RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Milkfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Chanos chanos
FDA Seafood List Common Name: Milkfish
FDA Seafood List Market Name: Milkfish
Unique IEF Gel Code: 19970220gel03
Unique Web Code: mlh01Srr
Tissue Aliquot Code: chanchan I96-003 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	97	11.3	0.0798458	4	0.515167	4	3.1	4.55	97.7	3.8	0.0757342
2	5.2	189	22.1	0.0935855	5	0.768882	5.9	4.6	5.2	189	5	0.0861133
3	5.85	302	35.3	0.247453	3	1.45149	11.2	8.7	5.85	302.4	3.2	0.254207
4	6.55	445	52	0.143182	8	1.98369	15.3	11.9	6.55	443.8	8.4	0.133826
5	6.85	479	56	0.0715411	4	0.498477	3.9	3	6.85	479.7	4.2	0.0670184
6	7.35	549	64.2	0.143937	4	0.967837	7.5	5.8	7.35	548.9	3.9	0.141317
7	8.15	668	78.1	0.128973	8	1.65803	12.8	9.9	8.15	668.1	7.6	0.123019
8	8.45	731	85.5	0.134649	8	1.83593	14.2	11	8.45	729.9	7.8	0.132976
9	8.65	786	91.9	0.132605	5	1.05682	8.2	6.3	8.65	785.7	5	0.118964
10	9.3	828	96.8	0.169856	9	2.19958	17	13.2	9.3	828.3	8.6	0.143929

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.073	26	3	0.0861657	5	0.779673	2	1.8	4.073	25.6	5.2	0.0838904
2	4.197	44	5.2	0.0168921	5	0.123488	0.3	0.3	4.197	43.7	4.5	0.0154256
3	4.598	102	11.9	0.013691	3	0.068882	0.2	0.2	4.598	102	3.3	0.0117382
4	4.757	125	14.6	0.265973	4	1.8941	4.9	4.4	4.757	125	4.1	0.263179
5	4.84	137	16	0.201277	5	1.81297	4.7	4.2	4.84	135.7	5.1	0.199749
6	4.979	157	18.4	0.167642	4	1.10064	2.9	2.5	4.979	156.6	3.6	0.170346
7	5.276	202	23.7	0.168049	5	1.45242	3.8	3.4	5.276	201.6	5	0.164266
8	5.713	277	32.4	0.0191985	10	0.239806	0.6	0.6	5.713	278.3	9.5	0.0142071
9	5.812	294	34.4	0.0159148	4	0.0874635	0.2	0.2	5.812	293.1	3.6	0.0137655
10	5.902	311	36.4	0.1274	3	0.764742	2	1.8	5.902	311	3.3	0.129005
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.966	324	37.9	0.0166066	5	0.126019	0.3	0.3	5.966	324.9	4.6	0.0154785
12	6.069	345	40.4	0.0263475	5	0.244779	0.6	0.6	6.069	345.6	5.2	0.0263536
13	6.163	364	42.6	0.034064	4	0.266642	0.7	0.6	6.163	363.5	4.3	0.0351041
14	6.252	382	44.7	0.112307	2	0.423775	1.1	1	6.252	382.2	2.1	0.112964
15	6.311	394	46.1	0.477581	3	2.14889	5.6	5	6.311	394.3	2.6	0.457788
16	6.41	414	48.5	0.252356	5	1.79287	4.7	4.1	6.41	413.4	4.6	0.219004
17	6.508	434	50.8	0.04293	3	0.219643	0.6	0.5	6.508	433.4	2.9	0.0424009
18	6.548	442	51.8	0.0680059	2	0.294219	0.8	0.7	6.548	442.7	2.4	0.0697501
19	6.6	448	52.5	0.114456	3	0.622639	1.6	1.4	6.6	448.4	3.1	0.114278
20	6.682	457	53.5	0.300307	3	1.58125	4.1	3.7	6.682	457.1	2.9	0.305469
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.745	464	54.3	0.109724	3	0.531619	1.4	1.2	6.745	464.5	2.8	0.106493
22	6.868	478	56	0.351377	2	1.27326	3.3	2.9	6.868	477.4	2	0.364012
23	6.941	488	57.1	0.33905	2	1.21901	3.2	2.8	6.941	488	2	0.342388
24	7.02	499	58.4	0.23686	2	0.970927	2.5	2.2	7.02	498.7	2.2	0.245811
25	7.252	531	62.2	0.135808	7	1.43284	3.7	3.3	7.252	532.7	7.1	0.114343
26	7.36	546	63.9	0.389793	4	2.33926	6.1	5.4	7.36	545.4	3.7	0.356324
27	7.552	575	67.3	0.404108	3	2.48825	6.5	5.7	7.552	575	3.5	0.402288
28	7.838	618	72.4	0.0881501	12	1.84882	4.8	4.3	7.838	623.1	12	0.0868383
29	8.173	670	78.5	0.0750344	6	0.701501	1.8	1.6	8.173	669.5	5.7	0.0699385
30	9.027	806	94.4	0.142697	14	3.6816	9.6	8.5	9.027	808.4	14.5	0.14336
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.412	830	97.2	0.36628	9	5.90409	15.4	13.6	9.412	829.4	9.1	0.364754

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	97	11.3	0.0795335	5	0.591355	4.4	3.3	4.55	97.7	4.6	0.0726565
2	5.2	193	22.5	0.115637	4	0.840476	6.2	4.7	5.2	192.5	4.4	0.10899
3	5.85	303	35.4	0.197211	4	1.43366	10.6	7.9	5.85	302.9	4.1	0.197104
4	6.55	444	51.8	0.156034	8	2.16813	16	12	6.55	442.2	8.4	0.145538
5	6.85	476	55.5	0.0805314	5	0.594124	4.4	3.3	6.85	475.8	4.5	0.0743555
6	7.35	544	63.5	0.167149	4	1.26319	9.3	7	7.35	543.9	4.3	0.164022
7	8.15	666	77.7	0.137838	8	1.75787	13	9.7	8.15	666	7.6	0.13122
8	8.45	732	85.4	0.133365	8	1.97321	14.6	10.9	8.45	730.6	8.4	0.133086
9	8.65	783	91.4	0.149249	5	1.12042	8.3	6.2	8.65	782.9	4.7	0.135042
10	9.3	822	95.9	0.156666	7	1.78245	13.2	9.9	9.3	821.1	6.8	0.14831

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.995	24	2.8	0.0414029	5	0.365122	1.1	1.1	3.995	24.5	4.9	0.0419334
2	4.112	40	4.7	0.00823451	6	0.0822933	0.2	0.2	4.112	42.6	6	0.0077476
3	4.426	83	9.7	0.00340588	2	0.0133627	0	0	4.426	83.8	2.4	0.00320077
4	4.55	100	11.7	0.050606	5	0.430319	1.3	1.2	4.55	100	5	0.0490004
5	4.754	128	15	0.182677	5	1.54461	4.6	4.4	4.754	127.9	4.9	0.178118
6	4.828	138	16.1	0.103889	5	0.961515	2.9	2.8	4.828	137.1	5.1	0.105584
7	4.959	156	18.2	0.0687637	5	0.559665	1.7	1.6	4.959	156.1	4.6	0.0691434
8	5.171	185	21.6	0.0538523	2	0.00879412	0	0	5.171	185.5	2.3	0.00216055
9	5.218	192	22.5	0.0859279	9	1.36597	4.1	3.9	5.218	191.7	9	0.086065
10	5.85	296	34.6	0.0922731	5	0.847091	2.5	2.4	5.85	296.6	5.3	0.0894486
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.931	313	36.6	0.0381215	6	0.393984	1.2	1.1	5.931	313.2	5.8	0.0382666
12	6.127	354	41.4	0.0171407	7	0.214386	0.6	0.6	6.127	349.7	6.9	0.0175513
13	6.179	365	42.7	0.022941	6	0.245696	0.7	0.7	6.179	364.3	6.5	0.0213407
14	6.265	383	44.8	0.0703376	2	0.238906	0.7	0.7	6.265	382.7	1.9	0.0716794
15	6.318	394	46.1	0.382089	3	1.85283	5.5	5.3	6.318	394.6	2.8	0.376048
16	6.423	416	48.7	0.185213	4	1.29781	3.9	3.7	6.423	414.7	4.4	0.165342
17	6.518	436	51	0.0352319	4	0.243913	0.7	0.7	6.518	436.3	3.9	0.0351435
18	6.637	452	52.9	0.0305645	3	0.160234	0.5	0.5	6.637	452.2	3	0.0300799
19	6.72	461	53.9	0.211922	3	0.821072	2.5	2.4	6.72	461	2.6	0.180067
20	6.785	468	54.7	0.0499573	9	0.716883	2.1	2.1	6.785	466.3	9.3	0.0436309
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.886	480	56.1	0.275007	3	1.23786	3.7	3.6	6.886	479.6	2.6	0.26603
22	6.959	490	57.3	0.219652	2	0.880045	2.6	2.5	6.959	489.5	2.2	0.228892
23	7.031	500	58.5	0.158758	3	0.7299	2.2	2.1	7.031	499.7	2.5	0.161585
24	7.249	530	62	0.104831	7	1.19119	3.6	3.4	7.249	532.3	7	0.0961191
25	7.35	544	63.6	0.334972	4	2.39785	7.2	6.9	7.35	543.8	4.3	0.318192
26	7.524	571	66.8	0.311769	4	2.07795	6.2	6	7.524	570.7	3.7	0.313486
27	7.879	626	73.2	0.0669332	18	2.68651	8	7.7	7.879	636.5	18	0.0842072
28	8.15	668	78.1	0.061975	3	0.276926	0.8	0.8	8.15	667.9	3.5	0.0452465
29	8.45	734	85.8	0.0868336	6	0.879024	2.6	2.5	8.45	734	5.8	0.0852548
30	8.65	784	91.7	0.0906597	6	0.741582	2.2	2.1	8.65	784.4	5.9	0.0703387
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.009	805	94.2	0.117963	12	2.23705	6.7	6.4	9.009	802.8	12.1	0.104573
32	9.3	822	96.1	0.223483	10	4.14401	12.4	11.9	9.3	823.1	10.2	0.228535
33	9.488	833	97.4	0.320803	4	1.59011	4.8	4.6	9.488	832.5	3.9	0.230781

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.072	32	3.7	0.0792388	5	0.635796	1.6	1.5	4.072	32.4	4.6	0.0778121
2	4.202	50	5.8	0.0158374	4	0.101032	0.3	0.2	4.202	49.7	3.8	0.015155
3	4.64	111	12.9	0.00810798	4	0.0536151	0.1	0.1	4.64	110.2	3.6	0.00830446
4	4.798	133	15.5	0.298448	4	2.00257	5.1	4.6	4.798	133.3	3.8	0.29617
5	4.884	145	16.9	0.128678	5	1.22835	3.2	2.8	4.884	143.5	5.3	0.131241
6	5.013	163	19	0.121371	5	1.025	2.6	2.3	5.013	162.7	4.6	0.125728
7	5.312	208	24.2	0.117478	7	1.48884	3.8	3.4	5.312	206.2	7.2	0.116379
8	5.782	288	33.5	0.0120564	4	0.082954	0.2	0.2	5.782	288.2	4	0.0115672
9	5.867	303	35.3	0.00946145	3	0.0409764	0.1	0.1	5.867	302.6	2.6	0.00886806
10	5.944	319	37.1	0.122333	3	0.681109	1.8	1.6	5.944	319.5	3.1	0.122116
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.001	331	38.5	0.015955	5	0.125227	0.3	0.3	6.001	331.4	5	0.0142284
12	6.088	349	40.6	0.0336315	5	0.278786	0.7	0.6	6.088	349.7	4.7	0.0333187
13	6.174	367	42.7	0.0416763	5	0.353044	0.9	0.8	6.174	365.8	4.9	0.0407855
14	6.256	384	44.7	0.122404	2	0.496618	1.3	1.1	6.256	383.9	2.4	0.116135
15	6.318	397	46.2	0.458598	3	2.16753	5.6	4.9	6.318	396.9	2.7	0.446993
16	6.424	419	48.8	0.247558	5	1.89401	4.9	4.3	6.424	417.5	5.1	0.210259
17	6.524	440	51.2	0.0489548	5	0.405024	1	0.9	6.524	438.7	5.3	0.042817
18	6.593	450	52.4	0.0601937	2	0.210519	0.5	0.5	6.593	448.9	2.2	0.0549988
19	6.639	455	53	0.1115	3	0.611834	1.6	1.4	6.639	454.7	3.2	0.107794
20	6.712	463	53.9	0.310152	3	1.45097	3.7	3.3	6.712	463.2	2.6	0.312086
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.777	470	54.7	0.102216	2	0.449316	1.2	1	6.777	469.7	2.5	0.10258
22	6.879	482	56.1	0.353649	2	1.27371	3.3	2.9	6.879	481.7	2.1	0.342874
23	6.951	492	57.3	0.295846	2	1.16058	3	2.7	6.951	491.7	2.1	0.306583
24	7.024	502	58.4	0.22084	2	0.948552	2.4	2.2	7.024	502.1	2.4	0.222686
25	7.263	535	62.3	0.121433	8	1.34155	3.4	3.1	7.263	537.2	7.6	0.0995324
26	7.376	551	64.1	0.391674	4	2.55295	6.6	5.8	7.376	550.8	4.1	0.35348
27	7.578	582	67.8	0.412476	4	2.8863	7.4	6.6	7.578	582	4	0.407257
28	7.844	623	72.5	0.115466	12	2.46545	6.3	5.6	7.844	628.4	12.1	0.115418
29	8.169	674	78.5	0.0794815	8	1.04241	2.7	2.4	8.169	674.2	8.1	0.0726581
30	9.028	808	94.1	0.164338	13	3.68327	9.5	8.4	9.028	809.8	13	0.159485
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.275	823	95.8	0.231697	8	3.26558	8.4	7.5	9.275	826.5	8.2	0.223624
32	9.489	836	97.3	0.362179	5	2.51445	6.5	5.7	9.489	835.7	4.5	0.311995

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	97	11.3	0.0619089	4	0.451098	3.4	2.5	4.55	97.1	4.3	0.0586797
2	5.2	189	21.9	0.0820249	5	0.73397	5.6	4.1	5.2	189.3	5.4	0.0767186
3	5.85	303	35.2	0.224169	3	1.35068	10.2	7.5	5.85	302.8	3.4	0.225281
4	6.55	448	52	0.127846	9	1.94785	14.7	10.8	6.55	445.9	9	0.122607
5	6.85	481	55.8	0.0867635	6	0.757785	5.7	4.2	6.85	482.2	5.6	0.0761312
6	7.35	550	63.8	0.171649	4	1.18927	9	6.6	7.35	549.5	3.9	0.172213
7	8.15	672	78	0.119854	8	1.67239	12.7	9.3	8.15	672.6	8.3	0.113267
8	8.45	734	85.2	0.148233	8	2.07364	15.7	11.5	8.45	733.1	8.1	0.143988
9	8.65	786	91.2	0.15408	6	1.36438	10.3	7.6	8.65	786	5.6	0.137627
10	9.3	827	95.9	0.142173	7	1.67052	12.6	9.3	9.3	826.4	6.9	0.136738

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 126

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.000 5.200 5.900 5.850 11.200 6.550 15.300 6.850 3.900 7.350 7.500 8.150 12.800 8.450 14.200 8.650 8.200 9.300 17.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.073 2.000 4.197 0.300 4.598 0.200 4.757 4.900 4.840 4.700 4.979 2.900 5.276 3.800 5.713 0.600 5.812 0.200 5.902 2.000 5.966 0.300 6.069 0.600 6.163 0.700 6.252 1.100 6.311 5.600 6.410 4.700 6.508 0.600 6.548 0.800 6.600 1.600 6.682 4.100 6.745 1.400 6.868 3.300 6.941 3.200 7.020 2.500 7.252 3.700 7.360 6.100 7.552 6.500 7.838 4.800 8.173 1.800 9.027 9.600 9.412 15.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 31 Return to Lane Navigator
4.550 4.400 5.200 6.200 5.850 10.600 6.550 16.000 6.850 4.400 7.350 9.300 8.150 13.000 8.450 14.600 8.650 8.300 9.300 13.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.995 1.100 4.112 0.200 4.426 0 4.550 1.300 4.754 4.600 4.828 2.900 4.959 1.700 5.171 0 5.218 4.100 5.850 2.500 5.931 1.200 6.127 0.600 6.179 0.700 6.265 0.700 6.318 5.500 6.423 3.900 6.518 0.700 6.637 0.500 6.720 2.500 6.785 2.100 6.886 3.700 6.959 2.600 7.031 2.200 7.249 3.600 7.350 7.200 7.524 6.200 7.879 8.000 8.150 0.800 8.450 2.600 8.650 2.200 9.009 6.700 9.300 12.400 9.488 4.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 33 Return to Lane Navigator
4.072 1.600 4.202 0.300 4.640 0.100 4.798 5.100 4.884 3.200 5.013 2.600 5.312 3.800 5.782 0.200 5.867 0.100 5.944 1.800 6.001 0.300 6.088 0.700 6.174 0.900 6.256 1.300 6.318 5.600 6.424 4.900 6.524 1.000 6.593 0.500 6.639 1.600 6.712 3.700 6.777 1.200 6.879 3.300 6.951 3.000 7.024 2.400 7.263 3.400 7.376 6.600 7.578 7.400 7.844 6.300 8.169 2.700 9.028 9.500 9.275 8.400 9.489 6.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 32 Return to Lane Navigator
4.550 3.400 5.200 5.600 5.850 10.200 6.550 14.700 6.850 5.700 7.350 9.000 8.150 12.700 8.450 15.700 8.650 10.300 9.300 12.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Milkfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Milkfish ---- Go to Display Gel Lane Navigator.