Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Sebastes pinniger
FDA Seafood List Common Name: Canary Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940908gel04
Unique Web Code: cag01Srr
Tissue Aliquot Code: sebapinn I94-002 #09a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel ImageCalibrated Comparison Gel Image
Gel ImageFish ImageCalibrated GelComparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
Co-Migrated
Lane d
pI Markers
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5494.40.241129145.7051816.613.43.550.414.20.226838
24.5520518.50.31996852.717417.96.44.55205.55.30.290881
35.229226.30.45339953.7006210.88.75.2292.34.70.446198
45.8545040.50.56900243.6685510.78.65.85450.73.60.582133
56.5561655.40.43877343.140459.27.46.55615.94.20.426164
66.85656590.12355561.20773.52.86.85655.76.10.110851
77.35744670.43079753.5202710.38.37.35743.64.60.42855
88.1589380.40.0914038101.479124.33.58.15891.79.80.0850945
98.45955860.13664371.70063548.45955.27.40.130345
108.6599389.40.10519350.9789042.92.38.65992.15.50.10071
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3109998.90.49447486.4505218.815.29.31097.27.80.469381
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.18415213.70.48804154.5761212.110.64.1841535.30.482901
24.49219717.70.028477480.3450260.90.84.492194.580.0243652
34.72622920.60.50303765.2030513.7124.726229.25.90.50091
45.03927124.40.041230830.07210340.20.25.039270.72.90.0139813
55.12228225.40.115756154.5099511.910.45.122292.614.60.173765
65.73342037.80.057442590.8756942.325.733418.88.80.0561584
75.77943138.80.026431130.1084440.30.35.779432.63.20.0191924
85.884456410.35879944.16851119.65.884453.64.10.572239
95.96447542.80.44894264.601312.110.65.964474.96.10.427519
106.10750945.80.1927241.082182.92.56.107509.33.60.168032
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.14151746.50.026686310006.14150110
126.16652347.10.63853222.316416.15.46.166523.51.90.679365
136.29755449.90.014516560.1518460.40.46.297554.16.10.0139529
146.37757351.60.032942940.2300270.60.56.377572.540.0325223
156.4458852.90.0083541130.04553990.10.16.44588.53.20.00810687
166.48359853.80.050204820.1656660.40.46.4835982.10.0440094
176.53361054.90.054921470.5785451.51.36.533607.86.90.0475034
187.375744670.0457091150.9225612.42.17.37574514.70.0355171
197.55177669.80.016147350.1279680.30.37.551775.55.20.0139663
207.65579571.60.10645450.8572912.327.655795.24.80.101637
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.40594184.70.177782237.0133118.516.28.40593622.80.173551
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.55650.16332184.867947.873.556.6180.152583
24.1414813.20.49573654.169446.664.141484.60.508656
34.43919117.10.0375340.2591540.40.44.439191.33.90.0372525
44.5520718.50.20106941.451292.32.14.55207.54.30.1917
54.66222219.80.55062754.76977.66.84.6622224.80.562586
64.96826323.50.059313220.1590720.30.24.968262.32.20.0416299
75.03627224.30.108219154.129136.65.95.036283.614.70.158086
85.229426.30.31108941.414062.325.2293.73.70.214281
95.71441537.10.092878191.330632.11.95.714413.390.0831634
105.8544739.90.57689154.610717.36.65.85447.84.70.55276
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.95547242.20.39223774.585867.36.65.955471.56.70.386138
126.04449344.10.0204520.08058930.10.16.044493.82.30.0196797
136.10750845.40.1759730.8804721.41.36.107507.82.90.170267
146.12851345.80.080297620.2748840.40.46.128513.81.60.0946714
156.1752346.70.67113522.653344.23.86.175232.10.710041
166.30155449.50.020966850.1767240.30.36.301553.94.80.0208295
176.38657451.30.046469260.4291860.70.66.386575.460.0404115
186.45759152.80.0800357101.637472.62.36.4575959.60.095976
196.50460253.80.27235451.789482.92.66.504602.84.90.207524
206.5561354.80.095089840.5937160.90.86.55612.93.90.0865814
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.85649580.099026850.8067991.31.26.85648.45.30.0858209
227.3573665.80.40119753.429815.54.97.35735.64.80.402575
237.43575167.10.048053350.4077290.60.67.435750.45.10.0449578
247.615783700.013643440.09195760.10.17.6157824.10.0128063
257.72280271.70.12560361.2274921.87.722801.95.70.120919
268.17188378.90.094046742.234353.63.28.171878.63.50.355161
278.4594884.70.245164176.686210.79.68.45948.816.60.227504
288.6599088.50.08673140.5457190.90.88.65990.23.90.0782027
299.3109998.20.48368697.0665411.310.19.31097.38.60.461144
PeakCounter: 29
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5544.80.206456196.7201418.815.73.557.919.20.197531
24.5520918.60.25956772.786787.86.54.55209.56.70.234991
35.229626.40.32467863.57133108.35.2296.26.40.312977
45.8545440.40.42921843.367979.47.95.854544.40.432886
56.5561054.30.39527753.374249.57.96.55609.550.379252
66.8565057.90.12503171.360373.83.26.85649.86.70.113828
77.3573765.60.39949653.522119.98.27.35736.750.400772
88.1588879.10.0954593101.645084.63.88.15886.910.30.0898264
98.4595184.70.14247371.696924.848.45950.870.137017
108.6598988.10.10841150.9573152.72.28.65989.150.106983
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3110398.20.47268696.6631218.715.69.31100.78.50.441779
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.14214913.40.52624944.2291711.510.24.142149.24.40.538244
24.44919317.40.042264570.4556181.21.14.449190.97.10.0362832
34.68322520.20.57449755.0556313.712.24.683224.44.80.590701
45.002267240.048002720.09413380.30.25.002265.31.80.0296277
55.07127624.80.127893143.9929710.89.65.071283.913.50.166445
65.70441637.40.0780716111.383193.73.35.704415.410.80.0720576
75.8444940.40.61931544.5370612.3115.84449.44.30.591898
85.94147242.40.42759864.1808911.310.15.941471.55.70.416803
95.99448443.50.076094440.5093061.41.25.994484.34.50.0645018
106.09250645.50.17392541.025412.82.56.092506.23.90.1487
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.12351346.10.071929210006.12349710
126.15952146.90.62624222.439436.65.96.159520.72.10.664524
136.37256951.20.03002340.2278520.60.66.372569.64.20.0304138
146.45258752.80.035833240.24070.70.66.452587.43.90.0343828
156.48859553.50.099902830.4592591.21.16.488595.12.90.090564
166.53760654.50.088507550.7174451.91.76.537605.85.20.0780792
177.37474166.60.015701930.07550760.20.27.374740.22.80.0153664
187.54577369.50.0064151630.05008910.10.17.545771.72.80.00994462
197.635790710.099115240.6527541.81.67.635790.13.70.100702
208.42294484.90.19154196.5590417.815.88.422945.119.20.192551
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5645.70.205828227.6413821.817.73.565.921.70.198787
24.5521018.70.23734872.845398.16.64.55209.870.230882
35.229426.10.30550873.6310910.48.45.2294.36.60.31044
45.8545340.20.49201243.390539.77.95.85453.13.90.496592
56.5561254.40.40311643.002538.676.556124.40.385375
66.8565257.90.11511171.179753.42.76.85651.86.60.101576
77.3574065.70.39401653.190519.17.47.35739.34.60.389114
88.15889790.091144111.667234.83.98.15888.5110.0854977
98.4595384.60.13924161.529484.43.58.45952.76.40.135158
108.65991880.11052950.9761162.82.38.65990.650.109793
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3110998.50.44982385.985217.113.99.311078.10.417011
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP CO MK SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
PeakCount: 29 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln