RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Golden Redfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes norvegicus
FDA Seafood List Common Name: Golden Redfish
FDA Seafood List Market Name: Perch, Ocean
Unique IEF Gel Code: 19940908gel01
Unique Web Code: opg01Srr
Tissue Aliquot Code: sebanorv H94-003 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c Co-Migrated	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.7	0.209065	11	3.8282	13.6	10.8	3.5	43.4	11	0.195686
2	4.55	193	17	0.277786	5	2.09913	7.4	5.9	4.55	193.2	4.6	0.25909
3	5.2	272	23.9	0.415793	4	3.19278	11.3	9	5.2	272	4.4	0.408018
4	5.85	422	37.1	0.525981	3	2.98682	10.6	8.4	5.85	422	3.1	0.535332
5	6.55	589	51.8	0.389443	4	2.639	9.4	7.4	6.55	588.6	4	0.371588
6	6.85	628	55.2	0.119249	7	1.23855	4.4	3.5	6.85	628.2	6.5	0.107373
7	7.35	721	63.4	0.39671	5	3.13102	11.1	8.8	7.35	720.6	4.5	0.389217
8	8.15	881	77.4	0.0830052	11	1.50684	5.3	4.2	8.15	881	10.8	0.078806
9	8.45	944	83	0.144151	7	1.73244	6.1	4.9	8.45	944.7	6.9	0.14175
10	8.65	986	86.6	0.128303	6	1.19025	4.2	3.4	8.65	985.4	5.6	0.120658
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1108	97.4	0.514479	5	4.63626	16.5	13.1	9.3	1107.8	5.2	0.499398

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.1	130	11.4	0.387052	13	9.04259	37.2	21.4	4.1	133.4	13.1	0.388221
2	4.658	209	18.3	0.435808	5	3.79897	15.6	9	4.658	208.6	4.9	0.438128
3	4.964	246	21.6	0.164829	8	2.30215	9.5	5.5	4.964	248.5	8.5	0.153219
4	5.852	422	37.1	0.543822	3	3.06474	12.6	7.3	5.852	422.3	3.4	0.515585
5	5.953	446	39.2	0.287301	7	2.93523	12.1	7	5.953	445.6	6.7	0.247748
6	6.092	479	42.1	0.218132	3	1.05111	4.3	2.5	6.092	478.8	2.8	0.214887
7	6.155	494	43.4	0.462298	1	1.18252	4.9	2.8	6.155	493.7	1.4	0.47832
8	6.197	504	44.2	0.036412	3	0.181371	0.7	0.4	6.197	504	2.8	0.0363729
9	6.394	551	48.4	0.0227779	5	0.24588	1	0.6	6.394	548.3	4.7	0.0297723
10	6.801	621	54.5	0.150557	2	0.515859	2.1	1.2	6.801	620.8	2	0.148763

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	41	3.6	0.0967044	15	2.35659	4.2	3.5	3.5	43.8	14.8	0.0896083
2	4.115	133	11.7	0.358678	14	8.46452	14.9	12.7	4.115	134.6	13.6	0.351621
3	4.55	198	17.4	0.129379	6	1.21508	2.1	1.8	4.55	197.4	6.1	0.112595
4	4.658	211	18.5	0.415145	4	3.28349	5.8	4.9	4.658	210.9	4.4	0.420913
5	4.958	247	21.7	0.179014	13	3.49593	6.2	5.3	4.958	252.1	13	0.151851
6	5.2	276	24.3	0.270374	6	2.35316	4.1	3.5	5.2	275.5	5.5	0.2411
7	5.85	420	36.9	0.542361	4	3.2232	5.7	4.8	5.85	420.2	3.5	0.516224
8	5.955	445	39.1	0.300327	5	2.46999	4.4	3.7	5.955	444.9	5.3	0.265221
9	6.099	479	42.1	0.21011	3	1.06928	1.9	1.6	6.099	479	3	0.203837
10	6.158	493	43.3	0.461749	2	1.42393	2.5	2.1	6.158	492.7	1.7	0.465237
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.204	504	44.3	0.032195	3	0.196672	0.3	0.3	6.204	504.3	3.5	0.0319442
12	6.411	553	48.6	0.0232794	4	0.221909	0.4	0.3	6.411	550.9	4.5	0.0278487
13	6.55	586	51.5	0.180404	5	1.64513	2.9	2.5	6.55	585.3	5.5	0.169318
14	6.85	626	55	0.221346	3	1.11732	2	1.7	6.85	626	3.1	0.201469
15	7.35	718	63.1	0.337601	4	2.5534	4.5	3.8	7.35	717.5	4.3	0.331242
16	8.15	878	77.2	0.41112	4	2.81415	5	4.2	8.15	879.2	4.4	0.35707
17	8.45	938	82.4	0.133824	6	0.99889	1.8	1.5	8.45	937.2	6.2	0.0915437
18	8.65	981	86.2	0.344519	24	14.4056	25.4	21.6	8.65	977.1	24	0.338653
19	9.3	1113	97.8	0.472217	4	3.42512	6	5.1	9.3	1112.6	4.2	0.455465

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	41	3.6	0.171453	16	4.13758	15.5	12.4	3.5	41.9	16.3	0.143238
2	4.55	199	17.5	0.303699	5	2.56012	9.6	7.7	4.55	199.5	5.1	0.283912
3	5.2	282	24.8	0.398737	5	3.13978	11.7	9.4	5.2	282	4.5	0.393548
4	5.85	425	37.3	0.497024	3	2.90439	10.8	8.7	5.85	425	3.3	0.495777
5	6.55	595	52.3	0.393405	4	2.58349	9.6	7.7	6.55	594.6	3.8	0.379911
6	6.85	633	55.6	0.112817	7	1.214	4.5	3.6	6.85	633.1	6.9	0.0987004
7	7.35	724	63.6	0.395232	4	3.06488	11.4	9.2	7.35	723.5	4.5	0.388193
8	8.15	881	77.4	0.0784582	10	1.35535	5.1	4.1	8.15	881.3	10.2	0.0752072
9	8.45	946	83.1	0.127614	7	1.47355	5.5	4.4	8.45	946.1	6.5	0.126936
10	8.65	987	86.7	0.114452	5	1.02628	3.8	3.1	8.65	986.6	5.2	0.110641
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1116	98.1	0.44629	4	3.31717	12.4	9.9	9.3	1115.1	4.3	0.435445

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.04	123	10.8	0.193639	10	4.40827	23.7	9.5	4.04	127.8	10.2	0.244763
2	4.674	215	18.9	0.4521	4	3.22612	17.3	6.9	4.674	214.7	4	0.458767
3	4.961	251	22	0.195354	10	3.15333	16.9	6.8	4.961	253.5	10	0.178135
4	5.837	423	37.1	0.550696	3	2.92553	15.7	6.3	5.837	423.1	3.1	0.533078
5	5.933	446	39.2	0.28472	5	2.36765	12.7	5.1	5.933	446.2	5.4	0.247977
6	6.069	479	42.1	0.198047	3	0.924334	5	2	6.069	478.8	2.6	0.196874
7	6.131	494	43.4	0.447639	1	1.10638	5.9	2.4	6.131	493.8	1.4	0.45366
8	6.756	622	54.6	0.13983	2	0.492958	2.6	1.1	6.756	622.5	1.9	0.147161

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.8	0.164671	11	3.10986	12.9	10.2	3.5	45.6	11.1	0.158499
2	4.55	200	17.6	0.298064	5	2.21475	9.2	7.3	4.55	200.2	4.6	0.271891
3	5.2	280	24.6	0.440019	4	3.18744	13.2	10.5	5.2	280.1	4.2	0.429957
4	5.85	427	37.5	0.496742	3	2.68467	11.1	8.8	5.85	426.6	3	0.509162
5	6.55	596	52.3	0.354916	4	2.43938	10.1	8	6.55	596.2	4	0.342544
6	6.85	635	55.8	0.0971974	6	0.977172	4.1	3.2	6.85	635	6.5	0.0850595
7	7.35	727	63.8	0.367295	4	2.83816	11.8	9.3	7.35	726.6	4.5	0.356406
8	8.15	884	77.6	0.0740744	10	1.30799	5.4	4.3	8.15	883.7	10.3	0.0718486
9	8.45	948	83.2	0.121163	7	1.43607	6	4.7	8.45	947.7	6.8	0.120002
10	8.65	988	86.7	0.0993668	5	0.917543	3.8	3	8.65	987.7	5.5	0.0942775
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1114	97.8	0.391979	4	2.9744	12.3	9.8	9.3	1113.4	4.5	0.373564

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP CO MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 70

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 13.600 4.550 7.400 5.200 11.300 5.850 10.600 6.550 9.400 6.850 4.400 7.350 11.100 8.150 5.300 8.450 6.100 8.650 4.200 9.300 16.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
4.100 37.200 4.658 15.600 4.964 9.500 5.852 12.600 5.953 12.100 6.092 4.300 6.155 4.900 6.197 0.700 6.394 1.000 6.801 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 10 Return to Lane Navigator
3.500 4.200 4.115 14.900 4.550 2.100 4.658 5.800 4.958 6.200 5.200 4.100 5.850 5.700 5.955 4.400 6.099 1.900 6.158 2.500 6.204 0.300 6.411 0.400 6.550 2.900 6.850 2.000 7.350 4.500 8.150 5.000 8.450 1.800 8.650 25.400 9.300 6.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 19 Return to Lane Navigator
3.500 15.500 4.550 9.600 5.200 11.700 5.850 10.800 6.550 9.600 6.850 4.500 7.350 11.400 8.150 5.100 8.450 5.500 8.650 3.800 9.300 12.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
4.040 23.700 4.674 17.300 4.961 16.900 5.837 15.700 5.933 12.700 6.069 5.000 6.131 5.900 6.756 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 8 Return to Lane Navigator
3.500 12.900 4.550 9.200 5.200 13.200 5.850 11.100 6.550 10.100 6.850 4.100 7.350 11.800 8.150 5.400 8.450 6.000 8.650 3.800 9.300 12.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Golden Redfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Golden Redfish ---- Go to Display Gel Lane Navigator.