RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Schoolmaster ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus apodus
FDA Seafood List Common Name: Schoolmaster
FDA Seafood List Market Name: Schoolmaster
Unique IEF Gel Code: 19970212gel08
Unique Web Code: ash03Srr
Tissue Aliquot Code: lutjapod H94-003 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	114	13.2	0.0642181	5	0.49261	5.4	4.1	4.55	114.3	4.5	0.0616724
2	5.2	206	23.8	0.0790701	5	0.691149	7.5	5.8	5.2	206.1	5.1	0.0758619
3	5.85	320	37	0.109572	5	0.977371	10.7	8.2	5.85	319.8	5.2	0.106875
4	6.55	459	53.1	0.125654	8	1.65449	18	13.9	6.55	458.2	7.9	0.117892
5	6.85	491	56.8	0.0385492	5	0.335356	3.7	2.8	6.85	491	5.1	0.0372204
6	7.35	555	64.2	0.0708617	5	0.607408	6.6	5.1	7.35	554.6	5.1	0.0678155
7	8.15	677	78.4	0.0953491	8	1.27095	13.9	10.7	8.15	676.5	7.9	0.0912336
8	8.45	742	85.9	0.138156	6	1.50372	16.4	12.6	8.45	740.9	6.4	0.13247
9	8.65	794	91.9	0.10943	4	0.83398	9.1	7	8.65	793.9	4.4	0.106214
10	9.3	848	98.1	0.0375548	13	0.806112	8.8	6.8	9.3	844	12.7	0.0357343

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.907	22	2.5	0.313194	11	5.66475	15	11.8	3.907	24.4	11	0.29117
2	4.159	57	6.6	0.057608	6	0.587102	1.6	1.2	4.159	57.5	6	0.0548054
3	4.345	83	9.6	0.432249	6	4.4459	11.8	9.3	4.345	83.4	5.7	0.440055
4	5.29	218	25.2	0.0604656	6	0.611687	1.6	1.3	5.29	217	5.7	0.060398
5	5.38	234	27.1	0.418203	5	3.42318	9.1	7.2	5.38	234.1	4.6	0.415822
6	5.51	257	29.7	0.0837444	10	1.21936	3.2	2.6	5.51	255.7	9.6	0.0716324
7	5.6	273	31.6	0.0431233	3	0.249287	0.7	0.5	5.6	273.1	3.5	0.0406619
8	5.65	282	32.6	0.0691905	5	0.613178	1.6	1.3	5.65	282.4	4.9	0.0708711
9	5.774	304	35.2	0.0620803	5	0.485701	1.3	1	5.774	303.2	4.6	0.0600709
10	5.888	325	37.6	0.540604	4	3.46883	9.2	7.3	5.888	325.7	3.6	0.544889
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.973	342	39.6	0.245901	5	1.88442	5	3.9	5.973	341.6	4.6	0.231358
12	6.039	355	41.1	0.120746	4	0.927678	2.5	1.9	6.039	356	4.1	0.128687
13	6.074	362	41.9	0.149551	3	0.731632	1.9	1.5	6.074	362.7	3	0.139922
14	6.14	375	43.4	0.0663987	3	0.349314	0.9	0.7	6.14	374.7	2.9	0.0680199
15	6.245	396	45.8	0.0524556	6	0.479448	1.3	1	6.245	394.4	6	0.044853
16	6.316	410	47.5	0.258572	3	1.33947	3.6	2.8	6.316	409.8	2.9	0.260138
17	6.396	426	49.3	0.364587	3	1.76698	4.7	3.7	6.396	426	2.8	0.357315
18	6.477	442	51.2	0.248179	2	1.05528	2.8	2.2	6.477	441.6	2.3	0.258297
19	6.522	451	52.2	0.100295	3	0.58449	1.6	1.2	6.522	452	3.2	0.10453
20	6.65	468	54.2	0.059139	4	0.342322	0.9	0.7	6.65	468.5	3.6	0.053007
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.711	475	55	0.191596	2	0.673208	1.8	1.4	6.711	475.2	1.9	0.197795
22	6.789	484	56	0.37334	2	1.5037	4	3.1	6.789	483.8	2.2	0.378261
23	7.039	516	59.7	0.330033	5	2.84136	7.5	5.9	7.039	518.2	5.1	0.315216
24	7.437	570	66	0.10837	14	2.39843	6.4	5	7.437	568.9	14	0.0966386

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	111	12.9	0.027695	7	0.33434	4.3	3.3	4.55	109.4	6.6	0.0284273
2	5.2	200	23.2	0.0368241	11	0.715447	9.2	7.1	5.2	203.7	11.4	0.0353859
3	5.85	317	36.8	0.0835799	6	0.864635	11.1	8.6	5.85	317.2	6	0.0818857
4	6.55	456	52.9	0.0928364	8	1.27184	16.3	12.6	6.55	455	8.4	0.0858961
5	6.85	493	57.2	0.0232522	7	0.24127	3.1	2.4	6.85	492.1	6.5	0.0208797
6	7.35	561	65.1	0.0431125	5	0.370244	4.7	3.7	7.35	561.5	5.1	0.0407263
7	8.15	679	78.8	0.0884769	7	1.07735	13.8	10.7	8.15	678.2	7.2	0.0840516
8	8.45	743	86.2	0.116523	7	1.34732	17.3	13.4	8.45	742.8	6.7	0.113984
9	8.65	795	92.2	0.0886207	5	0.716052	9.2	7.1	8.65	794.2	4.7	0.0858412
10	9.3	843	97.8	0.033571	13	0.864812	11.1	8.6	9.3	836.9	13	0.0374589

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.957	27	3.1	0.191656	10	3.17673	11	9.3	3.957	28	9.6	0.185967
2	4.176	58	6.7	0.0247436	6	0.259606	0.9	0.8	4.176	56.9	6.2	0.0237229
3	4.331	80	9.2	0.33112	5	3.2418	11.2	9.5	4.331	80.5	5.5	0.333788
4	4.55	111	12.8	0.0553958	4	0.407383	1.4	1.2	4.55	110.8	4.3	0.0529951
5	5.2	203	23.5	0.0554606	4	0.370019	1.3	1.1	5.2	202.7	4	0.0527151
6	5.25	212	24.5	0.0393643	5	0.370661	1.3	1.1	5.25	212.3	5.5	0.0382897
7	5.333	227	26.2	0.35731	5	2.86662	9.9	8.4	5.333	226.7	4.6	0.353405
8	5.449	248	28.7	0.0333445	13	0.699943	2.4	2	5.449	243	12.6	0.0313121
9	5.549	266	30.8	0.0111186	3	0.040494	0.1	0.1	5.549	269.6	2.9	0.0077771
10	5.604	276	31.9	0.0255936	5	0.209788	0.7	0.6	5.604	276.7	5	0.0236115
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.737	300	34.7	0.0185726	5	0.177494	0.6	0.5	5.737	301	5.4	0.0186727
12	5.793	310	35.8	0.0182683	2	0.0460496	0.2	0.1	5.793	310.1	1.6	0.0163413
13	5.889	328	37.9	0.40126	4	3.00555	10.4	8.8	5.889	328.3	4.3	0.391822
14	5.981	346	40	0.185771	6	1.74484	6	5.1	5.981	345.8	5.7	0.173255
15	6.057	361	41.7	0.115082	4	0.875158	3	2.6	6.057	360.8	4.4	0.111433
16	6.087	367	42.4	0.105991	3	0.461355	1.6	1.3	6.087	367.4	2.8	0.0914232
17	6.148	379	43.8	0.0355131	3	0.16686	0.6	0.5	6.148	379.1	2.7	0.0352601
18	6.245	398	46	0.0292405	6	0.269127	0.9	0.8	6.245	396.2	6.2	0.0243442
19	6.301	409	47.3	0.184632	3	0.970863	3.4	2.8	6.301	408.8	3	0.182339
20	6.372	423	48.9	0.261278	3	1.29142	4.5	3.8	6.372	423	2.8	0.258766
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.443	437	50.5	0.144938	3	0.693053	2.4	2	6.443	436.9	2.7	0.145003
22	6.499	448	51.8	0.07648	6	0.775813	2.7	2.3	6.499	448.3	6.1	0.0717028
23	6.674	472	54.6	0.105712	2	0.398029	1.4	1.2	6.674	472.1	2.1	0.108144
24	6.762	482	55.7	0.229844	2	0.894122	3.1	2.6	6.762	481.8	2.2	0.225351
25	6.85	492	56.9	0.0304303	3	0.176648	0.6	0.5	6.85	493	3.3	0.0306264
26	7.043	519	60	0.209299	5	1.65377	5.7	4.8	7.043	520.7	5.2	0.178512
27	7.35	562	65	0.0543674	6	0.447752	1.6	1.3	7.35	562	5.5	0.045671
28	7.459	578	66.8	0.0597419	10	1.0127	3.5	3	7.459	576.5	10.1	0.056704
29	8.15	679	78.5	0.0391352	6	0.453253	1.6	1.3	8.15	679.7	6.3	0.0403043
30	8.45	745	86.1	0.0744864	6	0.783682	2.7	2.3	8.45	744.9	6	0.073998
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	795	91.9	0.060833	5	0.47016	1.6	1.4	8.65	795.1	4.5	0.0588741
32	9.3	845	97.7	0.0251071	10	0.459546	1.6	1.3	9.3	841.2	9.5	0.0271762

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.921	23	2.7	0.349961	11	6.22963	15.6	11.3	3.921	25.8	10.8	0.323986
2	4.167	58	6.7	0.0597564	7	0.697335	1.7	1.3	4.167	57.1	7.1	0.0550624
3	4.343	83	9.6	0.449159	6	4.87883	12.2	8.8	4.343	82.6	6	0.457439
4	5.26	216	25	0.0629581	5	0.552091	1.4	1	5.26	214.8	5	0.0627803
5	5.353	233	26.9	0.40444	5	3.78529	9.5	6.8	5.353	232.7	5.3	0.406182
6	5.49	258	29.8	0.0842206	11	1.411	3.5	2.5	5.49	256.6	11.5	0.0694425
7	5.583	275	31.8	0.0595739	4	0.356055	0.9	0.6	5.583	275.8	3.7	0.0543475
8	5.638	285	32.9	0.0814816	5	0.743342	1.9	1.3	5.638	285.7	5	0.083193
9	5.764	308	35.6	0.0699933	6	0.661657	1.7	1.2	5.764	308.3	5.5	0.067502
10	5.888	331	38.3	0.533818	4	3.60814	9	6.5	5.888	331.1	3.9	0.521568
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.975	348	40.2	0.246744	5	1.97187	4.9	3.6	5.975	347.5	4.7	0.236786
12	6.042	361	41.7	0.125602	4	0.942077	2.4	1.7	6.042	361.6	4.1	0.128549
13	6.078	368	42.5	0.131812	3	0.608286	1.5	1.1	6.078	368.3	2.9	0.119653
14	6.134	379	43.8	0.0790827	3	0.4619	1.2	0.8	6.134	379.6	3.2	0.0814424
15	6.237	399	46.1	0.0517325	5	0.402822	1	0.7	6.237	397.2	5.3	0.0426826
16	6.298	411	47.5	0.273193	3	1.37692	3.4	2.5	6.298	410.6	2.8	0.274456
17	6.375	426	49.2	0.37957	3	1.8834	4.7	3.4	6.375	425.6	2.8	0.384013
18	6.452	441	51	0.235641	3	1.06593	2.7	1.9	6.452	441.3	2.5	0.240141
19	6.509	452	52.3	0.10201	4	0.692547	1.7	1.3	6.509	452.6	3.8	0.103688
20	6.653	471	54.5	0.0586392	6	0.565194	1.4	1	6.653	471.4	5.7	0.0554784
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.728	479	55.4	0.202073	2	0.69501	1.7	1.3	6.728	479	2.1	0.190576
22	6.812	488	56.4	0.364063	2	1.44969	3.6	2.6	6.812	488.3	2.3	0.362187
23	6.935	504	58.3	0.027398	6	0.252392	0.6	0.5	6.935	501.5	5.5	0.0257615
24	7.049	520	60.1	0.315727	5	2.75086	6.9	5	7.049	521.4	5	0.31098
25	7.415	572	66.1	0.0888491	12	1.94745	4.9	3.5	7.415	572	12.1	0.090589

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	115	13.2	0.0533605	5	0.435636	5.7	4.4	4.55	115.5	4.9	0.0498869
2	5.2	208	24	0.07179	5	0.552198	7.3	5.6	5.2	208.1	4.5	0.0690521
3	5.85	328	37.8	0.108182	5	0.863216	11.4	8.8	5.85	328.3	4.5	0.107139
4	6.55	463	53.3	0.100476	9	1.38834	18.3	14.1	6.55	461.9	8.5	0.0917378
5	6.85	493	56.8	0.0320608	6	0.341687	4.5	3.5	6.85	492.9	6.1	0.0313858
6	7.35	564	65	0.0376802	5	0.336317	4.4	3.4	7.35	562.5	5.1	0.036915
7	8.15	681	78.5	0.0935052	7	1.12922	14.9	11.5	8.15	681	7.2	0.0889069
8	8.45	748	86.2	0.112723	7	1.27549	16.8	13	8.45	747.1	6.6	0.109369
9	8.65	799	92.1	0.0832757	4	0.642413	8.5	6.5	8.65	798.9	4.5	0.081045
10	9.3	849	97.8	0.034853	11	0.637746	8.4	6.5	9.3	844.6	10.6	0.0338761

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 111

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.400 5.200 7.500 5.850 10.700 6.550 18.000 6.850 3.700 7.350 6.600 8.150 13.900 8.450 16.400 8.650 9.100 9.300 8.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.907 15.000 4.159 1.600 4.345 11.800 5.290 1.600 5.380 9.100 5.510 3.200 5.600 0.700 5.650 1.600 5.774 1.300 5.888 9.200 5.973 5.000 6.039 2.500 6.074 1.900 6.140 0.900 6.245 1.300 6.316 3.600 6.396 4.700 6.477 2.800 6.522 1.600 6.650 0.900 6.711 1.800 6.789 4.000 7.039 7.500 7.437 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 24 Return to Lane Navigator
4.550 4.300 5.200 9.200 5.850 11.100 6.550 16.300 6.850 3.100 7.350 4.700 8.150 13.800 8.450 17.300 8.650 9.200 9.300 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.957 11.000 4.176 0.900 4.331 11.200 4.550 1.400 5.200 1.300 5.250 1.300 5.333 9.900 5.449 2.400 5.549 0.100 5.604 0.700 5.737 0.600 5.793 0.200 5.889 10.400 5.981 6.000 6.057 3.000 6.087 1.600 6.148 0.600 6.245 0.900 6.301 3.400 6.372 4.500 6.443 2.400 6.499 2.700 6.674 1.400 6.762 3.100 6.850 0.600 7.043 5.700 7.350 1.600 7.459 3.500 8.150 1.600 8.450 2.700 8.650 1.600 9.300 1.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 32 Return to Lane Navigator
3.921 15.600 4.167 1.700 4.343 12.200 5.260 1.400 5.353 9.500 5.490 3.500 5.583 0.900 5.638 1.900 5.764 1.700 5.888 9.000 5.975 4.900 6.042 2.400 6.078 1.500 6.134 1.200 6.237 1.000 6.298 3.400 6.375 4.700 6.452 2.700 6.509 1.700 6.653 1.400 6.728 1.700 6.812 3.600 6.935 0.600 7.049 6.900 7.415 4.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 25 Return to Lane Navigator
4.550 5.700 5.200 7.300 5.850 11.400 6.550 18.300 6.850 4.500 7.350 4.400 8.150 14.900 8.450 16.800 8.650 8.500 9.300 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Schoolmaster ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Schoolmaster ---- Go to Display Gel Lane Navigator.