Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19971105gel02
Unique Web Code: rsh03Srr
Tissue Aliquot Code: lutjcamp D97-003 #07p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55425.10.074909250.6848817.95.94.5543.45.40.0715435
25.212214.80.075042860.6901585.95.2122.35.70.0677444
35.8523428.40.17221741.1596113.49.95.85234.43.70.175607
46.5539748.20.1570961.4866217.112.76.55396.35.60.148546
56.8543052.20.021619560.2054022.41.86.85430.55.90.019494
67.35510620.060152560.5910416.85.17.35508.860.0556518
78.1564378.10.086731681.1348113.19.78.15642.97.70.0835969
88.4571086.30.086382571.0576312.29.18.45709.470.0855468
98.6575892.10.063582850.5566166.44.88.65757.45.10.0611781
109.380497.70.12527851.102712.79.59.3803.75.30.116909
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.388263.20.041191660.402531.41.34.38825.45.90.0385618
25.20912515.20.016380550.133510.50.45.209125.35.30.0142371
35.32114417.50.080213930.412961.41.35.321144.130.0781949
45.66820324.60.24084852.171037.66.85.668202.65.40.224918
56.00527132.90.51501343.0698210.79.76.005271.83.50.493945
66.10529535.80.26305752.0018876.36.105294.74.70.238003
76.19731738.50.13953551.304054.64.16.197316.550.146685
86.21832239.10.17934630.85719332.76.218323.43.30.146642
96.30134241.50.036635360.3859471.31.26.301342.55.70.0384397
106.38936344.10.049968430.3042771.116.389363.83.40.0511376
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.44837745.80.010477140.06043280.20.26.448377.63.60.009418
126.48538646.80.031259630.1910080.70.66.485386.93.50.0308114
136.51439347.70.17221330.86801232.76.514392.62.80.173224
146.62541049.80.4867332.767899.78.76.625409.93.20.492996
156.69641850.70.33929442.206797.776.696418.440.311746
166.83743452.70.17100271.894476.666.837433.46.90.15422
177.24449560.10.13450740.8837943.12.87.244496.14.20.117376
187.66656368.30.0454809110.6502482.32.17.666565.310.90.0336538
197.80658671.10.33336552.792039.88.87.806585.75.10.306267
207.9460873.80.236616115.2701818.416.67.94612.210.80.275553
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55485.80.068701760.7004638.26.14.5549.460.0659745
25.2124150.057830270.6644477.85.85.2123.97.10.053162
35.8523328.30.13298551.10595139.65.85232.84.50.137284
46.5540549.20.11863181.5633918.313.66.55403.88.20.108211
56.8544053.40.02728380.341982436.85441.97.70.0250751
67.35511620.069299550.563186.64.97.35510.94.80.0658294
78.1564177.80.086364470.99546611.78.68.15641.46.70.0834494
88.4571086.20.065635570.8306839.77.28.45708.87.30.0640878
98.6575791.90.065505940.4748445.64.18.65757.14.20.0637738
109.380797.90.14628251.2856115.111.29.3806.55.10.140984
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.3623340.026415350.2452360.80.84.362325.20.0267331
24.55556.70.047990550.4281641.51.44.5554.65.20.0463856
35.0211013.30.085241660.8413652.92.85.02110.75.90.0808219
45.213115.90.054331490.8505252.92.85.2128.59.40.0511242
55.32615218.40.031328270.3874391.31.35.326149.67.20.0302486
65.62120124.40.0475164100.8251222.82.75.621199.110.30.0450021
75.85239290.066569240.4735571.61.65.85238.240.0660831
86.0328234.20.35589552.784999.59.16.03281.54.90.323535
96.13830837.40.14200581.866756.46.16.138306.18.40.124666
106.24733440.50.10872181.61025.55.36.247331.67.80.11621
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.31835142.60.025131740.1515930.50.56.318353.33.80.0224068
126.40537245.10.030468140.2020730.70.76.405371.93.70.0306769
136.45538446.60.018289730.1200950.40.46.455385.63.20.0208563
146.50139547.90.045713260.4493531.51.56.501394.65.70.0446503
156.53140248.80.17196330.9293243.23.16.531402.33.20.166006
166.67242151.10.42287342.89839.99.56.672420.43.90.421982
176.74843052.20.2744352.30057.87.66.748429.850.258105
186.86444453.90.15084261.601555.45.36.864443.260.150125
197.292503610.1334330.7481462.52.57.292502.53.20.129928
207.35511620.078605650.7361442.52.47.35511.55.20.0801013
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.68356668.70.030328350.2389860.80.87.683565.84.70.0285564
227.82358971.50.29889252.3507487.77.823589.34.70.281739
237.96861374.40.228387104.0976413.913.47.968615.29.60.240688
248.15643780.0481855110.9790053.33.28.15639.211.10.0497956
258.4571987.30.034570660.3291081.11.18.45718.95.50.033732
268.6576392.60.026668740.2032710.70.78.65762.24.40.0260951
279.380797.90.06369380.7470712.52.59.3805.47.50.0560445
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.405344.10.049160450.4399561.41.34.40534.35.30.0468026
25.03411213.60.10943640.6625682.225.03411240.0932561
35.20313316.10.017875140.5292531.71.65.203121.214.40.0207687
45.32615418.70.072974530.4264491.41.35.326154.23.50.0696334
55.66821225.70.12104781.5335554.55.668210.17.70.11263
66.008280340.49556732.7683398.26.008280.13.30.478941
76.1130436.90.23851541.615625.34.86.11303.740.229374
86.21232839.80.21221172.446887.26.212326.46.50.211296
96.2834441.70.045024860.4993121.61.56.28345.360.0470148
106.37436644.40.050485350.398861.31.26.374367.95.10.043763
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.45938646.80.018411120.07505740.20.26.459385.72.40.0175472
126.48939347.70.03662630.2181660.70.66.489394.43.40.0358669
136.53240348.90.22279331.121453.73.36.532402.92.90.219554
146.67342251.20.50267343.2669710.79.76.673421.93.70.502719
156.74943152.30.38475352.79189.18.36.7494314.50.347603
166.87144654.10.23939982.998179.88.96.871445.780.212704
177.26850260.90.1704851.2309943.67.268502.74.50.152971
187.70657269.40.032113550.2553250.80.87.7065714.60.0314597
197.85259672.30.30484662.93829.68.77.852595.75.90.280548
207.9861774.90.36233974.4514314.513.27.98616.77.10.353217
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55506.10.095234760.95843297.44.5551.36.40.0850068
25.213516.30.06926370.85504686.65.21347.10.0682049
35.85248300.13635761.336612.510.45.85247.15.80.131044
46.5540949.50.100513111.9061317.814.86.55404.811.40.0943938
56.8544553.90.044459360.4419224.13.46.85444.96.20.0403717
67.3551762.60.086042560.9172838.67.17.35516.46.30.0827226
78.1564878.50.10070981.4378513.411.28.15647.48.40.0970266
88.4571586.60.1152171.4427213.511.28.457147.30.112269
98.6576492.50.078175750.7307366.85.78.65763.35.40.0762359
109.381498.50.10611630.6643816.25.29.3813.73.50.107998
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 97
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln