Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Kawakawa  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Euthynnus affinis
FDA Seafood List Common Name: Kawakawa
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970430gel02
Unique Web Code: kwg02Srr
Tissue Aliquot Code: euthaffi A97-003 #05p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Co-Migrated
Lane c
Specimen
Lane d
pI Markers
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5813.60.186764175.4685514.910.73.583.317.40.17716
24.5539917.90.23085682.97428.15.84.55398.48.10.208148
35.2581260.26895673.348919.26.65.2581.17.30.258284
45.8588139.50.42986564.927313.59.65.85880.46.40.432623
56.55123355.20.24350183.174858.76.26.551232.47.90.225669
66.85131358.80.16377782.1790164.36.851313.880.152971
77.351495670.22665793.590279.877.351493.79.40.215571
88.15178980.20.0716667141.77514.93.58.151788.514.10.0709396
98.45190585.30.123696112.366546.54.68.451906.311.10.120533
108.65197788.60.10570891.633294.53.28.651977.78.80.104215
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3220398.70.279933125.1593214.110.19.3220011.80.246158
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5853.80.1475184.304756.25.43.587.517.60.137718
24.5541118.40.132274132.7919943.54.55413.313.10.119946
35.07355925.10.20594892.882254.23.65.073559.18.60.188221
45.259526.70.15614192.381173.435.2594.39.40.143234
55.76484537.90.20297462.202633.22.85.764844.76.30.197371
65.8588339.60.30770773.770195.44.75.85884.16.90.30622
76.315111149.80.0592136111.131731.61.46.3151110.611.10.0576207
86.426116552.20.0769562141.836062.62.36.4261163.314.30.072378
96.4621183530.050367380.6078390.90.86.4621186.98.40.040693
106.551226550.10787861.171661.71.56.551226.25.90.111935
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.594123855.50.44633664.447886.45.66.5941238.75.60.444207
126.7151271570.122604102.140533.12.76.7151273.79.70.123988
136.817129958.30.153701112.505833.63.16.8171299.510.60.133569
146.85130858.70.115324110.8461611.21.16.851312.810.70.044411
156.962134860.40.067131490.9742611.41.26.9621347.58.50.0643812
167.055138161.90.052966570.70893410.97.0551381.37.40.0539331
177.131140863.10.4077674.319796.25.47.1311408.47.10.344907
187.201143364.30.0488518112.02242.92.57.2011421110.103925
197.302146965.90.054501970.595540.90.77.3021470.16.60.0510931
207.35148666.60.16400772.008322.92.57.3514866.70.168787
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.402150567.50.022129750.2215670.30.37.40215085.50.022833
227.526155169.60.125921102.1132232.67.5261550.39.60.123667
237.662160171.80.37300774.346136.35.47.66216006.70.364544
248.15178179.90.0546221141.3660121.78.151779140.0551166
258.358186783.70.146772102.362423.42.98.3581866.29.70.137473
268.45190585.40.0924212121.883692.72.48.451905.111.70.0911735
278.535193786.90.0720218151.835672.62.38.5351936.414.60.0711612
288.65198088.80.078193181.100881.61.48.651979.48.30.0747798
298.992096940.113365213.781765.44.78.992096.1210.101814
309.3220298.70.351303136.735719.78.49.32199.212.80.297311
PeakCounter: 30
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.11656125.20.20569382.8029675.55.116560.67.90.201107
25.74983637.60.24551162.717626.85.35.749836.16.40.240638
36.304110449.60.031252130.7389871.91.46.3041103.512.70.0327499
46.4151158520.0652079131.405293.52.86.4151157.513.50.0588898
56.45117552.80.064465870.5902461.51.26.451176.86.80.0492104
66.561122755.10.48232254.4785311.28.86.5611227.25.20.482556
76.676125956.60.117293112.236155.64.46.6761259.910.50.119997
86.776128757.80.163266102.700626.85.36.7761287.29.60.158507
96.848130758.70.049354180.6127321.51.26.8481305.17.60.0455352
106.923133459.90.0707734121.336713.32.66.9231331.711.80.064154
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
117.015136761.40.045582590.7378671.81.47.01513688.60.0483983
127.084139262.50.40304574.0304110.17.97.0841392.66.60.344873
137.159141963.70.035210492.615726.55.17.1591404.58.60.171863
147.259145565.40.072418360.7144971.81.47.2591454.15.90.0688786
157.3611492670.0472751100.8468422.11.77.3611493.7100.0476275
167.494154169.20.124391.944374.93.87.4941540.59.20.118643
177.624158971.40.39373974.5019711.38.87.6241588.76.60.386832
188.33185583.30.16948192.509256.34.98.331854.98.90.158548
198.507192486.40.0538707181.652944.13.28.5071924.518.30.051009
208.982208993.80.050684690.7692711.91.58.9822089.49.10.0474865
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5853.80.203536217.1544417.912.83.588.820.90.192806
24.5539717.80.22708182.925777.35.24.55396.68.10.202868
35.257725.90.27769883.698959.36.65.2575.97.80.267291
45.8588539.70.46969965.1517912.99.25.85884.66.30.46039
56.551225550.2731883.497238.86.26.5512257.90.250918
66.85131058.80.17783392.573336.54.66.851309.38.50.170292
77.351493670.2354793.697139.36.67.351490.99.10.229928
88.15178780.20.0687979151.743374.43.18.151786.515.10.0651388
98.45190585.50.118663112.264515.748.451903.711.30.113043
108.65197588.60.110431101.889924.73.48.651973.810.20.104376
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3219598.50.274298125.2798913.29.49.32192.412.30.242236
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.09655524.90.23975972.8781975.45.096555.46.80.237116
25.69982136.80.27682262.662756.555.6998205.60.269297
36.289110149.40.0450821100.7988431.91.56.2891100.59.90.0457247
46.369113951.10.023292250.2106610.50.46.3691138.64.60.0258555
56.449117752.80.066277190.9613662.31.86.4491174.29.50.0572516
66.564122955.10.50840954.7847511.696.56412285.20.521544
76.69126556.80.110348112.254655.54.26.691261.911.10.114677
86.775128957.80.138552112.779796.85.26.7751289.511.30.138307
96.909133259.80.057633690.9213032.21.76.9091330.99.30.0559493
107.011136961.40.054619470.6935881.71.37.0111367.77.20.0544493
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
117.105140362.90.363694127.7018418.714.57.1051399.7120.36156
127.251145665.30.072216460.7615951.91.47.2511455.26.40.0667468
137.491154469.30.11407481.6385943.17.4911543.48.10.113677
147.61158871.20.37551964.1690810.17.97.611587.56.40.369988
158.324185583.20.16791882.325585.74.48.3241855.18.20.159711
168.514192786.50.0686857222.54016.24.88.5141919.421.50.0666479
178.993209193.80.0770333263.074417.55.88.9932081.4260.0667477
PeakCounter: 17
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5934.20.200466237.4975818.813.73.594.422.60.187102
24.5541418.60.21917582.7931475.14.55413.97.90.198561
35.259026.50.2717583.715729.36.85.2589.580.261296
45.8590140.40.44824865.2012139.55.85901.36.40.457284
56.551225550.24633183.256318.166.551224.88.10.227925
66.85131158.80.173433102.775736.95.16.851310.39.80.159907
77.35149166.90.22931593.5816396.67.351488.99.10.222793
88.15178780.20.0675376151.727294.33.28.151785.6150.0651434
98.45190485.40.121996102.180035.548.451903.610.40.118721
108.65197488.60.106752101.821764.63.38.651972.59.90.10344
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3219598.50.265271135.4160313.69.99.32191.412.90.237408
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK CO SP MK SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 100
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
PeakCount: 30 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 17
LANE TYPE: Specimen
ACTUAL GEL LANE: 19
PeakCount: 17 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 18
LANE TYPE: pI Markers
ACTUAL GEL LANE: 20
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln