RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lepidocybium(*) flavobrunneum(*)
FDA Seafood List Common Name: Escolar(*)
FDA Seafood List Market Name: Escolar(*)
Unique IEF Gel Code: 19950830gel16
Unique Web Code: ecg04Srr
Tissue Aliquot Code: lepiflav I94-003 #03 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.6	0.0802829	11	1.54813	13.4	8.9	3.5	44.2	11.3	0.0772341
2	4.55	182	16.2	0.123427	4	0.832086	7.2	4.8	4.55	182	4	0.116358
3	5.2	262	23.4	0.1014	4	0.771656	6.7	4.4	5.2	262.5	4.5	0.0973711
4	5.85	417	37.2	0.228749	3	1.28868	11.2	7.4	5.85	417	3.2	0.226929
5	6.55	596	53.1	0.122953	5	0.887632	7.7	5.1	6.55	595.4	4.5	0.110935
6	6.85	635	56.6	0.0541206	8	0.605228	5.2	3.5	6.85	635.6	7.6	0.0447586
7	7.35	728	64.9	0.117996	5	0.933571	8.1	5.4	7.35	727.6	4.6	0.113639
8	8.15	886	79	0.0346854	10	0.535688	4.6	3.1	8.15	885.7	9.7	0.0313047
9	8.45	948	84.5	0.059895	7	0.704084	6.1	4	8.45	947.2	7.3	0.0545475
10	8.65	986	87.9	0.0602594	7	0.650877	5.6	3.7	8.65	985.3	6.7	0.0552051
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1108	98.8	0.206732	8	2.78526	24.1	16	9.3	1105.9	8.1	0.195048

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.104829	11	1.79027	9.8	7.2	3.5	39.4	10.8	0.0932272
2	4.55	192	17.1	0.0728407	8	0.960452	5.3	3.9	4.55	194.7	8	0.0677862
3	5.2	279	24.9	0.0522039	11	0.916569	5	3.7	5.2	279.4	11.5	0.044994
4	5.85	426	38	0.143091	4	1.13364	6.2	4.5	5.85	425.9	4.3	0.148247
5	6.062	477	42.6	0.0329778	8	0.366855	2	1.5	6.062	476.6	7.6	0.0273687
6	6.158	500	44.6	0.192476	2	0.860941	4.7	3.5	6.158	500.5	2.5	0.195184
7	6.263	525	46.9	0.056604	5	0.493101	2.7	2	6.263	525.1	4.8	0.0582206
8	6.308	536	47.9	0.0325448	5	0.275696	1.5	1.1	6.308	538.8	4.9	0.0315815
9	6.4	558	49.8	0.029049	5	0.19648	1.1	0.8	6.4	556.8	5.1	0.0219153
10	6.45	570	50.9	0.00527108	11	0.268396	1.5	1.1	6.45	569.2	10.9	0.013864
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.55	594	53	0.0779786	3	0.489572	2.7	2	6.55	594.5	3.4	0.0817436
12	6.602	601	53.7	0.0959448	2	0.299181	1.6	1.2	6.602	601.2	1.9	0.0884737
13	6.655	608	54.3	0.257983	2	0.78004	4.3	3.1	6.655	607.7	1.7	0.25601
14	6.73	618	55.2	0.041627	2	0.156292	0.9	0.6	6.73	617.3	1.8	0.0489901
15	6.85	634	56.6	0.275034	3	1.28087	7	5.1	6.85	634.5	2.7	0.271157
16	6.961	654	58.4	0.060616	3	0.271688	1.5	1.1	6.961	654.3	2.6	0.0582272
17	7.25	706	63	0.18237	3	0.920316	5.1	3.7	7.25	706	2.8	0.182873
18	7.35	724	64.6	0.093799	4	0.713614	3.9	2.9	7.35	723.9	4.3	0.0934416
19	7.696	792	70.7	0.0486212	13	1.69228	9.3	6.8	7.696	804.5	13.1	0.0729554
20	7.916	835	74.6	0.113934	5	0.932019	5.1	3.7	7.916	834.9	4.6	0.114396
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	881	78.7	0.0280374	8	0.368861	2	1.5	8.15	881.4	7.9	0.0264283
22	8.45	945	84.4	0.0156333	5	0.131639	0.7	0.5	8.45	944.5	4.6	0.0161629
23	8.65	983	87.8	0.0142329	4	0.173475	1	0.7	8.65	986	4.1	0.024053
24	9.3	1104	98.6	0.203215	8	2.73989	15	11	9.3	1101.9	8.4	0.184231

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.038	473	42.1	0.0373289	5	0.243699	2.7	1.6	6.038	472.7	4.7	0.029193
2	6.132	496	44.2	0.212772	2	0.845844	9.3	5.7	6.132	496	2.3	0.210536
3	6.234	521	46.4	0.0659187	4	0.459831	5.1	3.1	6.234	520.7	4.1	0.0630821
4	6.295	536	47.7	0.0295737	4	0.219743	2.4	1.5	6.295	534.3	4.5	0.0277722
5	6.352	550	49	0.044686	3	0.263166	2.9	1.8	6.352	551.6	3.5	0.0427943
6	6.397	561	50	0.0266668	3	0.150232	1.7	1	6.397	561.3	3.2	0.0262678
7	6.434	570	50.8	0.0383551	4	0.197716	2.2	1.3	6.434	570.3	3.7	0.0305353
8	6.511	589	52.4	0.0277293	3	0.16049	1.8	1.1	6.511	589.2	3.2	0.028676
9	6.548	598	53.3	0.0424612	3	0.302784	3.3	2	6.548	596.7	3	0.0572702
10	6.584	603	53.7	0.244319	2	0.763252	8.4	5.1	6.584	603.3	1.8	0.240466
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.66	613	54.6	0.0409635	3	0.216531	2.4	1.5	6.66	613.7	3.3	0.0366271
12	6.797	631	56.2	0.289522	2	1.1978	13.2	8.1	6.797	631	2.4	0.287481
13	6.91	649	57.8	0.0546076	3	0.28436	3.1	1.9	6.91	649	3	0.0527324
14	7.194	701	62.4	0.228099	2	0.947384	10.4	6.4	7.194	701.2	2.3	0.230012
15	7.659	790	70.3	0.067696	18	1.81264	19.9	12.2	7.659	795.2	17.7	0.0576384
16	7.869	831	74	0.183302	3	1.02317	11.3	6.9	7.869	830.8	3.3	0.177075

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	35	3.1	0.0823077	8	1.1062	9.8	6.3	3.5	38.2	8	0.0779753
2	4.55	195	17.3	0.14676	4	0.972724	8.7	5.5	4.55	194.7	4.1	0.133963
3	5.2	282	25.1	0.133584	7	1.27752	11.4	7.3	5.2	280.3	6.6	0.109523
4	5.85	425	37.8	0.229304	3	1.34326	11.9	7.7	5.85	425.2	3.3	0.230726
5	6.55	600	53.4	0.13613	4	0.978557	8.7	5.6	6.55	599.6	4.4	0.12665
6	6.85	639	56.9	0.052157	6	0.492991	4.4	2.8	6.85	639.3	6.5	0.0430333
7	7.35	732	65.1	0.123589	5	1.03445	9.2	5.9	7.35	730.9	5	0.115907
8	8.15	888	79	0.037501	8	0.475056	4.2	2.7	8.15	887.4	7.8	0.0342578
9	8.45	948	84.3	0.0551591	7	0.651935	5.8	3.7	8.45	947.7	7	0.0522308
10	8.65	985	87.6	0.0541049	6	0.544069	4.8	3.1	8.65	984.5	5.9	0.0518147
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1104	98.2	0.180451	8	2.36397	21	13.5	9.3	1102.2	8	0.166242

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.044	474	42.1	0.0352637	5	0.28242	3.3	2	6.044	474.7	4.9	0.03226
2	6.137	497	44.1	0.21076	2	0.836943	9.9	6	6.137	497.2	2.3	0.205729
3	6.234	521	46.2	0.0697387	4	0.52944	6.3	3.8	6.234	521	4.4	0.0673799
4	6.291	535	47.5	0.0284245	5	0.254629	3	1.8	6.291	534.8	4.9	0.0295812
5	6.364	553	49.1	0.0386447	4	0.222219	2.6	1.6	6.364	552.4	3.7	0.034265
6	6.396	561	49.8	0.0285623	2	0.100709	1.2	0.7	6.396	560.5	2.4	0.024085
7	6.429	569	50.5	0.030066	6	0.270773	3.2	1.9	6.429	569.7	6.2	0.0246502
8	6.514	590	52.4	0.0275623	3	0.160556	1.9	1.2	6.514	589.3	3.4	0.0267442
9	6.534	595	52.8	0.0504307	2	0.233081	2.8	1.7	6.534	596.1	1.9	0.0688382
10	6.571	602	53.4	0.217148	2	0.698011	8.2	5	6.571	602.1	1.9	0.208807
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.643	612	54.3	0.0361376	6	0.337639	4	2.4	6.643	614.8	5.9	0.0321545
12	6.771	630	55.9	0.287616	2	1.08746	12.9	7.8	6.771	630.3	2.2	0.281411
13	6.889	648	57.5	0.060622	7	0.554276	6.6	4	6.889	647.7	6.7	0.0466323
14	7.18	700	62.1	0.224029	2	0.924597	10.9	6.6	7.18	700.2	2.3	0.225505
15	7.613	782	69.4	0.0378679	14	1.0555	12.5	7.6	7.613	788.2	14.2	0.0418774
16	7.854	829	73.6	0.148284	3	0.913227	10.8	6.5	7.854	829.2	3.5	0.148636

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	30	2.7	0.0873698	9	1.32485	10.7	7.5	3.5	32.3	9.3	0.0804672
2	4.55	190	16.9	0.122896	5	0.977453	7.9	5.5	4.55	190.1	4.7	0.117398
3	5.2	275	24.4	0.132234	4	0.938669	7.6	5.3	5.2	274.9	4.1	0.127626
4	5.85	421	37.4	0.267435	3	1.27015	10.3	7.2	5.85	420.6	2.7	0.267914
5	6.55	592	52.6	0.133962	4	0.857505	6.9	4.9	6.55	591.2	3.8	0.126359
6	6.85	637	56.6	0.0862032	9	0.823822	6.7	4.7	6.85	634.1	8.9	0.0523799
7	7.35	723	64.3	0.136613	4	1.03369	8.4	5.9	7.35	722.8	4.4	0.131469
8	8.15	880	78.2	0.0332754	7	0.406818	3.3	2.3	8.15	879.7	7.5	0.0307096
9	8.45	943	83.8	0.0589459	6	0.613867	5	3.5	8.45	942.6	6	0.0575305
10	8.65	981	87.2	0.0590042	6	0.647723	5.2	3.7	8.65	981.5	6.3	0.0582347
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1099	97.7	0.18947	11	3.47817	28.1	19.7	9.3	1094.6	11.3	0.174102

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 89

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 13.400 4.550 7.200 5.200 6.700 5.850 11.200 6.550 7.700 6.850 5.200 7.350 8.100 8.150 4.600 8.450 6.100 8.650 5.600 9.300 24.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 9.800 4.550 5.300 5.200 5.000 5.850 6.200 6.062 2.000 6.158 4.700 6.263 2.700 6.308 1.500 6.400 1.100 6.450 1.500 6.550 2.700 6.602 1.600 6.655 4.300 6.730 0.900 6.850 7.000 6.961 1.500 7.250 5.100 7.350 3.900 7.696 9.300 7.916 5.100 8.150 2.000 8.450 0.700 8.650 1.000 9.300 15.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 24 Return to Lane Navigator
6.038 2.700 6.132 9.300 6.234 5.100 6.295 2.400 6.352 2.900 6.397 1.700 6.434 2.200 6.511 1.800 6.548 3.300 6.584 8.400 6.660 2.400 6.797 13.200 6.910 3.100 7.194 10.400 7.659 19.900 7.869 11.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 16 Return to Lane Navigator
3.500 9.800 4.550 8.700 5.200 11.400 5.850 11.900 6.550 8.700 6.850 4.400 7.350 9.200 8.150 4.200 8.450 5.800 8.650 4.800 9.300 21.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
6.044 3.300 6.137 9.900 6.234 6.300 6.291 3.000 6.364 2.600 6.396 1.200 6.429 3.200 6.514 1.900 6.534 2.800 6.571 8.200 6.643 4.000 6.771 12.900 6.889 6.600 7.180 10.900 7.613 12.500 7.854 10.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 16 Return to Lane Navigator
3.500 10.700 4.550 7.900 5.200 7.600 5.850 10.300 6.550 6.900 6.850 6.700 7.350 8.400 8.150 3.300 8.450 5.000 8.650 5.200 9.300 28.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Escolar(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.