RFE Fish Species Identification Isoelectric Focusing Tools - Pataganian Toothfish Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pataganian Toothfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Dissostichus eleginoides
FDA Seafood List Common Name: Pataganian Toothfish
FDA Seafood List Market Name: Patagonian Toothfish
Unique IEF Gel Code: 19980128gel02
Unique Web Code: pnh01Srr
Tissue Aliquot Code: disseleg A98-001 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	29	3.7	0.118918	6	1.12929	10.8	8.2	4.55	29.8	6	0.105359
2	5.2	120	15.4	0.123857	4	0.917711	8.7	6.7	5.2	119.9	4.4	0.116805
3	5.85	227	29.1	0.0990015	4	0.740302	7.1	5.4	5.85	227.1	4.4	0.0949035
4	6.55	367	47	0.148456	7	1.6431	15.6	12	6.55	366.2	6.7	0.138082
5	6.85	403	51.6	0.0405582	7	0.43834	4.2	3.2	6.85	403.3	6.7	0.037078
6	7.35	470	60.2	0.110872	5	0.945497	9	6.9	7.35	470	5.1	0.105603
7	8.15	595	76.2	0.0840986	12	1.58704	15.1	11.6	8.15	595.2	11.7	0.0766035
8	8.45	651	83.4	0.135142	8	1.88253	17.9	13.7	8.45	650	8.2	0.12941
9	8.65	706	90.4	0.121442	4	0.927525	8.8	6.8	8.65	705.4	4.5	0.117178
10	9.3	765	98	0.0339572	5	0.289231	2.8	2.1	9.3	765.6	4.6	0.0356673

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.602	30	3.9	0.0273332	5	0.238668	1	0.8	4.602	30.1	5.4	0.0249439
2	4.727	47	6	0.0562399	9	0.844559	3.5	2.8	4.727	47.4	8.5	0.0560082
3	4.838	62	8	0.048027	7	0.510751	2.1	1.7	4.838	62.2	6.7	0.0430969
4	5.037	89	11.4	0.285305	13	5.99724	24.7	19.6	5.037	92.3	12.8	0.264646
5	5.283	125	16	0.23295	5	2.02951	8.4	6.6	5.283	124.8	4.9	0.234657
6	5.419	148	19	0.189391	4	1.49667	6.2	4.9	5.419	147.5	4.3	0.194187
7	5.513	164	21.1	0.0271332	5	0.26902	1.1	0.9	5.513	163.1	5.2	0.0293194
8	5.631	184	23.6	0.289083	4	2.08836	8.6	6.8	5.631	184.6	4.2	0.282214
9	5.75	204	26.2	0.398249	4	2.99633	12.3	9.8	5.75	203.9	4.3	0.393714
10	5.832	218	28	0.165308	7	1.97304	8.1	6.4	5.832	218.6	7	0.158297
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.909	233	29.9	0.0424614	4	0.292704	1.2	1	5.909	233	4.3	0.0383373
12	6.146	281	36.1	0.107607	2	0.413009	1.7	1.3	6.146	281.4	2.1	0.112144
13	6.333	319	40.9	0.28091	6	2.28671	9.4	7.5	6.333	318.7	6.1	0.211489
14	6.451	343	44	0.175898	10	2.8635	11.8	9.4	6.451	343.8	9.9	0.162872

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	18	2.3	0.0887654	6	0.897212	8.8	6.7	4.55	18.7	6.2	0.0818877
2	5.2	103	13.3	0.0799738	6	0.742563	7.3	5.6	5.2	104.4	5.5	0.0756002
3	5.85	216	27.8	0.0928362	5	0.747963	7.3	5.6	5.85	215.5	4.6	0.0914908
4	6.55	360	46.4	0.135312	10	2.11671	20.7	15.8	6.55	358.1	9.5	0.125579
5	6.85	395	50.9	0.0371218	6	0.39016	3.8	2.9	6.85	396.1	6.4	0.0343442
6	7.35	463	59.7	0.0916895	5	0.802477	7.9	6	7.35	463.5	5.2	0.0877649
7	8.15	587	75.6	0.0966041	8	1.31247	12.9	9.8	8.15	587.3	8.3	0.0891933
8	8.45	643	82.9	0.117204	9	1.76933	17.3	13.2	8.45	642.2	8.9	0.112795
9	8.65	700	90.2	0.110618	5	0.875171	8.6	6.6	8.65	699.6	4.7	0.105969
10	9.3	760	97.9	0.0576014	6	0.550453	5.4	4.1	9.3	760	5.6	0.0555788

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.07679	4	0.491373	2.2	1.7	4.55	23.8	4.1	0.0672537
2	4.688	41	5.3	0.0412211	13	0.883208	3.9	3.1	4.688	40.6	13.1	0.0380986
3	4.801	55	7.1	0.0310478	7	0.316147	1.4	1.1	4.801	59	7.4	0.0242311
4	5.108	93	12	0.252678	12	5.24414	23	18.3	5.108	94.5	11.8	0.249934
5	5.315	124	16	0.296747	4	2.04178	9	7.1	5.315	123.2	4.2	0.272316
6	5.438	145	18.7	0.173077	4	1.08424	4.8	3.8	5.438	144.8	3.6	0.172192
7	5.532	161	20.8	0.0255318	4	0.171087	0.8	0.6	5.532	160.5	3.8	0.0254494
8	5.643	180	23.2	0.270424	4	1.67202	7.3	5.8	5.643	180.4	3.7	0.254916
9	5.754	199	25.7	0.375625	4	2.43342	10.7	8.5	5.754	199	3.7	0.370262
10	5.836	213	27.5	0.143981	8	1.8436	8.1	6.4	5.836	213.5	7.7	0.135277
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.152	277	35.7	0.0967808	3	0.419596	1.8	1.5	6.152	276.8	2.6	0.0900889
12	6.333	314	40.5	0.223612	5	1.52848	6.7	5.3	6.333	313.7	4.6	0.189232
13	6.441	336	43.4	0.161944	8	2.38799	10.5	8.3	6.441	338.6	8.4	0.161314
14	6.85	394	50.8	0.0385876	8	0.502393	2.2	1.7	6.85	394.6	8.4	0.0336133
15	7.35	464	59.9	0.0323782	9	0.499615	2.2	1.7	7.35	463	9.3	0.0302657
16	8.15	595	76.8	0.00575788	5	0.0455566	0.2	0.2	8.15	594.9	4.8	0.00540595
17	8.45	650	83.9	0.0547767	8	0.718453	3.2	2.5	8.45	649.2	8.1	0.0499621
18	8.65	702	90.6	0.0487714	4	0.342188	1.5	1.2	8.65	702.1	4.1	0.0466165
19	9.3	758	97.8	0.0207036	4	0.133198	0.6	0.5	9.3	758.5	3.8	0.0198249

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.539	19	2.5	0.0173655	7	0.203345	0.8	0.7	4.539	18.7	7	0.0164597
2	4.659	35	4.5	0.0484519	9	0.747893	3.1	2.4	4.659	35.7	8.7	0.0487404
3	4.765	49	6.3	0.0404588	6	0.386721	1.6	1.2	4.765	49.8	6.3	0.0347548
4	4.961	75	9.7	0.299785	10	5.09496	20.9	16.3	4.961	77.8	10	0.286943
5	5.232	112	14.5	0.271547	4	1.96148	8.1	6.3	5.232	111.8	4.1	0.268204
6	5.363	134	17.3	0.15115	5	1.26801	5.2	4.1	5.363	134.2	4.6	0.153929
7	5.452	149	19.2	0.0229587	5	0.20547	0.8	0.7	5.452	148.5	5.2	0.0221076
8	5.584	171	22.1	0.223397	6	2.46048	10.1	7.9	5.584	171.2	6.1	0.2288
9	5.715	193	24.9	0.288108	7	3.7513	15.4	12	5.715	191.8	7.3	0.290836
10	5.804	208	26.8	0.126938	8	1.79974	7.4	5.8	5.804	208.9	7.9	0.127745
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.152	277	35.7	0.04544	15	0.840829	3.5	2.7	6.152	279.8	14.9	0.0317339
12	6.34	315	40.6	0.174545	11	3.05005	12.5	9.8	6.34	313.4	11.4	0.151253
13	6.468	341	44	0.138172	11	2.56628	10.5	8.2	6.468	341.6	11	0.131271

PeakCounter: 13

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	16	2.1	0.100731	5	0.8073	8.8	6.9	4.55	17	4.9	0.0929933
2	5.2	108	14	0.105037	5	0.914495	10	7.8	5.2	108.1	5.1	0.100357
3	5.85	215	27.9	0.0880314	5	0.689129	7.5	5.9	5.85	214.6	4.6	0.0842673
4	6.55	356	46.2	0.116666	6	1.14176	12.5	9.8	6.55	354.7	5.6	0.115601
5	6.85	390	50.6	0.0283357	6	0.267297	2.9	2.3	6.85	390.4	5.9	0.025525
6	7.35	457	59.3	0.0938903	5	0.812042	8.9	7	7.35	457	5	0.0910915
7	8.15	580	75.2	0.0922762	10	1.47281	16.1	12.6	8.15	579.9	10	0.0833422
8	8.45	641	83.1	0.129149	8	1.84258	20.1	15.8	8.45	640	8.3	0.125021
9	8.65	697	90.4	0.115433	5	0.892127	9.7	7.7	8.65	696.7	4.6	0.109266
10	9.3	752	97.5	0.039447	5	0.32137	3.5	2.8	9.3	751.9	4.5	0.0402855

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 76

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.800 5.200 8.700 5.850 7.100 6.550 15.600 6.850 4.200 7.350 9.000 8.150 15.100 8.450 17.900 8.650 8.800 9.300 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.602 1.000 4.727 3.500 4.838 2.100 5.037 24.700 5.283 8.400 5.419 6.200 5.513 1.100 5.631 8.600 5.750 12.300 5.832 8.100 5.909 1.200 6.146 1.700 6.333 9.400 6.451 11.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 8.800 5.200 7.300 5.850 7.300 6.550 20.700 6.850 3.800 7.350 7.900 8.150 12.900 8.450 17.300 8.650 8.600 9.300 5.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 2.200 4.688 3.900 4.801 1.400 5.108 23.000 5.315 9.000 5.438 4.800 5.532 0.800 5.643 7.300 5.754 10.700 5.836 8.100 6.152 1.800 6.333 6.700 6.441 10.500 6.850 2.200 7.350 2.200 8.150 0.200 8.450 3.200 8.650 1.500 9.300 0.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.539 0.800 4.659 3.100 4.765 1.600 4.961 20.900 5.232 8.100 5.363 5.200 5.452 0.800 5.584 10.100 5.715 15.400 5.804 7.400 6.152 3.500 6.340 12.500 6.468 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 13 Return to Lane Navigator
4.550 8.800 5.200 10.000 5.850 7.500 6.550 12.500 6.850 2.900 7.350 8.900 8.150 16.100 8.450 20.100 8.650 9.700 9.300 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pataganian Toothfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pataganian Toothfish ---- Go to Display Gel Lane Navigator.