|
M87
Lysine Decarboxylase Broth
(Falkow)
(for Salmonella)
Gelysate or peptone | 5 g |
Yeast extract | 3 g |
Glucose | 1 g |
L-Lysine | 5 g |
Bromcresol purple | 0.02 g |
Distilled water | 1 liter |
Heat until dissolved. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2.
For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21