Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Schoolmaster  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus apodus
FDA Seafood List Common Name: Schoolmaster
FDA Seafood List Market Name: Schoolmaster
Unique IEF Gel Code: 19940825gel05
Unique Web Code: ash02Srr
Tissue Aliquot Code: lutjapod H94-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5511813.80.06063460.56081710.584.55118.25.60.0567588
25.222326.10.080230740.5544210.47.95.2223.44.10.0761197
35.8533238.90.10239140.66901112.59.55.85331.83.70.100781
46.5545953.70.086856360.94217517.613.46.55458.96.40.082625
57.35555650.023644960.2462814.63.57.35555.56.30.0221986
68.1567779.30.040030790.63125111.898.15676.390.0396954
78.4573886.40.062680970.72099813.510.38.45738.46.60.0617918
88.6578792.20.039818950.3416446.44.98.65785.95.10.037569
99.383097.20.065805960.67241212.69.69.3829.76.40.0593187
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.916222.60.35949963.2667411.89.43.91621.65.50.334705
24.008364.20.14881282.440528.87.14.00832.88.20.168133
34.1656070.046480360.477771.71.44.16559.76.20.0433272
44.3619010.50.4909754.678651713.54.36190.35.30.494288
55.30323627.60.031883650.2404220.90.75.303235.34.50.0300087
65.39825229.50.45811343.3972512.39.85.398251.34.30.444934
75.528274320.035096550.291671.10.85.528273.85.30.0311347
85.628291340.0119021150.2440740.90.75.628286.914.70.0093817
95.68230035.10.027939430.1117370.40.35.682300.72.70.0229368
105.832037.40.033139980.4124791.51.25.8321.57.90.029591
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.91834139.90.4662732.508359.17.25.918341.23.10.462924
12635641.60.24061531.396615.146356.13.30.240705
136.07136943.20.085151740.5864032.11.76.071368.83.90.0844198
146.10337543.90.073898620.2955951.10.96.103374.52.40.0697447
156.158385450.052790330.26512210.86.158384.82.80.0538957
166.24540146.90.028234330.1413930.50.46.245401.12.70.0293655
176.33241748.80.37592331.998297.25.86.332416.730.380733
186.40843150.40.017502350.1426760.50.46.408431.14.60.0174138
196.528453530.065941640.4678291.71.46.528453.540.0653832
206.657470550.068722440.4059631.51.26.657470.73.50.0647432
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.731479560.10892440.7330022.72.16.731477.93.80.107884
226.797487570.16117420.6157582.21.86.797486.82.20.161165
237.07852160.90.19545541.589135.84.67.078521.64.50.200347
247.363556650.0553076100.8940963.22.67.363556.59.50.0528689
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55119140.046232850.3535436.754.55118.94.50.0439535
25.2213250.082238540.59696711.38.45.2212.74.30.0792455
35.8532438.10.089850940.64895812.39.15.85324.44.10.0887161
46.5545453.30.085701660.94773917.913.36.55453.56.40.0829635
57.3555264.90.024345870.2969425.64.27.35551.87.20.0231168
68.1567479.20.049367680.70221513.39.88.15673.48.20.0482636
78.4573686.50.052666880.70694513.49.98.45736.17.60.0522274
88.6578091.70.040810350.3474836.64.98.65779.650.0388401
99.382697.10.062994470.69285213.19.79.3825.57.20.0546533
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.886303.50.21676351.814578.173.886295.40.190664
23.961414.80.10039681.482386.65.73.96138.57.80.10694
34.137677.90.020084650.1751330.80.74.13767.15.10.0193586
44.3339611.30.39511853.6633316.414.14.33395.75.20.400828
54.5512815.10.045874250.3701471.71.44.55128.64.80.0434058
65.222426.40.030299140.1947020.90.85.2224.13.50.0312499
75.27423627.80.011395130.05107410.20.25.274236.12.60.0109382
85.36625129.50.34403542.5267111.39.75.366250.34.20.34288
95.495272320.021775350.1589870.70.65.495272.14.60.0194419
105.58828733.80.0168939100.2799361.31.15.588287.9100.0157753
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.64329634.80.017324340.07347050.30.35.643298.33.60.0116309
125.77831837.40.015750450.1170150.50.55.778318.34.80.0136332
135.91134140.10.37073542.3196210.48.95.911340.63.70.355302
145.99335641.90.16849741.152175.24.45.993355.940.161041
156.06336943.40.069332440.4919912.21.96.063368.94.10.0681328
166.10137644.20.044963420.2074780.90.86.101375.12.40.0488597
176.15638645.40.034545830.1821340.80.76.156385.62.90.0358586
186.24340247.30.016845830.1035250.50.46.243400.73.50.0169021
196.31841648.90.25514931.405846.35.46.318415.93.10.253611
206.443150.70.017353230.089310.40.36.4431.52.80.0181021
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.51945353.30.059409760.619322.82.46.519452.25.90.0588586
226.68447555.90.083908760.7443633.32.96.684474.25.60.0744463
236.74248256.70.10264120.44204821.76.742483.12.20.11449
246.81649157.80.017627550.1496220.70.66.816490.44.80.017733
257.02951760.80.1335451.12895.14.47.029518.44.90.131095
267.3555665.40.065230980.8544963.83.37.35555.97.80.0615559
278.1568680.70.018857110.3398391.51.38.15683.711.50.016701
288.4574087.10.041420660.4102281.81.68.45740.35.80.0397291
298.6577991.60.026022360.22123810.98.657785.60.0222326
309.382697.20.043787780.5734342.62.29.3826.28.30.0389057
PeakCounter: 30
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.874242.80.32396462.9853110.88.93.874235.50.304008
23.958374.30.14222282.198247.96.63.95834.28.10.152393
34.113617.20.042910460.4150451.51.24.11361.35.80.0401816
44.3079110.70.44886364.5419616.413.64.30790.55.60.455093
55.26624028.20.022007330.1326630.50.45.266238.43.40.0218445
65.3525429.80.45366743.4937712.610.55.35253.34.40.448214
75.4727432.20.04134770.4851151.81.55.47273.97.30.0377052
85.56629034.10.008014320.03674360.10.15.566290.42.50.0083521
95.63230135.40.024634930.1310770.50.45.632300.52.90.025076
105.74632037.60.035922180.463571.71.45.746321.18.50.0308932
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.87634240.20.44833932.4950597.55.876341.93.10.448593
125.96335842.10.2247231.3860154.15.963358.43.40.226698
136.0437243.70.084470540.5867722.11.86.04371.63.90.084255
146.06737744.30.079787830.3298451.216.067377.22.70.0697069
156.12238745.50.05394230.28698210.96.122387.42.90.0551907
166.20940347.40.029689230.1874760.70.66.209402.83.40.0309887
176.29141849.10.40570332.046967.46.16.291417.82.80.408205
186.37343350.90.012847550.1106950.40.36.373431.94.70.013324
196.48845453.30.063749150.55310721.76.488453.55.20.060023
206.62247455.70.1186891.812856.65.46.622474.18.80.116641
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.714485570.12660530.4968681.81.56.714484.630.093164
227.01352161.20.1727951.570195.74.77.013521.15.40.16459
237.353562660.042727110.7628632.82.37.353562.8110.0391135
249.35883297.80.00957490.1493310.50.49.358828.89.30.0090173
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5512514.80.096730660.88213311.99.24.55124.85.60.0893136
25.223027.20.10297660.99950513.510.45.2229.75.80.097675
35.8534140.30.12411740.9318412.59.75.85340.54.40.120603
46.5546855.30.11117171.2737517.113.36.55468.26.80.106234
57.3556366.50.02698150.2536413.42.77.35563.45.40.026614
68.1568180.40.0364487110.668281978.15679.910.60.0356763
78.4573686.90.079233270.9656931310.18.45735.96.90.0786681
88.6578392.40.046750250.4269195.74.58.65782.15.40.0448251
99.382697.50.092698371.0279313.810.79.3825.67.20.0808879
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 105
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 30 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln