RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Melanogrammus aeglefinus
FDA Seafood List Common Name: Haddock
FDA Seafood List Market Name: Haddock
Unique IEF Gel Code: 19930823gel08
Unique Web Code: hdh01Srr
Tissue Aliquot Code: melaaegl 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.6	0.0542965	7	0.668149	6.6	5.4	4.55	38.4	7.2	0.0524825
2	5.2	133	16.7	0.068805	8	0.859156	8.5	6.9	5.2	135.7	7.7	0.0626387
3	5.85	247	31	0.0832261	11	1.5961	15.8	12.9	5.85	251.7	11	0.0817017
4	6.55	392	49.2	0.131379	8	1.74095	17.3	14	6.55	391	7.6	0.128647
5	6.85	426	53.5	0.0196082	8	0.260622	2.6	2.1	6.85	424.1	7.5	0.0195049
6	7.35	486	61.1	0.0733774	5	0.68959	6.8	5.6	7.35	486.5	5.4	0.0723741
7	8.15	606	76.1	0.0857489	8	1.20669	12	9.7	8.15	606.4	8.1	0.0842638
8	8.45	670	84.2	0.0824674	8	1.12521	11.2	9.1	8.45	670.3	7.8	0.0814565
9	8.65	720	90.5	0.0550674	6	0.577043	5.7	4.6	8.65	720.4	6.2	0.0522968
10	9.3	766	96.2	0.137587	6	1.35185	13.4	10.9	9.3	765.6	5.9	0.128269

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.477	27	3.4	0.0237698	5	0.199234	0.6	0.6	4.477	26.3	5.1	0.0219532
2	4.55	38	4.8	0.0303757	2	0.0973341	0.3	0.3	4.55	37.5	2.2	0.0254173
3	4.716	63	7.9	0.211315	13	4.87815	15.8	13.7	4.716	62.2	12.8	0.215576
4	4.888	89	11.2	0.0561178	13	1.47672	4.8	4.1	4.888	83.6	12.5	0.0665822
5	5.2	136	17.1	0.0603431	5	0.524111	1.7	1.5	5.2	135.2	5.1	0.0580037
6	5.293	151	19	0.360638	3	2.08452	6.7	5.8	5.293	151.1	3.3	0.351666
7	5.423	172	21.7	0.312778	3	1.72926	5.6	4.8	5.423	172	3.1	0.311143
8	5.584	198	24.9	0.0105407	3	0.0487573	0.2	0.1	5.584	197.2	2.9	0.0095614
9	5.64	207	26.1	0.0196141	5	0.156114	0.5	0.4	5.64	207	4.9	0.0179243
10	5.776	229	28.8	0.458827	3	2.43592	7.9	6.8	5.776	229.2	2.9	0.469153
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	241	30.4	0.456585	4	3.15782	10.2	8.9	5.85	241.2	4	0.442097
12	5.923	255	32.1	0.202699	6	1.96739	6.4	5.5	5.923	254.9	6.1	0.182958
13	6.049	279	35.1	0.0412494	3	0.223942	0.7	0.6	6.049	278.4	3.2	0.0390511
14	6.242	316	39.8	0.0935615	4	0.621472	2	1.7	6.242	314.4	4.1	0.0847299
15	6.304	328	41.3	0.052386	2	0.202122	0.7	0.6	6.304	328.8	1.7	0.0659552
16	6.341	335	42.2	0.47444	2	1.92533	6.2	5.4	6.341	335.2	2.3	0.473378
17	6.466	359	45.2	0.183199	4	1.33535	4.3	3.7	6.466	358.9	4.3	0.175856
18	6.55	375	47.2	0.0325843	7	0.427659	1.4	1.2	6.55	373.1	7.3	0.0331965
19	6.85	412	51.9	0.0685082	8	0.875384	2.8	2.5	6.85	411.9	8.2	0.0601173
20	6.951	426	53.7	0.050047	6	0.505632	1.6	1.4	6.951	426.4	5.9	0.0481375
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.046	439	55.3	0.0359805	5	0.338757	1.1	0.9	7.046	438.6	5.5	0.0347639
22	7.154	454	57.2	0.0140452	3	0.0799234	0.3	0.2	7.154	455.1	3.1	0.0146065
23	7.35	481	60.6	0.0630455	7	0.681154	2.2	1.9	7.35	482.1	6.5	0.0588944
24	7.542	510	64.2	0.0272885	7	0.269447	0.9	0.8	7.542	508.7	6.9	0.0220268
25	7.628	523	65.9	0.0129371	3	0.0646063	0.2	0.2	7.628	524.7	3.4	0.0106452
26	7.8	549	69.1	0.171005	5	1.36814	4.4	3.8	7.8	549.3	4.6	0.168581
27	8.15	602	75.8	0.0577821	7	0.656071	2.1	1.8	8.15	601.5	6.9	0.0533525
28	8.45	668	84.1	0.0434605	5	0.392251	1.3	1.1	8.45	668.7	5	0.0442431
29	8.65	718	90.4	0.0678782	6	0.692491	2.2	1.9	8.65	717.2	5.7	0.0681682
30	9.07	749	94.3	0.085129	9	1.28683	4.2	3.6	9.07	748.1	8.9	0.0815812
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.3	766	96.5	0.0524428	3	0.258768	0.8	0.7	9.3	766.9	3.4	0.0427259

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.466	23	2.9	0.0334308	6	0.320975	1.2	1	4.466	21.8	6.3	0.0287316
2	4.688	56	7.1	0.208466	11	4.11746	15.8	12.5	4.688	59.6	10.7	0.217263
3	4.863	82	10.4	0.0773391	15	1.71128	6.6	5.2	4.863	79.4	15.2	0.0637004
4	5.173	128	16.2	0.0248448	5	0.178735	0.7	0.5	5.173	126.2	4.8	0.020977
5	5.267	143	18.1	0.372105	4	2.20485	8.4	6.7	5.267	143.4	3.5	0.354101
6	5.393	164	20.7	0.352569	3	1.98367	7.6	6	5.393	163.8	3.3	0.342334
7	5.539	188	23.8	0.0203299	4	0.126841	0.5	0.4	5.539	188.7	3.5	0.0203645
8	5.605	199	25.2	0.0143126	6	0.13399	0.5	0.4	5.605	200	5.6	0.0135017
9	5.762	225	28.4	0.454886	3	2.37736	9.1	7.2	5.762	224.9	3	0.453681
10	5.853	240	30.3	0.30359	4	2.13696	8.2	6.5	5.853	240.5	4	0.303328
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.949	259	32.7	0.0814397	7	0.923033	3.5	2.8	5.949	260.8	6.9	0.0753889
12	6.065	282	35.7	0.0532424	8	0.717591	2.7	2.2	6.065	280.4	8.3	0.0485725
13	6.221	313	39.6	0.144169	5	1.09014	4.2	3.3	6.221	313.1	5.3	0.115885
14	6.323	333	42.1	0.480965	3	2.67399	10.2	8.1	6.323	332.6	3.3	0.460583
15	6.439	356	45	0.260816	7	2.73603	10.5	8.3	6.439	355.5	6.7	0.230765
16	6.841	410	51.8	0.0213932	9	0.315912	1.2	1	6.841	408.8	9.3	0.0190786
17	6.933	422	53.4	0.0277396	4	0.161996	0.6	0.5	6.933	421.6	3.7	0.0244002
18	7.023	434	54.9	0.0195931	3	0.0969614	0.4	0.3	7.023	434	2.9	0.0191194
19	7.128	448	56.6	0.0128825	3	0.0683083	0.3	0.2	7.128	447.9	3	0.0127998
20	7.324	474	59.9	0.0059353	4	0.0337623	0.1	0.1	7.324	473.1	3.7	0.0051843
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.524	504	63.7	0.0161858	5	0.132229	0.5	0.4	7.524	504.2	5.2	0.0143717
22	7.629	520	65.7	0.0107387	3	0.0599383	0.2	0.2	7.629	519.4	3.1	0.0110509
23	7.78	543	68.6	0.236447	4	1.41818	5.4	4.3	7.78	542.8	3.5	0.226244
24	9.052	743	93.9	0.037798	6	0.386251	1.5	1.2	9.052	744.6	5.6	0.0386263

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	36	4.5	0.102171	5	0.836154	6.5	5.2	4.55	36.5	4.9	0.0959994
2	5.2	131	16.5	0.169895	5	1.4441	11.2	9	5.2	131.4	4.8	0.169298
3	5.85	241	30.3	0.241528	5	2.17515	16.9	13.5	5.85	240.5	5.1	0.241022
4	6.55	384	48.3	0.242471	4	1.84712	14.3	11.5	6.55	384.2	4.4	0.235314
5	6.85	413	51.9	0.0507791	11	1.10027	8.5	6.9	6.85	417.8	10.9	0.0570712
6	7.35	477	60	0.128628	4	0.874363	6.8	5.4	7.35	476.6	3.8	0.130737
7	8.15	600	75.5	0.128604	6	1.2842	10	8	8.15	599.9	5.6	0.129774
8	8.45	660	83	0.125524	5	1.19791	9.3	7.5	8.45	660.1	5.4	0.12446
9	8.65	711	89.4	0.0902072	4	0.647637	5	4	8.65	710.5	4.1	0.089491
10	9.3	760	95.6	0.155606	6	1.48648	11.5	9.3	9.3	760.4	5.6	0.149671

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.499	23	2.9	0.0427498	4	0.279919	0.8	0.7	4.499	23.1	3.9	0.040592
2	4.714	54	6.8	0.259605	11	4.79662	14.4	11.8	4.714	55.5	10.9	0.249106
3	4.874	77	9.7	0.111598	16	2.88234	8.7	7.1	4.874	76	16.3	0.0998007
4	5.2	124	15.7	0.0187143	4	0.125944	0.4	0.3	5.2	122.6	4.2	0.0168789
5	5.296	140	17.7	0.288345	4	2.2279	6.7	5.5	5.296	139.7	4.3	0.289444
6	5.417	160	20.2	0.229059	4	1.78229	5.4	4.4	5.417	159.4	4.4	0.23026
7	5.555	183	23.1	0.0095163	3	0.0554589	0.2	0.1	5.555	183.8	3.3	0.0093458
8	5.615	193	24.4	0.0143291	5	0.111669	0.3	0.3	5.615	193.2	4.8	0.0131894
9	5.772	219	27.7	0.391605	3	2.23443	6.7	5.5	5.772	218.3	3.1	0.405103
10	5.85	232	29.3	0.382398	4	2.74815	8.3	6.8	5.85	232.5	4.2	0.367809
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.933	249	31.5	0.202065	6	1.89705	5.7	4.7	5.933	249.6	5.9	0.180619
12	6.045	272	34.4	0.0686311	3	0.375105	1.1	0.9	6.045	272.3	3.2	0.06664
13	6.22	308	38.9	0.123321	5	0.90224	2.7	2.2	6.22	306.2	5.4	0.0946475
14	6.284	321	40.6	0.0579271	2	0.230999	0.7	0.6	6.284	321.9	2	0.0655495
15	6.318	328	41.5	0.476334	2	1.73359	5.2	4.3	6.318	328.4	1.9	0.502377
16	6.44	353	44.6	0.224446	5	2.02164	6.1	5	6.44	352.5	5.4	0.21015
17	6.85	405	51.2	0.03143	11	0.516308	1.6	1.3	6.85	403.1	11.2	0.0260535
18	6.944	417	52.7	0.026953	4	0.151507	0.5	0.4	6.944	418	3.6	0.0235852
19	7.053	431	54.5	0.0229013	3	0.128096	0.4	0.3	7.053	430.9	3.2	0.0223833
20	7.155	444	56.1	0.0111487	3	0.0502962	0.2	0.1	7.155	444.2	2.5	0.0111554
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	469	59.3	0.0157972	3	0.097466	0.3	0.2	7.35	468.6	3.4	0.0162967
22	7.542	499	63.1	0.0300982	7	0.352307	1.1	0.9	7.542	498.4	6.7	0.0295286
23	7.625	512	64.7	0.015909	6	0.135517	0.4	0.3	7.625	512.6	6.2	0.0123837
24	7.785	537	67.9	0.234816	4	1.7463	5.3	4.3	7.785	536.8	4.3	0.226797
25	8.45	656	82.9	0.0262692	12	0.390689	1.2	1	8.45	653	11.8	0.0186115
26	8.65	705	89.1	0.106679	15	2.91297	8.8	7.2	8.65	709.8	14.8	0.111392
27	8.962	729	92.2	0.142317	10	2.33747	7	5.8	8.962	731.2	10.2	0.129577

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.485	18	2.3	0.0284138	5	0.224822	0.7	0.6	4.485	17.4	4.7	0.0271421
2	4.767	60	7.6	0.201429	10	2.32392	7.6	6	4.767	55.7	10.2	0.128135
3	4.888	78	9.9	0.115991	19	4.56829	14.9	11.9	4.888	70.6	19.1	0.135249
4	5.197	124	15.7	0.0120797	4	0.0580018	0.2	0.2	5.197	124.3	3.7	0.0088134
5	5.308	142	18	0.367091	4	2.21589	7.2	5.8	5.308	142.2	3.6	0.351982
6	5.431	162	20.5	0.324257	3	1.96887	6.4	5.1	5.431	161.6	3.4	0.324797
7	5.585	187	23.7	0.02746	7	0.208148	0.7	0.5	5.585	182.2	7.2	0.0163149
8	5.634	195	24.7	0.038078	9	0.541257	1.8	1.4	5.634	195.3	9.1	0.0336856
9	5.776	218	27.6	0.433686	3	2.19592	7.2	5.7	5.776	218.4	2.8	0.441169
10	5.855	231	29.2	0.354777	4	2.34809	7.7	6.1	5.855	231.7	3.8	0.349605
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.935	247	31.3	0.185513	5	1.61984	5.3	4.2	5.935	247.3	5.4	0.168984
12	6.045	269	34.1	0.0593171	13	0.940212	3.1	2.4	6.045	274.9	13.1	0.0404743
13	6.219	304	38.5	0.0929172	6	0.866912	2.8	2.3	6.219	301.4	5.6	0.0878212
14	6.289	318	40.3	0.0479195	5	0.439717	1.4	1.1	6.289	320.1	4.8	0.0520564
15	6.319	324	41	0.413447	2	1.44635	4.7	3.8	6.319	324.1	2.1	0.388529
16	6.434	347	43.9	0.216804	6	2.13789	7	5.6	6.434	347	5.9	0.205446
17	6.854	400	50.6	0.0323694	10	0.53143	1.7	1.4	6.854	400.5	10.1	0.0297342
18	6.952	413	52.3	0.0401605	5	0.322402	1.1	0.8	6.952	413.7	5.4	0.0339112
19	7.043	425	53.8	0.0291827	5	0.238439	0.8	0.6	7.043	424.6	4.6	0.0294066
20	7.164	441	55.8	0.0088745	3	0.0609108	0.2	0.2	7.164	439.7	3.3	0.0104052
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.538	495	62.7	0.0134305	6	0.128322	0.4	0.3	7.538	495.5	6.4	0.0113785
22	7.634	510	64.6	0.0053288	2	0.0272469	0.1	0.1	7.634	511.3	2.4	0.0065117
23	7.793	535	67.7	0.218261	4	1.43936	4.7	3.7	7.793	535.2	3.8	0.215837
24	9.007	730	92.4	0.11646	19	3.76255	12.3	9.8	9.007	723.4	18.8	0.113012

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	26	3.3	0.113936	4	0.855275	6.7	5.4	4.55	26.1	4.5	0.10759
2	5.2	126	16	0.212177	4	1.49966	11.8	9.4	5.2	125.6	4	0.209005
3	5.85	229	29	0.242991	4	1.85828	14.6	11.7	5.85	229.3	4.4	0.23927
4	6.55	366	46.4	0.211826	5	1.93961	15.2	12.2	6.55	365.4	5.5	0.200452
5	6.85	395	50.1	0.0366999	7	0.396943	3.1	2.5	6.85	394.9	7.1	0.0314851
6	7.35	463	58.7	0.109496	4	0.717721	5.6	4.5	7.35	462.7	3.7	0.110009
7	8.15	589	74.7	0.135988	6	1.47067	11.5	9.3	8.15	589.2	6.1	0.135923
8	8.45	649	82.3	0.121156	7	1.42532	11.2	9	8.45	649.2	6.6	0.121829
9	8.65	700	88.7	0.10106	4	0.727535	5.7	4.6	8.65	699.3	4.2	0.0973321
10	9.3	752	95.3	0.182185	6	1.86843	14.6	11.8	9.3	751.9	6	0.176609

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 136

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.600 5.200 8.500 5.850 15.800 6.550 17.300 6.850 2.600 7.350 6.800 8.150 12.000 8.450 11.200 8.650 5.700 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.477 0.600 4.550 0.300 4.716 15.800 4.888 4.800 5.200 1.700 5.293 6.700 5.423 5.600 5.584 0.200 5.640 0.500 5.776 7.900 5.850 10.200 5.923 6.400 6.049 0.700 6.242 2.000 6.304 0.700 6.341 6.200 6.466 4.300 6.550 1.400 6.850 2.800 6.951 1.600 7.046 1.100 7.154 0.300 7.350 2.200 7.542 0.900 7.628 0.200 7.800 4.400 8.150 2.100 8.450 1.300 8.650 2.200 9.070 4.200 9.300 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 31 Return to Lane Navigator
4.466 1.200 4.688 15.800 4.863 6.600 5.173 0.700 5.267 8.400 5.393 7.600 5.539 0.500 5.605 0.500 5.762 9.100 5.853 8.200 5.949 3.500 6.065 2.700 6.221 4.200 6.323 10.200 6.439 10.500 6.841 1.200 6.933 0.600 7.023 0.400 7.128 0.300 7.324 0.100 7.524 0.500 7.629 0.200 7.780 5.400 9.052 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 24 Return to Lane Navigator
4.550 6.500 5.200 11.200 5.850 16.900 6.550 14.300 6.850 8.500 7.350 6.800 8.150 10.000 8.450 9.300 8.650 5.000 9.300 11.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.499 0.800 4.714 14.400 4.874 8.700 5.200 0.400 5.296 6.700 5.417 5.400 5.555 0.200 5.615 0.300 5.772 6.700 5.850 8.300 5.933 5.700 6.045 1.100 6.220 2.700 6.284 0.700 6.318 5.200 6.440 6.100 6.850 1.600 6.944 0.500 7.053 0.400 7.155 0.200 7.350 0.300 7.542 1.100 7.625 0.400 7.785 5.300 8.450 1.200 8.650 8.800 8.962 7.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.485 0.700 4.767 7.600 4.888 14.900 5.197 0.200 5.308 7.200 5.431 6.400 5.585 0.700 5.634 1.800 5.776 7.200 5.855 7.700 5.935 5.300 6.045 3.100 6.219 2.800 6.289 1.400 6.319 4.700 6.434 7.000 6.854 1.700 6.952 1.100 7.043 0.800 7.164 0.200 7.538 0.400 7.634 0.100 7.793 4.700 9.007 12.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 24 Return to Lane Navigator
4.550 6.700 5.200 11.800 5.850 14.600 6.550 15.200 6.850 3.100 7.350 5.600 8.150 11.500 8.450 11.200 8.650 5.700 9.300 14.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Haddock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.