RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Samon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Salmo salar
FDA Seafood List Common Name: Atlantic Samon
FDA Seafood List Market Name: Salmon, Atlantic
Unique IEF Gel Code: 19960314gel01
Unique Web Code: atg01Srr
Tissue Aliquot Code: salmsala I94-002 #09m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	33	2.9	0.234835	7	2.68725	5.5	3.9	3.5	35.6	6.5	0.232416
2	4.55	180	15.9	0.547776	4	3.75813	7.8	5.5	4.55	180.3	4.3	0.49793
3	5.2	265	23.4	0.511552	4	3.32374	6.9	4.9	5.2	264.5	3.8	0.493223
4	5.85	420	37	0.935247	4	6.17295	12.7	9	5.85	420.2	3.6	0.962122
5	6.55	608	53.6	0.537899	4	3.95898	8.2	5.8	6.55	607	4.3	0.517853
6	6.85	652	57.5	0.282874	8	3.46118	7.1	5.1	6.85	652	7.8	0.249452
7	7.35	748	66	0.733136	9	10.4804	21.6	15.4	7.35	745.9	8.7	0.680207
8	8.15	908	80.1	0.128193	8	1.91949	4	2.8	8.15	907	8.5	0.127545
9	8.45	962	84.8	0.260923	7	3.11498	6.4	4.6	8.45	961.7	7.1	0.248435
10	8.65	996	87.8	0.302668	6	3.03669	6.3	4.5	8.65	995.9	6	0.286325
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1112	98.1	0.510408	8	6.55356	13.5	9.6	9.3	1109.7	8.1	0.454388

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.625	189	16.7	0.151969	9	2.27553	2.8	2.3	4.625	188.7	9.2	0.140218
2	5.068	248	21.9	0.146234	8	2.04162	2.5	2	5.068	248.2	8.5	0.135641
3	5.196	265	23.4	0.165746	7	1.86643	2.3	1.9	5.196	266.9	7	0.151036
4	5.316	293	25.9	0.0913196	7	1.24715	1.5	1.2	5.316	295.5	7.5	0.0939448
5	5.485	333	29.4	0.402282	4	2.7177	3.4	2.7	5.485	332.8	4.1	0.375673
6	5.523	342	30.2	0.446727	6	4.34946	5.4	4.3	5.523	342.3	5.7	0.433248
7	5.603	361	31.9	0.196777	5	1.80775	2.2	1.8	5.603	360.6	5.5	0.186027
8	5.641	370	32.7	0.197858	3	1.16491	1.4	1.2	5.641	370.2	3.4	0.192026
9	5.904	434	38.4	0.669606	4	5.09844	6.3	5.1	5.904	434.6	4.4	0.653276
10	6.005	461	40.8	0.415751	3	1.85567	2.3	1.8	6.005	460.6	2.5	0.411511
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.035	469	41.5	0.862205	4	5.51501	6.8	5.5	6.035	469	3.7	0.843961
12	6.121	492	43.5	0.797825	3	4.78858	5.9	4.8	6.121	491.5	3.5	0.777842
13	6.304	541	47.8	0.250877	2	1.01662	1.3	1	6.304	540.8	2.3	0.247844
14	6.453	581	51.4	0.211665	2	0.786637	1	0.8	6.453	581.3	2.1	0.216132
15	6.543	605	53.5	0.43488	11	7.70382	9.6	7.7	6.543	606.9	10.6	0.409883
16	6.606	615	54.4	0.411611	4	1.22601	1.5	1.2	6.606	616.3	3.7	0.188602
17	6.676	625	55.3	0.759107	5	6.89306	8.5	6.9	6.676	626.2	5.3	0.729095
18	7.004	679	60	0.36858	3	1.92471	2.4	1.9	7.004	679.4	3.1	0.353434
19	7.121	701	62	0.242199	9	3.73249	4.6	3.7	7.121	699.4	8.5	0.246782
20	7.222	720	63.7	0.56003	2	2.16828	2.7	2.2	7.222	719.5	2.1	0.572153
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.291	733	64.8	0.122782	2	0.52278	0.6	0.5	7.291	733.2	2.4	0.124153
22	7.385	751	66.4	0.771225	6	7.23145	9	7.2	7.385	748.9	6	0.676519
23	7.556	785	69.4	0.636769	4	5.35375	6.6	5.3	7.556	784.2	4.4	0.686852
24	7.626	799	70.6	0.129858	3	0.623019	0.8	0.6	7.626	799.2	2.8	0.1278
25	7.681	810	71.6	0.0266575	4	0.414239	0.5	0.4	7.681	806.4	4.2	0.0561013
26	8.313	934	82.6	0.112063	8	1.61895	2	1.6	8.313	934.4	8.3	0.110278
27	8.641	993	87.8	0.505616	5	4.68569	5.8	4.7	8.641	993.4	5.2	0.510528

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	33	2.9	0.307244	9	4.71274	9.6	6.5	3.5	34.3	8.9	0.299206
2	4.55	180	15.9	0.484432	4	3.49169	7.1	4.9	4.55	179.8	4.5	0.442377
3	5.2	268	23.7	0.457781	4	3.04176	6.2	4.2	5.2	267.8	3.9	0.438791
4	5.85	421	37.2	0.882251	4	5.98277	12.1	8.3	5.85	421.5	3.7	0.917835
5	6.55	608	53.7	0.523277	4	3.42607	6.9	4.8	6.55	607.5	3.8	0.509025
6	6.85	650	57.4	0.254595	9	3.27545	6.6	4.5	6.85	649.8	8.5	0.217103
7	7.35	742	65.5	0.654723	8	9.20152	18.7	12.8	7.35	740.6	8.4	0.614549
8	8.15	901	79.6	0.174961	12	3.5893	7.3	5	8.15	900.2	12.4	0.163038
9	8.45	959	84.7	0.254463	6	2.69303	5.5	3.7	8.45	958.8	6.1	0.249861
10	8.65	995	87.9	0.269184	5	2.52234	5.1	3.5	8.65	994.1	5.4	0.261692
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1111	98.1	0.565564	8	7.39543	15	10.3	9.3	1109.1	8.5	0.49318

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.233245	8	2.97686	3.4	2.8	3.5	35.6	7.7	0.219457
2	4.55	176	15.6	0.407888	6	4.11736	4.7	3.9	4.55	177.2	6.2	0.375941
3	5.001	235	20.8	0.0934686	10	1.49373	1.7	1.4	5.001	239.2	10.4	0.0811616
4	5.154	255	22.5	0.235194	3	1.07092	1.2	1	5.154	254.5	3	0.201097
5	5.2	261	23.1	0.322286	4	2.10887	2.4	2	5.2	261.6	3.8	0.313689
6	5.287	282	24.9	0.115522	9	1.65471	1.9	1.6	5.287	286.2	8.8	0.105783
7	5.467	325	28.7	0.512326	4	3.27785	3.7	3.1	5.467	324.7	3.6	0.517107
8	5.504	334	29.5	0.4703	5	3.81268	4.3	3.6	5.504	334.5	4.8	0.448395
9	5.588	354	31.3	0.181397	5	1.38716	1.6	1.3	5.588	353.9	4.7	0.165703
10	5.629	364	32.2	0.14877	3	0.917091	1	0.9	5.629	364.1	3.4	0.150954
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.792	403	35.6	0.0901046	4	0.674259	0.8	0.6	5.792	402.4	4.5	0.0853459
12	5.85	417	36.9	0.612571	3	3.60195	4.1	3.4	5.85	417.1	3.2	0.628242
13	5.893	428	37.8	0.63624	4	4.7482	5.4	4.5	5.893	428.7	4.1	0.648139
14	6.005	457	40.4	0.282165	2	0.878657	1	0.8	6.005	456.2	2	0.249674
15	6.032	464	41	0.77501	3	4.52677	5.1	4.3	6.032	464.9	3.3	0.764698
16	6.125	488	43.1	0.718401	3	4.42252	5	4.2	6.125	488.1	3.5	0.719466
17	6.314	537	47.5	0.207271	3	1.08813	1.2	1	6.314	537.2	3	0.206856
18	6.48	580	51.3	0.171804	2	0.73787	0.8	0.7	6.48	580.3	2.3	0.179284
19	6.55	598	52.9	0.143878	3	0.732758	0.8	0.7	6.55	597.6	2.8	0.150279
20	6.676	616	54.5	0.0788928	5	0.505851	0.6	0.5	6.676	613.6	4.7	0.0607042
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.759	628	55.5	0.416432	4	3.15232	3.6	3	6.759	628.3	4.1	0.435062
22	6.85	641	56.7	0.0908245	2	0.350657	0.4	0.3	6.85	641.4	2.1	0.0940244
23	7.029	681	60.2	0.248759	3	1.10341	1.2	1	7.029	680.6	2.8	0.221276
24	7.1	697	61.6	0.178368	11	3.24013	3.7	3.1	7.1	702	10.9	0.168353
25	7.212	722	63.8	0.461749	3	2.31517	2.6	2.2	7.212	721.7	3	0.43673
26	7.265	734	64.9	0.519411	5	4.41895	5	4.2	7.265	732.8	4.8	0.519656
27	7.35	753	66.6	0.681563	5	5.60502	6.3	5.3	7.35	751.8	5.1	0.615593
28	7.553	788	69.7	0.634619	4	4.61578	5.2	4.4	7.553	786.7	4	0.643061
29	7.634	802	70.9	0.137777	3	0.751341	0.8	0.7	7.634	802	3	0.141151
30	7.692	812	71.8	0.0427974	3	0.323467	0.4	0.3	7.692	810.2	3.4	0.054339
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	891	78.8	0.0954294	9	1.47807	1.7	1.4	8.15	889.4	8.7	0.095728
32	8.388	941	83.2	0.187632	9	2.67669	3	2.5	8.388	937.3	8.9	0.168765
33	8.45	954	84.4	0.186333	10	2.97813	3.4	2.8	8.45	954.8	9.7	0.173323
34	8.65	991	87.6	0.462855	5	4.08896	4.6	3.9	8.65	991.1	5	0.463449
35	9.3	1107	97.9	0.667682	6	6.61769	7.5	6.3	9.3	1105.7	6.1	0.613196

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.615	185	16.3	0.149447	9	2.20345	2.5	1.9	4.615	183.5	8.8	0.140889
2	5.02	238	21	0.152742	11	2.7441	3.2	2.3	5.02	237.5	10.9	0.142534
3	5.166	257	22.7	0.211862	9	2.69502	3.1	2.3	5.166	258.7	8.6	0.175854
4	5.298	285	25.2	0.130208	14	2.82893	3.3	2.4	5.298	285	13.8	0.115838
5	5.464	325	28.7	0.487986	4	3.07527	3.5	2.6	5.464	325.3	3.5	0.492889
6	5.505	335	29.6	0.451385	5	3.58349	4.1	3	5.505	335.2	4.6	0.437862
7	5.584	354	31.3	0.197625	5	1.65768	1.9	1.4	5.584	354.3	5.2	0.180791
8	5.626	364	32.2	0.195381	3	1.17092	1.4	1	5.626	364.2	3.4	0.194145
9	5.784	402	35.5	0.155064	5	1.24378	1.4	1	5.784	401.7	5.2	0.135499
10	5.881	426	37.6	0.795037	3	4.74353	5.5	4	5.881	426.1	3.5	0.770587
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.99	454	40.1	0.387443	2	1.47125	1.7	1.2	5.99	453.3	2.1	0.40406
12	6.014	460	40.6	0.910058	4	5.53279	6.4	4.7	6.014	461.2	3.5	0.890458
13	6.107	484	42.8	0.814211	3	4.21493	4.9	3.6	6.107	484.2	3	0.805083
14	6.283	529	46.7	0.240433	2	0.934394	1.1	0.8	6.283	528.7	2.2	0.244015
15	6.443	570	50.4	0.203089	2	0.787682	0.9	0.7	6.443	570.4	2.1	0.210743
16	6.54	595	52.6	0.417277	11	7.39325	8.5	6.2	6.54	596.9	10.9	0.383471
17	6.602	605	53.4	0.400248	3	1.06123	1.2	0.9	6.602	605.9	3.4	0.178711
18	6.671	615	54.3	0.771231	5	6.79477	7.8	5.7	6.671	616	5.2	0.733737
19	6.983	669	59.1	0.390785	3	1.82187	2.1	1.5	6.983	669.3	2.7	0.386726
20	7.088	691	61	0.240533	8	3.55422	4.1	3	7.088	689.5	8.2	0.245496
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.179	710	62.7	0.57259	2	2.27476	2.6	1.9	7.179	709.7	2.3	0.569812
22	7.24	723	63.9	0.106767	2	0.469341	0.5	0.4	7.24	722.8	2.5	0.10617
23	7.326	741	65.5	0.694444	5	6.02705	7	5.1	7.326	738.6	4.7	0.722787
24	7.494	773	68.3	0.583894	4	4.05729	4.7	3.4	7.494	773.9	3.7	0.612699
25	7.579	789	69.7	0.10328	3	0.584218	0.7	0.5	7.579	789.2	3.2	0.103857
26	7.627	798	70.5	0.0461257	3	0.248057	0.3	0.2	7.627	798.4	3	0.047433
27	8.328	931	82.2	0.231293	18	7.5763	8.7	6.4	8.328	949.6	18	0.237475
28	8.628	987	87.2	0.658619	6	5.91688	6.8	5	8.628	988.1	6.1	0.547146

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.353178	10	5.86053	11.6	7.8	3.5	36.5	10.2	0.322714
2	4.55	176	15.5	0.494711	4	3.64324	7.2	4.8	4.55	175.8	4.5	0.457012
3	5.2	261	23.1	0.43722	4	3.13789	6.2	4.2	5.2	260.7	4.1	0.429089
4	5.85	418	36.9	0.864926	4	6.01293	11.9	8	5.85	418.7	3.8	0.884858
5	6.55	596	52.7	0.544127	4	3.87959	7.7	5.1	6.55	595.6	4.2	0.519749
6	6.85	640	56.5	0.354101	9	4.64513	9.2	6.2	6.85	640.4	8.7	0.301887
7	7.35	738	65.2	0.734711	9	10.7212	21.2	14.2	7.35	735.3	9.1	0.663468
8	8.15	900	79.5	0.125515	8	1.68298	3.3	2.2	8.15	899.4	7.6	0.124736
9	8.45	955	84.4	0.198654	5	1.84258	3.6	2.4	8.45	955	5.2	0.201698
10	8.65	990	87.5	0.283368	5	2.3273	4.6	3.1	8.65	990	4.7	0.279635
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1106	97.7	0.671602	6	6.86992	13.6	9.1	9.3	1103.9	6.2	0.621949

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 123

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 5.500 4.550 7.800 5.200 6.900 5.850 12.700 6.550 8.200 6.850 7.100 7.350 21.600 8.150 4.000 8.450 6.400 8.650 6.300 9.300 13.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 11 Return to Lane Navigator
4.625 2.800 5.068 2.500 5.196 2.300 5.316 1.500 5.485 3.400 5.523 5.400 5.603 2.200 5.641 1.400 5.904 6.300 6.005 2.300 6.035 6.800 6.121 5.900 6.304 1.300 6.453 1.000 6.543 9.600 6.606 1.500 6.676 8.500 7.004 2.400 7.121 4.600 7.222 2.700 7.291 0.600 7.385 9.000 7.556 6.600 7.626 0.800 7.681 0.500 8.313 2.000 8.641 5.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 13 PeakCount: 27 Return to Lane Navigator
3.500 9.600 4.550 7.100 5.200 6.200 5.850 12.100 6.550 6.900 6.850 6.600 7.350 18.700 8.150 7.300 8.450 5.500 8.650 5.100 9.300 15.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 14 PeakCount: 11 Return to Lane Navigator
3.500 3.400 4.550 4.700 5.001 1.700 5.154 1.200 5.200 2.400 5.287 1.900 5.467 3.700 5.504 4.300 5.588 1.600 5.629 1.000 5.792 0.800 5.850 4.100 5.893 5.400 6.005 1.000 6.032 5.100 6.125 5.000 6.314 1.200 6.480 0.800 6.550 0.800 6.676 0.600 6.759 3.600 6.850 0.400 7.029 1.200 7.100 3.700 7.212 2.600 7.265 5.000 7.350 6.300 7.553 5.200 7.634 0.800 7.692 0.400 8.150 1.700 8.388 3.000 8.450 3.400 8.650 4.600 9.300 7.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 15 PeakCount: 35 Return to Lane Navigator
4.615 2.500 5.020 3.200 5.166 3.100 5.298 3.300 5.464 3.500 5.505 4.100 5.584 1.900 5.626 1.400 5.784 1.400 5.881 5.500 5.990 1.700 6.014 6.400 6.107 4.900 6.283 1.100 6.443 0.900 6.540 8.500 6.602 1.200 6.671 7.800 6.983 2.100 7.088 4.100 7.179 2.600 7.240 0.500 7.326 7.000 7.494 4.700 7.579 0.700 7.627 0.300 8.328 8.700 8.628 6.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 16 PeakCount: 28 Return to Lane Navigator
3.500 11.600 4.550 7.200 5.200 6.200 5.850 11.900 6.550 7.700 6.850 9.200 7.350 21.200 8.150 3.300 8.450 3.600 8.650 4.600 9.300 13.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Samon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Samon ---- Go to Display Gel Lane Navigator.