Heat with agitation to dissolve. Autoclave 15 min at 121°C. Cool to 50°C.

Egg yolk emulsion

To each 500 ml of melted liver veal agar, add 40 ml egg yolk-saline suspension (see M51 for instructions). Mix thoroughly and pour into sterile 15 x 100 mm petri dishes. Dry plates at room temperature for 2 days or at 35°C for 24 h. Check plates for sterility and store sterile plates in refrigerator. In certain instances the medium may be used without addition of egg yolk emulsion.

Hypertext updated by rim/cjm 2005-OCT-21