RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19971105gel01
Unique Web Code: rsg03Srr
Tissue Aliquot Code: lutjcamp D97-003 #07p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	73	3.3	0.0730819	19	3.1332	6.4	4.9	3.5	82.6	18.8	0.093856
2	4.55	417	18.9	0.278922	17	7.97106	16.2	12.4	4.55	416.8	17.3	0.259308
3	5.2	601	27.2	0.211681	16	6.3137	12.8	9.8	5.2	598.1	16.2	0.219594
4	5.85	908	41	0.350904	11	6.94662	14.1	10.8	5.85	906.1	11.3	0.345794
5	6.55	1244	56.2	0.15883	8	2.0834	4.2	3.2	6.55	1242	8.1	0.144379
6	6.85	1327	60	0.191272	10	3.00534	6.1	4.7	6.85	1327.4	9.9	0.171009
7	7.35	1508	68.2	0.248122	11	4.37106	8.9	6.8	7.35	1505.6	10.5	0.234425
8	8.15	1811	81.9	0.110871	18	3.39583	6.9	5.3	8.15	1809.3	17.9	0.107185
9	8.45	1919	86.8	0.145584	9	2.34114	4.7	3.6	8.45	1919.9	9.2	0.144246
10	8.65	1989	89.9	0.173136	11	3.36724	6.8	5.2	8.65	1988.9	11.5	0.165343
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2195	99.2	0.367551	10	6.38574	12.9	9.9	9.3	2194.5	10.1	0.355245

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	63	2.8	0.105732	16	2.68464	3.8	2.7	3.5	65.5	15.7	0.0966423
2	4.279	302	13.6	0.222283	9	3.41781	4.8	3.5	4.279	301.9	8.8	0.219493
3	4.55	385	17.4	0.252828	12	4.91126	7	5	4.55	388	11.7	0.236974
4	4.95	492	22.2	0.104779	7	1.39012	2	1.4	4.95	491.7	7.4	0.106244
5	5.2	559	25.2	0.186649	12	3.5896	5.1	3.6	5.2	559	11.5	0.175629
6	5.317	615	27.8	0.195677	12	3.754	5.3	3.8	5.317	613.9	11.6	0.182375
7	5.629	765	34.6	0.260964	12	5.42938	7.7	5.5	5.629	768	11.6	0.265189
8	5.85	871	39.3	0.222843	10	4.07514	5.8	4.1	5.85	872.4	10.4	0.221373
9	5.987	941	42.5	0.463216	9	7.2605	10.3	7.3	5.987	943.1	9	0.457321
10	6.081	989	44.7	0.276963	8	4.01493	5.7	4.1	6.081	988.1	8.4	0.269118
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.152	1025	46.3	0.0946664	10	1.61894	2.3	1.6	6.152	1025.3	9.6	0.09468
12	6.187	1043	47.1	0.11593	7	1.40115	2	1.4	6.187	1044.5	7	0.112183
13	6.43	1167	52.7	0.070261	14	1.63245	2.3	1.6	6.43	1170.5	14.2	0.0647883
14	6.55	1228	55.5	0.175137	5	1.52593	2.2	1.5	6.55	1227.6	5.2	0.167132
15	6.631	1253	56.6	0.162571	5	1.18758	1.7	1.2	6.631	1252.5	4.5	0.14844
16	6.698	1274	57.5	0.302674	12	5.66844	8	5.7	6.698	1275.3	12	0.267357
17	6.85	1321	59.7	0.0952647	7	1.11964	1.6	1.1	6.85	1322	7	0.0901387
18	7.297	1480	66.8	0.108911	6	0.98389	1.4	1	7.297	1478.6	5.5	0.100787
19	7.35	1499	67.7	0.197516	9	3.2803	4.7	3.3	7.35	1498.5	9.3	0.198329
20	8.15	1801	81.3	0.0333483	18	1.2422	1.8	1.3	8.15	1801	17.7	0.0396048
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	1917	86.6	0.149146	11	2.79133	4	2.8	8.45	1918.4	10.8	0.146364
22	8.65	1991	89.9	0.133485	11	2.48668	3.5	2.5	8.65	1990.7	11	0.127827
23	9.3	2195	99.1	0.378547	8	5.02766	7.1	5.1	9.3	2195	7.5	0.376465

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.393	325	14.7	0.237846	9	3.97831	8.2	4.9	4.393	325.2	9.3	0.24038
2	5.166	533	24.1	0.0794302	10	1.36153	2.8	1.7	5.166	531.6	10.2	0.0753558
3	5.437	658	29.8	0.154428	15	3.91811	8.1	4.8	5.437	653.3	14.5	0.152453
4	5.729	801	36.2	0.151409	21	8.05625	16.6	10	5.729	786.2	21.2	0.214536
5	5.932	903	40.9	0.114616	11	2.17186	4.5	2.7	5.932	902.5	11.4	0.10782
6	6.055	967	43.8	0.468659	8	6.19272	12.8	7.7	6.055	967.4	7.5	0.463254
7	6.124	1003	45.4	0.354701	9	5.08979	10.5	6.3	6.124	1002.5	8.6	0.333406
8	6.191	1038	47	0.136323	11	2.42344	5	3	6.191	1035.5	11	0.124617
9	6.222	1054	47.7	0.172855	6	1.66944	3.4	2.1	6.222	1053.7	5.8	0.161947
10	6.543	1221	55.2	0.206799	6	2.13418	4.4	2.6	6.543	1223.3	6.2	0.194894
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.626	1247	56.4	0.240443	5	1.69926	3.5	2.1	6.626	1248.2	4.7	0.205253
12	6.687	1265	57.2	0.320274	12	6.05738	12.5	7.5	6.687	1267.9	11.9	0.286795
13	7.265	1462	66.2	0.140479	4	1.03821	2.1	1.3	7.265	1461.3	4.3	0.136264
14	7.827	1670	75.6	0.0812527	9	1.29135	2.7	1.6	7.827	1669.6	8.8	0.0830002
15	8.528	1935	87.6	0.0611669	13	1.36052	2.8	1.7	8.528	1934.8	13.3	0.0578185

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	53	2.4	0.0896302	13	2.0263	4.4	3.3	3.5	55.6	13	0.0877166
2	4.55	361	16.4	0.285595	11	5.23021	11.4	8.5	4.55	364.7	11	0.268857
3	5.2	527	23.9	0.274307	11	4.88437	10.6	7.9	5.2	529.1	10.6	0.258805
4	5.85	852	38.6	0.309932	12	6.56964	14.3	10.6	5.85	852.8	11.9	0.312096
5	6.55	1223	55.4	0.14981	9	2.24115	4.9	3.6	6.55	1221.7	9	0.140372
6	6.85	1307	59.2	0.197981	10	3.15096	6.8	5.1	6.85	1307.6	10.1	0.176739
7	7.35	1488	67.4	0.267493	10	4.57033	9.9	7.4	7.35	1485.4	10.1	0.256048
8	8.15	1781	80.7	0.0839529	16	2.41464	5.2	3.9	8.15	1783	16.3	0.0837524
9	8.45	1899	86	0.186528	13	4.0818	8.9	6.6	8.45	1898.2	13.2	0.175042
10	8.65	1968	89.2	0.162814	12	3.11089	6.8	5	8.65	1965	11.5	0.152511
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2138	96.9	0.204406	21	7.76501	16.9	12.6	9.3	2144.4	20.7	0.211962

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.402	325	14.7	0.24559	9	3.78558	9.1	5.6	4.402	326	8.6	0.247458
2	4.773	427	19.3	0.0687808	21	2.41198	5.8	3.6	4.773	429.4	20.8	0.0654995
3	5.158	527	23.9	0.0720724	9	1.13902	2.7	1.7	5.158	527.3	8.7	0.0735927
4	5.418	645	29.2	0.166762	15	4.24379	10.2	6.3	5.418	642	14.9	0.160699
5	5.703	785	35.5	0.0147161	5	0.180556	0.4	0.3	5.703	787.8	5.2	0.0197738
6	5.922	895	40.5	0.0905514	13	2.12011	5.1	3.2	5.922	899.3	13.3	0.0897672
7	6.026	949	43	0.560204	8	7.91532	19	11.8	6.026	949.7	8.1	0.553504
8	6.103	989	44.8	0.364016	9	5.36848	12.9	8	6.103	989.3	8.8	0.342981
9	6.16	1019	46.1	0.145096	10	2.55204	6.1	3.8	6.16	1019.9	9.7	0.148146
10	6.191	1035	46.9	0.17052	6	1.65906	4	2.5	6.191	1035.4	5.9	0.158663
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.515	1204	54.5	0.145321	4	1.62609	3.9	2.4	6.515	1206.2	4.2	0.220969
12	6.575	1229	55.6	0.150285	4	1.02336	2.5	1.5	6.575	1228.7	4.3	0.135589
13	6.646	1249	56.5	0.327893	10	4.9025	11.8	7.3	6.646	1251.4	10.2	0.270436
14	7.247	1451	65.7	0.13918	4	1.02465	2.5	1.5	7.247	1450.9	4.2	0.137072
15	7.813	1660	75.1	0.0959158	10	1.64247	3.9	2.4	7.813	1659.3	10	0.0923035

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	56	2.5	0.129091	13	2.88343	5.4	4.1	3.5	60.3	13	0.125294
2	4.55	374	16.9	0.384734	10	6.43245	12	9.2	4.55	375.1	10.3	0.351474
3	5.2	546	24.7	0.380117	8	5.29843	9.9	7.6	5.2	545.7	8	0.374031
4	5.85	860	38.9	0.424595	10	6.9528	13	9.9	5.85	861.7	9.5	0.411131
5	6.55	1218	55.2	0.140072	8	1.94736	3.6	2.8	6.55	1215.8	8.3	0.132738
6	6.85	1302	59	0.192395	9	2.9203	5.5	4.2	6.85	1303.1	9.3	0.176647
7	7.35	1486	67.3	0.267839	10	4.67474	8.7	6.7	7.35	1483.6	10.2	0.257693
8	8.15	1786	80.9	0.0952748	22	3.43093	6.4	4.9	8.15	1782.7	22.1	0.0877389
9	8.45	1904	86.2	0.193073	13	4.08283	7.6	5.8	8.45	1904.6	13.3	0.173302
10	8.65	1977	89.5	0.152501	10	2.71023	5.1	3.9	8.65	1974.7	10.3	0.148102
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2144	97.1	0.286787	26	12.2133	22.8	17.4	9.3	2146.2	25.5	0.269971

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 6.400 4.550 16.200 5.200 12.800 5.850 14.100 6.550 4.200 6.850 6.100 7.350 8.900 8.150 6.900 8.450 4.700 8.650 6.800 9.300 12.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 14 PeakCount: 11 Return to Lane Navigator
3.500 3.800 4.279 4.800 4.550 7.000 4.950 2.000 5.200 5.100 5.317 5.300 5.629 7.700 5.850 5.800 5.987 10.300 6.081 5.700 6.152 2.300 6.187 2.000 6.430 2.300 6.550 2.200 6.631 1.700 6.698 8.000 6.850 1.600 7.297 1.400 7.350 4.700 8.150 1.800 8.450 4.000 8.650 3.500 9.300 7.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 15 PeakCount: 23 Return to Lane Navigator
4.393 8.200 5.166 2.800 5.437 8.100 5.729 16.600 5.932 4.500 6.055 12.800 6.124 10.500 6.191 5.000 6.222 3.400 6.543 4.400 6.626 3.500 6.687 12.500 7.265 2.100 7.827 2.700 8.528 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 16 PeakCount: 15 Return to Lane Navigator
3.500 4.400 4.550 11.400 5.200 10.600 5.850 14.300 6.550 4.900 6.850 6.800 7.350 9.900 8.150 5.200 8.450 8.900 8.650 6.800 9.300 16.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator
4.402 9.100 4.773 5.800 5.158 2.700 5.418 10.200 5.703 0.400 5.922 5.100 6.026 19.000 6.103 12.900 6.160 6.100 6.191 4.000 6.515 3.900 6.575 2.500 6.646 11.800 7.247 2.500 7.813 3.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 18 PeakCount: 15 Return to Lane Navigator
3.500 5.400 4.550 12.000 5.200 9.900 5.850 13.000 6.550 3.600 6.850 5.500 7.350 8.700 8.150 6.400 8.450 7.600 8.650 5.100 9.300 22.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 19 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.