|
M80
Levine's Eosin-Methylene Blue (L-EMB) Agar
Peptone | 10 g |
Lactose | 10 g |
K2HPO4 | 2 g |
Agar | 15 g |
Eosin Y | 0.4 g |
Methylene blue | 0.065 g |
Distilled water | 1 liter |
Boil to dissolve peptone, phosphate, and agar in 1 liter of water. Add water to make original volume. Dispense in 100 or 200 ml portions and autoclave 15 min at not over 121°C. Final pH, 7.1 ± 0.2.
Before use, melt, and to each 100 ml portion add:
When using complete dehydrated product, boil to dissolve all ingredients in 1 liter water. Dispense in 100 or 200 ml portions and autoclave 15 min at 121°C. Final pH, 7.1 ± 0.2.
Hypertext updated by rim/cjm 2005-OCT-21