RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes ruberrimus
FDA Seafood List Common Name: Yelloweye Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19930817gel06
Unique Web Code: eyh01Srr
Tissue Aliquot Code: sebarube 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	103	12.1	0.0675582	5	0.533237	5.7	4.7	4.55	103.3	4.7	0.0646582
2	5.2	203	23.8	0.144651	4	1.10366	11.8	9.8	5.2	202.4	4.3	0.144567
3	5.85	306	35.9	0.184621	4	1.38892	14.9	12.4	5.85	305.5	4.2	0.188795
4	6.55	439	51.5	0.135676	6	1.4214	15.2	12.7	6.55	438.7	5.9	0.135304
5	6.85	470	55.2	0.0280531	6	0.288169	3.1	2.6	6.85	470	6	0.0272635
6	7.35	531	62.3	0.0724993	5	0.657839	7	5.9	7.35	531.3	5	0.0737714
7	8.15	655	76.9	0.0903235	8	1.21423	13	10.8	8.15	655.5	7.7	0.089035
8	8.45	722	84.7	0.0996074	8	1.38328	14.8	12.3	8.45	721.9	7.7	0.100806
9	8.65	775	91	0.0797144	5	0.637071	6.8	5.7	8.65	774.8	4.7	0.0771623
10	9.3	830	97.4	0.0924287	4	0.711408	7.6	6.3	9.3	829.4	4.2	0.0954813

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.242	56	6.6	0.190895	9	2.9319	11.7	7.1	4.242	57.5	9.3	0.177416
2	4.504	94	11	0.109165	13	2.14249	8.6	5.2	4.504	91.7	13	0.092951
3	4.669	118	13.8	0.266566	5	2.00822	8	4.9	4.669	118.8	5.4	0.210654
4	4.821	140	16.4	0.111758	18	3.86844	15.5	9.4	4.821	136.6	18.3	0.119575
5	4.917	154	18.1	0.0765296	10	0.363152	1.5	0.9	4.917	156	10.1	0.0201956
6	5.035	171	20.1	0.0257741	1	0	0	0	5.035	167	1	0
7	5.2	195	22.9	0.0682727	4	0.467273	1.9	1.1	5.2	194.6	3.9	0.0672125
8	5.85	304	35.7	0.0799779	4	0.537972	2.2	1.3	5.85	304.5	3.8	0.0809136
9	5.918	317	37.2	0.310277	3	1.60269	6.4	3.9	5.918	317	2.9	0.310494
10	5.976	328	38.5	0.228951	6	2.26868	9.1	5.5	5.976	329.8	6.4	0.199205
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.124	356	41.8	0.162538	4	1.15992	4.6	2.8	6.124	356.5	4.2	0.155721
12	6.213	373	43.8	0.219072	4	1.19349	4.8	2.9	6.213	372.3	4	0.16867
13	6.355	400	46.9	0.0613484	4	0.469249	1.9	1.1	6.355	399.8	4.5	0.0594832
14	6.487	425	49.9	0.0551765	5	0.185052	0.7	0.5	6.487	425	5.2	0.0199111
15	6.513	430	50.5	0.0681004	12	1.21784	4.9	3	6.513	432.5	12.1	0.0568617
16	6.55	437	51.3	0.0633195	5	0.162925	0.7	0.4	6.55	439.4	4.8	0.0191253
17	6.85	467	54.8	0.01395	4	0.0925714	0.4	0.2	6.85	467.2	3.8	0.0138331
18	7.386	534	62.7	0.114728	10	1.84728	7.4	4.5	7.386	534.4	9.9	0.105194
19	7.67	578	67.8	0.0931614	3	0.576655	2.3	1.4	7.67	578.3	3.5	0.0935529
20	8.45	724	85	0.0502139	6	0.52	2.1	1.3	8.45	723.5	5.9	0.0499393
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	775	91	0.0445393	5	0.350402	1.4	0.9	8.65	774.6	4.7	0.0423238
22	9.3	829	97.3	0.102614	6	1.01352	4.1	2.5	9.3	829.2	5.9	0.0969427

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.22	51	6	0.196833	9	3.00892	11.4	8.3	4.22	51.6	8.7	0.1952
2	4.485	89	10.4	0.192305	9	2.97051	11.3	8.2	4.485	91.7	9	0.186652
3	4.687	118	13.8	0.383138	6	3.75788	14.3	10.3	4.687	117.9	5.9	0.359637
4	4.862	143	16.8	0.113691	18	3.47125	13.2	9.5	4.862	141	17.6	0.110985
5	5.05	170	20	0.0151475	1	0	0	0	5.05	167	1	0
6	5.937	318	37.3	0.388696	4	2.43673	9.3	6.7	5.937	317.7	3.6	0.386668
7	5.985	327	38.4	0.279859	3	1.04005	3.9	2.9	5.985	326.6	3	0.197134
8	6.022	334	39.2	0.152157	6	1.74526	6.6	4.8	6.022	332	6.2	0.159066
9	6.127	354	41.5	0.202874	3	1.12587	4.3	3.1	6.127	354.4	3.2	0.197849
10	6.207	369	43.3	0.329555	2	1.30807	5	3.6	6.207	368.7	2.2	0.32872
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.333	393	46.1	0.100874	6	1.04871	4	2.9	6.333	393.3	6	0.0990581
12	6.455	416	48.8	0.077761	3	0.0797527	0.3	0.2	6.455	414.8	2.8	0.0162329
13	6.487	422	49.5	0.0854824	8	1.12924	4.3	3.1	6.487	420.8	8.3	0.0767311
14	7.391	532	62.4	0.166075	9	2.34757	8.9	6.4	7.391	530.7	8.5	0.155674
15	7.679	577	67.7	0.150681	3	0.866557	3.3	2.4	7.679	577.1	3.2	0.150796

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	94	11.1	0.0664372	5	0.610133	6	4.7	4.55	94.4	5.5	0.0630617
2	5.2	186	21.9	0.160595	4	1.04748	10.4	8.1	5.2	186	3.7	0.158773
3	5.85	297	35	0.183984	4	1.37846	13.7	10.7	5.85	297.1	4.2	0.187159
4	6.55	429	50.5	0.150134	6	1.46604	14.5	11.3	6.55	428.4	5.7	0.144059
5	6.85	457	53.8	0.0346052	6	0.346647	3.4	2.7	6.85	458.4	5.7	0.0343223
6	7.35	521	61.4	0.0887197	5	0.81421	8.1	6.3	7.35	521.8	5.3	0.0861918
7	8.15	646	76.1	0.112912	7	1.27486	12.6	9.9	8.15	646	6.6	0.109697
8	8.45	709	83.5	0.115183	6	1.23311	12.2	9.5	8.45	709.6	6	0.115797
9	8.65	762	89.8	0.0687547	5	0.605697	6	4.7	8.65	762.1	5	0.0689142
10	9.3	819	96.5	0.133854	6	1.31653	13	10.2	9.3	818.8	5.6	0.132141

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.259	51	6	0.152784	9	2.29967	8.9	6	4.259	52.5	8.9	0.145653
2	4.491	85	10	0.181553	8	2.55616	9.9	6.7	4.491	86.4	8.1	0.178009
3	4.675	112	13.2	0.156643	5	1.38169	5.4	3.6	4.675	112.2	5.1	0.154104
4	4.9	145	17	0.0981868	11	1.7591	6.8	4.6	4.9	144	10.7	0.0927935
5	5.03	164	19.3	0.0121193	1	0.0028802	0	0	5.03	165.3	0.5	0.0027101
6	5.2	189	22.2	0.0344684	3	0.203616	0.8	0.5	5.2	188.4	3.3	0.0350931
7	5.85	296	34.8	0.0429128	4	0.301811	1.2	0.8	5.85	295.4	4	0.0420811
8	5.912	308	36.2	0.306561	3	1.60594	6.2	4.2	5.912	307.8	3.1	0.288335
9	5.964	318	37.4	0.37088	7	4.21889	16.4	11	5.964	319.4	6.8	0.350197
10	6.1	344	40.4	0.168493	3	0.928358	3.6	2.4	6.1	344.1	3.2	0.164039
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.177	359	42.2	0.261526	2	1.13192	4.4	3	6.177	358.5	2.4	0.26483
12	6.302	383	45	0.0690043	6	0.758723	2.9	2	6.302	383.5	6.2	0.0685452
13	6.432	408	47.9	0.0901901	10	1.69919	6.6	4.4	6.432	413.1	10.4	0.0921411
14	6.474	416	48.9	0.118568	20	2.65196	10.3	6.9	6.474	436.4	19.6	0.0763177
15	6.85	459	53.9	0.0347521	4	0.135421	0.5	0.4	6.85	458.5	4.3	0.0178078
16	7.41	533	62.6	0.138262	9	2.16705	8.4	5.7	7.41	531.7	9.3	0.132087
17	7.682	575	67.6	0.12192	4	0.787305	3.1	2.1	7.682	575	3.7	0.119334
18	8.45	716	84.1	0.0245053	6	0.26838	1	0.7	8.45	716.8	6.2	0.0244902
19	8.65	765	89.9	0.020436	4	0.163295	0.6	0.4	8.65	764.3	4.4	0.0209876
20	9.3	820	96.4	0.0661809	7	0.762733	3	2	9.3	820.2	7.1	0.0603005

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.317	56	6.6	0.0947799	9	1.60282	6.4	5.1	4.317	57.8	9.5	0.0952783
2	4.507	84	9.9	0.284768	8	3.88106	15.6	12.2	4.507	86.1	8	0.27531
3	4.711	114	13.4	0.236271	4	1.3664	5.5	4.3	4.711	113.6	4.3	0.178671
4	4.806	128	15.1	0.12995	10	2.08929	8.4	6.6	4.806	122.5	10.1	0.116189
5	4.867	137	16.1	0.134301	14	3.11828	12.5	9.8	4.867	142.2	13.5	0.130026
6	5.078	168	19.8	0.008922	1	0	0	0	5.078	165	1	0
7	5.944	310	36.5	0.357473	3	2.1437	8.6	6.8	5.944	310	3.4	0.358124
8	5.99	319	37.5	0.280635	4	1.69761	6.8	5.4	5.99	318.6	3.8	0.254688
9	6.025	326	38.4	0.158793	5	1.21694	4.9	3.8	6.025	326	4.7	0.147015
10	6.127	346	40.7	0.18292	4	1.14534	4.6	3.6	6.127	346.4	3.6	0.178227
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.208	362	42.6	0.252703	3	1.36213	5.5	4.3	6.208	361.4	3.2	0.241875
12	6.335	387	45.5	0.0876868	6	0.937199	3.8	3	6.335	387.5	6.1	0.0870402
13	6.457	411	48.4	0.0679375	7	0.436375	1.7	1.4	6.457	410.7	7	0.0354031
14	6.482	416	48.9	0.0801032	7	0.739951	3	2.3	6.482	417.4	7.3	0.057101
15	7.433	536	63.1	0.1156	9	2.2745	9.1	7.2	7.433	531.4	8.9	0.144596
16	7.707	578	68	0.140711	4	0.943435	3.8	3	7.707	578.2	3.9	0.137977

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	90	10.6	0.0822443	5	0.74836	7.5	6.2	4.55	90.4	5.2	0.0813141
2	5.2	186	21.9	0.0933555	5	0.830861	8.4	6.9	5.2	186.4	5.2	0.08974
3	5.85	290	34.1	0.165162	5	1.42271	14.3	11.8	5.85	289.8	4.8	0.167925
4	6.55	431	50.6	0.166241	5	1.55118	15.6	12.9	6.55	431	5.4	0.163153
5	6.85	461	54.2	0.032139	5	0.280121	2.8	2.3	6.85	461.1	5.2	0.0305238
6	7.35	526	61.8	0.0932928	5	0.783603	7.9	6.5	7.35	526.3	4.6	0.095521
7	8.15	647	76	0.0895773	9	1.32739	13.4	11	8.15	647.1	8.5	0.0877718
8	8.45	708	83.2	0.124243	6	1.4072	14.2	11.7	8.45	707.7	6.4	0.124133
9	8.65	759	89.2	0.0822587	5	0.66889	6.7	5.5	8.65	758.8	4.6	0.0815926
10	9.3	818	96.1	0.106356	5	0.895172	9	7.4	9.3	817.5	4.5	0.111497

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.700 5.200 11.800 5.850 14.900 6.550 15.200 6.850 3.100 7.350 7.000 8.150 13.000 8.450 14.800 8.650 6.800 9.300 7.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.242 11.700 4.504 8.600 4.669 8.000 4.821 15.500 4.917 1.500 5.035 0 5.200 1.900 5.850 2.200 5.918 6.400 5.976 9.100 6.124 4.600 6.213 4.800 6.355 1.900 6.487 0.700 6.513 4.900 6.550 0.700 6.850 0.400 7.386 7.400 7.670 2.300 8.450 2.100 8.650 1.400 9.300 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 22 Return to Lane Navigator
4.220 11.400 4.485 11.300 4.687 14.300 4.862 13.200 5.050 0 5.937 9.300 5.985 3.900 6.022 6.600 6.127 4.300 6.207 5.000 6.333 4.000 6.455 0.300 6.487 4.300 7.391 8.900 7.679 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 6.000 5.200 10.400 5.850 13.700 6.550 14.500 6.850 3.400 7.350 8.100 8.150 12.600 8.450 12.200 8.650 6.000 9.300 13.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.259 8.900 4.491 9.900 4.675 5.400 4.900 6.800 5.030 0 5.200 0.800 5.850 1.200 5.912 6.200 5.964 16.400 6.100 3.600 6.177 4.400 6.302 2.900 6.432 6.600 6.474 10.300 6.850 0.500 7.410 8.400 7.682 3.100 8.450 1.000 8.650 0.600 9.300 3.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
4.317 6.400 4.507 15.600 4.711 5.500 4.806 8.400 4.867 12.500 5.078 0 5.944 8.600 5.990 6.800 6.025 4.900 6.127 4.600 6.208 5.500 6.335 3.800 6.457 1.700 6.482 3.000 7.433 9.100 7.707 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 7.500 5.200 8.400 5.850 14.300 6.550 15.600 6.850 2.800 7.350 7.900 8.150 13.400 8.450 14.200 8.650 6.700 9.300 9.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.