RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Channel Catfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ictalurus punctatus
FDA Seafood List Common Name: Channel Catfish
FDA Seafood List Market Name: Catfish
Unique IEF Gel Code: 19970218gel04
Unique Web Code: cch02Srr
Tissue Aliquot Code: ictapunc K95-002 #09p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	111	13	0.0784973	3	0.452409	5.7	4.1	4.55	111.1	3.3	0.0773394
2	5.2	202	23.7	0.0993401	4	0.677061	8.6	6.2	5.2	202.7	3.9	0.0970186
3	5.85	306	35.9	0.171858	3	1.02075	12.9	9.3	5.85	306	3.4	0.168772
4	6.55	444	52.1	0.107665	7	1.2276	15.5	11.2	6.55	442.6	6.9	0.100958
5	6.85	475	55.7	0.0553756	5	0.436006	5.5	4	6.85	476.2	4.8	0.0513318
6	7.35	540	63.3	0.0936568	4	0.602192	7.6	5.5	7.35	539.6	3.6	0.0935621
7	8.15	659	77.3	0.107831	6	1.15178	14.6	10.5	8.15	658.7	6.2	0.10547
8	8.45	721	84.5	0.128162	6	1.36242	17.3	12.4	8.45	721	6.1	0.126088
9	8.65	776	91	0.092511	4	0.638713	8.1	5.8	8.65	776	3.9	0.0926453
10	9.3	834	97.8	0.0475618	4	0.328083	4.2	3	9.3	833.7	3.9	0.0470464

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.921	23	2.7	0.129299	6	1.38172	4.8	3.3	3.921	22	6.3	0.12354
2	4.263	72	8.4	0.359794	4	2.76247	9.6	6.6	4.263	72	4.3	0.359041
3	4.515	108	12.6	0.36203	4	2.57956	9	6.2	4.515	107.8	4	0.363079
4	4.753	142	16.6	0.280001	4	1.83431	6.4	4.4	4.753	141.9	3.7	0.281339
5	4.92	166	19.4	0.0451271	9	0.670818	2.3	1.6	4.92	165.7	8.5	0.0443014
6	5.025	181	21.1	0.334953	2	1.34555	4.7	3.2	5.025	181.2	2.3	0.327738
7	5.193	205	23.9	0.0695394	3	0.289586	1	0.7	5.193	204.8	2.6	0.0626713
8	5.251	214	25	0.037221	7	0.446787	1.6	1.1	5.251	214.8	6.8	0.0369325
9	5.633	274	32	0.0204365	2	0.081955	0.3	0.2	5.633	274	2.3	0.0197378
10	5.703	285	33.3	0.120712	3	0.637891	2.2	1.5	5.703	285.7	3	0.121106
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.767	295	34.4	0.0713235	3	0.349826	1.2	0.8	5.767	294.6	2.9	0.0685815
12	5.831	305	35.6	0.439421	3	2.31268	8.1	5.6	5.831	305.6	3	0.430903
13	5.905	319	37.2	0.322119	4	2.4097	8.4	5.8	5.905	318.8	4.4	0.306151
14	5.975	333	38.9	0.0747808	6	0.776403	2.7	1.9	5.975	332.9	5.8	0.0761261
15	6.046	347	40.5	0.0552133	2	0.201926	0.7	0.5	6.046	347.4	2.2	0.0529602
16	6.186	375	43.8	0.0655358	2	0.229711	0.8	0.6	6.186	375.3	1.9	0.066794
17	6.216	381	44.5	0.0508032	2	0.204395	0.7	0.5	6.216	380.3	2.1	0.0546839
18	6.292	396	46.2	0.0644967	3	0.319917	1.1	0.8	6.292	395.4	3	0.0607423
19	6.337	405	47.3	0.13578	3	0.60444	2.1	1.5	6.337	404.8	2.6	0.131896
20	6.377	413	48.2	0.0393356	4	0.257663	0.9	0.6	6.377	413.1	3.7	0.0397178
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.422	422	49.2	0.276443	2	0.772448	2.7	1.9	6.422	421.7	1.6	0.281149
22	6.467	431	50.3	0.422141	2	1.74684	6.1	4.2	6.467	431.3	2.3	0.430322
23	6.874	482	56.2	0.0297841	4	0.161678	0.6	0.4	6.874	481.1	3.5	0.0259626
24	6.93	489	57.1	0.291639	2	1.14833	4	2.8	6.93	489.1	2.3	0.284167
25	7.29	534	62.3	0.0747864	5	0.568347	2	1.4	7.29	533.8	4.8	0.0661401
26	7.413	551	64.3	0.0265482	4	0.166605	0.6	0.4	7.413	550.1	4	0.0233848
27	8.746	787	91.8	0.147855	18	4.43272	15.4	10.7	8.746	784.6	18	0.138941

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	109	12.7	0.0293806	6	0.294747	3.3	2.3	4.55	108.2	6	0.027686
2	5.2	204	23.9	0.0468846	9	0.799494	9	6.3	5.2	200.6	9.5	0.0476017
3	5.85	304	35.6	0.128842	5	1.14793	12.9	9.1	5.85	303.9	5	0.128981
4	6.55	445	52	0.120211	8	1.5093	16.9	12	6.55	444.6	7.6	0.112013
5	6.85	477	55.8	0.0628939	5	0.521974	5.8	4.1	6.85	477.1	5.1	0.0574471
6	7.35	537	62.8	0.111816	4	0.736582	8.2	5.8	7.35	537.1	3.8	0.110325
7	8.15	660	77.2	0.111158	7	1.26104	14.1	10	8.15	659.6	6.7	0.106924
8	8.45	722	84.4	0.116208	7	1.30994	14.7	10.4	8.45	721.4	6.5	0.113271
9	8.65	775	90.6	0.0950152	4	0.686627	7.7	5.5	8.65	774.8	4.3	0.0903712
10	9.3	832	97.3	0.0879344	4	0.662029	7.4	5.3	9.3	831.7	4.3	0.0870363

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.929	24	2.8	0.0782711	5	0.728478	3	2.4	3.929	23.7	5.4	0.0757293
2	4.236	67	7.8	0.242576	5	2.24528	9.2	7.4	4.236	67.1	5.1	0.246092
3	4.486	102	11.9	0.236287	5	2.03203	8.3	6.7	4.486	101.9	4.9	0.235951
4	4.55	111	12.9	0.0318924	5	0.262844	1.1	0.9	4.55	113.3	5.4	0.0273059
5	4.743	138	16.1	0.168027	5	1.47788	6	4.9	4.743	137.6	5	0.168284
6	4.9	160	18.6	0.0385068	6	0.451837	1.8	1.5	4.9	160.1	6.4	0.0400086
7	5.021	177	20.6	0.127263	5	1.10186	4.5	3.6	5.021	176.1	4.6	0.135786
8	5.2	202	23.5	0.0482856	8	0.676218	2.8	2.2	5.2	201.1	8.3	0.0458768
9	5.599	267	31.1	0.0143342	6	0.136641	0.6	0.5	5.599	267.7	5.7	0.0135689
10	5.715	286	33.3	0.0798904	10	1.28817	5.3	4.2	5.715	285.5	9.6	0.0756961
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.764	294	34.3	0.0425485	2	0.0565525	0.2	0.2	5.764	296	2	0.0159614
12	5.85	308	35.9	0.278784	5	2.45935	10.1	8.1	5.85	306.8	5.1	0.269676
13	5.917	321	37.4	0.24234	6	2.39921	9.8	7.9	5.917	320.4	5.5	0.244416
14	5.985	334	38.9	0.0475724	5	0.435728	1.8	1.4	5.985	332.2	4.5	0.054232
15	6.057	348	40.6	0.015073	3	0.0698161	0.3	0.2	6.057	347.4	2.7	0.0143378
16	6.192	374	43.6	0.0507519	3	0.185924	0.8	0.6	6.192	373.2	2.7	0.038376
17	6.213	378	44.1	0.0442166	5	0.299158	1.2	1	6.213	378.7	4.7	0.0357605
18	6.291	393	45.8	0.0427114	5	0.314492	1.3	1	6.291	391.4	4.6	0.0383229
19	6.337	402	46.9	0.0969047	2	0.389284	1.6	1.3	6.337	402	2.3	0.0943634
20	6.374	409	47.7	0.0335227	4	0.247835	1	0.8	6.374	410.5	4.4	0.0314898
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.426	419	48.8	0.227538	2	0.66758	2.7	2.2	6.426	419.3	1.6	0.231089
22	6.477	429	50	0.373058	2	1.31491	5.4	4.3	6.477	429	2	0.370853
23	6.55	443	51.6	0.0102161	3	0.0520099	0.2	0.2	6.55	442.6	2.8	0.0105225
24	6.85	477	55.6	0.046692	6	0.478717	2	1.6	6.85	477.3	6.1	0.0442095
25	6.94	488	56.9	0.210191	2	0.81077	3.3	2.7	6.94	488	2.2	0.209687
26	7.35	538	62.7	0.0799064	5	0.668026	2.7	2.2	7.35	537.8	4.7	0.0801604
27	7.427	550	64.1	0.0271716	4	0.170526	0.7	0.6	7.427	549.6	3.9	0.0247225
28	8.15	662	77.2	0.0425382	9	0.713199	2.9	2.4	8.15	661.9	9	0.0446923
29	8.45	727	84.7	0.0576174	5	0.535505	2.2	1.8	8.45	726.7	5.3	0.0567154
30	8.65	779	90.8	0.0886388	10	1.33477	5.5	4.4	8.65	780.1	10.2	0.0736509
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.3	836	97.4	0.0660198	4	0.460615	1.9	1.5	9.3	836	4	0.0648709

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.946	22	2.6	0.121471	5	1.16775	4.5	3.3	3.946	22.3	5.5	0.1202
2	4.259	66	7.7	0.334212	5	2.77716	10.7	7.9	4.259	66.2	4.7	0.330589
3	4.5	100	11.7	0.320861	4	2.55181	9.8	7.3	4.5	100.3	4.5	0.321427
4	4.756	136	15.9	0.2351	4	1.81584	7	5.2	4.756	135.8	4.4	0.23371
5	4.934	161	18.8	0.0449433	8	0.597275	2.3	1.7	4.934	161	7.8	0.0432209
6	5.04	176	20.6	0.229047	3	1.3335	5.1	3.8	5.04	175.3	3.2	0.234448
7	5.203	199	23.2	0.0475784	4	0.268048	1	0.8	5.203	198.4	3.5	0.0430539
8	5.264	209	24.4	0.032981	7	0.408789	1.6	1.2	5.264	208.9	7.5	0.0307909
9	5.636	270	31.5	0.0152213	2	0.0572658	0.2	0.2	5.636	270.2	2.1	0.0153105
10	5.71	282	32.9	0.109723	3	0.570641	2.2	1.6	5.71	282.4	2.9	0.109535
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.771	292	34.1	0.0593779	3	0.357989	1.4	1	5.771	291.6	3.5	0.0582387
12	5.832	302	35.3	0.377547	3	2.06146	7.9	5.9	5.832	302.8	3.1	0.38108
13	5.907	316	36.9	0.2862	5	2.2391	8.6	6.4	5.907	316.1	4.6	0.27274
14	5.979	330	38.6	0.0727288	5	0.64895	2.5	1.8	5.979	329.6	4.9	0.074606
15	6.046	343	40.1	0.0357677	2	0.130384	0.5	0.4	6.046	342.9	2.1	0.0349582
16	6.179	369	43.1	0.0485684	2	0.215525	0.8	0.6	6.179	369.4	2.5	0.0494685
17	6.205	374	43.7	0.0418459	2	0.162272	0.6	0.5	6.205	374.6	2.2	0.0424582
18	6.282	389	45.4	0.0461542	3	0.237836	0.9	0.7	6.282	389.1	3.1	0.04399
19	6.329	398	46.5	0.1115	2	0.489898	1.9	1.4	6.329	398.4	2.4	0.112972
20	6.375	407	47.5	0.0271232	3	0.181882	0.7	0.5	6.375	406.4	3.4	0.0304719
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.416	415	48.5	0.2487	2	0.7136	2.7	2	6.416	415	1.6	0.247881
22	6.463	424	49.5	0.40159	2	1.56988	6	4.5	6.463	424.3	2.2	0.40362
23	6.85	474	55.4	0.0221253	3	0.115032	0.4	0.3	6.85	474.3	3.3	0.0195706
24	6.912	482	56.3	0.262969	2	0.989283	3.8	2.8	6.912	482.3	2.1	0.267382
25	7.311	533	62.3	0.0817306	6	0.712683	2.7	2	7.311	532.1	5.8	0.0698743
26	7.43	550	64.3	0.0241356	5	0.215538	0.8	0.6	7.43	547.8	5.5	0.0222495
27	8.718	782	91.4	0.118962	18	3.44564	13.2	9.8	8.718	782.9	18	0.108002

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	107	12.5	0.0537464	4	0.412436	6.1	4.4	4.55	107	4.3	0.0535411
2	5.2	199	23.2	0.0689847	5	0.565023	8.4	6	5.2	198.8	4.8	0.0665672
3	5.85	306	35.7	0.150605	4	0.950552	14.1	10.1	5.85	306.2	3.6	0.148165
4	6.55	443	51.6	0.0882629	8	1.09859	16.3	11.6	6.55	441.5	7.6	0.0817793
5	6.85	475	55.4	0.0323751	4	0.227234	3.4	2.4	6.85	475.2	4.2	0.0304502
6	7.35	542	63.2	0.0683548	4	0.44338	6.6	4.7	7.35	541.6	3.8	0.0660154
7	8.15	659	76.8	0.0874926	6	0.966507	14.4	10.2	8.15	659.4	6.5	0.0842964
8	8.45	721	84	0.0900749	7	1.04195	15.5	11	8.45	720.4	6.8	0.0864358
9	8.65	777	90.6	0.0807483	4	0.604333	9	6.4	8.65	776.3	4.5	0.0759082
10	9.3	834	97.2	0.0543121	4	0.424677	6.3	4.5	9.3	833.9	4.5	0.0534167

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 115

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.700 5.200 8.600 5.850 12.900 6.550 15.500 6.850 5.500 7.350 7.600 8.150 14.600 8.450 17.300 8.650 8.100 9.300 4.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.921 4.800 4.263 9.600 4.515 9.000 4.753 6.400 4.920 2.300 5.025 4.700 5.193 1.000 5.251 1.600 5.633 0.300 5.703 2.200 5.767 1.200 5.831 8.100 5.905 8.400 5.975 2.700 6.046 0.700 6.186 0.800 6.216 0.700 6.292 1.100 6.337 2.100 6.377 0.900 6.422 2.700 6.467 6.100 6.874 0.600 6.930 4.000 7.290 2.000 7.413 0.600 8.746 15.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 27 Return to Lane Navigator
4.550 3.300 5.200 9.000 5.850 12.900 6.550 16.900 6.850 5.800 7.350 8.200 8.150 14.100 8.450 14.700 8.650 7.700 9.300 7.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.929 3.000 4.236 9.200 4.486 8.300 4.550 1.100 4.743 6.000 4.900 1.800 5.021 4.500 5.200 2.800 5.599 0.600 5.715 5.300 5.764 0.200 5.850 10.100 5.917 9.800 5.985 1.800 6.057 0.300 6.192 0.800 6.213 1.200 6.291 1.300 6.337 1.600 6.374 1.000 6.426 2.700 6.477 5.400 6.550 0.200 6.850 2.000 6.940 3.300 7.350 2.700 7.427 0.700 8.150 2.900 8.450 2.200 8.650 5.500 9.300 1.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 31 Return to Lane Navigator
3.946 4.500 4.259 10.700 4.500 9.800 4.756 7.000 4.934 2.300 5.040 5.100 5.203 1.000 5.264 1.600 5.636 0.200 5.710 2.200 5.771 1.400 5.832 7.900 5.907 8.600 5.979 2.500 6.046 0.500 6.179 0.800 6.205 0.600 6.282 0.900 6.329 1.900 6.375 0.700 6.416 2.700 6.463 6.000 6.850 0.400 6.912 3.800 7.311 2.700 7.430 0.800 8.718 13.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 27 Return to Lane Navigator
4.550 6.100 5.200 8.400 5.850 14.100 6.550 16.300 6.850 3.400 7.350 6.600 8.150 14.400 8.450 15.500 8.650 9.000 9.300 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Channel Catfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Channel Catfish ---- Go to Display Gel Lane Navigator.