RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Brown Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes auriculatus
FDA Seafood List Common Name: Brown Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19980327gel01
Unique Web Code: bnh01Srr
Tissue Aliquot Code: sebaauri 002 #3 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	61	7.9	0.102353	4	0.663114	7.3	5.7	4.55	61.8	3.8	0.0992107
2	5.2	155	20.2	0.107667	4	0.790735	8.7	6.8	5.2	155.1	4.2	0.105336
3	5.85	266	34.6	0.0628652	7	0.850986	9.4	7.4	5.85	267.3	7.5	0.0644369
4	6.55	401	52.1	0.11992	8	1.61924	17.9	14	6.55	399.6	7.7	0.118949
5	6.85	440	57.2	0.0337618	8	0.398799	4.4	3.5	6.85	438	7.7	0.0291806
6	7.35	504	65.5	0.0765575	5	0.757619	8.4	6.6	7.35	503.5	5.5	0.0779662
7	8.15	627	81.5	0.0750085	11	1.4038	15.5	12.2	8.15	625.9	11.3	0.0703735
8	8.45	690	89.7	0.0935887	10	1.68165	18.6	14.6	8.45	691.2	10.3	0.0925097
9	8.65	743	96.6	0.091609	6	0.889451	9.8	7.7	8.65	742.1	5.6	0.0891257

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.291	22	2.9	0.180043	7	2.04629	10.2	5.4	4.291	23.9	6.7	0.172332
2	4.507	52	6.8	0.191156	6	2.17039	10.8	5.8	4.507	52	6.4	0.190379
3	4.6	65	8.5	0.0674777	6	0.783182	3.9	2.1	4.6	62.9	6.5	0.0681059
4	4.737	84	10.9	0.159663	5	1.28252	6.4	3.4	4.737	84.6	4.6	0.155664
5	5.924	277	36.1	0.447069	4	3.58574	17.8	9.5	5.924	276.6	4.5	0.453746
6	5.996	291	37.9	0.275949	8	3.59903	17.9	9.6	5.996	292.1	7.6	0.267899
7	6.128	317	41.3	0.0894425	3	0.451585	2.2	1.2	6.128	317.1	3	0.0838897
8	6.164	324	42.2	0.105982	4	0.74747	3.7	2	6.164	324	4	0.104839
9	6.21	333	43.4	0.267117	2	1.03134	5.1	2.7	6.21	332.6	2.2	0.269515
10	6.328	356	46.4	0.106352	6	1.03349	5.1	2.7	6.328	357.2	5.6	0.103737
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.466	383	49.9	0.0923891	4	0.500181	2.5	1.3	6.466	381.8	3.9	0.0719909
12	6.512	392	51	0.073211	8	0.889268	4.4	2.4	6.512	390.8	7.8	0.0639222
13	7.366	504	65.6	0.073241	4	0.499925	2.5	1.3	7.366	504.8	4.2	0.0676812
14	7.599	540	70.3	0.153861	6	1.52696	7.6	4.1	7.599	541.1	5.9	0.145114

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	55	7.2	0.0566228	6	0.616495	7.8	5.7	4.55	56.5	6.1	0.0572114
2	5.2	142	18.5	0.0543364	8	0.796275	10	7.4	5.2	145.4	8.3	0.0538888
3	5.85	259	33.8	0.0659756	6	0.665357	8.4	6.2	5.85	258.5	5.8	0.064991
4	6.55	398	52	0.119789	7	1.39686	17.6	13	6.55	397.1	7.2	0.108903
5	6.85	433	56.5	0.0455886	5	0.357527	4.5	3.3	6.85	433.5	4.8	0.0417429
6	7.35	499	65.1	0.0868231	4	0.599564	7.6	5.6	7.35	498.5	4	0.0841076
7	8.15	623	81.3	0.098381	8	1.29263	16.3	12	8.15	623.3	7.8	0.0937629
8	8.45	682	89	0.110489	8	1.44648	18.3	13.4	8.45	681.6	7.5	0.108217
9	8.65	736	96.1	0.101012	4	0.753297	9.5	7	8.65	735.3	4.4	0.0963583

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.259	20	2.6	0.18975	7	2.19488	11.1	6.4	4.259	21.6	6.8	0.180864
2	4.482	53	6.9	0.144191	5	0.794801	4	2.3	4.482	52	4.9	0.0906911
3	4.55	63	8.2	0.0932989	9	1.56598	7.9	4.6	4.55	59.7	8.8	0.100289
4	4.692	84	11	0.139032	6	1.27601	6.4	3.7	4.692	85.2	5.8	0.124661
5	5.2	159	20.8	0.0285161	4	0.185149	0.9	0.5	5.2	159.3	3.6	0.0291293
6	5.85	263	34.4	0.0194147	5	0.172711	0.9	0.5	5.85	260.7	5.4	0.0181102
7	5.921	277	36.2	0.460586	4	3.36381	17	9.9	5.921	276.6	4.2	0.456565
8	5.977	288	37.6	0.289903	7	3.46792	17.5	10.2	5.977	290.4	7	0.279811
9	6.119	316	41.3	0.0744496	3	0.403931	2	1.2	6.119	316	3.2	0.0719269
10	6.164	325	42.5	0.0725294	4	0.553788	2.8	1.6	6.164	324.2	4.3	0.0720426
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.205	333	43.5	0.277423	2	0.895763	4.5	2.6	6.205	332.9	1.9	0.265432
12	6.332	358	46.8	0.10553	6	0.977988	4.9	2.9	6.332	358.4	5.7	0.0966322
13	6.464	384	50.2	0.111657	3	0.543382	2.7	1.6	6.464	383.1	3.5	0.0879307
14	6.504	392	51.2	0.0782664	7	0.931813	4.7	2.7	6.504	391.6	7.4	0.0710086
15	6.85	435	56.9	0.0244444	6	0.252026	1.3	0.7	6.85	434.6	6.2	0.0229109
16	7.35	505	66	0.0557631	4	0.378185	1.9	1.1	7.35	505	4.3	0.0497504
17	7.614	545	71.2	0.147632	5	1.17	5.9	3.4	7.614	544.9	4.6	0.143212
18	8.45	694	90.7	0.0345973	7	0.403864	2	1.2	8.45	692.6	6.8	0.0334907
19	8.65	743	97.1	0.0329644	5	0.26148	1.3	0.8	8.65	743.2	4.6	0.032029

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.249	21	2.8	0.174473	7	1.98535	8.2	5.9	4.249	22.8	6.7	0.166796
2	4.452	50	6.6	0.226268	5	1.67837	6.9	5	4.452	49.8	5	0.187601
3	4.55	64	8.4	0.0829997	9	1.71259	7.1	5.1	4.55	58.9	8.8	0.109984
4	4.683	83	10.9	0.218525	8	2.74714	11.3	8.2	4.683	85.4	8.1	0.191885
5	5.929	282	37	0.39137	6	4.08588	16.9	12.2	5.929	280.9	6.2	0.370733
6	6	296	38.8	0.267878	7	3.24511	13.4	9.7	6	296.7	7.1	0.2592
7	6.148	325	42.6	0.0952785	10	1.59549	6.6	4.8	6.148	323.8	10.2	0.0882221
8	6.209	337	44.2	0.16771	4	1.24067	5.1	3.7	6.209	337.1	4.3	0.162578
9	6.341	363	47.6	0.0737514	7	0.900458	3.7	2.7	6.341	364	6.9	0.0739127
10	6.499	394	51.6	0.0670823	11	1.2612	5.2	3.8	6.499	397.1	11.1	0.0640313
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.411	515	67.5	0.081711	15	2.12336	8.8	6.4	7.411	520.5	14.8	0.080805
12	7.65	552	72.3	0.126555	7	1.63996	6.8	4.9	7.65	554.4	7.3	0.126719

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	65	8.5	0.0995111	4	0.727992	8.7	6.3	4.55	64.6	4.2	0.0988337
2	5.2	155	20.3	0.077471	6	0.885296	10.6	7.7	5.2	154.9	6.3	0.0794008
3	5.85	270	35.4	0.0680995	6	0.630952	7.5	5.5	5.85	269.4	5.5	0.0646719
4	6.55	407	53.4	0.133091	7	1.46407	17.5	12.7	6.55	405.9	6.7	0.122763
5	6.85	438	57.5	0.0411756	6	0.396234	4.7	3.4	6.85	438.1	5.8	0.0385173
6	7.35	506	66.4	0.0664669	4	0.462413	5.5	4	7.35	506	4	0.0655307
7	8.15	633	83.1	0.0916917	9	1.38711	16.6	12	8.15	632.5	9.1	0.0862137
8	8.45	693	90.9	0.107953	8	1.55681	18.6	13.5	8.45	693.6	8.3	0.10641
9	8.65	745	97.8	0.109046	5	0.8531	10.2	7.4	8.65	745	4.6	0.10423

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 72

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.300 5.200 8.700 5.850 9.400 6.550 17.900 6.850 4.400 7.350 8.400 8.150 15.500 8.450 18.600 8.650 9.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.291 10.200 4.507 10.800 4.600 3.900 4.737 6.400 5.924 17.800 5.996 17.900 6.128 2.200 6.164 3.700 6.210 5.100 6.328 5.100 6.466 2.500 6.512 4.400 7.366 2.500 7.599 7.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 7.800 5.200 10.000 5.850 8.400 6.550 17.600 6.850 4.500 7.350 7.600 8.150 16.300 8.450 18.300 8.650 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.259 11.100 4.482 4.000 4.550 7.900 4.692 6.400 5.200 0.900 5.850 0.900 5.921 17.000 5.977 17.500 6.119 2.000 6.164 2.800 6.205 4.500 6.332 4.900 6.464 2.700 6.504 4.700 6.850 1.300 7.350 1.900 7.614 5.900 8.450 2.000 8.650 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.249 8.200 4.452 6.900 4.550 7.100 4.683 11.300 5.929 16.900 6.000 13.400 6.148 6.600 6.209 5.100 6.341 3.700 6.499 5.200 7.411 8.800 7.650 6.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 12 Return to Lane Navigator
4.550 8.700 5.200 10.600 5.850 7.500 6.550 17.500 6.850 4.700 7.350 5.500 8.150 16.600 8.450 18.600 8.650 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Brown Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Brown Rockfish ---- Go to Display Gel Lane Navigator.