RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19970220gel01
Unique Web Code: esh05Srr
Tissue Aliquot Code: pleuvetu I94-004 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	96	11.4	0.0525542	4	0.347607	3.8	2.8	4.55	96.5	4.1	0.048383
2	5.2	188	22.3	0.0930622	5	0.703343	7.6	5.6	5.2	188	4.6	0.0861169
3	5.85	300	35.6	0.0671337	5	0.544864	5.9	4.3	5.85	299.5	4.6	0.0670584
4	6.55	445	52.8	0.127442	7	1.33767	14.5	10.7	6.55	444.5	6.5	0.116045
5	6.85	477	56.6	0.0350879	6	0.348986	3.8	2.8	6.85	477.8	6.4	0.030963
6	7.35	543	64.4	0.0737744	4	0.569591	6.2	4.5	7.35	543.3	4.4	0.0729722
7	8.15	662	78.5	0.0988538	8	1.40856	15.3	11.2	8.15	662.6	8.5	0.093507
8	8.45	728	86.4	0.164581	6	1.7637	19.1	14.1	8.45	726.9	6.2	0.160465
9	8.65	779	92.4	0.130351	4	0.912906	9.9	7.3	8.65	778.7	4.2	0.121194
10	9.3	818	97	0.152693	5	1.27776	13.9	10.2	9.3	817.9	4.6	0.156764

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.967	15	1.8	0.0223353	3	0.127234	0.4	0.4	3.967	15.1	3.3	0.0219428
2	4.351	69	8.2	0.0544364	8	0.715587	2.3	2	4.351	70.3	7.7	0.0527442
3	4.443	82	9.7	0.203325	4	1.3951	4.5	4	4.443	81.7	4.2	0.185731
4	4.543	96	11.4	0.315814	10	4.99353	16.2	14.2	4.543	99.1	10.4	0.269842
5	4.735	123	14.6	0.112115	16	3.29152	10.7	9.3	4.735	118	15.7	0.118199
6	5.249	197	23.4	0.106246	4	0.80142	2.6	2.3	5.249	197.1	4.2	0.108472
7	5.313	208	24.7	0.0111673	3	0.0570046	0.2	0.2	5.313	208.1	2.7	0.0117766
8	5.406	224	26.6	0.211498	4	1.66275	5.4	4.7	5.406	224.9	4.5	0.210595
9	5.487	238	28.3	0.252095	3	1.41751	4.6	4	5.487	238.3	3.2	0.250468
10	5.569	252	29.9	0.0232199	4	0.130706	0.4	0.4	5.569	250.8	4	0.0182352
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.644	265	31.5	0.426044	4	2.78625	9	7.9	5.644	265.7	3.7	0.420306
12	5.737	281	33.4	0.172999	9	2.42695	7.9	6.9	5.737	280	8.9	0.154061
13	5.83	297	35.3	0.0436949	3	0.220238	0.7	0.6	5.83	297	3.1	0.0402573
14	5.901	311	36.9	0.233395	4	1.49772	4.9	4.2	5.901	310.5	3.6	0.232884
15	5.987	329	39.1	0.0269513	3	0.147384	0.5	0.4	5.987	329.1	3.1	0.0270967
16	6.136	360	42.8	0.0793592	12	1.40325	4.6	4	6.136	361	12.2	0.0647819
17	6.281	390	46.3	0.141244	12	2.51301	8.1	7.1	6.281	388.9	12.1	0.117104
18	6.382	411	48.8	0.170494	2	0.482607	1.6	1.4	6.382	411.3	1.8	0.151622
19	6.516	439	52.1	0.0506455	7	0.635727	2.1	1.8	6.516	438	7.4	0.0486643
20	7.256	530	62.9	0.162546	16	4.13225	13.4	11.7	7.256	529.2	15.9	0.147093

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	98	11.7	0.0323938	4	0.241256	3.1	2.3	4.55	98.1	4.2	0.0322253
2	5.2	189	22.5	0.0516965	7	0.650101	8.5	6.3	5.2	189.2	7.3	0.0503102
3	5.85	301	35.8	0.0617695	5	0.533299	6.9	5.2	5.85	301.1	4.9	0.061164
4	6.55	447	53.2	0.103562	7	1.17642	15.3	11.5	6.55	445.7	7.2	0.0928045
5	6.85	479	57	0.0279567	5	0.237973	3.1	2.3	6.85	480.3	5.5	0.0245774
6	7.35	541	64.3	0.0632376	4	0.430988	5.6	4.2	7.35	541	4	0.0614262
7	8.15	662	78.7	0.0832827	7	1.03202	13.4	10.1	8.15	661.9	7.2	0.0806058
8	8.45	729	86.7	0.117177	6	1.31029	17.1	12.8	8.45	728.1	6.4	0.115624
9	8.65	779	92.6	0.0990202	4	0.685372	8.9	6.7	8.65	779.2	4.1	0.0948178
10	9.3	817	97.1	0.157401	5	1.37842	18	13.4	9.3	817.3	4.8	0.162281

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.98	15	1.8	0.0238396	4	0.153781	0.6	0.6	3.98	15.1	3.8	0.0227809
2	4.387	74	8.8	0.0350116	5	0.292843	1.2	1.1	4.387	74	4.7	0.0354221
3	4.518	93	11.1	0.0533464	5	0.51178	2.1	1.9	4.518	91.4	5.4	0.0537298
4	4.614	107	12.7	0.227863	7	2.51237	10.4	9.3	4.614	107.6	7.5	0.18977
5	4.766	129	15.4	0.0666959	14	2.07108	8.6	7.7	4.766	121	14.3	0.0814309
6	5.2	192	22.9	0.0456516	5	0.331223	1.4	1.2	5.2	191.3	4.5	0.0410939
7	5.253	201	23.9	0.0629968	5	0.560301	2.3	2.1	5.253	200.3	5	0.0627442
8	5.413	228	27.1	0.0927397	6	0.945159	3.9	3.5	5.413	228.2	5.5	0.0965789
9	5.495	242	28.8	0.123172	4	0.953434	4	3.5	5.495	241.7	4.2	0.128762
10	5.584	257	30.6	0.00818789	2	0.0250907	0.1	0.1	5.584	256.3	2.1	0.0068623
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.661	270	32.1	0.341367	4	2.47982	10.3	9.2	5.661	270.4	4.2	0.333113
12	5.75	285	33.9	0.160174	8	2.07473	8.6	7.7	5.75	285.2	7.8	0.150357
13	5.85	302	36	0.0678625	7	0.775402	3.2	2.9	5.85	303.4	6.6	0.0662006
14	5.919	316	37.6	0.283373	3	1.61717	6.7	6	5.919	315.3	3.3	0.27534
15	5.998	332	39.5	0.0324036	4	0.205137	0.8	0.8	5.998	332.1	3.8	0.0303891
16	6.136	360	42.9	0.0231963	8	0.23065	1	0.9	6.136	359	7.6	0.0171748
17	6.284	390	46.4	0.136869	12	2.3437	9.7	8.7	6.284	388.9	11.6	0.113558
18	6.402	414	49.3	0.118877	4	0.645021	2.7	2.4	6.402	414.8	3.6	0.0999653
19	6.55	444	52.9	0.0705187	8	0.986993	4.1	3.7	6.55	442.2	7.9	0.070844
20	6.85	480	57.1	0.0304249	7	0.361613	1.5	1.3	6.85	480.9	7	0.0293172
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.267	530	63.1	0.0764024	11	1.4819	6.1	5.5	7.267	530.7	11.2	0.0748987
22	7.35	540	64.3	0.102793	4	0.479254	2	1.8	7.35	540.5	4	0.0673321
23	8.15	666	79.3	0.03072	9	0.482764	2	1.8	8.15	665.4	9.4	0.0289619
24	8.45	732	87.1	0.060285	7	0.708696	2.9	2.6	8.45	730.8	6.9	0.0575587
25	8.65	781	93	0.0512211	3	0.291332	1.2	1.1	8.65	780.5	3.3	0.0503585
26	9.3	818	97.4	0.0745894	5	0.616091	2.6	2.3	9.3	818.2	4.6	0.0754763

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.984	18	2.1	0.0274662	4	0.192486	0.7	0.6	3.984	18.4	4.1	0.0265552
2	4.398	76	9.1	0.00809522	2	0.0227499	0.1	0.1	4.398	76.1	2.2	0.0057534
3	4.491	89	10.6	0.16164	4	0.893142	3.3	2.7	4.491	87.8	3.7	0.137886
4	4.598	104	12.4	0.254129	13	5.41459	20.3	16.5	4.598	106.4	12.6	0.24271
5	4.776	129	15.4	0.0786263	7	0.785719	2.9	2.4	4.776	128.7	7.2	0.0617786
6	5.277	202	24.1	0.142571	3	0.828532	3.1	2.5	5.277	202.3	3.3	0.143597
7	5.346	214	25.5	0.0157566	2	0.0499121	0.2	0.2	5.346	214.3	1.8	0.0156359
8	5.438	230	27.4	0.229254	4	1.42762	5.3	4.4	5.438	230.9	3.9	0.207337
9	5.529	246	29.3	0.264708	2	1.15318	4.3	3.5	5.529	245.6	2.4	0.26712
10	5.615	261	31.1	0.0184143	1	0	0	0	5.615	257	1	0
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.684	273	32.5	0.451078	4	2.78343	10.4	8.5	5.684	273.6	3.5	0.443686
12	5.77	288	34.3	0.180434	8	2.26572	8.5	6.9	5.77	287.1	8.3	0.154834
13	5.855	303	36.1	0.0477448	3	0.21334	0.8	0.7	5.855	302.8	2.8	0.043513
14	5.92	316	37.7	0.294426	3	1.63212	6.1	5	5.92	315.3	3.2	0.287657
15	5.994	331	39.5	0.0530478	3	0.259315	1	0.8	5.994	331.1	2.8	0.051758
16	6.133	359	42.8	0.0576954	11	0.81799	3.1	2.5	6.133	360	10.6	0.0437297
17	6.282	389	46.4	0.145471	13	2.56463	9.6	7.8	6.282	388.4	12.6	0.114997
18	6.401	413	49.2	0.192455	2	0.635086	2.4	1.9	6.401	412.9	2	0.175394
19	6.55	443	52.8	0.041263	9	0.665721	2.5	2	6.55	438.8	8.7	0.04325
20	7.241	527	62.8	0.143848	18	4.09582	15.3	12.5	7.241	525.3	18	0.128381

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	97	11.6	0.0283493	4	0.203296	2.5	1.9	4.55	96.8	4.1	0.0276635
2	5.2	185	22.1	0.0504541	6	0.54911	6.7	5.1	5.2	185.2	6.5	0.0479695
3	5.85	302	36.1	0.0775533	4	0.583133	7.2	5.4	5.85	301.8	4.2	0.0787322
4	6.55	442	52.9	0.136884	6	1.38387	17	12.9	6.55	441.1	6.1	0.128989
5	6.85	473	56.6	0.0435748	5	0.361728	4.4	3.4	6.85	473.8	4.9	0.0416528
6	7.35	542	64.8	0.0752021	4	0.52279	6.4	4.9	7.35	541.5	3.9	0.0753675
7	8.15	662	79.2	0.0815947	7	1.00035	12.3	9.3	8.15	661.9	7.2	0.0784056
8	8.45	727	87	0.125665	6	1.40276	17.2	13.1	8.45	726.9	6.2	0.127973
9	8.65	780	93.3	0.111989	4	0.821311	10.1	7.7	8.65	779.9	4.5	0.10403
10	9.3	820	98.1	0.159218	5	1.32229	16.2	12.3	9.3	820.3	4.6	0.163829

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 96

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 3.800 5.200 7.600 5.850 5.900 6.550 14.500 6.850 3.800 7.350 6.200 8.150 15.300 8.450 19.100 8.650 9.900 9.300 13.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.967 0.400 4.351 2.300 4.443 4.500 4.543 16.200 4.735 10.700 5.249 2.600 5.313 0.200 5.406 5.400 5.487 4.600 5.569 0.400 5.644 9.000 5.737 7.900 5.830 0.700 5.901 4.900 5.987 0.500 6.136 4.600 6.281 8.100 6.382 1.600 6.516 2.100 7.256 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 3.100 5.200 8.500 5.850 6.900 6.550 15.300 6.850 3.100 7.350 5.600 8.150 13.400 8.450 17.100 8.650 8.900 9.300 18.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.980 0.600 4.387 1.200 4.518 2.100 4.614 10.400 4.766 8.600 5.200 1.400 5.253 2.300 5.413 3.900 5.495 4.000 5.584 0.100 5.661 10.300 5.750 8.600 5.850 3.200 5.919 6.700 5.998 0.800 6.136 1.000 6.284 9.700 6.402 2.700 6.550 4.100 6.850 1.500 7.267 6.100 7.350 2.000 8.150 2.000 8.450 2.900 8.650 1.200 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 26 Return to Lane Navigator
3.984 0.700 4.398 0.100 4.491 3.300 4.598 20.300 4.776 2.900 5.277 3.100 5.346 0.200 5.438 5.300 5.529 4.300 5.615 0 5.684 10.400 5.770 8.500 5.855 0.800 5.920 6.100 5.994 1.000 6.133 3.100 6.282 9.600 6.401 2.400 6.550 2.500 7.241 15.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 2.500 5.200 6.700 5.850 7.200 6.550 17.000 6.850 4.400 7.350 6.400 8.150 12.300 8.450 17.200 8.650 10.100 9.300 16.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.