RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Melanogrammus aeglefinus
FDA Seafood List Common Name: Haddock
FDA Seafood List Market Name: Haddock
Unique IEF Gel Code: 19960118gel10
Unique Web Code: hdh02Srr
Tissue Aliquot Code: melaaegl A96-002 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.7	0.0361835	6	0.347589	11.5	8.3	4.55	20.8	5.7	0.0345658
2	5.2	109	14.2	0.0291934	6	0.286013	9.4	6.8	5.2	109.4	5.9	0.0271753
3	6.55	343	44.8	0.0169022	13	0.353671	11.7	8.5	6.55	341.1	12.9	0.0154274
4	8.15	574	74.9	0.0414603	8	0.553888	18.3	13.2	8.15	574.6	7.7	0.0406777
5	8.45	647	84.5	0.0537488	7	0.693981	22.9	16.6	8.45	646.1	7.4	0.0526444
6	8.65	692	90.3	0.0506602	6	0.509823	16.8	12.2	8.65	691.6	6	0.0481795
7	9.3	748	97.7	0.0201105	9	0.285218	9.4	6.8	9.3	747.9	8.6	0.0186981

PeakCounter: 7

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.658	38	5	0.0593349	13	1.4309	11.5	9.6	4.658	39.7	13.4	0.0600431
2	4.824	61	8	0.0219688	6	0.170057	1.4	1.1	4.824	59.7	5.9	0.0161666
3	4.897	71	9.3	0.0072759	1	0.0005461	0	0	4.897	72.6	0.7	0.000447
4	5.27	125	16.4	0.169029	4	1.19965	9.7	8.1	5.27	125.7	4	0.16811
5	5.375	143	18.7	0.117266	5	0.913766	7.4	6.2	5.375	143.2	4.5	0.113345
6	5.497	164	21.5	0.0177416	10	0.3023	2.4	2	5.497	163.7	10.2	0.0167233
7	5.567	176	23	0.0087537	4	0.0359646	0.3	0.2	5.567	176.4	3.8	0.005361
8	5.649	190	24.9	0.016476	2	0.0641285	0.5	0.4	5.649	189.8	2.5	0.0145835
9	5.713	201	26.3	0.160994	3	0.998269	8	6.7	5.713	201.4	3.4	0.165797
10	5.795	215	28.1	0.108218	4	0.733268	5.9	4.9	5.795	214.9	3.8	0.108773
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.888	231	30.2	0.0470236	7	0.512176	4.1	3.5	5.888	231.2	6.8	0.0427474
12	6.046	258	33.8	0.0104581	5	0.0893215	0.7	0.6	6.046	258.1	5.2	0.0097057
13	6.151	276	36.1	0.0398341	4	0.293738	2.4	2	6.151	277.2	4.2	0.0396278
14	6.238	291	38.1	0.0352739	2	0.187153	1.5	1.3	6.238	292.2	2.4	0.0439569
15	6.285	299	39.1	0.238599	2	0.979125	7.9	6.6	6.285	298.9	2.4	0.234739
16	6.419	322	42.1	0.056022	11	0.941314	7.6	6.3	6.419	318.9	10.6	0.0502338
17	7.035	413	54.1	0.0089731	4	0.0535608	0.4	0.4	7.035	413.5	3.5	0.0085347
18	7.127	426	55.8	0.009446	6	0.092311	0.7	0.6	7.127	425.8	5.9	0.0088472
19	7.24	442	57.9	0.0131747	4	0.0984146	0.8	0.7	7.24	442.8	4	0.0139824
20	7.453	472	61.8	0.0070804	5	0.0618275	0.5	0.4	7.453	471.2	4.9	0.0071231
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.552	486	63.6	0.0150226	3	0.0860775	0.7	0.6	7.552	485.4	3.4	0.014412
22	7.708	508	66.5	0.104012	5	0.908222	7.3	6.1	7.708	508.4	5	0.102561
23	8.986	718	94	0.0731327	18	2.25675	18.2	15.2	8.986	723.4	18	0.0707366

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	27	3.5	0.0322816	6	0.327203	10.6	7.7	4.55	27.3	5.7	0.0324309
2	5.2	119	15.6	0.0294764	6	0.292362	9.5	6.9	5.2	118.9	5.8	0.0286628
3	6.55	348	45.5	0.0199677	11	0.373381	12.1	8.8	6.55	344.7	11.1	0.0189814
4	8.15	569	74.5	0.0316378	13	0.665173	21.6	15.7	8.15	569.1	12.7	0.0296006
5	8.45	636	83.2	0.0458043	8	0.584443	19	13.8	8.45	636.2	7.5	0.0437779
6	8.65	686	89.8	0.0389071	6	0.39535	12.8	9.3	8.65	685.1	6	0.0373871
7	9.3	746	97.6	0.0358234	8	0.444801	14.4	10.5	9.3	745.1	7.6	0.0330311

PeakCounter: 7

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	23	3	0.0069333	4	0.0412228	0.3	0.3	4.55	21.1	4	0.0057751
2	4.691	44	5.8	0.0879965	7	0.816948	6.7	5.5	4.691	41.6	7.5	0.0615282
3	4.798	60	7.8	0.0630198	16	1.76892	14.5	11.8	4.798	56.8	15.5	0.0642275
4	4.852	68	8.9	0.0489476	3	0.0292305	0.2	0.2	4.852	69.2	3.2	0.0052099
5	4.932	80	10.5	0.0348607	11	0.468229	3.8	3.1	4.932	82	10.6	0.0249412
6	5.2	120	15.7	0.005112	6	0.0471923	0.4	0.3	5.2	119.5	6.4	0.0041634
7	5.308	138	18	0.107316	7	1.26143	10.3	8.4	5.308	136.9	6.7	0.105738
8	5.379	150	19.6	0.0560492	8	0.794679	6.5	5.3	5.379	152.6	7.6	0.0587462
9	5.523	174	22.7	0.0078495	6	0.0557365	0.5	0.4	5.523	176.6	5.7	0.0055071
10	5.618	190	24.8	0.0063688	2	0.0156339	0.1	0.1	5.618	190.5	1.8	0.0048795
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.672	199	26	0.003151	10	0.0063836	0.1	0	5.672	200.8	10.3	0.0003513
12	5.762	214	28	0.0655314	9	1.05835	8.7	7.1	5.762	213.4	9.2	0.0648654
13	5.839	227	29.7	0.0424542	3	0.228285	1.9	1.5	5.839	227.1	3.3	0.0390134
14	5.953	246	32.2	0.0182468	6	0.193918	1.6	1.3	5.953	244.5	6.4	0.017095
15	6.138	277	36.2	0.0195364	6	0.202259	1.7	1.3	6.138	274.2	5.8	0.0197562
16	6.221	291	38	0.0254208	4	0.195522	1.6	1.3	6.221	291.4	4.4	0.0249222
17	6.299	304	39.7	0.0226729	5	0.185815	1.5	1.2	6.299	305.4	4.6	0.022925
18	6.341	311	40.7	0.160009	4	1.03203	8.4	6.9	6.341	311.2	3.6	0.162213
19	6.472	333	43.5	0.047424	6	0.532521	4.4	3.6	6.472	332.2	6.3	0.0480134
20	7.297	452	59.1	0.0162238	4	0.119379	1	0.8	7.297	451.6	4.1	0.0163058
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.452	474	62	0.00661	4	0.0523091	0.4	0.3	7.452	473.9	4.2	0.0069982
22	7.565	490	64.1	0.0140791	5	0.11623	1	0.8	7.565	490.2	5	0.0132277
23	7.72	512	66.9	0.0972875	6	0.982824	8	6.6	7.72	511.7	5.8	0.0952603
24	8.15	573	74.9	0.068362	4	0.379496	3.1	2.5	8.15	572.1	3.7	0.0577284
25	8.45	640	83.7	0.0101293	8	0.170261	1.4	1.1	8.45	636.9	8	0.0120362
26	8.65	689	90.1	0.0070689	7	0.111742	0.9	0.7	8.65	685.2	6.8	0.0093132
27	8.957	717	93.7	0.0778699	11	1.35172	11.1	9	8.957	719.4	10.7	0.0713453

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.68	41	5.4	0.0611381	8	0.850431	9	6.7	4.68	41.1	8.5	0.0565561
2	4.799	58	7.6	0.0396197	12	0.730015	7.7	5.7	4.799	57.6	11.5	0.0357621
3	4.863	67	8.8	0.0225094	2	0.0239615	0.3	0.2	4.863	69	2.1	0.0065567
4	4.961	81	10.6	0.0148719	4	0.0677649	0.7	0.5	4.961	82.6	4.4	0.0087413
5	5.336	138	18.1	0.13334	5	1.20801	12.8	9.5	5.336	137.7	5.3	0.129211
6	5.443	156	20.5	0.0900418	5	0.828381	8.8	6.5	5.443	155.1	5.2	0.0899191
7	5.549	174	22.9	0.0121721	3	0.0528156	0.6	0.4	5.549	174.3	2.9	0.0103312
8	5.62	186	24.4	0.0069117	9	0.0932311	1	0.7	5.62	184	8.8	0.0059612
9	5.709	201	26.4	0.0157026	3	0.0657304	0.7	0.5	5.709	199.7	3	0.0123769
10	5.768	211	27.7	0.141469	3	0.880932	9.3	6.9	5.768	211.1	3.4	0.14413
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.845	224	29.4	0.0958207	4	0.666631	7.1	5.2	5.845	224	3.9	0.0969285
12	5.94	240	31.5	0.0399491	6	0.386019	4.1	3	5.94	240.2	5.9	0.0372071
13	6.112	269	35.3	0.0140462	6	0.140013	1.5	1.1	6.112	267.5	5.5	0.0142389
14	6.213	286	37.6	0.0407035	5	0.355892	3.8	2.8	6.213	285.2	5	0.0404095
15	6.295	300	39.4	0.0346637	4	0.293517	3.1	2.3	6.295	301.1	4.3	0.0384237
16	6.331	306	40.2	0.170565	3	0.825478	8.8	6.5	6.331	306.4	2.8	0.166084
17	6.473	330	43.4	0.0506377	7	0.559092	5.9	4.4	6.473	329.3	6.6	0.0474698
18	7.25	443	58.2	0.0111632	4	0.0782462	0.8	0.6	7.25	442.6	4.2	0.0103946
19	7.39	463	60.8	0.0077549	6	0.0763107	0.8	0.6	7.39	465.4	5.9	0.0072455
20	7.523	482	63.3	0.0127516	4	0.0851149	0.9	0.7	7.523	482.4	3.8	0.0126905
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.705	508	66.8	0.0927036	5	0.779667	8.3	6.1	7.705	508	4.8	0.0911759
22	8.985	721	94.7	0.0290929	9	0.384168	4.1	3	8.985	718.4	8.9	0.0242811

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.0249684	5	0.233025	8.8	5.4	4.55	23.7	5.2	0.0251401
2	5.2	112	14.7	0.0267456	6	0.287567	10.8	6.7	5.2	113.1	6.2	0.0261193
3	6.55	342	44.8	0.0140528	10	0.256001	9.6	6	6.55	338.4	9.9	0.0146052
4	8.15	572	74.9	0.0362439	8	0.487264	18.3	11.4	8.15	571.9	8	0.034474
5	8.45	646	84.6	0.0420316	10	0.668417	25.1	15.6	8.45	643.7	9.6	0.0391189
6	8.65	694	90.8	0.0452331	6	0.431329	16.2	10.1	8.65	692.8	5.6	0.0436712
7	9.3	753	98.6	0.0244403	7	0.29824	11.2	7	9.3	751.1	7.4	0.0227283

PeakCounter: 7

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 6.55 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 93

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.500 5.200 9.400 6.550 11.700 8.150 18.300 8.450 22.900 8.650 16.800 9.300 9.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 7 Return to Lane Navigator
4.658 11.500 4.824 1.400 4.897 0 5.270 9.700 5.375 7.400 5.497 2.400 5.567 0.300 5.649 0.500 5.713 8.000 5.795 5.900 5.888 4.100 6.046 0.700 6.151 2.400 6.238 1.500 6.285 7.900 6.419 7.600 7.035 0.400 7.127 0.700 7.240 0.800 7.453 0.500 7.552 0.700 7.708 7.300 8.986 18.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 23 Return to Lane Navigator
4.550 10.600 5.200 9.500 6.550 12.100 8.150 21.600 8.450 19.000 8.650 12.800 9.300 14.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 7 Return to Lane Navigator
4.550 0.300 4.691 6.700 4.798 14.500 4.852 0.200 4.932 3.800 5.200 0.400 5.308 10.300 5.379 6.500 5.523 0.500 5.618 0.100 5.672 0.100 5.762 8.700 5.839 1.900 5.953 1.600 6.138 1.700 6.221 1.600 6.299 1.500 6.341 8.400 6.472 4.400 7.297 1.000 7.452 0.400 7.565 1.000 7.720 8.000 8.150 3.100 8.450 1.400 8.650 0.900 8.957 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.680 9.000 4.799 7.700 4.863 0.300 4.961 0.700 5.336 12.800 5.443 8.800 5.549 0.600 5.620 1.000 5.709 0.700 5.768 9.300 5.845 7.100 5.940 4.100 6.112 1.500 6.213 3.800 6.295 3.100 6.331 8.800 6.473 5.900 7.250 0.800 7.390 0.800 7.523 0.900 7.705 8.300 8.985 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 22 Return to Lane Navigator
4.550 8.800 5.200 10.800 6.550 9.600 8.150 18.300 8.450 25.100 8.650 16.200 9.300 11.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 7 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Haddock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.