RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus macrocephalus
FDA Seafood List Common Name: Pacific Cod
FDA Seafood List Market Name: Cod (Alaska Cod)
Unique IEF Gel Code: 19940912gel02
Unique Web Code: pch01Srr
Tissue Aliquot Code: gadumacr I94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	48	6	0.100797	4	0.736202	6.2	5	4.55	47.9	4.3	0.0963945
2	5.2	140	17.6	0.158432	4	1.05129	8.9	7.2	5.2	140.2	4	0.147776
3	5.85	250	31.4	0.266742	4	1.74545	14.7	11.9	5.85	250.4	3.7	0.266711
4	6.55	387	48.6	0.126381	8	1.62117	13.7	11.1	6.55	385.6	7.5	0.121668
5	6.85	416	52.2	0.0254363	6	0.229247	1.9	1.6	6.85	416.5	5.8	0.022158
6	7.35	478	60	0.108893	5	0.852706	7.2	5.8	7.35	477.9	4.6	0.104875
7	8.15	604	75.8	0.0812668	9	1.21656	10.3	8.3	8.15	604.6	8.6	0.0796048
8	8.45	662	83.1	0.122639	6	1.3212	11.2	9	8.45	661.6	6.2	0.119358
9	8.65	710	89.1	0.0824538	4	0.593423	5	4.1	8.65	709.3	4.1	0.0811811
10	9.3	765	96	0.261588	6	2.47136	20.9	16.9	9.3	764.3	5.5	0.253416

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.404	27	3.4	0.185758	6	1.96449	7.2	5.3	4.404	26.9	6	0.185173
2	4.608	57	7.1	0.321935	18	8.09432	29.6	21.6	4.608	64.6	18	0.253712
3	5.105	130	16.3	0.0227021	18	0.229469	0.8	0.6	5.105	128.2	18	0.0071926
4	5.186	142	17.8	0.0226505	3	0.100386	0.4	0.3	5.186	142	3.4	0.016733
5	5.274	156	19.5	0.62805	3	3.46621	12.7	9.3	5.274	156	3.1	0.623465
6	5.417	179	22.4	0.43585	3	2.33901	8.5	6.3	5.417	179.3	3.1	0.422986
7	5.596	208	26	0.0154041	7	0.173633	0.6	0.5	5.596	209.6	7.1	0.0137851
8	5.745	232	29	0.49054	3	2.94322	10.7	7.9	5.745	231.8	3.4	0.482274
9	5.807	242	30.3	0.281047	6	2.84181	10.4	7.6	5.807	242.4	6.1	0.26267
10	5.926	264	33	0.0715697	10	1.13633	4.1	3	5.926	261.2	9.8	0.0654093
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.018	282	35.3	0.0245072	7	0.185941	0.7	0.5	6.018	285	7.1	0.0146976
12	6.089	296	37	0.2029	3	0.950204	3.5	2.5	6.089	296	2.6	0.202842
13	6.201	318	39.8	0.195832	4	1.37218	5	3.7	6.201	316.8	4.3	0.180693
14	6.283	334	41.8	0.0564003	5	0.463217	1.7	1.2	6.283	335	4.6	0.0566528
15	6.339	345	43.2	0.0268791	4	0.181298	0.7	0.5	6.339	344.3	4.3	0.0240115
16	6.374	352	44.1	0.0182013	1	0.0377493	0.1	0.1	6.374	352	1.4	0.0150545
17	6.41	359	44.9	0.0320866	2	0.118627	0.4	0.3	6.41	358.8	2	0.0333915
18	6.456	368	46.1	0.0122827	4	0.093115	0.3	0.2	6.456	369.1	4.5	0.0116875
19	7.227	464	58.1	0.0245979	7	0.274426	1	0.7	7.227	465.4	6.8	0.0226445
20	7.35	479	59.9	0.0201199	6	0.197614	0.7	0.5	7.35	477.7	6.2	0.0181242
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.452	495	62	0.018446	7	0.222259	0.8	0.6	7.452	497.8	7.4	0.0170025

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	49	6.1	0.122891	4	0.913888	8.3	6.5	4.55	48.8	4.4	0.11608
2	5.2	148	18.6	0.111243	6	1.15141	10.4	8.2	5.2	148	6.1	0.105897
3	5.85	248	31.1	0.247491	4	1.67789	15.2	12	5.85	247.8	3.9	0.245765
4	6.55	386	48.4	0.0938513	8	1.19419	10.8	8.5	6.55	385.5	7.7	0.0877229
5	6.85	420	52.7	0.0179336	5	0.156376	1.4	1.1	6.85	420.4	5.2	0.0171217
6	7.35	480	60.2	0.0884594	4	0.683566	6.2	4.9	7.35	479.8	4.5	0.0865378
7	8.15	604	75.8	0.0756096	8	1.07852	9.7	7.7	8.15	604	8.1	0.0750563
8	8.45	661	82.9	0.119074	6	1.26209	11.4	9	8.45	660.9	6	0.118843
9	8.65	709	89	0.0841755	4	0.617842	5.6	4.4	8.65	708.8	4.3	0.0816333
10	9.3	763	95.7	0.231095	6	2.33712	21.1	16.7	9.3	762.5	5.9	0.223502

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.414	28	3.5	0.0861705	7	1.01817	5	3.9	4.414	27.5	6.6	0.0875392
2	4.597	58	7.3	0.125778	5	0.500997	2.5	1.9	4.597	55.5	5.4	0.0521822
3	4.658	68	8.5	0.172209	18	5.62831	27.7	21.7	4.658	72.7	18.4	0.172127
4	5.09	139	17.4	0.008116	3	0.0400285	0.2	0.2	5.09	139.5	2.8	0.0080442
5	5.2	157	19.6	0.048848	5	0.387643	1.9	1.5	5.2	157.6	4.7	0.0464454
6	5.33	176	22	0.48132	3	2.38164	11.7	9.2	5.33	175.7	2.8	0.487584
7	5.488	199	24.9	0.275659	3	1.53589	7.6	5.9	5.488	199.2	3.2	0.274002
8	5.646	222	27.8	0.0108788	13	0.172083	0.8	0.7	5.646	220.8	12.8	0.0075627
9	5.832	249	31.2	0.0892581	5	0.67579	3.3	2.6	5.832	249	4.7	0.080579
10	5.893	260	32.5	0.142441	3	0.523166	2.6	2	5.893	259.6	3.2	0.0935964
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.98	277	34.7	0.047649	13	1.48436	7.3	5.7	5.98	267.7	12.8	0.0652362
12	6.057	292	36.5	0.0060954	1	0	0	0	6.057	288	1	0
13	6.113	303	37.9	0.0193325	5	0.245673	1.2	0.9	6.113	306.8	5	0.0277013
14	6.159	312	39	0.0617138	3	0.267792	1.3	1	6.159	312.2	2.8	0.0534279
15	6.241	328	41.1	0.0849895	4	0.680995	3.4	2.6	6.241	328.5	4.2	0.0904886
16	6.333	346	43.3	0.0745254	6	0.752568	3.7	2.9	6.333	346.4	5.8	0.0727763
17	6.436	366	45.8	0.0293623	2	0.0822211	0.4	0.3	6.436	365.6	1.9	0.0244255
18	6.497	378	47.3	0.0410422	9	0.666663	3.3	2.6	6.497	379.4	9	0.0418067
19	7.35	482	60.3	0.0660012	6	0.622706	3.1	2.4	7.35	482.3	5.7	0.0616818
20	8.15	609	76.2	0.0283107	10	0.479242	2.4	1.9	8.15	606.2	10	0.0270525
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	666	83.4	0.0653653	6	0.62197	3.1	2.4	8.45	666.3	6.1	0.0577324
22	8.65	711	89	0.0366177	5	0.285366	1.4	1.1	8.65	710.8	4.5	0.035625
23	9.3	765	95.7	0.111999	7	1.23654	6.1	4.8	9.3	764	6.6	0.106234

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.389	26	3.3	0.220028	6	2.26062	11.3	7.9	4.389	25.6	5.8	0.219226
2	4.54	50	6.3	0.177154	4	0.910221	4.6	3.2	4.54	49.6	4.4	0.118029
3	4.583	57	7.1	0.168076	8	2.15676	10.8	7.5	4.583	57.1	7.9	0.1537
4	5.072	135	16.9	0.0184347	2	0.0580369	0.3	0.2	5.072	134.8	1.8	0.018315
5	5.153	148	18.5	0.0193474	5	0.146516	0.7	0.5	5.153	148	4.8	0.0173545
6	5.255	164	20.5	0.624838	3	3.41348	17.1	11.9	5.255	164.7	3.1	0.618145
7	5.418	189	23.7	0.44354	3	2.49089	12.5	8.7	5.418	188.8	3.4	0.415922
8	5.594	216	27	0.0170432	5	0.153773	0.8	0.5	5.594	216.5	5.2	0.0167623
9	5.796	247	30.9	0.34844	5	2.73898	13.7	9.6	5.796	246.9	4.7	0.328469
10	5.895	264	33	0.0358453	9	0.821817	4.1	2.9	5.895	257.5	9.3	0.0496915
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.009	286	35.8	0.0342443	9	0.505508	2.5	1.8	6.009	285.6	8.8	0.0323108
12	6.097	303	37.9	0.0743481	7	0.830925	4.2	2.9	6.097	304.1	6.5	0.0716711
13	6.153	314	39.3	0.127162	3	0.658659	3.3	2.3	6.153	313.5	3.1	0.119632
14	6.221	327	40.9	0.105449	2	0.443665	2.2	1.5	6.221	327.4	2.3	0.108352
15	6.252	333	41.7	0.167867	2	0.677875	3.4	2.4	6.252	333	2.4	0.162566
16	6.334	349	43.7	0.0847395	7	0.979761	4.9	3.4	6.334	348.6	6.9	0.080039
17	6.428	367	45.9	0.0657058	3	0.324141	1.6	1.1	6.428	366.7	3	0.0618875
18	6.495	380	47.6	0.0313087	7	0.367448	1.8	1.3	6.495	378.8	6.6	0.0314188

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	53	6.6	0.122985	5	0.954374	7.6	6.2	4.55	53.2	4.6	0.116711
2	5.2	154	19.3	0.185109	4	1.1483	9.2	7.4	5.2	153.6	3.7	0.176218
3	5.85	259	32.4	0.261553	4	1.799	14.4	11.6	5.85	259.2	3.9	0.259423
4	6.55	393	49.2	0.133941	7	1.67658	13.4	10.8	6.55	392.3	7.3	0.130473
5	6.85	422	52.8	0.0263506	5	0.226109	1.8	1.5	6.85	422.4	5.4	0.0235175
6	7.35	488	61.1	0.108925	5	0.836762	6.7	5.4	7.35	488.2	4.6	0.103241
7	8.15	606	75.8	0.0783241	9	1.20145	9.6	7.8	8.15	606.3	8.9	0.0760373
8	8.45	662	82.9	0.146715	6	1.49734	12	9.7	8.45	661.7	5.9	0.143652
9	8.65	713	89.2	0.0941934	4	0.664497	5.3	4.3	8.65	712.4	4.1	0.0906418
10	9.3	769	96.2	0.230947	6	2.49489	20	16.1	9.3	768.6	6.2	0.226565

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.200 5.200 8.900 5.850 14.700 6.550 13.700 6.850 1.900 7.350 7.200 8.150 10.300 8.450 11.200 8.650 5.000 9.300 20.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.404 7.200 4.608 29.600 5.105 0.800 5.186 0.400 5.274 12.700 5.417 8.500 5.596 0.600 5.745 10.700 5.807 10.400 5.926 4.100 6.018 0.700 6.089 3.500 6.201 5.000 6.283 1.700 6.339 0.700 6.374 0.100 6.410 0.400 6.456 0.300 7.227 1.000 7.350 0.700 7.452 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 21 Return to Lane Navigator
4.550 8.300 5.200 10.400 5.850 15.200 6.550 10.800 6.850 1.400 7.350 6.200 8.150 9.700 8.450 11.400 8.650 5.600 9.300 21.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.414 5.000 4.597 2.500 4.658 27.700 5.090 0.200 5.200 1.900 5.330 11.700 5.488 7.600 5.646 0.800 5.832 3.300 5.893 2.600 5.980 7.300 6.057 0 6.113 1.200 6.159 1.300 6.241 3.400 6.333 3.700 6.436 0.400 6.497 3.300 7.350 3.100 8.150 2.400 8.450 3.100 8.650 1.400 9.300 6.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
4.389 11.300 4.540 4.600 4.583 10.800 5.072 0.300 5.153 0.700 5.255 17.100 5.418 12.500 5.594 0.800 5.796 13.700 5.895 4.100 6.009 2.500 6.097 4.200 6.153 3.300 6.221 2.200 6.252 3.400 6.334 4.900 6.428 1.600 6.495 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 7.600 5.200 9.200 5.850 14.400 6.550 13.400 6.850 1.800 7.350 6.700 8.150 9.600 8.450 12.000 8.650 5.300 9.300 20.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.