RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Great Barracuda ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sphyraena barracuda
FDA Seafood List Common Name: Great Barracuda
FDA Seafood List Market Name: Barracuda
Unique IEF Gel Code: 19960103gel01
Unique Web Code: gbh01Srr
Tissue Aliquot Code: sphybarr L95-002 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	103	12	0.0503777	3	0.307001	7.1	3.8	4.55	103.1	3.4	0.0503979
2	5.2	195	22.8	0.0460898	4	0.280344	6.5	3.4	5.2	195.2	3.6	0.0442752
3	5.85	302	35.3	0.0423186	3	0.238699	5.6	2.9	5.85	301.7	3.2	0.0423797
4	6.55	415	48.5	0.0254106	8	0.33339	7.8	4.1	6.55	412.2	7.9	0.0239154
5	7.35	512	59.8	0.0209265	3	0.103363	2.4	1.3	7.35	512.2	2.9	0.0197766
6	8.15	636	74.3	0.0479127	7	0.499975	11.6	6.1	8.15	635	6.7	0.0420289
7	8.45	701	81.9	0.049468	5	0.459297	10.7	5.6	8.45	700.6	5.1	0.0510727
8	8.65	749	87.5	0.0449329	4	0.324475	7.6	4	8.65	748.5	4.1	0.0441683
9	9.3	816	95.3	0.167828	6	1.74957	40.7	21.5	9.3	817.5	6.2	0.159189

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.97	21	2.5	0.0686282	7	0.815999	10.3	6.4	3.97	20.9	7.1	0.0651063
2	4.55	104	12.1	0.131806	4	1.06048	13.4	8.3	4.55	103.5	4.4	0.134604
3	5.2	197	23	0.0090361	4	0.0796256	1	0.6	5.2	198.7	4.3	0.0104317
4	5.347	221	25.8	0.0084882	10	0.12916	1.6	1	5.347	216.9	10.1	0.0071924
5	5.701	279	32.6	0.012214	6	0.14558	1.8	1.1	5.701	279	6.4	0.0127955
6	5.817	298	34.8	0.11897	5	1.08934	13.8	8.5	5.817	297.7	5.1	0.121635
7	5.904	312	36.4	0.0733605	7	0.845107	10.7	6.6	5.904	311.8	6.9	0.0686397
8	6.041	334	39	0.0615458	4	0.480899	6.1	3.8	6.041	333.5	4.1	0.0653838
9	6.351	384	44.9	0.0302638	3	0.132022	1.7	1	6.351	384.1	2.6	0.0290937
10	6.457	401	46.8	0.0289037	3	0.127528	1.6	1	6.457	400	2.9	0.0247467
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.513	410	47.9	0.0865654	6	0.720286	9.1	5.6	6.513	409.7	5.6	0.0727551
12	6.682	432	50.5	0.190144	2	0.744823	9.4	5.8	6.682	432.2	2.2	0.188286
13	7.27	503	58.8	0.0087706	4	0.057292	0.7	0.4	7.27	503.5	3.6	0.00888
14	7.701	567	66.2	0.0837976	3	0.482142	6.1	3.8	7.701	567.3	3.2	0.083905
15	8.45	698	81.5	0.022132	6	0.213538	2.7	1.7	8.45	696.8	6.2	0.0194759
16	8.65	749	87.5	0.0312192	8	0.337288	4.3	2.6	8.65	749	7.7	0.0245879
17	9.3	812	94.9	0.0537592	5	0.461295	5.8	3.6	9.3	814.4	4.8	0.0540708

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.971	19	2.2	0.134216	5	1.28568	13.5	7.2	3.971	19.5	5.5	0.132254
2	4.564	106	12.4	0.178999	5	1.50486	15.8	8.4	4.564	106.1	4.7	0.180676
3	5.34	222	25.9	0.0241919	8	0.315298	3.3	1.8	5.34	221.4	8.3	0.0214897
4	5.696	279	32.6	0.0239241	8	0.3045	3.2	1.7	5.696	276.8	7.8	0.0219368
5	5.84	302	35.3	0.125927	5	0.968999	10.2	5.4	5.84	300.4	4.7	0.115167
6	5.921	315	36.8	0.0466323	4	0.295234	3.1	1.6	5.921	315.3	4.3	0.0391016
7	6.045	335	39.1	0.137873	5	1.07076	11.2	6	6.045	335.5	4.8	0.126498
8	6.357	385	45	0.0735028	3	0.418194	4.4	2.3	6.357	384.5	3.2	0.074329
9	6.513	410	47.9	0.160948	4	1.08381	11.4	6.1	6.513	410.3	4.1	0.148506
10	6.683	432	50.5	0.183008	3	0.969458	10.2	5.4	6.683	432.2	3	0.185295
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.272	503	58.8	0.0201396	3	0.0997835	1	0.6	7.272	503.7	2.5	0.0221025
12	7.387	518	60.5	0.0152143	5	0.138165	1.4	0.8	7.387	518	5	0.0155033
13	7.561	545	63.7	0.0236077	4	0.171761	1.8	1	7.561	544.8	4.4	0.0221405
14	7.722	570	66.6	0.135957	4	0.911192	9.6	5.1	7.722	569.7	3.7	0.139543

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	104	12.1	0.0454371	4	0.332351	7.7	4.6	4.55	104.4	4.1	0.0457391
2	5.2	202	23.6	0.0463465	8	0.575731	13.4	8	5.2	199.7	7.6	0.0426362
3	5.85	304	35.5	0.0559556	4	0.397116	9.3	5.5	5.85	303.5	4	0.0557977
4	6.55	416	48.6	0.0260061	8	0.35585	8.3	4.9	6.55	414.4	8.4	0.0239027
5	7.35	512	59.8	0.0168078	5	0.123767	2.9	1.7	7.35	512.2	4.6	0.0153447
6	8.15	636	74.3	0.0397986	7	0.480918	11.2	6.7	8.15	636.3	6.7	0.0403907
7	8.45	703	82.1	0.0526651	8	0.719801	16.8	10	8.45	702.4	7.8	0.0520832
8	8.65	746	87.1	0.0493697	6	0.447946	10.4	6.2	8.65	745.9	5.6	0.0450731
9	9.3	811	94.7	0.103649	5	0.859561	20	11.9	9.3	810.9	4.9	0.0986897

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.94	17	2	0.117754	5	1.10978	12.4	7.1	3.94	17.6	5.3	0.117165
2	4.533	105	12.3	0.152078	5	1.31677	14.8	8.5	4.533	104.4	4.8	0.156332
3	5.319	223	26.1	0.016869	6	0.150438	1.7	1	5.319	222.4	6.1	0.0139075
4	5.633	273	31.9	0.0219947	8	0.382901	4.3	2.5	5.633	277	8.3	0.0259971
5	5.79	298	34.8	0.207561	4	1.30764	14.7	8.4	5.79	298	3.7	0.201055
6	5.88	312	36.4	0.0833552	7	0.913286	10.2	5.9	5.88	312	6.7	0.077468
7	6.011	332	38.8	0.0863786	6	0.897494	10.1	5.8	6.011	331.9	5.9	0.0851795
8	6.346	383	44.7	0.0521195	3	0.279233	3.1	1.8	6.346	383	3	0.0526918
9	6.517	409	47.8	0.0838188	5	0.667474	7.5	4.3	6.517	409.1	5.1	0.0742057
10	6.696	432	50.5	0.160481	2	0.710186	8	4.6	6.696	431.6	2.5	0.162011
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.265	502	58.6	0.018107	5	0.141191	1.6	0.9	7.265	501	4.6	0.0172078
12	7.36	514	60	0.0181935	5	0.179447	2	1.2	7.36	516.1	5.3	0.0190549
13	7.541	542	63.3	0.0174344	6	0.164667	1.8	1.1	7.541	545.1	6.2	0.0149944
14	7.684	564	65.9	0.108484	4	0.702745	7.9	4.5	7.684	564.1	3.7	0.108517

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	111	13	0.0432304	4	0.287455	6.8	3.9	4.55	111	3.6	0.0445873
2	5.2	206	24.1	0.0385781	4	0.273572	6.5	3.7	5.2	204.6	4.2	0.0368834
3	5.85	311	36.3	0.040013	4	0.249862	5.9	3.4	5.85	310.2	3.6	0.038903
4	6.55	412	48.1	0.0289261	11	0.478475	11.4	6.5	6.55	412.7	10.6	0.025458
5	7.35	513	59.9	0.0164188	3	0.0855457	2	1.2	7.35	512.3	3	0.0163224
6	8.15	636	74.3	0.0471191	6	0.529262	12.6	7.2	8.15	635.6	6.4	0.0466822
7	8.45	702	82	0.0745006	6	0.811176	19.3	11.1	8.45	701.9	6.3	0.0729626
8	8.65	751	87.7	0.0606706	4	0.424509	10.1	5.8	8.65	751.4	4.1	0.0587455
9	9.3	814	95.1	0.117981	6	1.06913	25.4	14.6	9.3	813.1	5.7	0.106744

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 72

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.100 5.200 6.500 5.850 5.600 6.550 7.800 7.350 2.400 8.150 11.600 8.450 10.700 8.650 7.600 9.300 40.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 3 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 9 Return to Lane Navigator
3.970 10.300 4.550 13.400 5.200 1.000 5.347 1.600 5.701 1.800 5.817 13.800 5.904 10.700 6.041 6.100 6.351 1.700 6.457 1.600 6.513 9.100 6.682 9.400 7.270 0.700 7.701 6.100 8.450 2.700 8.650 4.300 9.300 5.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 4 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 17 Return to Lane Navigator
3.971 13.500 4.564 15.800 5.340 3.300 5.696 3.200 5.840 10.200 5.921 3.100 6.045 11.200 6.357 4.400 6.513 11.400 6.683 10.200 7.272 1.000 7.387 1.400 7.561 1.800 7.722 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 5 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 14 Return to Lane Navigator
4.550 7.700 5.200 13.400 5.850 9.300 6.550 8.300 7.350 2.900 8.150 11.200 8.450 16.800 8.650 10.400 9.300 20.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 9 Return to Lane Navigator
3.940 12.400 4.533 14.800 5.319 1.700 5.633 4.300 5.790 14.700 5.880 10.200 6.011 10.100 6.346 3.100 6.517 7.500 6.696 8.000 7.265 1.600 7.360 2.000 7.541 1.800 7.684 7.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 7 PeakCount: 14 Return to Lane Navigator
4.550 6.800 5.200 6.500 5.850 5.900 6.550 11.400 7.350 2.000 8.150 12.600 8.450 19.300 8.650 10.100 9.300 25.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Great Barracuda ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Great Barracuda ---- Go to Display Gel Lane Navigator.