RFE Fish Species Identification Isoelectric Focusing Tools - Pataganian Toothfish PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Patagonian Toothfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Dissostichus eleginoides
FDA Seafood List Common Name: Patagonian Toothfish
FDA Seafood List Market Name: Patagonian Toothfish
Unique IEF Gel Code: 19980128gel01
Unique Web Code: png01Srr
Tissue Aliquot Code: disseleg A98-001 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	1.9	0.287214	15	7.346714	9.7	7.3	3.5	47.3	15.4	0.269908
2	4.55	385	17.3	0.402561	14	9.21999	12.1	9.2	4.55	385.3	14.4	0.361525
3	5.2	571	25.7	0.420651	13	9.381485	12.3	9.3	5.2	568.2	13.3	0.397845
4	5.85	868	39.1	0.579884	8	8.308581	10.9	8.2	5.85	867.3	8	0.588954
5	6.55	1218	54.9	0.4636	7	5.287374	7	5.2	6.55	1216.8	6.6	0.454662
6	6.85	1297	58.4	0.280761	9	4.042058	5.3	4	6.85	1297.3	9	0.254207
7	7.35	1479	66.6	0.391191	11	7.619337	10	7.6	7.35	1477.9	11.4	0.375785
8	8.15	1787	80.5	0.137068	18	4.104356	5.4	4.1	8.15	1787.3	17.7	0.131014
9	8.45	1897	85.5	0.287821	14	6.442802	8.5	6.4	8.45	1898.3	14.3	0.254499
10	8.65	1969	88.7	0.277068	15	6.542108	8.6	6.5	8.65	1967.2	14.9	0.247688
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2201	99.1	0.500538	10	7.684341	10.1	7.6	9.3	2198.8	9.7	0.445281

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.321	303	13.6	0.579585	18	16.899759	22.3	18.7	4.321	307.5	17.8	0.536159
2	4.528	369	16.6	0.339883	17	9.319345	12.3	10.3	4.528	369.9	17.1	0.307929
3	4.731	425	19.1	0.036507	11	0.779098	1	0.9	4.731	431.4	11	0.040138
4	5.078	519	23.4	0.051634	12	1.218196	1.6	1.4	5.078	518.2	12.2	0.056557
5	5.196	551	24.8	0.308937	11	6.163322	8.1	6.8	5.196	552.1	10.5	0.330343
6	5.3	599	27	0.110614	12	2.29823	3	2.5	5.3	597.2	11.5	0.112355
7	5.393	643	29	0.16812	8	2.521067	3.3	2.8	5.393	643.5	8.3	0.171343
8	5.442	666	30	0.022611	4	0.159809	0.2	0.2	5.442	667.9	4.2	0.021491
9	5.567	725	32.7	0.403194	8	5.862932	7.8	6.5	5.567	726	8.1	0.410513
10	5.626	753	33.9	0.530804	12	11.025992	14.6	12.2	5.626	754.7	11.7	0.530956
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.681	779	35.1	0.331027	17	9.200156	12.2	10.2	5.681	782.3	16.6	0.311964
12	5.828	848	38.2	0.11033	7	1.358273	1.8	1.5	5.828	848.2	7.1	0.108534
13	6.118	989	44.5	0.207662	5	1.926989	2.5	2.1	6.118	989.4	5.3	0.203263
14	6.299	1077	48.5	0.070991	15	1.707769	2.3	1.9	6.299	1079.6	14.8	0.064904
15	6.352	1103	49.7	0.306764	5	2.70286	3.6	3	6.352	1103.9	5.1	0.296446
16	6.446	1149	51.8	0.127139	12	2.483382	3.3	2.8	6.446	1149.9	11.5	0.121816

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	2.2	0.252533	18	7.763851	11.8	9.7	3.5	48.9	18.5	0.23707
2	4.55	374	16.8	0.282144	17	8.668605	13.2	10.8	4.55	371.9	16.9	0.289494
3	5.2	540	24.3	0.242913	17	7.697905	11.7	9.6	5.2	546.9	16.9	0.256361
4	5.85	856	38.5	0.557501	7	7.175277	10.9	9	5.85	856.9	7.2	0.562262
5	6.55	1188	53.4	0.309516	10	5.062567	7.7	6.3	6.55	1190.8	9.6	0.296198
6	6.85	1276	57.4	0.263461	11	4.647749	7.1	5.8	6.85	1276.6	10.9	0.241495
7	7.35	1456	65.5	0.317048	14	7.740604	11.8	9.7	7.35	1453.6	14	0.311137
8	8.15	1770	79.6	0.100743	16	2.822805	4.3	3.5	8.15	1767.3	16.2	0.098472
9	8.45	1885	84.8	0.207534	11	4.040468	6.2	5	8.45	1883.6	11.2	0.203504
10	8.65	1956	87.9	0.18567	10	3.172561	4.8	4	8.65	1955.5	9.8	0.182633
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2197	98.8	0.409088	10	6.881873	10.5	8.6	9.3	2194.6	10	0.38884

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	58	2.6	0.133729	20	5.144327	4.1	3.4	3.5	56	20.4	0.142329
2	4.322	296	13.3	0.573984	17	16.355835	13.2	10.7	4.322	302.1	16.9	0.544524
3	4.55	362	16.3	0.395018	24	14.424127	11.6	9.5	4.55	359.3	23.8	0.341357
4	4.752	416	18.7	0.049605	8	0.631121	0.5	0.4	4.752	422.7	7.8	0.045673
5	4.912	459	20.6	0.034655	10	0.645272	0.5	0.4	4.912	459.8	10.3	0.035176
6	5.14	520	23.4	0.198969	9	2.4874	2	1.6	5.14	518.8	9.5	0.148414
7	5.2	536	24.1	0.352091	12	7.126843	5.7	4.7	5.2	537.6	12	0.333891
8	5.3	583	26.2	0.10663	11	2.296645	1.8	1.5	5.3	583.3	11.4	0.113429
9	5.396	628	28.2	0.144342	12	3.033573	2.4	2	5.396	629.3	11.5	0.148555
10	5.479	667	30	0.011555	7	0.190604	0.2	0.1	5.479	664	6.6	0.016195
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.567	708	31.8	0.35114	10	6.537102	5.3	4.3	5.567	709.9	10	0.369956
12	5.637	741	33.3	0.569801	12	11.82199	9.5	7.7	5.637	737.9	12.5	0.53489
13	5.686	764	34.3	0.371444	18	11.264285	9.1	7.4	5.686	764.7	17.6	0.361309
14	5.85	841	37.8	0.329053	9	5.500989	4.4	3.6	5.85	840.9	9.2	0.335689
15	6.153	985	44.3	0.158187	7	2.104867	1.7	1.4	6.153	982.3	6.5	0.182418
16	6.323	1066	47.9	0.063217	13	1.435838	1.2	0.9	6.323	1063.9	13.4	0.060571
17	6.382	1094	49.2	0.288239	5	2.66724	2.1	1.7	6.382	1095.4	5.4	0.280297
18	6.504	1152	51.8	0.135133	16	3.668612	3	2.4	6.504	1150.7	16.4	0.126587
19	6.55	1174	52.8	0.101194	8	1.257652	1	0.8	6.55	1174	8.4	0.084651
20	6.85	1262	56.7	0.260423	9	3.912179	3.1	2.6	6.85	1263.4	8.9	0.246813
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	1448	65.1	0.343516	11	6.3656	5.1	4.2	7.35	1446.1	11	0.325298
22	8.15	1758	79	0.044837	21	2.722547	2.2	1.8	8.15	1743.1	20.7	0.0743
23	8.45	1879	84.4	0.151589	12	3.046452	2.5	2	8.45	1878.2	11.6	0.14851
24	8.65	1957	88	0.134494	10	2.55404	2.1	1.7	8.65	1953.7	10.3	0.139316
25	9.3	2197	98.7	0.455414	10	7.068269	5.7	4.6	9.3	2193.5	9.6	0.417403

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.342	304	13.7	0.620061	17	17.080587	24.2	19.2	4.342	309.5	16.6	0.578955
2	4.616	382	17.2	0.355899	11	6.727979	9.5	7.6	4.616	382.5	11.1	0.340716
3	4.853	445	20	0.058844	10	1.080781	1.5	1.2	4.853	443.9	9.9	0.061428
4	5.166	528	23.7	0.069501	24	2.676996	3.8	3	5.166	520.2	24.1	0.062598
5	5.257	564	25.3	0.410122	8	5.390677	7.6	6.1	5.257	564.2	7.6	0.399594
6	5.351	608	27.3	0.183569	11	3.147164	4.5	3.5	5.351	606.4	10.5	0.168596
7	5.435	648	29.1	0.220742	7	2.669919	3.8	3	5.435	647.5	6.9	0.219889
8	5.467	663	29.8	0.041195	5	0.506333	0.7	0.6	5.467	665.9	5.2	0.055337
9	5.605	728	32.7	0.465382	7	5.276987	7.5	5.9	5.605	729.5	6.7	0.442303
10	5.667	757	34	0.577463	11	11.097311	15.7	12.5	5.667	759.6	11.3	0.555277
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.728	786	35.3	0.420379	12	7.745469	11	8.7	5.728	786.4	11.9	0.366045
12	5.872	854	38.4	0.127425	7	1.489455	2.1	1.7	5.872	854.3	7.4	0.113812
13	6.145	985	44.2	0.198313	4	1.521439	2.2	1.7	6.145	983.3	4.2	0.206278
14	6.364	1090	49	0.409895	4	2.796661	4	3.1	6.364	1090.6	4	0.396325
15	6.459	1136	51	0.07457	12	1.487988	2.1	1.7	6.459	1135.2	11.6	0.07259

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	59	2.7	0.25341	35	15.237066	20.2	16.1	3.5	60.9	35.2	0.243961
2	4.55	366	16.4	0.36457	15	9.169668	12.2	9.7	4.55	365	15.2	0.340386
3	5.2	537	24.1	0.437251	10	7.935313	10.5	8.4	5.2	536.1	10.5	0.427962
4	5.85	845	38	0.621742	7	7.428285	9.8	7.8	5.85	846.7	6.7	0.626318
5	6.55	1184	53.2	0.445539	6	4.860473	6.4	5.1	6.55	1182.6	6.4	0.42681
6	6.85	1267	56.9	0.307684	9	4.56432	6.1	4.8	6.85	1266.6	9.2	0.280936
7	7.35	1455	65.4	0.379638	9	5.966415	7.9	6.3	7.35	1452.5	9.5	0.354547
8	8.15	1765	79.3	0.148432	14	3.517221	4.7	3.7	8.15	1767.1	13.6	0.145881
9	8.45	1878	84.4	0.25407	11	4.977221	6.6	5.2	8.45	1876.8	11.5	0.244835
10	8.65	1949	87.6	0.198617	10	3.330713	4.4	3.5	8.65	1947.9	9.7	0.193974
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2189	98.3	0.495386	11	8.428452	11.2	8.9	9.3	2186.6	10.6	0.447952

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 89

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 9.700 4.550 12.100 5.200 12.300 5.850 10.900 6.550 7.000 6.850 5.300 7.350 10.000 8.150 5.400 8.450 8.500 8.650 8.600 9.300 10.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 12 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
4.321 22.300 4.528 12.300 4.731 1.000 5.078 1.600 5.196 8.100 5.300 3.000 5.393 3.300 5.442 0.200 5.567 7.800 5.626 14.600 5.681 12.200 5.828 1.800 6.118 2.500 6.299 2.300 6.352 3.600 6.446 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 13 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 16 Return to Lane Navigator
3.500 11.800 4.550 13.200 5.200 11.700 5.850 10.900 6.550 7.700 6.850 7.100 7.350 11.800 8.150 4.300 8.450 6.200 8.650 4.800 9.300 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 14 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 4.100 4.322 13.200 4.550 11.600 4.752 0.500 4.912 0.500 5.140 2.000 5.200 5.700 5.300 1.800 5.396 2.400 5.479 0.200 5.567 5.300 5.637 9.500 5.686 9.100 5.850 4.400 6.153 1.700 6.323 1.200 6.382 2.100 6.504 3.000 6.550 1.000 6.850 3.100 7.350 5.100 8.150 2.200 8.450 2.500 8.650 2.100 9.300 5.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 15 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 25 Return to Lane Navigator
4.342 24.200 4.616 9.500 4.853 1.500 5.166 3.800 5.257 7.600 5.351 4.500 5.435 3.800 5.467 0.700 5.605 7.500 5.667 15.700 5.728 11.000 5.872 2.100 6.145 2.200 6.364 4.000 6.459 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 16 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 15 Return to Lane Navigator
3.500 20.200 4.550 12.200 5.200 10.500 5.850 9.800 6.550 6.400 6.850 6.100 7.350 7.900 8.150 4.700 8.450 6.600 8.650 4.400 9.300 11.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 17 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Patagonian Toothfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Patagonian Toothfish ---- Go to Display Gel Lane Navigator.