M81
Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium
|
M81
Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium
| Phenylethanol agar (Difco) | 35.5 g |
| Glycine anhydride ( NOTE: not glycine) |
10 g |
| Lithium chloride | 5 g |
| Moxalactam stock solution, 1% in phosphate buffer, pH 6.0 |
2 ml |
| Distilled water | 1 liter |
Sterilize medium (without moxalactam) at 121°C for 15 min. Cool to 48-50°C and add filter-sterilized moxalactam solution.
Consists of 1 g moxalactam salt (ammonium or sodium) in 100 ml 0.1 M potassium phosphate buffer, pH 6.0. Store filter-sterilized stock solution frozen in 2 ml aliquots.
Moxalactam (Eli Lilly Co.) is retailed by Sigma Chemical Co. The LPM medium is most effective in the Henry illumination system when poured thin, i.e., 12-15 ml per standard petri dish. To avoid drying of thin agar, refrigerate and use plates rapidly. LPM basal medium is commercially available as a powder.
Hypertext updated by rim/cjm 2005-OCT-21
