RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19930823gel02
Unique Web Code: acg02Srr
Tissue Aliquot Code: gadumorh 001 #4 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	45	3.8	0.249018	8	3.22847	16.4	12	3.5	46.8	8	0.226621
2	4.55	199	17	0.149369	6	1.48466	7.6	5.5	4.55	198.5	6.1	0.137655
3	5.2	280	24	0.137364	6	1.36513	6.9	5.1	5.2	279.5	5.7	0.136015
4	5.85	421	36	0.208328	6	2.11505	10.8	7.9	5.85	420.4	6	0.200099
5	6.55	605	51.8	0.168173	5	1.53164	7.8	5.7	6.55	605.3	5.2	0.166951
6	6.85	642	54.9	0.0621261	7	0.702043	3.6	2.6	6.85	642.6	6.8	0.0580944
7	7.35	736	63	0.109801	6	1.1983	6.1	4.5	7.35	736	6.2	0.109299
8	8.15	896	76.6	0.0627599	10	1.11627	5.7	4.2	8.15	897.2	10.1	0.0623308
9	8.45	968	82.8	0.108651	6	1.27166	6.5	4.7	8.45	967.9	6.5	0.110729
10	8.65	1005	86	0.108037	5	1.00526	5.1	3.7	8.65	1005.3	5.2	0.108488
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1133	96.9	0.438473	7	4.64517	23.6	17.3	9.3	1131.6	7	0.375468

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	44	3.8	0.2288	8	2.86712	8.2	7.1	3.5	46.1	7.6	0.212089
2	3.859	98	8.4	0.0249166	8	0.367635	1.1	0.9	3.859	99.7	8.1	0.0254536
3	4.165	144	12.3	0.344557	6	3.8226	10.9	9.5	4.165	144.3	6.1	0.353821
4	4.344	171	14.6	0.170089	5	1.61099	4.6	4	4.344	170.8	5.3	0.172532
5	4.55	202	17.3	0.0990457	8	1.26597	3.6	3.1	4.55	202.9	7.5	0.0946989
6	4.788	232	19.8	0.027794	5	0.225789	0.6	0.6	4.788	232.5	4.7	0.027366
7	4.907	247	21.1	0.00618035	2	0.0220636	0.1	0.1	4.907	247.9	2.3	0.00539949
8	5.049	265	22.7	0.0265797	4	0.150824	0.4	0.4	5.049	264.7	3.6	0.0238551
9	5.2	284	24.3	0.0542986	5	0.457336	1.3	1.1	5.2	284.9	5.2	0.0496207
10	5.269	299	25.6	0.243787	6	2.42906	6.9	6	5.269	298.7	5.8	0.236278
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.324	311	26.6	0.0896096	4	0.566317	1.6	1.4	5.324	311.2	3.6	0.0882284
12	5.394	326	27.9	0.0298572	5	0.237987	0.7	0.6	5.394	327.4	4.5	0.0295165
13	5.49	347	29.7	0.0103447	5	0.0858473	0.2	0.2	5.49	348.4	5.2	0.0092954
14	5.606	372	31.8	0.00894686	2	0.0174499	0	0	5.606	370.1	2.2	0.00451489
15	5.666	385	32.9	0.0297653	9	0.482788	1.4	1.2	5.666	383.4	9.2	0.0295415
16	5.772	408	34.9	0.215033	6	2.25531	6.4	5.6	5.772	408.7	5.9	0.216086
17	5.85	425	36.4	0.10988	8	1.09456	3.1	2.7	5.85	423.6	8.1	0.0765278
18	5.88	433	37	0.160094	7	1.87279	5.3	4.6	5.88	434.2	7.4	0.143267
19	6.013	468	40	0.108053	11	1.90898	5.5	4.7	6.013	465.9	11.4	0.0941037
20	6.047	477	40.8	0.0595441	3	0.131648	0.4	0.3	6.047	478.8	2.7	0.0275686
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.153	505	43.2	0.0912231	5	0.723296	2.1	1.8	6.153	504.5	4.6	0.08941
22	6.296	543	46.4	0.053911	6	0.509296	1.5	1.3	6.296	543.3	5.7	0.0503291
23	6.323	550	47	0.0375757	3	0.109997	0.3	0.3	6.323	551.2	2.9	0.0217731
24	6.387	567	48.5	0.0330537	4	0.221956	0.6	0.5	6.387	567.2	4	0.0313132
25	6.444	582	49.8	0.02275	6	0.216828	0.6	0.5	6.444	582.2	5.6	0.0219637
26	6.55	610	52.2	0.0633111	6	0.682162	1.9	1.7	6.55	608.7	6.4	0.0603704
27	6.85	647	55.3	0.0480398	6	0.47833	1.4	1.2	6.85	646.3	5.7	0.0476556
28	7.011	676	57.8	0.0242986	7	0.307358	0.9	0.8	7.011	675.1	7.5	0.0231223
29	7.133	698	59.7	0.0182215	9	0.239163	0.7	0.6	7.133	696.9	9	0.0149868
30	7.222	714	61.1	0.0156967	16	0.468107	1.3	1.2	7.222	728	15.8	0.0167207
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.35	737	63	0.123131	6	1.21692	3.5	3	7.35	736.3	5.8	0.117577
32	7.488	765	65.4	0.0230746	5	0.178967	0.5	0.4	7.488	764.4	4.9	0.0207431
33	8.15	899	76.9	0.0373413	24	1.77106	5.1	4.4	8.15	889.7	24	0.0416348
34	8.45	964	82.5	0.0583304	6	0.642047	1.8	1.6	8.45	963.8	6.2	0.0588065
35	8.65	1004	85.9	0.0996424	6	1.00956	2.9	2.5	8.65	1003.7	5.9	0.096874
36	9.3	1132	96.8	0.349599	8	4.36345	12.5	10.8	9.3	1130.9	7.9	0.310709

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.966	113	9.7	0.0105478	10	0.344823	1.7	1.4	3.966	104.3	9.8	0.0198233
2	4.157	142	12.2	0.336908	6	3.55763	17.4	14	4.157	142.2	5.8	0.343592
3	4.322	167	14.3	0.159413	5	1.56074	7.6	6.1	4.322	167	5.5	0.160366
4	4.577	205	17.6	0.0313163	10	0.50184	2.4	2	4.577	202.5	10.1	0.0280332
5	4.731	225	19.3	0.0271689	5	0.221671	1.1	0.9	4.731	226.2	5.5	0.0228125
6	4.962	255	21.8	0.0221014	5	0.160211	0.8	0.6	4.962	255.5	4.6	0.0197884
7	5.038	265	22.7	0.0188638	2	0.0828738	0.4	0.3	5.038	265.3	2.4	0.019185
8	5.123	276	23.6	0.0039431	3	0.0229332	0.1	0.1	5.123	276.1	2.7	0.00475395
9	5.228	292	25	0.186203	6	1.90984	9.3	7.5	5.228	291.4	6.2	0.174489
10	5.279	303	25.9	0.130211	4	0.790967	3.9	3.1	5.279	302.9	4.1	0.1087
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.364	321	27.5	0.0324595	16	0.894008	4.4	3.5	5.364	315.7	16.4	0.0308155
12	5.476	345	29.5	0.00210765	1	0	0	0	5.476	342	1	0
13	5.541	359	30.7	0.00661617	4	0.0384568	0.2	0.2	5.541	360.1	3.8	0.00574854
14	5.621	376	32.2	0.0136954	7	0.124734	0.6	0.5	5.621	378.1	6.5	0.0108239
15	5.789	412	35.3	0.144765	9	2.49733	12.2	9.8	5.789	411.8	9.4	0.149105
16	5.884	434	37.2	0.115019	9	1.78463	8.7	7	5.884	434.8	8.7	0.115273
17	5.977	459	39.3	0.0414962	2	0.095805	0.5	0.4	5.977	457.7	2.4	0.0223811
18	6.003	466	39.9	0.0769045	7	0.83291	4.1	3.3	6.003	466.4	6.6	0.0714834
19	6.033	474	40.6	0.0642828	4	0.284481	1.4	1.1	6.033	474	3.8	0.0423752
20	6.063	482	41.3	0.0198587	5	0.206981	1	0.8	6.063	480	4.6	0.0256212
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.108	494	42.3	0.00994714	3	0.0725385	0.4	0.3	6.108	495.5	2.7	0.0148901
22	6.142	503	43.1	0.0837934	4	0.577342	2.8	2.3	6.142	503.3	4	0.0804951
23	6.206	520	44.5	0.0061144	11	0.159916	0.8	0.6	6.206	513.5	11.5	0.00785486
24	6.288	542	46.4	0.0415638	5	0.383418	1.9	1.5	6.288	541.6	5.3	0.0408568
25	6.322	551	47.2	0.0366519	4	0.213507	1	0.8	6.322	551.1	3.5	0.0343931
26	6.389	569	48.7	0.0451034	7	0.474405	2.3	1.9	6.389	568.5	6.7	0.0398499
27	6.43	580	49.7	0.0490225	6	0.500155	2.4	2	6.43	580.4	6.1	0.0459734
28	6.584	616	52.7	0.0129911	7	0.131152	0.6	0.5	6.584	619.1	6.5	0.0113106
29	6.98	671	57.4	0.0336697	15	0.867365	4.2	3.4	6.98	668.8	14.9	0.0327932
30	7.116	696	59.6	0.0191819	4	0.104156	0.5	0.4	7.116	695.6	3.6	0.0164276
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.198	711	60.9	0.0369483	5	0.244254	1.2	1	7.198	710.1	5.3	0.0260185
32	7.268	724	62	0.0324025	10	0.581586	2.8	2.3	7.268	721.7	10.2	0.0320947
33	7.345	738	63.2	0.0182937	4	0.106503	0.5	0.4	7.345	739.8	4.4	0.0136649
34	7.436	756	64.7	0.0179466	6	0.170247	0.8	0.7	7.436	756.3	5.7	0.0167712

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.6	0.244331	7	2.83304	13.6	9.6	3.5	43.6	7.1	0.223864
2	4.55	202	17.3	0.1489	6	1.48132	7.1	5	4.55	202.4	6.2	0.134216
3	5.2	289	24.7	0.152652	5	1.3375	6.4	4.5	5.2	288.9	5.1	0.14897
4	5.85	426	36.4	0.182823	8	2.63224	12.7	8.9	5.85	424.3	8.1	0.183504
5	6.55	615	52.6	0.166314	5	1.52273	7.3	5.2	6.55	614.7	5.2	0.165729
6	6.85	648	55.4	0.0716678	7	0.809318	3.9	2.7	6.85	648.7	6.7	0.0678012
7	7.35	742	63.4	0.11604	6	1.19839	5.8	4.1	7.35	741.2	5.8	0.116425
8	8.15	898	76.8	0.0782948	11	1.48109	7.1	5	8.15	896.7	10.8	0.0770311
9	8.45	968	82.7	0.122997	6	1.1684	5.6	4	8.45	968.1	5.7	0.116409
10	8.65	1007	86.1	0.131035	6	1.28701	6.2	4.4	8.65	1007.1	5.7	0.126464
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1134	96.9	0.4095	8	5.02907	24.2	17	9.3	1131.7	7.8	0.363101

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.6	0.219042	8	2.69324	8.3	6.7	3.5	43.6	7.5	0.201383
2	3.876	100	8.5	0.0243538	9	0.377413	1.2	0.9	3.876	97.1	9.3	0.0228138
3	4.148	142	12.1	0.341139	6	3.74641	11.5	9.3	4.148	142.1	6	0.350379
4	4.323	169	14.4	0.140774	5	1.35187	4.1	3.3	4.323	168.7	5.4	0.142059
5	4.55	204	17.4	0.0808824	10	1.34334	4.1	3.3	4.55	202.4	10.1	0.0747409
6	4.772	233	19.9	0.028847	6	0.325106	1	0.8	4.772	234.5	6.2	0.02961
7	4.856	244	20.9	0.0152664	4	0.0711675	0.2	0.2	4.856	243.9	3.6	0.011039
8	5.108	277	23.7	0.00384713	3	0.0268456	0.1	0.1	5.108	277.4	2.8	0.00538238
9	5.2	289	24.7	0.0451445	9	1.22604	3.8	3	5.2	295.5	9.2	0.0754941
10	5.262	302	25.8	0.139199	4	1.18742	3.6	2.9	5.262	304.9	4.1	0.163063
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.319	314	26.8	0.0768796	4	0.512201	1.6	1.3	5.319	314.5	3.8	0.0759279
12	5.395	330	28.2	0.0620229	7	0.789054	2.4	2	5.395	330.4	7	0.0634161
13	5.499	352	30.1	0.0142029	5	0.0971731	0.3	0.2	5.499	352.7	4.9	0.0111776
14	5.655	385	32.9	0.0336706	8	0.475135	1.5	1.2	5.655	385.8	8	0.0334827
15	5.779	411	35.1	0.201343	6	2.05032	6.3	5.1	5.779	411.3	5.8	0.200198
16	5.85	426	36.4	0.103392	6	0.932405	2.9	2.3	5.85	423.5	6.4	0.0816979
17	5.888	436	37.3	0.154616	7	1.99735	6.1	4.9	5.888	435.6	7.5	0.150364
18	5.981	460	39.3	0.0614596	9	0.978988	3	2.4	5.981	461.9	9.4	0.0589964
19	6.015	469	40.1	0.106399	4	0.552317	1.7	1.4	6.015	469.8	3.9	0.0791092
20	6.05	478	40.9	0.0478	4	0.316526	1	0.8	6.05	478.1	3.8	0.0470009
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.154	505	43.2	0.0931408	4	0.647972	2	1.6	6.154	504.2	4.2	0.0869472
22	6.3	543	46.4	0.0569069	9	0.679429	2.1	1.7	6.3	544.2	8.6	0.0446687
23	6.396	568	48.5	0.0486382	6	0.414733	1.3	1	6.396	567.3	5.7	0.0412741
24	6.446	581	49.7	0.0342783	6	0.3345	1	0.8	6.446	580.3	5.5	0.0340232
25	6.55	608	52	0.0791362	5	0.711467	2.2	1.8	6.55	607.6	5.1	0.0780195
26	6.688	625	53.4	0.00906214	3	0.0453322	0.1	0.1	6.688	624.8	2.9	0.00887248
27	6.85	645	55.1	0.0525783	6	0.546542	1.7	1.4	6.85	644.4	6	0.0512544
28	7.027	678	57.9	0.0245271	9	0.367507	1.1	0.9	7.027	677.6	8.7	0.0237123
29	7.113	694	59.3	0.0255326	5	0.210427	0.6	0.5	7.113	693.5	4.8	0.0245742
30	7.151	701	59.9	0.0324158	3	0.168592	0.5	0.4	7.151	701.4	3.1	0.0304702
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.226	715	61.1	0.013003	12	0.445274	1.4	1.1	7.226	725.8	11.6	0.0217032
32	7.35	738	63.1	0.147869	6	1.42278	4.4	3.5	7.35	737.8	5.8	0.138245
33	7.501	767	65.6	0.0455462	6	0.428619	1.3	1.1	7.501	767.2	5.6	0.0428495
34	8.15	892	76.2	0.0516092	18	1.56509	4.8	3.9	8.15	885.9	18	0.0490568
35	8.45	965	82.5	0.0591518	7	0.73816	2.3	1.8	8.45	964.3	7	0.0598754
36	8.65	1007	86.1	0.0805316	5	0.724486	2.2	1.8	8.65	1005.8	5.1	0.0804392
37	9.3	1138	97.3	0.127973	18	2.1006	6.4	5.2	9.3	1138.3	18	0.0658422

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.802	87	7.4	0.0312907	11	0.535311	2.6	2.1	3.802	85.9	10.6	0.028603
2	4.178	143	12.2	0.340363	6	3.83715	18.4	14.9	4.178	142.9	6.2	0.348376
3	4.359	170	14.5	0.168924	5	1.61782	7.8	6.3	4.359	170.3	5.3	0.1734
4	4.624	208	17.8	0.0342878	8	0.454479	2.2	1.8	4.624	208.7	8.2	0.0312171
5	4.805	231	19.7	0.0481797	5	0.403783	1.9	1.6	4.805	231.9	5.5	0.0416705
6	4.883	241	20.6	0.00300075	1	0	0	0	4.883	221	1	0
7	4.945	249	21.3	0.00503692	2	0.0187063	0.1	0.1	4.945	248.6	2.4	0.00431274
8	5.016	258	22.1	0.0106262	6	0.162433	0.8	0.6	5.016	262.7	6.4	0.014229
9	5.102	269	23	0.041202	2	0.125375	0.6	0.5	5.102	268.6	2	0.0355243
10	5.173	278	23.8	0.0326022	2	0.147742	0.7	0.6	5.173	278.2	2.4	0.0341096
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.272	297	25.4	0.27868	5	2.61441	12.5	10.2	5.272	296.1	5.3	0.278791
12	5.328	309	26.4	0.126859	3	0.652636	3.1	2.5	5.328	308.8	2.9	0.128424
13	5.393	323	27.6	0.0533606	5	0.406005	1.9	1.6	5.393	322.8	4.6	0.0499964
14	5.501	346	29.6	0.0121743	7	0.116847	0.6	0.5	5.501	346.2	6.8	0.00971834
15	5.575	362	30.9	0.0146284	2	0.0427493	0.2	0.2	5.575	362.4	2.4	0.0102107
16	5.626	373	31.9	0.025684	10	0.331678	1.6	1.3	5.626	374.1	9.6	0.0194167
17	5.757	401	34.3	0.259661	5	2.06833	9.9	8	5.757	401.5	4.6	0.254246
18	5.873	427	36.5	0.169799	5	1.37626	6.6	5.4	5.873	426.5	4.6	0.167929
19	5.972	453	38.7	0.0690055	5	0.566935	2.7	2.2	5.972	453.2	4.8	0.066093
20	6.003	461	39.4	0.0940337	4	0.520443	2.5	2	6.003	460.6	3.5	0.0830054
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.037	470	40.2	0.06932	3	0.366892	1.8	1.4	6.037	469.7	3.1	0.0673705
22	6.133	495	42.3	0.113936	4	0.699681	3.4	2.7	6.133	494.9	3.7	0.107452
23	6.286	535	45.7	0.0808395	6	0.68996	3.3	2.7	6.286	535.6	5.5	0.0705346
24	6.366	556	47.5	0.0405808	4	0.27045	1.3	1.1	6.366	555.8	4.3	0.0354389
25	6.428	572	48.9	0.0300039	9	0.439141	2.1	1.7	6.428	572.2	9.4	0.026269
26	6.582	608	52	0.0120389	6	0.119245	0.6	0.5	6.582	607.8	6.5	0.0104062
27	6.952	660	56.4	0.0365221	14	0.835906	4	3.3	6.952	656.5	13.8	0.0341038
28	7.096	687	58.7	0.0269581	4	0.193633	0.9	0.8	7.096	685.9	4.4	0.0248953
29	7.171	701	59.9	0.0318723	3	0.112764	0.5	0.4	7.171	700.9	3.5	0.0183011
30	7.23	712	60.9	0.0342338	13	0.787361	3.8	3.1	7.23	714	12.8	0.0347014
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.326	730	62.4	0.0142684	4	0.0569908	0.3	0.2	7.326	730.4	4.4	0.00726355
32	7.445	753	64.4	0.0199694	8	0.273957	1.3	1.1	7.445	751.8	8.1	0.0191628

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.6	0.215356	9	3.23412	15.8	11.8	3.5	43.6	9	0.202704
2	4.55	193	16.5	0.151232	6	1.49813	7.3	5.5	4.55	193	6.1	0.139456
3	5.2	274	23.4	0.146246	6	1.41768	6.9	5.2	5.2	273.9	5.5	0.14418
4	5.85	416	35.5	0.204137	6	2.22438	10.9	8.1	5.85	415.9	6.4	0.196651
5	6.55	600	51.2	0.182025	5	1.67224	8.2	6.1	6.55	599.5	5.2	0.180333
6	6.85	637	54.4	0.0712356	7	0.770534	3.8	2.8	6.85	637.3	6.5	0.0668164
7	7.35	731	62.4	0.13638	6	1.50956	7.4	5.5	7.35	730.9	6.3	0.135586
8	8.15	890	76	0.0731657	10	1.25917	6.2	4.6	8.15	890.3	10.1	0.0703502
9	8.45	963	82.2	0.0971956	6	1.02838	5	3.8	8.45	962.9	5.9	0.0977536
10	8.65	1000	85.4	0.104267	5	0.960551	4.7	3.5	8.65	999.7	5.3	0.102633
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1129	96.4	0.458346	7	4.86871	23.8	17.8	9.3	1127	6.7	0.408089

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 172

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 16.400 4.550 7.600 5.200 6.900 5.850 10.800 6.550 7.800 6.850 3.600 7.350 6.100 8.150 5.700 8.450 6.500 8.650 5.100 9.300 23.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 8.200 3.859 1.100 4.165 10.900 4.344 4.600 4.550 3.600 4.788 0.600 4.907 0.100 5.049 0.400 5.200 1.300 5.269 6.900 5.324 1.600 5.394 0.700 5.490 0.200 5.606 0 5.666 1.400 5.772 6.400 5.850 3.100 5.880 5.300 6.013 5.500 6.047 0.400 6.153 2.100 6.296 1.500 6.323 0.300 6.387 0.600 6.444 0.600 6.550 1.900 6.850 1.400 7.011 0.900 7.133 0.700 7.222 1.300 7.350 3.500 7.488 0.500 8.150 5.100 8.450 1.800 8.650 2.900 9.300 12.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 11 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 36 Return to Lane Navigator
3.966 1.700 4.157 17.400 4.322 7.600 4.577 2.400 4.731 1.100 4.962 0.800 5.038 0.400 5.123 0.100 5.228 9.300 5.279 3.900 5.364 4.400 5.476 0 5.541 0.200 5.621 0.600 5.789 12.200 5.884 8.700 5.977 0.500 6.003 4.100 6.033 1.400 6.063 1.000 6.108 0.400 6.142 2.800 6.206 0.800 6.288 1.900 6.322 1.000 6.389 2.300 6.430 2.400 6.584 0.600 6.980 4.200 7.116 0.500 7.198 1.200 7.268 2.800 7.345 0.500 7.436 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 34 Return to Lane Navigator
3.500 13.600 4.550 7.100 5.200 6.400 5.850 12.700 6.550 7.300 6.850 3.900 7.350 5.800 8.150 7.100 8.450 5.600 8.650 6.200 9.300 24.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.500 8.300 3.876 1.200 4.148 11.500 4.323 4.100 4.550 4.100 4.772 1.000 4.856 0.200 5.108 0.100 5.200 3.800 5.262 3.600 5.319 1.600 5.395 2.400 5.499 0.300 5.655 1.500 5.779 6.300 5.850 2.900 5.888 6.100 5.981 3.000 6.015 1.700 6.050 1.000 6.154 2.000 6.300 2.100 6.396 1.300 6.446 1.000 6.550 2.200 6.688 0.100 6.850 1.700 7.027 1.100 7.113 0.600 7.151 0.500 7.226 1.400 7.350 4.400 7.501 1.300 8.150 4.800 8.450 2.300 8.650 2.200 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 14 PeakCount: 37 Return to Lane Navigator
3.802 2.600 4.178 18.400 4.359 7.800 4.624 2.200 4.805 1.900 4.883 0 4.945 0.100 5.016 0.800 5.102 0.600 5.173 0.700 5.272 12.500 5.328 3.100 5.393 1.900 5.501 0.600 5.575 0.200 5.626 1.600 5.757 9.900 5.873 6.600 5.972 2.700 6.003 2.500 6.037 1.800 6.133 3.400 6.286 3.300 6.366 1.300 6.428 2.100 6.582 0.600 6.952 4.000 7.096 0.900 7.171 0.500 7.230 3.800 7.326 0.300 7.445 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 15 PeakCount: 32 Return to Lane Navigator
3.500 15.800 4.550 7.300 5.200 6.900 5.850 10.900 6.550 8.200 6.850 3.800 7.350 7.400 8.150 6.200 8.450 5.000 8.650 4.700 9.300 23.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 16 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.