RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
White Hake ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Urophycis tenuis
FDA Seafood List Common Name: White Hake
FDA Seafood List Market Name: Hake
Unique IEF Gel Code: 19950831gel04
Unique Web Code: hkh01Srr
Tissue Aliquot Code: uroptenu 001 #5 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	117	13.7	0.0468672	4	0.319266	13.5	7.9	4.55	117.1	4.1	0.0439728
2	5.2	220	25.8	0.0220828	6	0.215348	9.1	5.3	5.2	218.6	6	0.0203515
3	5.85	324	37.9	0.0405371	5	0.356283	15.1	8.8	5.85	323.7	5.2	0.0385774
4	6.55	437	51.2	0.0361031	6	0.372707	15.8	9.2	6.55	436	6.1	0.034728
5	6.85	468	54.8	0.0120395	5	0.0857572	3.6	2.1	6.85	467.9	5	0.0097092
6	7.35	531	62.2	0.0122975	6	0.131735	5.6	3.3	7.35	529.7	6.1	0.0121883
7	8.15	656	76.8	0.0278007	9	0.379715	16.1	9.4	8.15	654.2	8.6	0.0248772
8	8.45	713	83.5	0.0215078	7	0.274922	11.6	6.8	8.45	714.1	7.3	0.0211207
9	8.65	766	89.7	0.0267967	5	0.225528	9.6	5.6	8.65	765.7	5	0.0254102

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.881	11	1.3	0.016325	5	0.142358	1.2	0.9	3.881	11.8	5	0.0160128
2	4.16	54	6.3	0.0043687	6	0.0326315	0.3	0.2	4.16	54.4	5.5	0.0033277
3	4.271	71	8.3	0.0480055	7	0.56696	4.7	3.5	4.271	73.3	7.2	0.0443883
4	4.498	106	12.5	0.13023	11	2.47052	20.5	15.1	4.498	109.1	11.1	0.125213
5	4.758	146	17.2	0.0146895	18	0.550612	4.6	3.4	4.758	130.2	18	0.0172586
6	4.907	169	19.9	0.0065902	3	0.0207679	0.2	0.1	4.907	169.3	2.9	0.0040788
7	5.28	227	26.7	0.085114	3	0.47148	3.9	2.9	5.28	226.9	3.1	0.0857443
8	5.402	247	29	0.207798	4	1.45273	12.1	8.9	5.402	247.1	4.4	0.184537
9	5.482	260	30.6	0.129616	8	2.03702	16.9	12.4	5.482	257.2	8.1	0.141265
10	5.562	273	32.1	0.0632881	6	0.593694	4.9	3.6	5.562	271.6	6.4	0.0527114
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.654	288	33.8	0.0210951	9	0.432085	3.6	2.6	5.654	282.3	8.6	0.0284092
12	5.715	298	35	0.0060552	1	0.0031817	0	0	5.715	298.9	1.4	0.0012543
13	5.832	317	37.3	0.0223605	4	0.145801	1.2	0.9	5.832	317	4	0.0207964
14	5.949	336	39.5	0.153717	3	0.84093	7	5.1	5.949	336.3	3.2	0.146632
15	6.085	358	42.1	0.0576351	6	0.539025	4.5	3.3	6.085	358	5.7	0.0529675
16	6.222	380	44.7	0.0220549	3	0.119508	1	0.7	6.222	380	2.9	0.0229979
17	6.271	388	45.6	0.0142074	3	0.0555396	0.5	0.3	6.271	388.4	2.7	0.0115929
18	6.339	399	46.9	0.0879964	6	0.924873	7.7	5.6	6.339	399.7	6.5	0.0803322
19	6.451	417	49	0.0257257	9	0.385141	3.2	2.3	6.451	412.5	8.8	0.024799
20	6.818	462	54.3	0.0654396	2	0.261825	2.2	1.6	6.818	461.9	2.3	0.0653835

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	113	13.2	0.0522041	4	0.384741	16.6	11.3	4.55	112.6	4.3	0.0508552
2	5.2	210	24.6	0.025813	5	0.225508	9.7	6.6	5.2	208.9	5.4	0.0235761
3	5.85	318	37.3	0.0343856	5	0.315473	13.6	9.3	5.85	317.7	5.5	0.0325638
4	6.55	431	50.5	0.0398698	6	0.380612	16.4	11.2	6.55	430.7	5.7	0.0376339
5	6.85	465	54.5	0.0057588	6	0.0711863	3.1	2.1	6.85	465.2	6.4	0.0062464
6	7.35	523	61.3	0.0144035	6	0.152277	6.6	4.5	7.35	522.6	6.3	0.0137323
7	8.15	650	76.2	0.0216244	9	0.300333	12.9	8.8	8.15	648.8	8.6	0.019751
8	8.45	710	83.2	0.0226627	7	0.276207	11.9	8.1	8.45	709.6	7	0.0223918
9	8.65	752	88.2	0.0194905	7	0.217755	9.4	6.4	8.65	750.8	6.5	0.0187708

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.933	15	1.8	0.0155105	5	0.14381	2.6	1.8	3.933	14.6	5.3	0.0151797
2	4.212	58	6.8	0.0090467	6	0.0918477	1.7	1.2	4.212	56.8	6.1	0.0084579
3	4.355	80	9.4	0.008813	12	0.178138	3.3	2.3	4.355	81.3	12	0.0083451
4	4.55	110	12.9	0.0590118	8	0.804543	14.7	10.3	4.55	111	8.2	0.0555969
5	5.2	210	24.6	0.0070532	6	0.0679598	1.2	0.9	5.2	207.9	5.7	0.006672
6	5.28	223	26.2	0.0169245	4	0.107112	2	1.4	5.28	221.7	3.6	0.0168336
7	5.404	243	28.5	0.0921753	8	1.33197	24.4	17	5.404	243	8	0.0937152
8	5.491	257	30.2	0.0390532	6	0.254283	4.7	3.3	5.491	255.2	5.7	0.0250293
9	5.584	272	31.9	0.0117419	15	0.377339	6.9	4.8	5.584	263.6	14.9	0.014336
10	5.701	291	34.2	0.0035536	1	0	0	0	5.701	288	1	0
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	315	37	0.0147496	7	0.170483	3.1	2.2	5.85	314.3	7	0.0137922
12	6.02	341	40	0.0532146	5	0.451119	8.3	5.8	6.02	339.8	5.4	0.0467595
13	6.151	361	42.4	0.0301336	9	0.412116	7.5	5.3	6.151	360.2	9.3	0.0249749
14	6.288	382	44.8	0.0106927	2	0.0284292	0.5	0.4	6.288	381.4	1.8	0.0091433
15	6.341	390	45.8	0.00711	1	0.0134548	0.2	0.2	6.341	388.6	1.5	0.0050966
16	6.393	398	46.7	0.0454594	6	0.417826	7.6	5.3	6.393	399.2	6.3	0.0372116
17	6.55	422	49.5	0.009279	3	0.0541097	1	0.7	6.55	423.2	3.3	0.009253
18	6.85	454	53.3	0.0448199	2	0.191006	3.5	2.4	6.85	454.4	2.4	0.0444096
19	7.35	514	60.3	0.0080534	6	0.0720691	1.3	0.9	7.35	514.2	6	0.0067901
20	8.45	706	82.9	0.0122968	7	0.149683	2.7	1.9	8.45	707	6.9	0.0121985
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	745	87.4	0.0126373	7	0.15078	2.8	1.9	8.65	745.9	7.1	0.0119963

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.952	14	1.6	0.0186504	5	0.146901	1.3	1	3.952	14.5	4.6	0.0180107
2	4.243	59	6.9	0.0082476	3	0.0378087	0.3	0.2	4.243	58.9	2.9	0.0074168
3	4.34	74	8.7	0.0474675	10	0.772493	6.9	5	4.34	77.9	10.4	0.0420142
4	4.573	110	12.9	0.124774	13	2.65174	23.6	17.3	4.573	114	13.2	0.113026
5	4.896	160	18.8	0.0148875	18	0.282701	2.5	1.8	4.896	142.3	18	0.0088611
6	5.032	181	21.3	0.0059231	2	0.0193637	0.2	0.1	5.032	180.3	2.3	0.0047096
7	5.314	225	26.5	0.0931316	3	0.520523	4.6	3.4	5.314	224.8	3.2	0.0904
8	5.446	246	28.9	0.209839	5	1.6525	14.7	10.8	5.446	246.3	4.5	0.206893
9	5.522	258	30.4	0.130475	6	1.32443	11.8	8.6	5.522	257.4	6	0.124837
10	5.623	274	32.2	0.0572293	7	0.690681	6.1	4.5	5.623	272.5	7	0.0557085
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.724	290	34.1	0.0138059	6	0.189454	1.7	1.2	5.724	287.6	6.2	0.0172723
12	5.818	305	35.9	0.0086565	2	0.0270185	0.2	0.2	5.818	305.3	1.8	0.0082817
13	5.906	319	37.5	0.0212857	3	0.105209	0.9	0.7	5.906	319.7	2.7	0.0221695
14	6.017	337	39.6	0.140387	3	0.770201	6.8	5	6.017	337.5	3.3	0.13005
15	6.146	358	42.1	0.0587682	5	0.463154	4.1	3	6.146	358.3	4.9	0.0536016
16	6.269	378	44.5	0.0336106	3	0.143435	1.3	0.9	6.269	377.5	2.8	0.0292521
17	6.319	386	45.4	0.0196654	2	0.0619297	0.6	0.4	6.319	386.4	2	0.0171914
18	6.368	394	46.4	0.119431	4	0.688687	6.1	4.5	6.368	394.3	4.3	0.0904693
19	6.473	411	48.4	0.0146984	8	0.444393	4	2.9	6.473	402.6	8.1	0.031081
20	6.835	453	53.3	0.0589829	2	0.253398	2.3	1.6	6.835	452.5	2.5	0.0581957

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	107	12.6	0.0411871	6	0.406987	16.1	10	4.55	107.7	5.9	0.0392362
2	5.2	208	24.4	0.025393	5	0.210306	8.3	5.2	5.2	207.8	4.8	0.0249727
3	5.85	309	36.3	0.0366194	4	0.230767	9.1	5.7	5.85	308.7	3.7	0.0353691
4	6.55	429	50.4	0.0443986	6	0.441639	17.5	10.8	6.55	427.9	6.2	0.0404545
5	6.85	459	53.9	0.0115534	8	0.116752	4.6	2.9	6.85	461	7.8	0.0084943
6	7.35	520	61.1	0.0197182	7	0.227469	9	5.6	7.35	519	7.1	0.0181865
7	8.15	644	75.7	0.0232942	8	0.297841	11.8	7.3	8.15	643.3	7.7	0.0218817
8	8.45	703	82.6	0.0291209	8	0.373919	14.8	9.2	8.45	702.4	7.8	0.0269284
9	8.65	756	88.8	0.0256365	5	0.224801	8.9	5.5	8.65	755	5	0.0251272

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 88

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 13.500 5.200 9.100 5.850 15.100 6.550 15.800 6.850 3.600 7.350 5.600 8.150 16.100 8.450 11.600 8.650 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
3.881 1.200 4.160 0.300 4.271 4.700 4.498 20.500 4.758 4.600 4.907 0.200 5.280 3.900 5.402 12.100 5.482 16.900 5.562 4.900 5.654 3.600 5.715 0 5.832 1.200 5.949 7.000 6.085 4.500 6.222 1.000 6.271 0.500 6.339 7.700 6.451 3.200 6.818 2.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 16.600 5.200 9.700 5.850 13.600 6.550 16.400 6.850 3.100 7.350 6.600 8.150 12.900 8.450 11.900 8.650 9.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
3.933 2.600 4.212 1.700 4.355 3.300 4.550 14.700 5.200 1.200 5.280 2.000 5.404 24.400 5.491 4.700 5.584 6.900 5.701 0 5.850 3.100 6.020 8.300 6.151 7.500 6.288 0.500 6.341 0.200 6.393 7.600 6.550 1.000 6.850 3.500 7.350 1.300 8.450 2.700 8.650 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
3.952 1.300 4.243 0.300 4.340 6.900 4.573 23.600 4.896 2.500 5.032 0.200 5.314 4.600 5.446 14.700 5.522 11.800 5.623 6.100 5.724 1.700 5.818 0.200 5.906 0.900 6.017 6.800 6.146 4.100 6.269 1.300 6.319 0.600 6.368 6.100 6.473 4.000 6.835 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 16.100 5.200 8.300 5.850 9.100 6.550 17.500 6.850 4.600 7.350 9.000 8.150 11.800 8.450 14.800 8.650 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: White Hake ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
White Hake ---- Go to Display Gel Lane Navigator.