861	1.4	1.3	7.37	469.4	3.8	0.203691
13	7.517	492	62.8	0.376447	4	2.31029	2.3	2.2	7.517	491.6	3.6	0.362942
14	7.792	533	68.1	0.0590742	5	0.497157	0.5	0.5	7.792	533.5	5	0.0560683
15	7.865	544	69.5	0.129857	2	0.520858	0.5	0.5	7.865	543.5	2.5	0.119518
16	7.953	557	71.1	2.35655	3	13.9938	13.9	13.4	7.953	558.3	3.2	2.45226
17	8.224	601	76.8	0.257374	5	2.29542	2.3	2.2	8.224	600.7	5.1	0.255343
18	8.331	622	79.4	2.33005	4	17.6455	17.5	16.9	8.331	623.5	4	2.5034
19	8.503	661	84.4	0.019725	5	0.200058	0.2	0.2	8.503	658.9	4.7	0.0242286
20	8.586	685	87.5	0.646324	8	8.00311	7.9	7.6	8.586	686	7.8	0.576554
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.634	699	89.3	0.451711	5	4.11168	4.1	3.9	8.634	699.2	5.4	0.426182
22	8.672	710	90.7	0.150956	1	0.231163	0.2	0.2	8.672	710.2	1.4	0.0921002
23	8.852	762	97.3	0.17376	4	1.21035	1.2	1.2	8.852	761.4	4.2	0.162255

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.0664247	4	0.506902	7.3	5.3	4.55	28.3	4.3	0.0661008
2	5.2	114	14.6	0.068872	4	0.493169	7.1	5.1	5.2	113.7	4.1	0.0670877
3	5.85	227	29	0.0394331	5	0.344644	5	3.6	5.85	226.5	5.4	0.0363366
4	6.55	365	46.6	0.0886618	9	1.37896	19.9	14.3	6.55	362.5	9.3	0.083591
5	6.85	402	51.3	0.0272322	6	0.260138	3.7	2.7	6.85	400.6	5.6	0.0260947
6	7.35	468	59.8	0.0545814	4	0.381539	5.5	4	7.35	467.9	4.2	0.0514755
7	8.15	583	74.5	0.107546	6	1.06517	15.3	11.1	8.15	582.6	5.8	0.102975
8	8.45	642	82	0.136708	8	1.72887	24.9	18	8.45	643.1	7.8	0.125634
9	8.65	704	89.9	0.112033	4	0.784672	11.3	8.2	8.65	703.6	4.3	0.103257

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.000 5.200 9.000 5.850 5.500 6.550 19.400 6.850 3.800 7.350 7.100 8.150 15.500 8.450 20.200 8.650 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
5.664 0.700 6.180 0.400 6.478 0.400 6.598 3.400 6.769 1.800 6.845 2.700 6.943 11.000 7.246 44.700 7.456 2.100 7.735 0.400 7.808 1.000 7.908 20.300 8.167 2.400 8.248 6.700 8.433 0.300 8.548 0.700 8.867 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 17 Return to Lane Navigator
4.550 6.900 5.200 6.600 5.850 5.900 6.550 18.400 6.850 3.200 7.350 6.100 8.150 18.300 8.450 22.600 8.650 11.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 0.300 5.092 0.300 5.200 0.500 5.673 0.500 6.000 0.200 6.087 0.200 6.271 0.200 6.547 0.500 6.704 7.200 6.838 2.300 6.900 6.800 6.983 10.300 7.253 20.800 7.350 2.800 7.485 2.300 7.737 1.000 7.815 0.900 7.918 16.300 8.150 2.100 8.252 11.300 8.450 0.600 8.554 4.300 8.609 1.300 8.650 5.600 8.857 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator
5.694 0.600 5.784 0.300 5.994 0.200 6.083 0.100 6.246 0.300 6.556 0.700 6.717 8.400 6.860 2.200 6.920 6.700 7.011 10.500 7.267 18.000 7.370 1.400 7.517 2.300 7.792 0.500 7.865 0.500 7.953 13.900 8.224 2.300 8.331 17.500 8.503 0.200 8.586 7.900 8.634 4.100 8.672 0.200 8.852 1.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 23 Return to Lane Navigator
4.550 7.300 5.200 7.100 5.850 5.000 6.550 19.900 6.850 3.700 7.350 5.500 8.150 15.300 8.450 24.900 8.650 11.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Bonito ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Bonito ---- Go to Display Gel Lane Navigator.