RFE Fish Species Identification Isoelectric Focusing Tools - Arrowtooth Flounder Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Atheresthes stomias
FDA Seafood List Common Name: Arrowtooth Flounder
FDA Seafood List Market Name: Flounder, Arrowtooth
Unique IEF Gel Code: 19930813gel02
Unique Web Code: arh01Srr
Tissue Aliquot Code: athestom 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	95	11.2	0.0734226	7	0.793803	8.6	6.9	4.55	95.7	6.7	0.0667678
2	5.2	193	22.7	0.082249	5	0.690291	7.5	6	5.2	193.3	4.9	0.0795568
3	5.85	296	34.9	0.11767	5	1.03398	11.2	9	5.85	296.4	5	0.116156
4	6.55	431	50.8	0.129661	7	1.44644	15.6	12.6	6.55	429.5	6.7	0.122512
5	6.85	459	54.1	0.0183824	6	0.172879	1.9	1.5	6.85	459.4	5.7	0.0170453
6	7.35	521	61.4	0.0470335	5	0.409186	4.4	3.6	7.35	520.9	5	0.046639
7	8.15	644	75.9	0.102835	7	1.24189	13.4	10.8	8.15	643.4	7	0.100547
8	8.45	712	83.9	0.0762058	7	0.90498	9.8	7.9	8.45	712.3	6.6	0.0768919
9	8.65	762	89.8	0.0549794	5	0.46701	5	4.1	8.65	762.1	5	0.0530546
10	9.3	823	96.9	0.147861	8	2.09197	22.6	18.3	9.3	821.2	8.3	0.141822

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.16	36	4.2	0.170234	10	3.00469	11.4	9.2	4.16	36.9	9.9	0.171889
2	4.433	78	9.2	0.00842366	1	0	0	0	4.433	69	1	0
3	4.569	99	11.6	0.14714	15	3.84139	14.6	11.7	4.569	102.9	14.7	0.147423
4	4.83	139	16.4	0.0210644	5	0.1889	0.7	0.6	4.83	138.6	5.1	0.0210159
5	5.2	196	23.1	0.0499263	3	0.314613	1.2	1	5.2	195.2	3.4	0.0529388
6	5.28	209	24.6	0.423294	5	3.5752	13.5	10.9	5.28	209.6	4.8	0.423286
7	5.398	228	26.8	0.00598153	3	0.0305915	0.1	0.1	5.398	228.8	3.1	0.00560565
8	5.565	255	30	0.0173156	3	0.0970483	0.4	0.3	5.565	254.6	3.3	0.0165443
9	5.689	275	32.4	0.54322	4	4.24004	16.1	12.9	5.689	275.6	4.4	0.538419
10	5.763	287	33.8	0.182532	6	1.92887	7.3	5.9	5.763	286.8	6.2	0.174339
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	301	35.4	0.0689028	8	0.959643	3.6	2.9	5.85	300.6	8.2	0.0661798
12	6.018	332	39.1	0.233747	3	1.21466	4.6	3.7	6.018	332.1	2.9	0.234374
13	6.078	343	40.4	0.0229052	6	0.204609	0.8	0.6	6.078	342.9	5.6	0.0207617
14	6.176	361	42.5	0.106344	5	0.960939	3.6	2.9	6.176	361.5	5.5	0.0988621
15	6.268	378	44.5	0.1113	4	0.705301	2.7	2.2	6.268	377.5	4.2	0.0936596
16	6.333	390	45.9	0.150923	7	1.84886	7	5.6	6.333	389.5	6.8	0.152385
17	6.425	407	47.9	0.0205267	4	0.111395	0.4	0.3	6.425	409.3	4	0.0156059
18	6.55	430	50.6	0.0421854	8	0.604537	2.3	1.8	6.55	430.5	8.1	0.0423699
19	6.762	451	53.1	0.0610612	3	0.321656	1.2	1	6.762	451.6	3.1	0.0591146
20	7.35	525	61.8	0.0311688	9	0.472254	1.8	1.4	7.35	524.5	9.3	0.0286853
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	720	84.7	0.0458441	8	0.595146	2.3	1.8	8.45	719.7	7.9	0.0422611
22	8.65	764	89.9	0.0265319	4	0.204758	0.8	0.6	8.65	764.6	4.4	0.0262506
23	9.3	822	96.7	0.0795178	7	0.964005	3.7	2.9	9.3	820.9	7.3	0.0746584

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.174	38	4.5	0.172959	10	3.23284	13.3	8.9	4.174	38.1	10.5	0.173759
2	4.438	78	9.2	0.0158299	1	0	0	0	4.438	68	1	0
3	4.629	107	12.7	0.155751	16	4.41632	18.1	12.1	4.629	106.5	15.6	0.159229
4	4.854	141	16.7	0.0264528	5	0.239033	1	0.7	4.854	140.4	5.3	0.0256439
5	5.228	198	23.5	0.0178177	2	0.0717157	0.3	0.2	5.228	196.3	2.4	0.0169364
6	5.302	210	24.9	0.463916	5	3.78936	15.6	10.4	5.302	210.8	4.7	0.453411
7	5.425	230	27.3	0.00949183	4	0.073191	0.3	0.2	5.425	230.5	4.4	0.00946297
8	5.579	255	30.2	0.0188096	4	0.114593	0.5	0.3	5.579	255.5	3.5	0.018218
9	5.702	275	32.6	0.574378	4	4.091	16.8	11.2	5.702	275.8	3.9	0.586584
10	5.782	288	34.1	0.206509	7	2.50816	10.3	6.9	5.782	288.5	7.4	0.191012
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.022	331	39.2	0.247291	3	1.25664	5.2	3.5	6.022	331.1	2.8	0.249561
12	6.081	342	40.5	0.0312019	5	0.268173	1.1	0.7	6.081	341.7	5.1	0.0298004
13	6.166	358	42.4	0.108566	5	0.966115	4	2.7	6.166	359.7	5.2	0.104097
14	6.258	375	44.4	0.0994508	2	0.36844	1.5	1	6.258	375	2.4	0.0874606
15	6.327	388	46	0.163873	8	2.22419	9.1	6.1	6.327	387.7	7.8	0.160161
16	6.424	406	48.1	0.0318308	5	0.337047	1.4	0.9	6.424	404.3	5.2	0.0365457
17	6.52	424	50.2	0.00807707	3	0.0365285	0.2	0.1	6.52	424.9	2.7	0.00750663
18	6.732	447	53	0.0693226	3	0.355085	1.5	1	6.732	447.2	2.9	0.0696746

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	94	11.1	0.0668946	5	0.561056	6.5	5.3	4.55	93.8	4.9	0.0645715
2	5.2	191	22.6	0.139562	4	0.989331	11.4	9.3	5.2	190.9	4.1	0.137282
3	5.85	297	35.1	0.0993351	5	0.90308	10.4	8.5	5.85	296.4	5.2	0.0984873
4	6.55	429	50.7	0.133907	7	1.45883	16.8	13.7	6.55	427.9	6.7	0.122949
5	6.85	457	54	0.0149208	4	0.103937	1.2	1	6.85	457.2	4.3	0.0137465
6	7.35	521	61.6	0.0444295	5	0.374404	4.3	3.5	7.35	521.6	4.8	0.0435746
7	8.15	649	76.7	0.0991843	7	1.2058	13.9	11.3	8.15	648.8	6.8	0.0994909
8	8.45	711	84	0.0861224	6	0.881517	10.2	8.3	8.45	710.7	5.9	0.084722
9	8.65	759	89.7	0.0574852	5	0.524888	6	4.9	8.65	758.5	5.2	0.0568272
10	9.3	821	97	0.13804	7	1.67432	19.3	15.7	9.3	820	7	0.134242

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.183	39	4.6	0.193746	9	3.01778	12.1	9.7	4.183	39.9	9	0.189548
2	4.443	79	9.3	0.00975451	1	0	0	0	4.443	69	1	0
3	4.587	101	11.9	0.156831	14	3.92814	15.7	12.6	4.587	105.4	14	0.158169
4	4.848	141	16.6	0.026061	5	0.225933	0.9	0.7	4.848	140	5.2	0.0247228
5	5.2	195	23	0.0410125	3	0.234228	0.9	0.7	5.2	194.4	3.2	0.0413147
6	5.285	209	24.6	0.42305	5	3.5458	14.2	11.3	5.285	209.7	4.7	0.421441
7	5.407	229	27	0.0140977	4	0.109742	0.4	0.4	5.407	230.3	4.3	0.0142414
8	5.564	255	30.1	0.0158332	4	0.113121	0.5	0.4	5.564	254.7	4.1	0.015561
9	5.686	275	32.4	0.542348	4	3.86771	15.5	12.4	5.686	275.6	4	0.543886
10	5.759	287	33.8	0.206084	7	2.49842	10	8	5.759	286.8	6.9	0.203124
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	302	35.6	0.0633102	6	0.682503	2.7	2.2	5.85	300.4	5.7	0.0673982
12	6.008	330	38.9	0.284935	3	1.43006	5.7	4.6	6.008	330.4	2.9	0.274904
13	6.07	341	40.2	0.0572265	8	0.829169	3.3	2.7	6.07	340.8	7.9	0.0594693
14	6.166	358	42.2	0.107836	4	0.794951	3.2	2.5	6.166	358.6	4.5	0.10023
15	6.256	374	44.1	0.0944713	3	0.388417	1.6	1.2	6.256	374.2	2.5	0.0862413
16	6.324	386	45.5	0.138686	7	1.70929	6.8	5.5	6.324	385.6	7	0.138705
17	6.426	404	47.6	0.0166831	2	0.052022	0.2	0.2	6.426	404.7	2.4	0.0124177
18	6.55	426	50.2	0.0172373	7	0.21578	0.9	0.7	6.55	425.8	6.8	0.0177948
19	6.746	448	52.8	0.0567133	3	0.273315	1.1	0.9	6.746	447.8	2.7	0.0571394
20	8.45	721	85	0.0146411	8	0.194139	0.8	0.6	8.45	722.6	7.7	0.0142465
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	767	90.4	0.00822272	4	0.0806558	0.3	0.3	8.65	765	4.2	0.0108179
22	9.3	824	97.2	0.0539381	9	0.83516	3.3	2.7	9.3	820.9	9.3	0.0507471

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.22	47	5.5	0.218866	9	3.3486	13.5	9.7	4.22	47.7	8.9	0.212452
2	4.472	85	10	0.0221981	1	0	0	0	4.472	72	1	0
3	4.619	107	12.6	0.185522	14	4.58093	18.5	13.2	4.619	111.2	13.7	0.188656
4	4.878	146	17.2	0.0301108	10	0.485027	2	1.4	4.878	142.1	10.2	0.0269311
5	5.24	201	23.7	0.0132641	2	0.0620886	0.3	0.2	5.24	199.5	2.4	0.0147815
6	5.32	214	25.2	0.492224	5	3.94599	15.9	11.4	5.32	215	4.6	0.487493
7	5.442	234	27.6	0.0121764	4	0.0935747	0.4	0.3	5.442	234.1	4.3	0.0121472
8	5.583	257	30.3	0.0213616	4	0.124107	0.5	0.4	5.583	257.5	3.5	0.0197345
9	5.712	278	32.7	0.580956	4	3.94588	15.9	11.4	5.712	278	3.8	0.586611
10	5.779	289	34	0.212562	7	2.57735	10.4	7.4	5.779	289.6	7.4	0.196812
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.015	331	39	0.268945	3	1.27661	5.2	3.7	6.015	331	2.7	0.268336
12	6.08	343	40.4	0.0203805	4	0.129812	0.5	0.4	6.08	342.2	3.7	0.0195312
13	6.161	358	42.2	0.128191	6	1.20534	4.9	3.5	6.161	359.2	5.9	0.115382
14	6.247	374	44.1	0.124934	3	0.60469	2.4	1.7	6.247	374.2	3.1	0.111403
15	6.312	386	45.5	0.150419	7	1.87426	7.6	5.4	6.312	385.8	6.9	0.153159
16	6.415	405	47.7	0.0135105	3	0.0532634	0.2	0.2	6.415	405.9	2.7	0.0110219
17	6.523	425	50.1	0.0157547	6	0.167243	0.7	0.5	6.523	427.8	6	0.0157306
18	6.726	449	52.9	0.0623238	3	0.291977	1.2	0.8	6.726	448.5	2.6	0.0640973

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	98	11.5	0.0779301	5	0.690182	7.2	5.8	4.55	97.8	5.3	0.0731214
2	5.2	194	22.9	0.136422	4	1.03459	10.8	8.7	5.2	194	4.4	0.132582
3	5.85	299	35.2	0.107434	5	0.87483	9.1	7.4	5.85	298.9	4.6	0.10636
4	6.55	434	51.1	0.128977	7	1.49938	15.7	12.6	6.55	432.3	7	0.121066
5	6.85	465	54.8	0.0193714	6	0.227288	2.4	1.9	6.85	463.2	6.2	0.020607
6	7.35	528	62.2	0.0450587	5	0.386704	4	3.3	7.35	528.8	4.7	0.0459716
7	8.15	654	77	0.114172	7	1.32397	13.8	11.1	8.15	653.1	6.6	0.113804
8	8.45	719	84.7	0.0635009	10	1.09474	11.4	9.2	8.45	719.6	9.7	0.0639444
9	8.65	765	90.1	0.0689644	4	0.527524	5.5	4.4	8.65	765	4.3	0.0684923
10	9.3	824	97.1	0.175765	6	1.90486	19.9	16	9.3	823.6	6.2	0.173506

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 111

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.600 5.200 7.500 5.850 11.200 6.550 15.600 6.850 1.900 7.350 4.400 8.150 13.400 8.450 9.800 8.650 5.000 9.300 22.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.160 11.400 4.433 0 4.569 14.600 4.830 0.700 5.200 1.200 5.280 13.500 5.398 0.100 5.565 0.400 5.689 16.100 5.763 7.300 5.850 3.600 6.018 4.600 6.078 0.800 6.176 3.600 6.268 2.700 6.333 7.000 6.425 0.400 6.550 2.300 6.762 1.200 7.350 1.800 8.450 2.300 8.650 0.800 9.300 3.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 23 Return to Lane Navigator
4.174 13.300 4.438 0 4.629 18.100 4.854 1.000 5.228 0.300 5.302 15.600 5.425 0.300 5.579 0.500 5.702 16.800 5.782 10.300 6.022 5.200 6.081 1.100 6.166 4.000 6.258 1.500 6.327 9.100 6.424 1.400 6.520 0.200 6.732 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 6.500 5.200 11.400 5.850 10.400 6.550 16.800 6.850 1.200 7.350 4.300 8.150 13.900 8.450 10.200 8.650 6.000 9.300 19.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.183 12.100 4.443 0 4.587 15.700 4.848 0.900 5.200 0.900 5.285 14.200 5.407 0.400 5.564 0.500 5.686 15.500 5.759 10.000 5.850 2.700 6.008 5.700 6.070 3.300 6.166 3.200 6.256 1.600 6.324 6.800 6.426 0.200 6.550 0.900 6.746 1.100 8.450 0.800 8.650 0.300 9.300 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.220 13.500 4.472 0 4.619 18.500 4.878 2.000 5.240 0.300 5.320 15.900 5.442 0.400 5.583 0.500 5.712 15.900 5.779 10.400 6.015 5.200 6.080 0.500 6.161 4.900 6.247 2.400 6.312 7.600 6.415 0.200 6.523 0.700 6.726 1.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 7.200 5.200 10.800 5.850 9.100 6.550 15.700 6.850 2.400 7.350 4.000 8.150 13.800 8.450 11.400 8.650 5.500 9.300 19.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.