RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lepidocybium(*) flavobrunneum(*)
FDA Seafood List Common Name: Escolar(*)
FDA Seafood List Market Name: Escolar(*)
Unique IEF Gel Code: 19970423gel03
Unique Web Code: ecg01Srr
Tissue Aliquot Code: lepiflav L95-001 #15p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	83	3.7	0.216942	16	6.10471	13.4	8.1	3.5	88.4	16.3	0.211564
2	4.55	419	18.6	0.180673	13	3.77324	8.3	5	4.55	416.9	12.7	0.167422
3	5.2	611	27.1	0.253274	12	4.9978	11	6.6	5.2	608.4	11.6	0.243256
4	5.85	921	40.9	0.362532	9	5.48558	12.1	7.3	5.85	921.3	8.6	0.359212
5	6.55	1259	55.9	0.194629	11	3.51741	7.7	4.7	6.55	1258.2	11.2	0.177936
6	6.85	1347	59.8	0.169809	11	3.12511	6.9	4.1	6.85	1346	11.3	0.156249
7	7.35	1522	67.6	0.24905	11	4.71055	10.4	6.2	7.35	1519.7	10.9	0.244667
8	8.15	1810	80.3	0.105625	18	3.29949	7.3	4.4	8.15	1809.4	17.7	0.105135
9	8.45	1914	85	0.209081	12	4.40754	9.7	5.8	8.45	1913.6	12.2	0.20453
10	8.65	1978	87.8	0.18039	10	3.14534	6.9	4.2	8.65	1977.6	9.9	0.178556
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2222	98.6	0.183882	9	2.88326	6.3	3.8	9.3	2220.8	9.5	0.172118

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.779	480	21.3	0.0372661	11	0.745211	2	1.3	4.779	483.7	10.6	0.0397263
2	5.342	672	29.8	0.047246	8	0.701125	1.9	1.3	5.342	670.6	8.5	0.0466247
3	5.573	784	34.7	0.0594197	7	0.661583	1.8	1.2	5.573	785.5	7.1	0.0523003
4	5.688	840	37.2	0.173267	5	1.64719	4.5	2.9	5.688	839.7	5.4	0.171377
5	6.033	1006	44.6	0.067068	10	1.05861	2.9	1.9	6.033	1002.4	10.1	0.0594008
6	6.088	1032	45.7	0.0850785	4	0.55629	1.5	1	6.088	1031.9	3.8	0.0834024
7	6.129	1052	46.6	0.426124	6	4.02907	11.1	7.2	6.129	1052.3	5.5	0.409943
8	6.23	1100	48.7	0.201395	7	2.43172	6.7	4.4	6.23	1100.2	7.2	0.190585
9	6.28	1124	49.8	0.0500942	7	0.593234	1.6	1.1	6.28	1123.6	7.1	0.0468559
10	6.357	1161	51.4	0.0643633	7	0.727457	2	1.3	6.357	1160.5	6.9	0.0595794
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.383	1173	51.9	0.149008	5	1.19986	3.3	2.1	6.383	1173.9	4.7	0.143288
12	6.447	1204	53.3	0.0777005	5	0.639868	1.8	1.1	6.447	1203.2	4.7	0.0774649
13	6.527	1242	55	0.110762	5	0.900079	2.5	1.6	6.527	1242.9	5.3	0.0953949
14	6.56	1256	55.6	0.366661	4	2.58604	7.1	4.6	6.56	1256.2	4.2	0.347472
15	6.622	1274	56.4	0.0961769	6	0.854887	2.4	1.5	6.622	1274.2	5.8	0.0832973
16	6.736	1307	57.9	0.269608	10	5.0715	13.9	9.1	6.736	1310.2	10.5	0.273605
17	6.867	1346	59.6	0.104223	5	0.900851	2.5	1.6	6.867	1345.3	5.2	0.0969555
18	7.047	1410	62.4	0.192504	8	2.58588	7.1	4.6	7.047	1409.6	7.7	0.190664
19	7.154	1448	64.1	0.402972	5	3.89677	10.7	7	7.154	1447.5	5.3	0.416352
20	7.742	1659	73.5	0.0244245	5	0.355907	1	0.6	7.742	1655.7	4.7	0.0429222
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.833	1692	74.9	0.304827	8	4.21661	11.6	7.5	7.833	1690.4	7.9	0.300592

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	87	3.9	0.246904	23	9.66641	20	12.1	3.5	95.5	23	0.237414
2	4.55	407	18	0.295595	8	3.81025	7.9	4.8	4.55	407.6	8	0.270403
3	5.2	595	26.4	0.375515	8	5.28347	10.9	6.6	5.2	594.1	8.3	0.357243
4	5.85	916	40.6	0.401528	8	5.47939	11.3	6.8	5.85	917.5	7.7	0.40349
5	6.55	1247	55.3	0.176921	12	3.33466	6.9	4.2	6.55	1245.4	11.6	0.162465
6	6.85	1333	59.1	0.182118	12	3.50423	7.2	4.4	6.85	1333	12	0.164805
7	7.35	1513	67	0.25132	10	4.29516	8.9	5.4	7.35	1510.8	10	0.242654
8	8.15	1803	79.9	0.121122	18	3.78233	7.8	4.7	8.15	1800.6	17.6	0.121335
9	8.45	1903	84.3	0.219035	11	4.36029	9	5.4	8.45	1903.5	11.3	0.218027
10	8.65	1968	87.2	0.178083	9	2.92134	6	3.6	8.65	1967.5	9.3	0.176785
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2216	98.2	0.15055	7	1.93602	4	2.4	9.3	2214.7	7.3	0.149028

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	86	3.8	0.236331	20	7.8336	9.1	6.4	3.5	90.7	19.8	0.223255
2	4.55	402	17.8	0.144369	13	3.25192	3.8	2.7	4.55	406.4	13.1	0.139961
3	4.77	465	20.6	0.0389095	16	1.06987	1.2	0.9	4.77	462.6	15.5	0.0388378
4	5.2	588	26.1	0.162475	18	4.60728	5.3	3.8	5.2	587.9	17.5	0.1484
5	5.329	652	28.9	0.0448781	16	1.32107	1.5	1.1	5.329	648.4	16.2	0.0459755
6	5.571	772	34.3	0.045615	17	1.3059	1.5	1.1	5.571	773	16.9	0.043692
7	5.689	830	36.8	0.103981	12	2.0281	2.3	1.7	5.689	830	11.6	0.0988043
8	5.85	910	40.4	0.267728	9	4.24488	4.9	3.5	5.85	909.7	9.4	0.256001
9	6.045	1002	44.5	0.0724219	13	1.60092	1.9	1.3	6.045	1000.2	13.2	0.0685973
10	6.1	1028	45.6	0.09954	6	0.944392	1.1	0.8	6.1	1027.4	5.7	0.0932909
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.147	1050	46.6	0.422019	7	4.55395	5.3	3.7	6.147	1051.1	6.6	0.387174
12	6.249	1098	48.7	0.188988	8	2.54734	2.9	2.1	6.249	1098.8	8	0.179449
13	6.308	1126	50	0.0576239	8	0.721979	0.8	0.6	6.308	1125.1	7.8	0.0525133
14	6.393	1166	51.8	0.0908638	7	0.99603	1.2	0.8	6.393	1165.7	6.9	0.0819994
15	6.418	1178	52.3	0.17597	5	1.35384	1.6	1.1	6.418	1178.6	4.9	0.156785
16	6.488	1211	53.8	0.0448877	16	1.75064	2	1.4	6.488	1199.1	15.9	0.0622178
17	6.55	1240	55	0.102139	6	1.08929	1.3	0.9	6.55	1237.6	6.4	0.0956889
18	6.596	1252	55.6	0.244878	7	2.50851	2.9	2.1	6.596	1252.4	6.5	0.216204
19	6.658	1268	56.3	0.404619	4	2.54627	2.9	2.1	6.658	1268.6	3.7	0.393179
20	6.735	1288	57.2	0.117994	3	0.638244	0.7	0.5	6.735	1288.8	3.1	0.116417
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.85	1318	58.5	0.28777	11	5.61016	6.5	4.6	6.85	1322.5	10.8	0.293107
22	6.967	1360	60.4	0.112701	5	0.883798	1	0.7	6.967	1359.5	4.8	0.103016
23	7.161	1430	63.5	0.224884	9	3.86308	4.5	3.2	7.161	1428.7	9.4	0.231715
24	7.25	1462	64.9	0.366758	5	3.40492	3.9	2.8	7.25	1462.5	5.2	0.372633
25	7.35	1498	66.5	0.210532	9	3.17736	3.7	2.6	7.35	1496.1	8.7	0.206691
26	7.832	1669	74.1	0.0201696	4	0.176116	0.2	0.1	7.832	1667.1	3.8	0.0262863
27	7.93	1704	75.6	0.308004	8	4.05664	4.7	3.3	7.93	1702.5	7.5	0.303678
28	8.15	1782	79.1	0.0754682	15	2.07543	2.4	1.7	8.15	1784.1	15.3	0.0766062
29	8.45	1906	84.6	0.204805	36	11.5923	13.4	9.5	8.45	1904.8	36	0.181678
30	8.65	1968	87.4	0.157927	9	2.17517	2.5	1.8	8.65	1967.7	8.6	0.142383
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.3	2206	97.9	0.179814	8	2.56923	3	2.1	9.3	2204	8.4	0.171969

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.793	481	21.3	0.0353411	15	0.907249	1.8	1.3	4.793	482.7	15.4	0.0332101
2	5.343	668	29.5	0.0414043	15	1.04402	2.1	1.5	5.343	665.4	15	0.0393803
3	5.576	784	34.7	0.041206	14	0.973253	2	1.4	5.576	789.1	13.8	0.0398174
4	5.7	846	37.4	0.155927	8	1.98227	4	2.8	5.7	846.4	7.7	0.145332
5	6.041	1012	44.7	0.0774719	13	1.45666	2.9	2	6.041	1011.2	12.5	0.0655745
6	6.091	1036	45.8	0.107901	6	1.00096	2	1.4	6.091	1036.6	5.8	0.0971107
7	6.137	1058	46.8	0.463154	6	4.51447	9.1	6.3	6.137	1059.3	5.9	0.430541
8	6.238	1106	48.9	0.212473	7	2.58322	5.2	3.6	6.238	1106.9	7.2	0.20175
9	6.292	1132	50	0.056475	7	0.621438	1.3	0.9	6.292	1131.6	6.8	0.0512507
10	6.372	1170	51.7	0.0701941	5	0.638527	1.3	0.9	6.372	1169.9	5.4	0.0666774
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.397	1182	52.3	0.154342	5	1.28106	2.6	1.8	6.397	1182.9	5	0.143988
12	6.456	1210	53.5	0.0688278	5	0.628304	1.3	0.9	6.456	1209.8	5.1	0.0693575
13	6.54	1250	55.3	0.255474	8	3.20566	6.5	4.5	6.54	1249.9	8.2	0.219838
14	6.59	1266	56	0.32091	4	2.53585	5.1	3.6	6.59	1264.8	4.1	0.348643
15	6.645	1281	56.6	0.0707583	4	0.723759	1.5	1	6.645	1283.3	3.7	0.109315
16	6.77	1315	58.1	0.289809	13	7.12304	14.4	10	6.77	1317.5	13.3	0.302705
17	6.897	1354	59.9	0.144899	10	2.21391	4.5	3.1	6.897	1355.4	10.4	0.119806
18	7.079	1420	62.8	0.222736	8	2.91618	5.9	4.1	7.079	1418.6	7.6	0.216796
19	7.184	1458	64.5	0.443584	6	4.53183	9.2	6.4	7.184	1458	5.6	0.454408
20	7.758	1666	73.7	0.0955453	20	4.04103	8.2	5.7	7.758	1651.5	19.6	0.11632
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.858	1702	75.2	0.337686	8	4.49471	9.1	6.3	7.858	1700.6	7.6	0.33162

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	99	4.4	0.212544	31	10.8505	22.1	14.5	3.5	103.2	31	0.197691
2	4.55	404	17.9	0.271423	8	3.7539	7.7	5	4.55	404.7	8.2	0.258009
3	5.2	587	26	0.360245	9	5.63705	11.5	7.5	5.2	586.5	9.2	0.347153
4	5.85	914	40.5	0.41208	8	5.62453	11.5	7.5	5.85	914.9	7.7	0.410808
5	6.55	1252	55.5	0.237077	11	4.29744	8.8	5.7	6.55	1249.7	11	0.220455
6	6.85	1338	59.3	0.195971	11	3.54706	7.2	4.7	6.85	1337.1	10.9	0.184003
7	7.35	1520	67.4	0.239558	11	4.34869	8.9	5.8	7.35	1518.4	10.7	0.22946
8	8.15	1816	80.5	0.0975079	16	2.71739	5.5	3.6	8.15	1812.9	16.3	0.0940313
9	8.45	1918	85	0.209677	10	3.80954	7.8	5.1	8.45	1917.1	10.4	0.205748
10	8.65	1982	87.9	0.170376	9	2.60114	5.3	3.5	8.65	1980.9	8.8	0.166117
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2217	98.3	0.128581	9	1.85836	3.8	2.5	9.3	2214.5	8.8	0.118703

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 106

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 13.400 4.550 8.300 5.200 11.000 5.850 12.100 6.550 7.700 6.850 6.900 7.350 10.400 8.150 7.300 8.450 9.700 8.650 6.900 9.300 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
4.779 2.000 5.342 1.900 5.573 1.800 5.688 4.500 6.033 2.900 6.088 1.500 6.129 11.100 6.230 6.700 6.280 1.600 6.357 2.000 6.383 3.300 6.447 1.800 6.527 2.500 6.560 7.100 6.622 2.400 6.736 13.900 6.867 2.500 7.047 7.100 7.154 10.700 7.742 1.000 7.833 11.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 21 Return to Lane Navigator
3.500 20.000 4.550 7.900 5.200 10.900 5.850 11.300 6.550 6.900 6.850 7.200 7.350 8.900 8.150 7.800 8.450 9.000 8.650 6.000 9.300 4.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 9.100 4.550 3.800 4.770 1.200 5.200 5.300 5.329 1.500 5.571 1.500 5.689 2.300 5.850 4.900 6.045 1.900 6.100 1.100 6.147 5.300 6.249 2.900 6.308 0.800 6.393 1.200 6.418 1.600 6.488 2.000 6.550 1.300 6.596 2.900 6.658 2.900 6.735 0.700 6.850 6.500 6.967 1.000 7.161 4.500 7.250 3.900 7.350 3.700 7.832 0.200 7.930 4.700 8.150 2.400 8.450 13.400 8.650 2.500 9.300 3.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 31 Return to Lane Navigator
4.793 1.800 5.343 2.100 5.576 2.000 5.700 4.000 6.041 2.900 6.091 2.000 6.137 9.100 6.238 5.200 6.292 1.300 6.372 1.300 6.397 2.600 6.456 1.300 6.540 6.500 6.590 5.100 6.645 1.500 6.770 14.400 6.897 4.500 7.079 5.900 7.184 9.200 7.758 8.200 7.858 9.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 21 Return to Lane Navigator
3.500 22.100 4.550 7.700 5.200 11.500 5.850 11.500 6.550 8.800 6.850 7.200 7.350 8.900 8.150 5.500 8.450 7.800 8.650 5.300 9.300 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Escolar(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.