RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus synagris
FDA Seafood List Common Name: Lane Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940901gel04
Unique Web Code: lsg01Srr
Tissue Aliquot Code: lutjsyna H94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	39	3.5	0.225647	12	4.5556	24.1	16.3	3.5	40.2	12.1	0.211684
2	4.55	190	16.8	0.0818753	11	1.49003	7.9	5.3	4.55	189.9	10.8	0.0780818
3	5.2	268	23.7	0.0915355	8	1.38404	7.3	4.9	5.2	269	8.4	0.0931588
4	5.85	417	36.9	0.239795	4	1.77341	9.4	6.3	5.85	417	4	0.248492
5	6.55	589	52.1	0.197877	4	1.50126	7.9	5.4	6.55	588.2	4.5	0.189245
6	6.85	626	55.4	0.074761	7	0.84862	4.5	3	6.85	626.4	7.1	0.0671719
7	7.35	720	63.7	0.159923	5	1.33283	7.1	4.8	7.35	719.5	4.9	0.154229
8	8.15	879	77.8	0.0553716	9	0.872497	4.6	3.1	8.15	878.4	8.9	0.0550811
9	8.45	941	83.3	0.0715152	6	0.817874	4.3	2.9	8.45	940.4	6.5	0.071029
10	8.65	982	86.9	0.068477	6	0.680632	3.6	2.4	8.65	982.1	6.1	0.0634421
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.195	1086	96.1	0.0769108	16	2.34387	12.4	8.4	9.195	1090.1	16.2	0.0817141
12	9.3	1106	97.9	0.178659	5	1.29873	6.9	4.6	9.3	1105.7	5	0.147784

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.943	105	9.3	0.292115	7	3.25518	14.2	9.4	3.943	103.8	6.5	0.281512
2	4.526	190	16.8	0.0222301	6	0.186336	0.8	0.5	4.526	187.5	5.9	0.0177633
3	4.669	208	18.4	0.033989	5	0.305789	1.3	0.9	4.669	207.8	5.3	0.0327945
4	4.752	218	19.2	0.0293988	5	0.235691	1	0.7	4.752	217.2	4.7	0.0281179
5	4.916	238	21	0.02266	6	0.224223	1	0.6	4.916	239.3	6.4	0.0198494
6	5.056	255	22.5	0.0205655	2	0.0581256	0.3	0.2	5.056	254.7	2	0.0165004
7	5.163	268	23.7	0.320932	4	2.55207	11.2	7.4	5.163	268.4	4.4	0.324108
8	5.293	293	25.9	0.0896714	4	0.630784	2.8	1.8	5.293	292.7	4.3	0.0824134
9	5.338	303	26.7	0.131658	3	0.5861	2.6	1.7	5.338	302.5	2.5	0.130976
10	5.383	313	27.6	0.391113	4	2.55543	11.2	7.4	5.383	313.1	4	0.361896
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.533	346	30.5	0.0238145	4	0.159117	0.7	0.5	5.533	347	4.3	0.020899
12	5.619	365	32.2	0.0759377	3	0.431119	1.9	1.2	5.619	364.4	3.4	0.071827
13	5.796	404	35.7	0.0513586	7	0.535293	2.3	1.5	5.796	402.2	7.2	0.0416688
14	5.878	423	37.3	0.0755788	4	0.509893	2.2	1.5	5.878	424	4.1	0.0693286
15	5.995	452	39.9	0.361537	3	2.00596	8.8	5.8	5.995	452.8	3.2	0.358931
16	6.068	470	41.5	0.303181	3	1.60005	7	4.6	6.068	469.5	3.2	0.283999
17	6.132	486	42.9	0.0595196	7	0.698797	3.1	2	6.132	485.4	7.4	0.0532606
18	6.161	493	43.5	0.0979108	3	0.396445	1.7	1.1	6.161	493.6	3.1	0.0731691
19	6.467	569	50.2	0.0903557	3	0.498702	2.2	1.4	6.467	569.1	3.3	0.0857108
20	6.516	581	51.3	0.0321733	2	0.13915	0.6	0.4	6.516	580.7	2.4	0.0321552
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.562	591	52.2	0.0686956	3	0.296411	1.3	0.9	6.562	591	2.6	0.0641971
22	6.611	597	52.7	0.0842607	1	0.202492	0.9	0.6	6.611	597.9	1.5	0.0767156
23	6.668	604	53.3	0.202536	6	2.1267	9.3	6.2	6.668	605.3	6.2	0.191991
24	6.814	622	54.9	0.0165147	6	0.122966	0.5	0.4	6.814	625.8	5.8	0.0120178
25	6.896	635	56	0.034873	4	0.232484	1	0.7	6.896	634.4	4.4	0.0301494
26	7.005	655	57.8	0.183736	4	1.25612	5.5	3.6	7.005	655.1	3.8	0.184542
27	7.124	677	59.8	0.0189338	5	0.147054	0.6	0.4	7.124	675.9	4.7	0.0175443
28	7.255	701	61.9	0.0120031	3	0.0678902	0.3	0.2	7.255	701.5	3.4	0.011362
29	8.423	936	82.6	0.0657477	8	0.858669	3.8	2.5	8.423	934.5	7.9	0.061492

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.187922	12	3.78018	22.5	14.3	3.5	34.6	11.8	0.180168
2	4.55	189	16.7	0.0895207	10	1.46811	8.8	5.6	4.55	188.6	9.7	0.0851409
3	5.2	269	23.8	0.104977	8	1.4478	8.6	5.5	5.2	268	7.7	0.105752
4	5.85	407	36	0.302387	4	2.07965	12.4	7.9	5.85	406.6	3.8	0.304868
5	6.55	582	51.5	0.218622	4	1.57918	9.4	6	6.55	581.8	4.2	0.214202
6	6.85	619	54.8	0.0806591	6	0.76251	4.5	2.9	6.85	618.8	6.1	0.0704296
7	7.35	709	62.7	0.156384	5	1.33658	8	5.1	7.35	708.5	4.9	0.153065
8	8.15	862	76.3	0.0539187	10	0.911464	5.4	3.5	8.15	862.1	9.7	0.0530019
9	8.45	935	82.7	0.06954	6	0.780614	4.7	3	8.45	934.4	6.4	0.0685535
10	8.65	978	86.5	0.0704682	5	0.605012	3.6	2.3	8.65	978.2	5	0.0684001
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1106	97.9	0.189423	7	2.02109	12.1	7.7	9.3	1103.7	7.3	0.157184

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.176515	12	3.67268	10.7	8.3	3.5	35.9	12.2	0.170422
2	3.792	78	6.9	0.070046	7	1.11251	3.2	2.5	3.792	81.7	6.8	0.0927865
3	3.899	94	8.3	0.307962	5	3.0492	8.9	6.9	3.899	94	5.5	0.314485
4	4.424	173	15.3	0.0279902	6	0.269542	0.8	0.6	4.424	173.3	5.8	0.0264128
5	4.55	192	17	0.08135	8	1.08899	3.2	2.5	4.55	191.1	7.8	0.0789623
6	4.647	203	18	0.0385636	4	0.208245	0.6	0.5	4.647	202.5	3.9	0.03018
7	4.814	222	19.7	0.022125	4	0.139922	0.4	0.3	4.814	222	4.2	0.0185986
8	4.954	238	21.1	0.0253958	3	0.0723988	0.2	0.2	4.954	238.5	3.4	0.012051
9	5.059	250	22.2	0.33117	4	2.43978	7.1	5.5	5.059	250.2	4.1	0.337747
10	5.2	266	23.6	0.044495	8	0.655846	1.9	1.5	5.2	268.4	7.7	0.0479131
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.237	274	24.3	0.0874202	3	0.334691	1	0.8	5.237	274.5	3.5	0.054052
12	5.284	284	25.2	0.113106	2	0.445776	1.3	1	5.284	284.3	2.2	0.114452
13	5.33	294	26.1	0.442284	3	2.0064	5.9	4.5	5.33	294.5	2.7	0.414265
14	5.483	327	29	0.015162	10	0.204819	0.6	0.5	5.483	323.7	9.7	0.0118562
15	5.576	347	30.8	0.0517048	3	0.286409	0.8	0.6	5.576	346.7	3.2	0.0501288
16	5.692	372	33	0.0471626	6	0.473327	1.4	1.1	5.692	371.3	6.5	0.0412135
17	5.762	387	34.3	0.0550129	10	0.772786	2.3	1.7	5.762	387.3	9.5	0.0458306
18	5.85	406	36	0.214409	3	1.25829	3.7	2.8	5.85	405.8	3.4	0.20989
19	5.978	437	38.7	0.368506	3	2.24115	6.5	5	5.978	437.7	3.5	0.361706
20	6.057	456	40.4	0.287061	4	1.72933	5	3.9	6.057	456.4	3.7	0.263804
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.132	474	42	0.0488381	6	0.499164	1.5	1.1	6.132	473.2	6.4	0.0438242
22	6.161	481	42.6	0.0880942	3	0.384629	1.1	0.9	6.161	481.8	2.6	0.0821069
23	6.5	563	49.9	0.0735791	3	0.347853	1	0.8	6.5	562.2	2.8	0.0708048
24	6.55	575	51	0.100592	4	0.637724	1.9	1.4	6.55	575	3.6	0.0996075
25	6.621	585	51.9	0.0641823	2	0.228712	0.7	0.5	6.621	584.9	2	0.0630692
26	6.679	593	52.6	0.128945	1	0.139307	0.4	0.3	6.679	593.2	1.1	0.0744741
27	6.721	599	53.1	0.206525	6	1.98686	5.8	4.5	6.721	599.3	6.1	0.184013
28	6.85	617	54.7	0.0329735	2	0.118447	0.3	0.3	6.85	616.8	2.2	0.0310222
29	6.906	627	55.6	0.0293831	3	0.13765	0.4	0.3	6.906	625.7	2.5	0.0308923
30	6.929	631	55.9	0.0231782	3	0.115451	0.3	0.3	6.929	630.8	3	0.0218798
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.047	652	57.8	0.201169	3	1.06085	3.1	2.4	7.047	652.6	3.1	0.192882
32	7.176	675	59.8	0.0299895	6	0.255396	0.7	0.6	7.176	673.7	6.2	0.023205
33	7.35	706	62.6	0.128072	5	1.1009	3.2	2.5	7.35	705.2	5.2	0.120508
34	8.15	853	75.6	0.0296145	14	0.526621	1.5	1.2	8.15	854.3	13.9	0.0214194
35	8.45	924	81.9	0.0474388	10	0.843611	2.5	1.9	8.45	927.1	10.4	0.0457325
36	8.523	942	83.5	0.0737086	5	0.664052	1.9	1.5	8.523	942.7	5.5	0.0682605
37	8.65	973	86.3	0.0564031	6	0.564747	1.6	1.3	8.65	972.4	5.7	0.0559501
38	9.3	1101	97.6	0.186581	8	2.19932	6.4	5	9.3	1098.4	7.7	0.161497

PeakCounter: 38

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 20
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 22
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.92	99	8.8	0.321891	7	3.91517	18.7	13.2	3.92	98.2	7	0.315083
2	4.438	178	15.8	0.0146365	4	0.111762	0.5	0.4	4.438	175.6	4.3	0.0145798
3	4.558	196	17.4	0.037766	5	0.311207	1.5	1	4.558	197.1	4.6	0.0385163
4	4.643	206	18.2	0.0320408	4	0.245069	1.2	0.8	4.643	205.7	4.3	0.0319994
5	4.822	227	20.1	0.0210111	6	0.212577	1	0.7	4.822	227	6.1	0.0195434
6	4.949	242	21.4	0.0146289	2	0.0380274	0.2	0.1	4.949	242.5	1.6	0.0130285
7	5.06	255	22.6	0.303502	4	2.45661	11.7	8.3	5.06	255.6	4.5	0.309329
8	5.234	279	24.7	0.0877545	4	0.575159	2.7	1.9	5.234	279.8	3.9	0.0840767
9	5.285	290	25.7	0.125332	3	0.594538	2.8	2	5.285	289.5	2.5	0.133021
10	5.326	299	26.5	0.407157	3	2.21318	10.6	7.5	5.326	299.2	3.2	0.392687
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.464	329	29.1	0.0252325	3	0.117468	0.6	0.4	5.464	329.2	2.8	0.0237048
12	5.57	352	31.2	0.0602475	3	0.357094	1.7	1.2	5.57	351.4	3.5	0.058223
13	5.749	391	34.6	0.0327361	8	0.386119	1.8	1.3	5.749	391.4	8.3	0.0263709
14	5.845	412	36.5	0.0645078	4	0.428666	2	1.4	5.845	413.7	4.4	0.0554995
15	5.961	440	39	0.382622	3	1.95908	9.3	6.6	5.961	440.3	3	0.372879
16	6.035	458	40.6	0.270154	3	1.20929	5.8	4.1	6.035	458.5	2.5	0.26912
17	6.101	474	42	0.0458242	2	0.161828	0.8	0.5	6.101	473.8	2.2	0.0422897
18	6.134	482	42.7	0.0751712	2	0.27649	1.3	0.9	6.134	482.1	2.1	0.0750557
19	6.459	561	49.7	0.0935157	3	0.527799	2.5	1.8	6.459	560.2	3.5	0.0857366
20	6.492	569	50.4	0.0218799	3	0.106272	0.5	0.4	6.492	570.3	2.7	0.0221508
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.538	580	51.4	0.0611061	3	0.257659	1.2	0.9	6.538	579.5	2.5	0.0580637
22	6.617	592	52.4	0.207033	6	2.04719	9.8	6.9	6.617	592.9	6.3	0.183597
23	6.757	611	54.1	0.0202651	1	0.0473228	0.2	0.2	6.757	611.4	1.5	0.0178369
24	6.858	625	55.4	0.0240186	2	0.0711894	0.3	0.2	6.858	625.1	1.7	0.0235287
25	6.974	646	57.2	0.213692	3	1.11615	5.3	3.8	6.974	646.5	3.2	0.199001
26	7.096	668	59.2	0.0261983	3	0.126661	0.6	0.4	7.096	667.4	3.2	0.0220915
27	7.217	690	61.1	0.0102955	2	0.0363817	0.2	0.1	7.217	689.5	2.3	0.00894607
28	8.448	930	82.4	0.0895051	8	1.06131	5.1	3.6	8.448	929.8	7.5	0.0797669

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 21
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 23
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	36	3.2	0.28431	16	7.35191	36.3	26.3	3.5	36.1	16.5	0.251932
2	4.55	198	17.5	0.106799	10	1.73882	8.6	6.2	4.55	197.1	9.5	0.102921
3	5.2	277	24.5	0.130851	8	1.79268	8.9	6.4	5.2	277	7.7	0.131184
4	5.85	420	37.2	0.317313	3	1.90732	9.4	6.8	5.85	420	3.4	0.315841
5	6.55	591	52.3	0.193428	4	1.46946	7.3	5.3	6.55	590.9	4.4	0.189482
6	6.85	630	55.8	0.0669232	7	0.743381	3.7	2.7	6.85	630.4	6.9	0.0611126
7	7.35	722	64	0.134848	5	1.21692	6	4.3	7.35	721.4	5.1	0.134155
8	8.15	876	77.6	0.0466274	10	0.775671	3.8	2.8	8.15	875.9	9.6	0.0457935
9	8.45	937	83	0.0618066	7	0.716594	3.5	2.6	8.45	937.3	6.7	0.0599478
10	8.65	976	86.4	0.0583097	5	0.531646	2.6	1.9	8.65	976	5.2	0.0579379
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1101	97.5	0.143586	8	2.00652	9.9	7.2	9.3	1098.8	8.2	0.13844

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 129

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 24.100 4.550 7.900 5.200 7.300 5.850 9.400 6.550 7.900 6.850 4.500 7.350 7.100 8.150 4.600 8.450 4.300 8.650 3.600 9.195 12.400 9.300 6.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 12 Return to Lane Navigator
3.943 14.200 4.526 0.800 4.669 1.300 4.752 1.000 4.916 1.000 5.056 0.300 5.163 11.200 5.293 2.800 5.338 2.600 5.383 11.200 5.533 0.700 5.619 1.900 5.796 2.300 5.878 2.200 5.995 8.800 6.068 7.000 6.132 3.100 6.161 1.700 6.467 2.200 6.516 0.600 6.562 1.300 6.611 0.900 6.668 9.300 6.814 0.500 6.896 1.000 7.005 5.500 7.124 0.600 7.255 0.300 8.423 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 29 Return to Lane Navigator
3.500 22.500 4.550 8.800 5.200 8.600 5.850 12.400 6.550 9.400 6.850 4.500 7.350 8.000 8.150 5.400 8.450 4.700 8.650 3.600 9.300 12.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
3.500 10.700 3.792 3.200 3.899 8.900 4.424 0.800 4.550 3.200 4.647 0.600 4.814 0.400 4.954 0.200 5.059 7.100 5.200 1.900 5.237 1.000 5.284 1.300 5.330 5.900 5.483 0.600 5.576 0.800 5.692 1.400 5.762 2.300 5.850 3.700 5.978 6.500 6.057 5.000 6.132 1.500 6.161 1.100 6.500 1.000 6.550 1.900 6.621 0.700 6.679 0.400 6.721 5.800 6.850 0.300 6.906 0.400 6.929 0.300 7.047 3.100 7.176 0.700 7.350 3.200 8.150 1.500 8.450 2.500 8.523 1.900 8.650 1.600 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 19 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 21 PeakCount: 38 Return to Lane Navigator
3.920 18.700 4.438 0.500 4.558 1.500 4.643 1.200 4.822 1.000 4.949 0.200 5.060 11.700 5.234 2.700 5.285 2.800 5.326 10.600 5.464 0.600 5.570 1.700 5.749 1.800 5.845 2.000 5.961 9.300 6.035 5.800 6.101 0.800 6.134 1.300 6.459 2.500 6.492 0.500 6.538 1.200 6.617 9.800 6.757 0.200 6.858 0.300 6.974 5.300 7.096 0.600 7.217 0.200 8.448 5.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 20 LANE TYPE: Specimen ACTUAL GEL LANE: 22 PeakCount: 28 Return to Lane Navigator
3.500 36.300 4.550 8.600 5.200 8.900 5.850 9.400 6.550 7.300 6.850 3.700 7.350 6.000 8.150 3.800 8.450 3.500 8.650 2.600 9.300 9.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 21 LANE TYPE: pI Markers ACTUAL GEL LANE: 23 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Lane Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.