RFE Fish Species Identification Isoelectric Focusing Tools - Blackback Flounder PAGPlate IEF Gel 02

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Blackback Flounder ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes americanus
FDA Seafood List Common Name: Blackback Flounder
FDA Seafood List Market Name: Flounder (Sole)
Unique IEF Gel Code: 19980827gel01
Unique Web Code: bbg02Srr
Tissue Aliquot Code: pleuamer H94-003 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	39	1.7	0.465036	17	12.8122	8.2	6.3	3.5	42.1	16.6	0.434337
2	4.55	352	15.8	0.484009	9	6.83052	4.4	3.4	4.55	351.4	8.6	0.447775
3	5.2	515	23.1	1.02088	6	10.3961	6.7	5.1	5.2	515.3	6	0.975207
4	5.85	809	36.2	2.27636	6	23.0529	14.8	11.4	5.85	810.2	5.7	2.28904
5	6.55	1157	51.8	1.99228	4	13.1577	8.4	6.5	6.55	1157.6	4	1.87051
6	6.85	1246	55.8	1.96144	5	16.4726	10.6	8.2	6.85	1246.6	4.9	1.91599
7	7.35	1429	64	2.31741	9	37.3306	24	18.5	7.35	1429.7	8.6	2.45595
8	8.15	1737	77.8	0.157233	14	3.93202	2.5	1.9	8.15	1737.2	14.1	0.157065
9	8.45	1863	83.4	0.28944	11	5.58755	3.6	2.8	8.45	1863	11.2	0.280904
10	8.65	1938	86.8	0.304963	10	5.12989	3.3	2.5	8.65	1937.3	9.9	0.291288
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2174	97.4	2.12795	6	21.061	13.5	10.4	9.3	2172.9	5.9	2.02731

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.042	196	8.8	2.37571	13	61.3361	16.1	15.6	4.042	205.2	12.6	2.7416
2	4.187	238	10.7	0.0946783	2	0.201803	0.1	0.1	4.187	238.9	1.9	0.0598773
3	4.325	278	12.5	0.650309	6	6.75751	1.8	1.7	4.325	278.1	6	0.639436
4	4.533	338	15.2	0.170933	5	1.31912	0.3	0.3	4.533	337	4.6	0.161502
5	4.628	363	16.3	2.18322	9	38.4191	10.1	9.8	4.628	366.8	9	2.40373
6	4.718	386	17.3	0.332875	5	2.77285	0.7	0.7	4.718	387.3	4.7	0.336413
7	4.793	405	18.2	0.0277957	4	0.195925	0.1	0	4.793	402	3.7	0.0296858
8	4.863	423	19	0.0301724	5	0.263102	0.1	0.1	4.863	421.5	4.8	0.0312058
9	4.926	439	19.7	0.0256283	8	0.39593	0.1	0.1	4.926	443.7	8.4	0.0264492
10	5.012	461	20.7	0.272392	8	3.63074	1	0.9	5.012	461	7.8	0.263937
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.118	488	21.9	0.101016	14	2.48377	0.7	0.6	5.118	491.7	13.6	0.103021
12	5.268	541	24.2	0.0357134	5	0.339083	0.1	0.1	5.268	541.4	5.2	0.0369331
13	5.319	565	25.3	0.0904944	5	0.85138	0.2	0.2	5.319	564.4	5.1	0.0932922
14	5.359	584	26.2	2.27888	5	21.9402	5.8	5.6	5.359	584.2	5.4	2.29426
15	5.395	601	26.9	0.193022	10	4.18188	1.1	1.1	5.395	596.4	10	0.236318
16	5.488	645	28.9	0.0593868	9	0.979315	0.3	0.2	5.488	644.8	9	0.061731
17	5.547	673	30.2	2.23154	4	16.6584	4.4	4.2	5.547	674	4.2	2.25694
18	5.585	691	31	0.0366278	2	0.147313	0	0	5.585	692.6	2.5	0.0336763
19	5.619	707	31.7	2.14917	10	41.1525	10.8	10.5	5.619	713	9.8	2.36502
20	5.674	733	32.9	2.12422	12	44.6714	11.7	11.3	5.674	736.8	11.9	2.11531
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.717	753	33.8	0.872716	13	7.29265	1.9	1.9	5.717	753.2	13.3	0.30849
22	5.772	779	34.9	0.0917704	3	0.282201	0.1	0.1	5.772	780.9	3.2	0.0504204
23	5.864	823	36.9	0.150809	6	1.42898	0.4	0.4	5.864	822.2	6.1	0.132238
24	5.951	866	38.8	0.0789842	5	0.750512	0.2	0.2	5.951	865.9	5.5	0.0776629
25	6.014	897	40.2	0.0919985	2	0.271677	0.1	0.1	6.014	897.2	2.4	0.0651782
26	6.103	941	42.2	0.670624	7	7.34203	1.9	1.9	6.103	939.7	7	0.591021
27	6.133	956	42.9	2.1216	5	13.6459	3.6	3.5	6.133	957.4	4.6	1.6709
28	6.21	994	44.6	1.9356	18	75.2565	19.8	19.1	6.21	980.1	18	2.36448
29	6.275	1026	46	0.150554	8	1.34843	0.4	0.3	6.275	1032.6	8	0.0946154
30	6.356	1066	47.8	0.326916	11	6.11988	1.6	1.6	6.356	1069.9	10.6	0.326469
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	6.394	1085	48.6	1.85555	4	12.3671	3.2	3.1	6.394	1085.7	4.1	1.69931
32	7.081	1335	59.8	0.0309487	8	0.408159	0.1	0.1	7.081	1336.7	7.6	0.0303338
33	7.172	1368	61.3	0.0489819	24	1.59056	0.4	0.4	7.172	1379.1	24	0.0373916
34	7.327	1424	63.8	0.153256	11	2.87189	0.8	0.7	7.327	1423.4	10.5	0.153644
35	8.401	1848	82.8	0.0338424	15	1.01385	0.3	0.3	8.401	1852.8	14.8	0.0386177

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	1.8	0.435023	17	12.0709	8.4	6.7	3.5	41.7	16.6	0.409181
2	4.55	335	15	0.427014	10	7.10831	5	4	4.55	336.8	9.8	0.40886
3	5.2	504	22.6	0.561527	9	8.84637	6.2	4.9	5.2	506	9.1	0.546404
4	5.85	824	37	2.37227	6	27.1464	19	15.2	5.85	822	5.9	2.58651
5	6.55	1168	52.4	0.772586	8	10.7862	7.5	6	6.55	1166	8.3	0.731128
6	6.85	1258	56.4	1.78681	6	16.6939	11.7	9.3	6.85	1258.3	5.7	1.6415
7	7.35	1437	64.4	2.25377	9	37.6079	26.3	21	7.35	1439.4	9.2	2.3023
8	8.15	1752	78.6	0.135122	17	3.98787	2.8	2.2	8.15	1751.8	16.9	0.132882
9	8.45	1875	84.1	0.286406	10	5.17839	3.6	2.9	8.45	1875.4	10.4	0.279711
10	8.65	1951	87.5	0.235332	9	3.81426	2.7	2.1	8.65	1950.9	9.4	0.230023
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2188	98.1	0.794783	8	9.66615	6.8	5.4	9.3	2187.6	8.1	0.670602

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	2.1	0.38914	16	10.4661	2.1	1.9	3.5	49.5	15.8	0.374697
2	4	200	9	2.36928	12	57.8326	11.3	10.5	4	208.7	12.3	2.65721
3	4.144	244	10.9	0.069506	4	0.391303	0.1	0.1	4.144	242.3	4	0.0557956
4	4.275	284	12.7	0.623796	6	6.77778	1.3	1.2	4.275	283.6	6.4	0.600005
5	4.406	324	14.5	0.00840262	5	0.0719912	0	0	4.406	323.3	5.2	0.00778011
6	4.475	345	15.5	0.162138	13	3.53034	0.7	0.6	4.475	350.8	12.9	0.154067
7	4.55	368	16.5	2.19587	12	44.7363	8.8	8.1	4.55	371.2	11.9	2.12691
8	4.655	394	17.7	0.302707	5	1.98584	0.4	0.4	4.655	395.4	4.5	0.246363
9	4.796	429	19.2	0.0573721	5	0.528705	0.1	0.1	4.796	428.8	5.2	0.0568419
10	4.95	467	21	0.242864	9	3.98206	0.8	0.7	4.95	466.7	9.4	0.2392
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.055	493	22.1	0.0610819	18	2.6344	0.5	0.5	5.055	509.1	17.9	0.0832079
12	5.2	529	23.7	0.754434	9	10.8186	2.1	2	5.2	526.9	8.6	0.707804
13	5.282	567	25.4	0.0669473	18	1.04619	0.2	0.2	5.282	572.5	18	0.0328747
14	5.327	588	26.4	1.93105	8	25.9602	5.1	4.7	5.327	590.3	7.9	1.85111
15	5.524	680	30.5	1.55746	7	16.6575	3.3	3	5.524	679.5	6.5	1.43533
16	5.595	713	32	2.13709	12	43.7844	8.6	7.9	5.595	717.7	11.7	2.10905
17	5.65	739	33.2	2.01166	12	32.0035	6.3	5.8	5.65	737.5	11.5	1.56712
18	5.676	751	33.7	1.6456	9	18.6844	3.7	3.4	5.676	750.5	8.8	1.19225
19	5.753	787	35.3	0.0639532	8	0.862932	0.2	0.2	5.753	788.7	7.6	0.0641062
20	5.85	832	37.3	1.76783	7	22.6357	4.4	4.1	5.85	830.8	7.5	1.71278
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.941	873	39.2	0.0447221	7	0.602557	0.1	0.1	5.941	874.3	7.5	0.0455072
22	6.104	947	42.5	1.50603	10	25.9718	5.1	4.7	6.104	944.3	9.7	1.50376
23	6.159	972	43.6	1.69357	18	49.6473	9.7	9	6.159	972.2	17.7	1.58388
24	6.221	1000	44.9	1.96611	12	39.4818	7.7	7.1	6.221	998.3	11.6	1.92816
25	6.303	1037	46.5	0.105763	9	1.58275	0.3	0.3	6.303	1040.4	8.5	0.104742
26	6.391	1077	48.3	0.180893	10	3.52027	0.7	0.6	6.391	1081.6	10.4	0.191111
27	6.437	1098	49.3	1.43334	5	12.6269	2.5	2.3	6.437	1098.2	4.8	1.49346
28	6.55	1149	51.5	0.129485	15	3.60239	0.7	0.7	6.55	1143.6	15.3	0.132784
29	6.85	1247	55.9	0.452343	9	6.7862	1.3	1.2	6.85	1246.5	8.9	0.4306
30	7.129	1349	60.5	0.0396002	10	0.684917	0.1	0.1	7.129	1352.7	9.7	0.0400391
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.35	1430	64.2	2.25685	7	29.1443	5.7	5.3	7.35	1427.4	6.9	2.3684
32	8.15	1733	77.7	0.0718148	16	1.95906	0.4	0.4	8.15	1732.2	16.4	0.0674596
33	8.45	1872	84	0.2192	12	4.49448	0.9	0.8	8.45	1872.6	11.8	0.214318
34	8.65	1950	87.5	0.194101	10	3.42846	0.7	0.6	8.65	1950	10.3	0.187396
35	9.3	2193	98.4	2.0688	6	20.8695	4.1	3.8	9.3	2191.8	6.1	1.92507

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.981	187	8.4	2.37753	12	57.1437	15.9	15.2	3.981	195.4	11.9	2.71247
2	4.124	229	10.3	0.0685658	7	0.770516	0.2	0.2	4.124	228.8	6.6	0.0659803
3	4.267	271	12.2	0.677421	6	6.78695	1.9	1.8	4.267	270.9	5.8	0.662741
4	4.472	331	14.9	0.168586	7	2.01914	0.6	0.5	4.472	331.6	6.9	0.164116
5	4.554	355	15.9	2.23998	9	38.7241	10.7	10.3	4.554	360.1	8.9	2.46621
6	4.654	380	17.1	0.380057	4	3.00053	0.8	0.8	4.654	380.4	4.4	0.381143
7	4.785	413	18.5	0.0452863	6	0.482758	0.1	0.1	4.785	414	5.7	0.0480643
8	4.953	455	20.4	0.198503	7	2.64757	0.7	0.7	4.953	454.1	7.5	0.199966
9	5.04	477	21.4	0.0662002	7	0.752694	0.2	0.2	5.04	476.4	6.5	0.0649581
10	5.092	490	22	0.0575647	6	0.527813	0.1	0.1	5.092	489.4	6	0.0499283
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.14	502	22.5	0.0324575	7	0.45762	0.1	0.1	5.14	499.6	7	0.0368406
12	5.236	534	24	0.0409339	5	0.329036	0.1	0.1	5.236	534.4	4.6	0.0402288
13	5.287	558	25	0.0806049	5	0.810211	0.2	0.2	5.287	559.2	5.4	0.0841591
14	5.327	577	25.9	2.29077	5	21.7418	6	5.8	5.327	577.9	5.4	2.27807
15	5.351	588	26.4	0.475595	6	4.29819	1.2	1.1	5.351	588.3	6.4	0.376814
16	5.467	643	28.9	0.0345019	13	0.835874	0.2	0.2	5.467	637.9	13.4	0.0351229
17	5.518	667	29.9	2.24096	4	15.76	4.4	4.2	5.518	667.8	4.1	2.16492
18	5.561	687	30.8	0.0437329	4	0.254946	0.1	0.1	5.561	686	3.7	0.0385039
19	5.618	714	32	2.15919	8	34.5392	9.6	9.2	5.618	708.1	7.9	2.45156
20	5.66	734	32.9	2.16045	9	39.498	11	10.5	5.66	730.2	9	2.47195
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.69	748	33.6	0.738738	4	5.12623	1.4	1.4	5.69	748.7	4	0.724893
22	5.745	774	34.7	0.121673	6	1.30504	0.4	0.3	5.745	773.7	6.4	0.114245
23	5.847	822	36.9	0.120879	4	0.832006	0.2	0.2	5.847	822.1	4.2	0.111814
24	5.941	866	38.9	0.075388	4	0.571864	0.2	0.2	5.941	865.5	4.3	0.0750988
25	6.048	916	41.1	0.0423856	3	0.198236	0.1	0.1	6.048	916.1	3.1	0.0358407
26	6.097	939	42.1	2.02774	9	32.443	9	8.6	6.097	937.3	8.6	2.12673
27	6.131	955	42.9	2.14225	5	21.6647	6	5.8	6.131	957.9	5.5	2.22944
28	6.189	982	44.1	2.08367	11	42.48	11.8	11.3	6.189	985.4	11.3	2.12377
29	6.24	1006	45.2	0.089886	1	0.0418467	0	0	6.24	1006.6	0.6	0.0372202
30	6.276	1023	45.9	0.122479	9	1.7459	0.5	0.5	6.276	1027.1	8.9	0.110423
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	6.364	1064	47.8	0.192192	16	4.88117	1.4	1.3	6.364	1071.9	16.3	0.168714
32	6.415	1088	48.8	1.86938	3	8.98041	2.5	2.4	6.415	1087.9	3.2	1.58966
33	7.097	1336	60	0.0550394	5	0.51852	0.1	0.1	7.097	1335.8	5.4	0.0543181
34	7.167	1362	61.1	0.0291342	10	0.559302	0.2	0.1	7.167	1363.3	10.5	0.0300587
35	7.27	1400	62.8	0.0748631	18	2.19063	0.6	0.6	7.27	1397.4	17.7	0.0696739
36	7.343	1427	64	0.0906562	8	1.21642	0.3	0.3	7.343	1426.6	7.7	0.0887187
37	8.395	1850	83	0.140566	17	4.12933	1.1	1.1	8.395	1849.5	17.3	0.134281

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	50	2.2	0.420861	19	13.2401	9.9	7.4	3.5	51.9	18.6	0.401394
2	4.55	345	15.5	0.50447	7	6.07209	4.5	3.4	4.55	345.2	7.3	0.467665
3	5.2	510	22.8	0.771152	7	8.54985	6.4	4.8	5.2	509.2	6.6	0.736083
4	5.85	820	36.7	2.38415	5	24.3589	18.2	13.6	5.85	818.3	5.2	2.63216
5	6.55	1158	51.9	0.746204	8	10.09	7.5	5.7	6.55	1156.5	8.2	0.696621
6	6.85	1248	55.9	1.4278	6	12.5115	9.3	7	6.85	1247	5.6	1.26584
7	7.35	1434	64.2	2.32164	8	32.7156	24.4	18.3	7.35	1434	7.6	2.43943
8	8.15	1756	78.7	0.129447	15	3.3851	2.5	1.9	8.15	1755.9	14.9	0.127854
9	8.45	1882	84.3	0.321991	10	5.46556	4.1	3.1	8.45	1881.9	9.9	0.312578
10	8.65	1960	87.8	0.264822	10	4.54828	3.4	2.5	8.65	1958.6	10.1	0.253156
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2196	98.4	1.53599	5	13.0579	9.7	7.3	9.3	2195.5	5.3	1.39993

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 140

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 8.200 4.550 4.400 5.200 6.700 5.850 14.800 6.550 8.400 6.850 10.600 7.350 24.000 8.150 2.500 8.450 3.600 8.650 3.300 9.300 13.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 11 Return to Lane Navigator
4.042 16.100 4.187 0.100 4.325 1.800 4.533 0.300 4.628 10.100 4.718 0.700 4.793 0.100 4.863 0.100 4.926 0.100 5.012 1.000 5.118 0.700 5.268 0.100 5.319 0.200 5.359 5.800 5.395 1.100 5.488 0.300 5.547 4.400 5.585 0 5.619 10.800 5.674 11.700 5.717 1.900 5.772 0.100 5.864 0.400 5.951 0.200 6.014 0.100 6.103 1.900 6.133 3.600 6.210 19.800 6.275 0.400 6.356 1.600 6.394 3.200 7.081 0.100 7.172 0.400 7.327 0.800 8.401 0.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 35 Return to Lane Navigator
3.500 8.400 4.550 5.000 5.200 6.200 5.850 19.000 6.550 7.500 6.850 11.700 7.350 26.300 8.150 2.800 8.450 3.600 8.650 2.700 9.300 6.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 11 Return to Lane Navigator
3.500 2.100 4.000 11.300 4.144 0.100 4.275 1.300 4.406 0 4.475 0.700 4.550 8.800 4.655 0.400 4.796 0.100 4.950 0.800 5.055 0.500 5.200 2.100 5.282 0.200 5.327 5.100 5.524 3.300 5.595 8.600 5.650 6.300 5.676 3.700 5.753 0.200 5.850 4.400 5.941 0.100 6.104 5.100 6.159 9.700 6.221 7.700 6.303 0.300 6.391 0.700 6.437 2.500 6.550 0.700 6.850 1.300 7.129 0.100 7.350 5.700 8.150 0.400 8.450 0.900 8.650 0.700 9.300 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 35 Return to Lane Navigator
3.981 15.900 4.124 0.200 4.267 1.900 4.472 0.600 4.554 10.700 4.654 0.800 4.785 0.100 4.953 0.700 5.040 0.200 5.092 0.100 5.140 0.100 5.236 0.100 5.287 0.200 5.327 6.000 5.351 1.200 5.467 0.200 5.518 4.400 5.561 0.100 5.618 9.600 5.660 11.000 5.690 1.400 5.745 0.400 5.847 0.200 5.941 0.200 6.048 0.100 6.097 9.000 6.131 6.000 6.189 11.800 6.240 0 6.276 0.500 6.364 1.400 6.415 2.500 7.097 0.100 7.167 0.200 7.270 0.600 7.343 0.300 8.395 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 37 Return to Lane Navigator
3.500 9.900 4.550 4.500 5.200 6.400 5.850 18.200 6.550 7.500 6.850 9.300 7.350 24.400 8.150 2.500 8.450 4.100 8.650 3.400 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Blackback Flounder ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Blackback Flounder ---- Go to Display Gel Lane Navigator.