RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lepidocybium(*) flavobrunneum(*)
FDA Seafood List Common Name: Escolar(*)
FDA Seafood List Market Name: Escolar(*)
Unique IEF Gel Code: 19950831gel11
Unique Web Code: ech03Srr
Tissue Aliquot Code: lepiflav I94-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	49	6.1	0.0199508	6	0.203548	10.7	7.4	4.55	50.6	6.3	0.0182722
2	5.2	143	17.9	0.0128826	7	0.14113	7.4	5.2	5.2	142.9	6.6	0.0120092
3	5.85	253	31.7	0.0197564	6	0.196281	10.3	7.2	5.85	253.9	5.8	0.0190694
4	6.55	378	47.4	0.0213942	13	0.440153	23	16.1	6.55	374.4	13.2	0.018786
5	7.35	480	60.2	0.0047011	10	0.0682991	3.6	2.5	7.35	481.9	9.7	0.0039552
6	8.15	606	75.9	0.0140258	9	0.190961	10	7	8.15	603.9	8.8	0.012301
7	8.45	668	83.7	0.0162922	9	0.244749	12.8	8.9	8.45	668.3	9.1	0.0152501
8	8.65	718	90	0.0104736	10	0.166937	8.7	6.1	8.65	718.1	9.7	0.0097299
9	9.3	767	96.1	0.0125633	13	0.258684	13.5	9.5	9.3	767.4	13	0.0111983

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.014	285	35.7	0.0137122	7	0.14395	0.8	0.6	6.014	285.2	7.3	0.0110687
2	6.069	295	37	0.0164307	3	0.072196	0.4	0.3	6.069	295.3	2.7	0.015158
3	6.169	313	39.2	0.153548	3	0.952289	5.4	3.8	6.169	312.9	3.5	0.153895
4	6.247	327	41	0.0564662	4	0.388464	2.2	1.6	6.247	327.8	3.8	0.0575606
5	6.336	343	43	0.0941536	6	0.993609	5.6	4	6.336	342.4	6.1	0.0922884
6	6.392	353	44.2	0.0608161	4	0.445658	2.5	1.8	6.392	354	4.5	0.0562612
7	6.604	388	48.6	0.255835	3	1.44493	8.1	5.8	6.604	388	3.2	0.253782
8	6.67	396	49.6	0.357328	3	2.10271	11.9	8.5	6.67	395.8	3.3	0.35891
9	6.761	407	51	0.450495	4	3.1265	17.6	12.6	6.761	407	3.9	0.44961
10	6.902	424	53.1	0.136539	4	0.837247	4.7	3.4	6.902	423.9	3.7	0.127096
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.985	434	54.4	0.131027	6	1.18198	6.7	4.8	6.985	433.2	6.3	0.105322
12	7.06	443	55.5	0.180274	7	2.24796	12.7	9.1	7.06	444	7.4	0.170545
13	7.201	460	57.6	0.263457	4	2.00664	11.3	8.1	7.201	460.1	4.3	0.264054
14	7.317	474	59.4	0.0147935	3	0.0638105	0.4	0.3	7.317	474.5	2.5	0.0141779
15	7.903	564	70.7	0.285557	3	1.72917	9.7	7	7.903	564.4	3.4	0.286273

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	50	6.3	0.0167059	7	0.210814	14.6	8.8	4.55	50.1	6.9	0.0171841
2	5.2	141	17.8	0.0110115	11	0.185974	12.9	7.8	5.2	139.7	10.7	0.0098351
3	5.85	254	32	0.0172046	7	0.20573	14.3	8.6	5.85	253.4	7.1	0.0164343
4	6.55	381	48	0.0173701	5	0.146224	10.1	6.1	6.55	380.2	4.6	0.0177435
5	7.35	472	59.4	0.0055307	7	0.0538985	3.7	2.3	7.35	472	6.5	0.0046528
6	8.15	595	74.9	0.0135308	7	0.151462	10.5	6.3	8.15	595.4	6.6	0.0129346
7	8.45	656	82.6	0.0152542	9	0.207875	14.4	8.7	8.45	654.6	8.9	0.0131302
8	8.65	704	88.7	0.0069089	5	0.0559578	3.9	2.3	8.65	703.8	5.1	0.0061741
9	9.3	763	96.1	0.0147185	10	0.222798	15.5	9.3	9.3	762.8	10	0.0125112

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	50	6.3	0.0080063	6	0.0901292	0.7	0.7	4.55	51.4	6	0.0084801
2	5.2	144	18.3	0.0063166	9	0.0846592	0.7	0.6	5.2	145.9	8.7	0.0054968
3	5.85	260	33	0.0136649	8	0.173724	1.4	1.3	5.85	257.4	7.7	0.0127975
4	6.186	316	40.1	0.0878047	3	0.491112	3.9	3.6	6.186	316.1	3.2	0.0862955
5	6.276	331	42	0.0423766	4	0.273427	2.2	2	6.276	330.7	3.7	0.0413929
6	6.348	343	43.5	0.0583727	6	0.5897	4.7	4.3	6.348	342.9	5.7	0.0580795
7	6.408	353	44.7	0.0364478	4	0.245563	2	1.8	6.408	354.3	4.1	0.0341186
8	6.522	372	47.1	0.0201769	4	0.148252	1.2	1.1	6.522	372.9	4.1	0.0203164
9	6.622	385	48.8	0.137093	3	0.707276	5.7	5.1	6.622	385.4	2.9	0.135917
10	6.7	394	49.9	0.253978	3	1.4103	11.3	10.2	6.7	394.2	3.2	0.249949
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.795	405	51.3	0.36751	4	2.45238	19.7	17.8	6.795	404.6	3.7	0.372451
12	6.933	421	53.4	0.068345	3	0.406773	3.3	2.9	6.933	420.5	3.4	0.0677968
13	7.002	429	54.4	0.0748173	5	0.476769	3.8	3.5	7.002	428.6	4.8	0.0565783
14	7.079	438	55.5	0.0866728	7	1.06483	8.6	7.7	7.079	437.9	7	0.0858462
15	7.218	454	57.5	0.166611	4	1.20513	9.7	8.7	7.218	453.8	4	0.168842
16	7.33	467	59.2	0.0125262	4	0.0911108	0.7	0.7	7.33	469.3	4.2	0.0122212
17	7.72	525	66.5	0.0611201	11	1.07677	8.7	7.8	7.72	522.5	10.6	0.0570848
18	7.926	556	70.5	0.144462	4	1.01683	8.2	7.4	7.926	556.4	4	0.142673
19	8.45	661	83.8	0.0101426	13	0.246625	2	1.8	8.45	665.3	12.7	0.010928
20	8.65	702	89	0.0139287	9	0.192079	1.5	1.4	8.65	702.4	8.7	0.0124634

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.981	280	35.6	0.0078455	5	0.0576913	0.4	0.3	5.981	281.8	5.1	0.0064008
2	6.047	291	37	0.013526	3	0.0685597	0.5	0.4	6.047	291.2	3.2	0.0120542
3	6.148	308	39.1	0.11709	4	0.766472	5.4	4.1	6.148	308.6	3.7	0.118323
4	6.256	326	41.4	0.0563218	4	0.421171	3	2.2	6.256	325.7	4.4	0.054027
5	6.345	341	43.3	0.0644762	7	0.758084	5.4	4	6.345	339.9	6.5	0.0656555
6	6.411	352	44.7	0.0418976	4	0.2643	1.9	1.4	6.411	352.6	3.8	0.0394087
7	6.518	370	47	0.0205098	3	0.12604	0.9	0.7	6.518	370.2	3.4	0.0209779
8	6.607	382	48.5	0.169516	3	0.935194	6.6	5	6.607	382.2	3.1	0.168106
9	6.674	390	49.6	0.273349	4	1.74585	12.4	9.3	6.674	390.3	3.6	0.272785
10	6.759	400	50.8	0.396466	4	2.7347	19.4	14.5	6.759	400.2	3.9	0.399258
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.886	415	52.7	0.0918392	3	0.51655	3.7	2.7	6.886	415.2	3.4	0.0864777
12	6.963	424	53.9	0.104488	5	0.683324	4.8	3.6	6.963	422.8	4.7	0.0812181
13	7.031	432	54.9	0.142056	7	1.6533	11.7	8.8	7.031	432.5	6.7	0.139877
14	7.166	448	56.9	0.230502	4	1.69065	12	9	7.166	448	4.1	0.232769
15	7.293	463	58.8	0.0091711	3	0.0510807	0.4	0.3	7.293	463	3.3	0.008709
16	7.903	551	70	0.252022	4	1.65958	11.7	8.8	7.903	550.8	3.7	0.252306

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	47	6	0.0187955	6	0.189792	11.6	7.4	4.55	47.3	6	0.0179193
2	5.2	147	18.7	0.0154302	7	0.1808	11.1	7.1	5.2	147.1	7.5	0.0136133
3	5.85	258	32.8	0.0193247	6	0.206101	12.6	8.1	5.85	258.5	6.4	0.0181461
4	6.55	376	47.8	0.0229047	8	0.313153	19.2	12.3	6.55	373.9	8.5	0.0208805
5	7.35	472	60	0.008285	8	0.103037	6.3	4	7.35	470.3	8.4	0.0068984
6	8.15	587	74.6	0.0210029	6	0.214319	13.1	8.4	8.15	587.5	5.7	0.0213205
7	8.45	652	82.8	0.0129025	10	0.214161	13.1	8.4	8.45	651.3	9.8	0.0123681
8	8.65	694	88.2	0.0107123	8	0.152153	9.3	6	8.65	693.8	8.5	0.010118
9	9.3	752	95.6	0.006035	6	0.0594955	3.6	2.3	9.3	753	6.2	0.0054151

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 78

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.700 5.200 7.400 5.850 10.300 6.550 23.000 7.350 3.600 8.150 10.000 8.450 12.800 8.650 8.700 9.300 13.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
6.014 0.800 6.069 0.400 6.169 5.400 6.247 2.200 6.336 5.600 6.392 2.500 6.604 8.100 6.670 11.900 6.761 17.600 6.902 4.700 6.985 6.700 7.060 12.700 7.201 11.300 7.317 0.400 7.903 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 14.600 5.200 12.900 5.850 14.300 6.550 10.100 7.350 3.700 8.150 10.500 8.450 14.400 8.650 3.900 9.300 15.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 0.700 5.200 0.700 5.850 1.400 6.186 3.900 6.276 2.200 6.348 4.700 6.408 2.000 6.522 1.200 6.622 5.700 6.700 11.300 6.795 19.700 6.933 3.300 7.002 3.800 7.079 8.600 7.218 9.700 7.330 0.700 7.720 8.700 7.926 8.200 8.450 2.000 8.650 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
5.981 0.400 6.047 0.500 6.148 5.400 6.256 3.000 6.345 5.400 6.411 1.900 6.518 0.900 6.607 6.600 6.674 12.400 6.759 19.400 6.886 3.700 6.963 4.800 7.031 11.700 7.166 12.000 7.293 0.400 7.903 11.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 11.600 5.200 11.100 5.850 12.600 6.550 19.200 7.350 6.300 8.150 13.100 8.450 13.100 8.650 9.300 9.300 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Escolar(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.